Nitrogen and Phosphorus Signaling and Transport During Legume–Rhizobium Symbiosis

Nitrogen (N) and phosphorus (P) are the two predominant mineral elements, which are not only essential for plant growth and development in general but also play a key role in symbiotic N fixation in legumes. Legume plants have evolved complex signaling networks to respond to both external and internal levels of these macronutrients to optimize symbiotic N fixation in nodules. Inorganic phosphate (Pi) and nitrate (NO3−) are the two major forms of P and N elements utilized by plants, respectively. Pi starvation and NO3− application both reduce symbiotic N fixation via similar changes in the nodule gene expression and invoke local and long-distance, systemic responses, of which N-compound feedback regulation of rhizobial nitrogenase activity appears to operate under both conditions. Most of the N and P signaling and transport processes have been investigated in model organisms, such as Medicago truncatula, Lotus japonicus, Glycine max, Phaseolus vulgaris, Arabidopsis thaliana, Oryza sativa, etc. We attempted to discuss some of these processes wherever appropriate, to serve as references for a better understanding of the N and P signaling and transport during symbiosis

The role for CYCLIN A1;2/TARDY ASYNCHRONOUS MEIOSIS in differentiated cells in Arabidopsis

The Arabidopsis A1-type cyclin, CYCA1;2, also named TARDY ASYNCHRONOUS MEIOSIS (TAM), is known for its positive role in meiotic cell cycle progression, but its function in other cells has not been characterized. Here we report the role of TAM in cell differentiation in vegetative organs. The expression pattern of TAM was investigated by promoter and protein fusions using the β- glucuronidase (GUS) and the green fluorescent protein (GFP), respectively. Based on the expression pattern, morphologies of cells expressing TAM were studied in various plants including loss-of-function tam-1 and tam-2 mutants, a TAM-overexpression line, tam-1 complemented by the wild type TAM, and the wild-type. In addition, ploidy profiles in mature leaves of these plants were determined by flow cytometric analysis. TAM expression was found primarily in non-proliferating cells such as guard cells, trichomes, and mesophyll cells, and in vascular tissue, but its protein is kept at low levels. The tam mutants exhibited a reduction in trichome branch number but had a normal ploidy profile in the leaves. Compared to the wild type, these mutants also exhibited more pavement cell swelling, and the null allele, tam-2, had a larger average guard cell size but a normal ploidy level in the guard cells. Overexpression of TAM produced phenotypic changes opposite to those in the loss-of-function mutants, in addition to an increase in the relative portion of diploid cells. These results suggest that the primary role of TAM in vegetative organs is to regulate cell differentiation or cell turgidity in a cell-cycle-independent manner.Plant Biology, Ecology and Evolutio

SoC-Cluster as an Edge Server: an Application-driven Measurement Study

Huge electricity consumption is a severe issue for edge data centers. To this end, we propose a new form of edge server, namely SoC-Cluster, that orchestrates many low-power mobile system-on-chips (SoCs) through an on-chip network. For the first time, we have developed a concrete SoC-Cluster server that consists of 60 Qualcomm Snapdragon 865 SoCs in a 2U rack. Such a server has been commercialized successfully and deployed in large scale on edge clouds. The current dominant workload on those deployed SoC-Clusters is cloud gaming, as mobile SoCs can seamlessly run native mobile games. The primary goal of this work is to demystify whether SoC-Cluster can efficiently serve more general-purpose, edge-typical workloads. Therefore, we built a benchmark suite that leverages state-of-the-art libraries for two killer edge workloads, i.e., video transcoding and deep learning inference. The benchmark comprehensively reports the performance, power consumption, and other application-specific metrics. We then performed a thorough measurement study and directly compared SoC-Cluster with traditional edge servers (with Intel CPU and NVIDIA GPU) with respect to physical size, electricity, and billing. The results reveal the advantages of SoC-Cluster, especially its high energy efficiency and the ability to proportionally scale energy consumption with various incoming loads, as well as its limitations. The results also provide insightful implications and valuable guidance to further improve SoC-Cluster and land it in broader edge scenarios

Brown Carbon Aerosol in Urban Xi’an, Northwest China: TheComposition and Light Absorption Properties

Light-absorbing organic carbon (i.e., brown carbon or BrC) in the atmospheric aerosol has significant contribution to light absorption and radiative forcing. However, the link between BrC optical properties and chemical composition remains poorly constrained. In this study, we combine spectrophotometric measurements and chemical analyses of BrC samples collected from July 2008 to June 2009 in urban Xi'an, Northwest China. Elevated BrC was observed in winter (5 times higher than in summer), largely due to increased emissions from wintertime domestic biomass burning. The light absorption coefficient of methanol-soluble BrC at 365 nm (on average approximately twice that of water-soluble BrC) was found to correlate strongly with both parent polycyclic aromatic hydrocarbons (parent-PAHs, 27 species) and their carbonyl oxygenated derivatives (carbonyl-OPAHs, 15 species) in all seasons (r(2) > 0.61). These measured parent-PAHs and carbonyl-OPAHs account for on average similar to 1.7% of the overall absorption of methanol-soluble BrC, about 5 times higher than their mass fraction in total organic carbon (OC, similar to 0.35%). The fractional solar absorption by BrC relative to element carbon (EC) in the ultraviolet range (300-400 nm) is significant during winter (42 +/- 18% for water-soluble BrC and 76 +/- 29% for methanol-soluble BrC), which may greatly affect the radiative balance and tropospheric photochemistry and therefore the climate and air quality

The Medicago truncatula nodule‐specific cysteine‐rich peptides, NCR343 and NCR‐new35 are required for the maintenance of rhizobia in nitrogen‐fixing nodules

In the nodules of IRLC legumes, including Medicago truncatula, nitrogen-fixing rhizobia undergo terminal differentiation resulting in elongated and endoreduplicated bacteroids specialized for nitrogen fixation. This irreversible transition of rhizobia is mediated by host produced nodule-specific cysteine-rich (NCR) peptides, of which c. 700 are encoded in the M. truncatula genome but only few of them have been proved to be essential for nitrogen fixation. We carried out the characterization of the nodulation phenotype of three ineffective nitrogen-fixing M. truncatula mutants using confocal and electron microscopy, monitored the expression of defence and senescence-related marker genes, and analysed the bacteroid differentiation with flow cytometry. Genetic mapping combined with microarray- or transcriptome-based cloning was used to identify the impaired genes. Mtsym19 and Mtsym20 mutants are defective in the same peptide NCR-new35 and the lack of NCR343 is responsible for the ineffective symbiosis of NF-FN9363. We found that the expression of NCR-new35 is significantly lower and limited to the transition zone of the nodule compared with other crucial NCRs. The fluorescent protein-tagged version of NCR343 and NCR-new35 localized to the symbiotic compartment. Our discovery added two additional members to the group of NCR genes essential for nitrogen-fixing symbiosis in M. truncatula

Light Plays an Essential Role in Intracellular Distribution of Auxin Efflux Carrier PIN2 in Arabidopsis thaliana

BACKGROUND: Light plays a key role in multiple plant developmental processes. It has been shown that root development is modulated by shoot-localized light signaling and requires shoot-derived transport of the plant hormone, auxin. However, the mechanism by which light regulates root development is not largely understood. In plants, the endogenous auxin, indole-3-acetic acid, is directionally transported by plasma-membrane (PM)-localized auxin influx and efflux carriers in transporting cells. Remarkably, the auxin efflux carrier PIN proteins exhibit asymmetric PM localization, determining the polarity of auxin transport. Similar to PM-resident receptors and transporters in animal and yeast cells, PIN proteins undergo constitutive cycling between the PM and endosomal compartments. Auxin plays multiple roles in PIN protein intracellular trafficking, inhibiting PIN2 endocytosis at some concentrations and promoting PIN2 degradation at others. However, how PIN proteins are turned over in plant cells is yet to be addressed. METHODOLOGY AND PRINCIPLE FINDINGS: Using laser confocal scanning microscopy, and physiological and molecular genetic approaches, here, we show that in dark-grown seedlings, the PM localization of auxin efflux carrier PIN2 was largely reduced, and, in addition, PIN2 signal was detected in vacuolar compartments. This is in contrast to light-grown seedlings where PIN2 was predominantly PM-localized. In light-grown plants after shift to dark or to continuous red or far-red light, PIN2 also accumulated in vacuolar compartments. We show that PIN2 vacuolar targeting was derived from the PM via endocytic trafficking and inhibited by HY5-dependent light signaling. In addition, the ubiquitin 26S proteasome is involved in the process, since its inhibition by mutations in COP9 and a proteasome inhibitor MG132 impaired the process. CONCLUSIONS AND SIGNIFICANCE: Collectively, our data indicate that light plays an essential role in PIN2 intracellular trafficking, promoting PM-localization in the presence of light and, on the other hand, vacuolar targeting for protein degradation in the absence of light. Based on these results, we postulate that light regulation of root development is mediated at least in part by changes in the intracellular distribution of auxin efflux carriers, PIN proteins, in response to the light environment

Space charge behavior in multi-layer oil-paper insulation under different DC voltages and temperatures

Oil-paper as a reliable insulation system is widely used in power transformers and cables. The dielectric properties of oil-paper insulation play an important role in the reliable operation of power equipment. However, the formation and dynamics of space charge can affect the performance of insulation material. In this paper, space charge in oil-paper insulation system has been investigated using the pulsed electroacoustic (PEA) technique. A series of measurements were carried out when the insulation system was subjected to different applied voltages at different temperatures. Charge behavior in the insulation system has been analyzed and the influence of temperature on charge dynamics was discussed. The test results show that homocharge injection takes place under all the test conditions, the applied dc voltage mainly effect the amount of space charge, while the temperature has greater influence on the distribution and mobility of space charge inside oil-paper sample

Compound leaves of Medicago truncatula natural variants

This is a set of images of compound leaves of 45-days-old Medicago truncatula wild type (Jemalong A17), and PI 516927, PI 517939 and PI 577609 natural variants. Clockwise from top left, L1 to L7

Regulation of Compound Leaf Development

Leaf morphology is one of the most variable, yet inheritable, traits in the plant kingdom. How plants develop a variety of forms and shapes is a major biological question. Here, we discuss some recent progress in understanding the development of compound or dissected leaves in model species, such as tomato (Solanum lycopersicum), Cardamine hirsuta and Medicago truncatula, with an emphasis on recent discoveries in legumes. We also discuss progress in gene regulations and hormonal actions in compound leaf development. These studies facilitate our understanding of the underlying regulatory mechanisms and put forward a prospective in compound leaf studies

LeafletAnalyzer, an Automated Software for Quantifying, Comparing and Classifying Blade and Serration Features of Compound Leaves during Development, and among Induced Mutants and Natural Variants in the Legume Medicago truncatula

Diverse leaf forms ranging from simple to compound leaves are found in plants. It is known that the final leaf size and shape vary greatly in response to developmental and environmental changes. However, changes in leaf size and shape have been quantitatively characterized only in a limited number of species. Here, we report development of LeafletAnalyzer, an automated image analysis and classification software to analyze and classify blade and serration characteristics of trifoliate leaves in Medicago truncatula. The software processes high quality leaf images in an automated or manual fashion to generate size and shape parameters for both blades and serrations. In addition, it generates spectral components for each leaflets using elliptic Fourier transformation. Reconstruction studies show that the spectral components can be reliably used to rebuild the original leaflet images, with low, and middle and high frequency spectral components corresponding to the outline and serration of leaflets, respectively. The software uses artificial neutral network or k-means classification method to classify leaflet groups that are developed either on successive nodes of stems within a genotype or among genotypes such as natural variants and developmental mutants. The automated feature of the software allows analysis of thousands of leaf samples within a short period of time, thus facilitating identification, comparison and classification of leaf groups based on leaflet size, shape and tooth features during leaf development, and among induced mutants and natural variants