Nuova stazione di Utricularia sp. (Lentibulariaceae) per il Modenese

The presence of Utricularia sp. was observed in a pond during a survey in some small water bodies in the Modena Apennines (Italy). This ecosystem was sampled and observed in order to apply the IBEM index, which allows judging the quality of these habitats on the basis of their biodiversity. A specific taxonomic determination was not possible because of the lack of the flower on the individuals; nevertheless, some features led to U. australis. However, it was decided to report the new finding since all species of the genus Utricularia have become quite rare in Italy

Invasive alien species of European Union concern: the use of a faunistic database for the knowledge and future management at a local scale

A research group of the Department of Life Sciences, University of Modena and Reggio Emilia, with the contribution of the Province of Modena and the Emilia-Romagna Region, implemented a faunistic database named Darwin. The database gathers information chiefly on the vertebrate fauna of the province of Modena (Northern Italy). To date, over 20,000 historical and recent records have been collected, regarding 535 vertebrate species and 7 invertebrates (Bivalvia and Crustacea) of high managing interest. Darwin allows to register the modification of the local fauna, with regards to the most problematic taxa and especially to the allochthonous species, which are mainly organisms living in the lowlands that became invasive at different levels. Some of these assumed the role of key species in a variety of ecosystems (e.g., Pseudorasbora parva), some threaten certain autochthonous species already at risk (e.g., Trachemys scripta and Procambarus clarkii), others give rise to economic issues (e.g., Myocastor coypus), whilst the remaining ones have progressively lost their invasivity, becoming rare or sporadic (e.g., Ameiurus melas and Lepomis gibbosus). The database represents a strongly effective tool for the basic knowledge functional to the actions deriving from the European Regulation 2014/1143, regarding the recent “List of invasive alien species of Union concern” (EU 2016/1141). Overall, Darwin collects information on the local distribution of 32 invasive allochthonous species, 7 of which of Union concern, namely: Lithobates catesbeianus, Myocastor coypus, Procambarus clarkii, Pseudorasbora parva, Trachemys scripta, Threskiornis aethiopicus, and Oxyura jamaicensis. The first four species are established within the province, while T. scripta is diffused but not definitively confirmed as breeding, T. aethiopicus is present but not breeding, and O. jamaicensis is observed only occasionally. Thanks to the database the autochthonous species of EU interest comprised in the national IUCN Red list and their competitors could be brought into focus. For instance, we revealed the presence of merely 8 local populations of Emys orbicularis, threatened by the wide distribution of T. scripta, which is reported from over 20 sites within Modena’s territory. Furthermore, we found Austropotamobius pallipes at 122 mid-elevation sites (100-1100 m a.s.l.), whilst its direct competitor P. clarkii, currently expanding to the foothills, was recorded from 101 locations from 10 to 100 m a.s.l. These preliminary results highlight the necessity of control plans towards the two alien species, which could be developed on the basis of the already available distribution patterns. Our research group aims at the constant implementation of the database by adding information from ongoing and forthcoming studies on species of major interest. The existing data, presently used in various control plans, will pave the way to more specific investigations on the invasive alien fauna and increase the effectiveness of future managing actions

Il Lago della Ninfa (Appennino modenese): comunit\ue0 macrozoobentonica, fauna vertebrata e considerazioni ecologiche

The biocoenoses of Lake Ninfa in the Northern Apennines (Province of Modena, Italy) have undergone heavy tampering since 1961 when this small impoundment was completely emptied in order to allow the dredging of the lake bottom. Consequently, the original vegetation was uprooted and the impervious sediments of the lake floor removed. This study has taken into account the present situation of the lake\u2019s macrozoobenthos after previous investigations going back to some 30 years ago (Crema & Zunarelli Vandini, 1983). For this purpose, sampling was carried out in June, July and October 2011 and in the spring of 2014. At the same time, the main chemical and physical parameters were acquired and observations concerning the batrachofauna and ichthyofauna of this wetland were carried out

Generation and Characterization of a Tumor Stromal Microenvironment and Analysis of Its Interplay with Breast Cancer Cells: An In Vitro Model to Study Breast Cancer-Associated Fibroblast Inactivation

Breast cancer-associated fibroblasts (BCAFs), the most abundant non-cancer stromal cells of the breast tumor microenvironment (TME), dramatically sustain breast cancer (BC) progression by interacting with BC cells. BCAFs, as well as myofibroblasts, display an up regulation of activation and inflammation markers represented by α-smooth muscle actin (α-SMA) and cyclooxygenase 2 (COX-2). BCAF aggregates have been identified in the peripheral blood of metastatic BC patients. We generated an in vitro stromal model consisting of human primary BCAFs grown as monolayers or 3D cell aggregates, namely spheroids and reverted BCAFs, obtained from BCAF spheroids reverted to 2D cell adhesion growth after 216 h of 3D culture. We firstly evaluated the state of activation and inflammation and the mesenchymal status of the BCAF monolayers, BCAF spheroids and reverted BCAFs. Then, we analyzed the MCF-7 cell viability and migration following treatment with conditioned media from the different BCAF cultures. After 216 h of 3D culture, the BCAFs acquired an inactivated phenotype, associated with a significant reduction in α-SMA and COX-2 protein expression. The deactivation of the BCAF spheroids at 216 h was further confirmed by the cytostatic effect exerted by their conditioned medium on MCF-7 cells. Interestingly, the reverted BCAFs also retained a less activated phenotype as indicated by α-SMA protein expression reduction. Furthermore, the reverted BCAFs exhibited a reduced pro-tumor phenotype as indicated by the anti-migratory effect exerted by their conditioned medium on MCF-7 cells. The deactivation of BCAFs without drug treatment is possible and leads to a reduced capability of BCAFs to sustain BC progression in vitro. Consequently, this study could be a starting point to develop new therapeutic strategies targeting BCAFs and their interactions with cancer cells

A pan-European epidemiological study reveals honey bee colony survival depends on beekeeper education and disease control

Reports of honey bee population decline has spurred many national efforts to understand the extent of the problem and to identify causative or associated factors. However, our collective understanding of the factors has been hampered by a lack of joined up trans-national effort. Moreover, the impacts of beekeeper knowledge and beekeeping management practices have often been overlooked, despite honey bees being a managed pollinator. Here, we established a standardised active monitoring network for 5 798 apiaries over two consecutive years to quantify honey bee colony mortality across 17 European countries. Our data demonstrate that overwinter losses ranged between 2% and 32%, and that high summer losses were likely to follow high winter losses. Multivariate Poisson regression models revealed that hobbyist beekeepers with small apiaries and little experience in beekeeping had double the winter mortality rate when compared to professional beekeepers. Furthermore, honey bees kept by professional beekeepers never showed signs of disease, unlike apiaries from hobbyist beekeepers that had symptoms of bacterial infection and heavy Varroa infestation. Our data highlight beekeeper background and apicultural practices as major drivers of honey bee colony losses. The benefits of conducting trans-national monitoring schemes and improving beekeeper training are discussed

Control of paratuberculosis: who, why and how. A review of 48 countries

Paratuberculosis, a chronic disease affecting ruminant livestock, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). It has direct and indirect economic costs, impacts animal welfare and arouses public health concerns. In a survey of 48 countries we found paratuberculosis to be very common in livestock. In about half the countries more than 20% of herds and flocks were infected with MAP. Most countries had large ruminant populations (millions), several types of farmed ruminants, multiple husbandry systems and tens of thousands of individual farms, creating challenges for disease control. In addition, numerous species of free-living wildlife were infected. Paratuberculosis was notifiable in most countries, but formal control programs were present in only 22 countries. Generally, these were the more highly developed countries with advanced veterinary services. Of the countries without a formal control program for paratuberculosis, 76% were in South and Central America, Asia and Africa while 20% were in Europe. Control programs were justified most commonly on animal health grounds, but protecting market access and public health were other factors. Prevalence reduction was the major objective in most countries, but Norway and Sweden aimed to eradicate the disease, so surveillance and response were their major objectives. Government funding was involved in about two thirds of countries, but operations tended to be funded by farmers and their organizations and not by government alone. The majority of countries (60%) had voluntary control programs. Generally, programs were supported by incentives for joining, financial compensation and/or penalties for non-participation. Performance indicators, structure, leadership, practices and tools used in control programs are also presented. Securing funding for long-term control activities was a widespread problem. Control programs were reported to be successful in 16 (73%) of the 22 countries. Recommendations are made for future control programs, including a primary goal of establishing an international code for paratuberculosis, leading to universal acknowledgment of the principles and methods of control in relation to endemic and transboundary disease. An holistic approach across all ruminant livestock industries and long-term commitment is required for control of paratuberculosis

An improved GPU-oriented algorithm for elastostatic analysis with Boundary Element Method

A parallel, Boundary Element Method procedure for elastostatic problems is here illustrated. Starting from a reference collocation-integration algorithm, a preliminary study, mainly devoted to the performance analysis of the method has been first carried out. Subsequently, three main areas that offer potential improvements have been detected. Several enhanced versions of the algorithm, incorporating the suggested modifications, have been tested and numerically characterized on test-cases with varying boundary conditions and mesh density. Finally, a parallel, high-efficiency, GPU-based version, able to process large domains fully featured with internal solutions has been developed and discussed in terms of cost/benefit ratio and overall performance

Segnalazioni floristiche per la provincia di Modena

Durante i sopralluoghi in 19 zone umide dell’Appennino modenese per campionamenti ed osservazioni inerenti l’applicazione dell’Indice de Biodiversité des Étangs et Mares (IBEM; Angélibert et al., 2010; Indermuehle et al., 2010), che consente di dare un giudizio di qualità di questi biotopi basandosi sulla loro biodiversità, è stata osservata la presenza di Typha shuttleworthii W.D.J. Koch & Sond. (Typhaceae) e di Utricularia sp. (Lentibulariaceae). La prima, non ancora segnalata per il modenese, è presente nello stagno della Rovinella (Montecreto) in associazione con T. latifolia. Grazie a questo ritrovamento tutte e cinque le specie appartenenti al genere Typha incluse nella Flora d’Italia (Pignatti, 1982) sono da considerarsi presenti nel territorio modenese