Low f-number microlenses for integration on optical microsystems

This paper presents microlenses (MLs) with low f-number made of AZ4562 photoresist for integration on optical microsystems. The fabrication process was based on the thermal reflow and rehydration. Large series of MLs were fabricated with a width of 35 μm, a thickness of 5 μm, and spaced apart by 3 μm. The MLs were fabricated directly on the surface of a die with type n+/p-substrate junction photodiode fabricated in a standard CMOS process. The measured focal length was 49 μm with a tolerance of ±2 μm (maximum error of ±4%), resulting in a numerical aperture of 33.6 × 10-2 (±1.3 × 10-2). The measurements also revealed an f-number of 1.4.This work was sponsored by the Brazilian agency Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) under the grant 400110/2014-8: Ciencia sem Fronteiras, Bolsa Pesquisador Visitante Especial (PVE) 2014. The associate editor coordinating the review of this paper and approving it for publication was Prof. M. Nurul Abedin

Anaerobic biodegradability of category 2 animal by-products : methane potential and inoculum source

Category 2 animal by-products that need to be sterilized with steam pressure according Regulation (EC) 1774/2002 are studied. In this work, 2 sets of experiments were performed in mesophilic conditions: (i) biomethane potential determination testing 0.5%, 2.0% and 5.0% total solids (TS), using sludge from the anaerobic digester of a wastewater treatment plant as inoculum; (ii) biodegradability tests at a constant TS concentration of 2.0% and different inoculum sources (digested sludge from a wastewater treatment plant; granular sludge from an upflow anaerobic sludge blanket reactor; leachate from a municipal solid waste landfill; and sludge from the slaughterhouse wastewater treatment anaerobic lagoon) to select the more adapted inoculum to the substrate in study. The higher specific methane production was of 317 mL CH4 g−1 VSsubstrate for 2.0% TS. The digested sludge from the wastewater treatment plant led to the lowest lag-phase period and higher methane potential rate.This work was partially supported by project PEst-C/EQB/LA0020/2011, financed by FEDER through COMPETE - Programa Operacional Factores de Competitividade and by FCT - Fundacao para a Ciencia e a Tecnologia. FCT is acknowledged for the financial support given to Tatiana Pozdniakova through the PhD scholarship (SFRH/BD/45144/2008) and Jose Carlos Costa through the grant (SFRH/BDP/48962/2008)

αvβ3 and α5β1 integrin-specific ligands: From tumor angiogenesis inhibitors to vascularization promoters in regenerative medicine?

Integrins are cell adhesion receptors predominantly important during normal and tumor angiogenesis. A sequence present on several extracellular matrix proteins composed of Arg-Gly-Asp (RGD) has attracted attention due to its role in cell adhesion mediated by integrins. The development of ligands that can bind to integrins involved in tumor angiogenesis and brake disease progression has resulted in new investigational drug entities reaching the clinical trial phase in humans. The use of integrin-specific ligands can be useful for the vascularization of regenerative medicine constructs, which remains a major limitation for translation into clinical practice. In order to enhance vascularization, immobilization of integrin-specific RGD peptidomimetics within constructs is a recommended approach, due to their high specificity and selectivity towards certain desired integrins. This review endeavours to address the potential of peptidomimetic-coated biomaterials as vascular network promoters for regenerative medicine purposes. Clinical studies involving molecules tracking active integrins in cancer angiogenesis and reasons for their failure are also addressed.Prémios Santa Casa Neurociências - Prize Melo e Castro for Spinal Cord Injury Research; Portuguese Foundation for Science and Technology [Doctoral fellowship (PD/BDE/127835/2016) to L. A. Rocha; IF Development Grant to A. J. Salgado; national funds through grant TUBITAK/0007/2014]. This article has been developed under the scope of the projects NORTE-01-0145-FEDER-000013, supported by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER); This work has been funded by FEDER funds, through the Competitiveness Factors Operational Programme (COMPETE), and by National funds, through the Foundation for Science and Technology (FCT), under the scope of the project POCI-01-0145-FEDER-007038info:eu-repo/semantics/publishedVersio

Synergistic Effects of Ocean Warming and Cyanide Poisoning in an Ornamental Tropical Reef Fish

UIDP/50017/2020 UIDB/50017/2020 UID/MAR/04292/2019 SFRH/BPD/117491/2016 SFRH/BPD/115298/2016 SFRH/BPD/99819/2014 PD/BD/52568/2014An array of anthropogenic pressures is affecting tropical ecosystems, posing major conservation challenges for scientists, stakeholders and populations. Illegal cyanide fishing is one of the major threats to Indo-Pacific coral reefs, targeting a multitude of colorful species for the marine aquarium trade as well as large-sized groupers and wrasses for the food fish trade. Ultimately, the continued use of this destructive practice as oceans warm may overload tropical ecosystems and result in irreversible ecological damage. Here we show that the impact of cyanide poisoning in an ornamental tropical marine fish is magnified under increased temperatures. A sole pulse exposure of 60 s to 50 mg L–1 of cyanide under current temperature (26°C) caused substantial mortality (50–100%) in eight species of Pomacentridae. The clownfish Amphiprion ocellaris was the most resistant, especially medium-sized fish [average total length and weight of 38 mm and 1.12 g; LC50 (95% CI) = 50.00 (46.76 − 53.24) mg L–1] that showed shorter recovery times and higher survival rates (%) when compared to small-sized ones [average total length and weight of 25 mm and 0.30 g; LC50 (95% CI) = 28.45 (20.17 − 36.72) mg L–1]. However, when the most resistant size-class was concomitantly exposed to a sub-lethal dosage of cyanide (25 mg L–1 instead of 50 mg L–1) and ocean warming scenarios for 2100 (+3°C and heat wave +6°C), survival rates (%) decreased to 60 and 20%, respectively, and recovery times increased in the worst case scenario. Mortality outbreaks, as well as vulnerability to predation, will likely expand in fish inhabiting coral reefs exposed to cyanide fishing unless stronger conservation measures are taken in tropical reefs to limit this destructive practice now and in the oceans of tomorrow.publishersversionpublishe

A fuzzified systematic adjustment of the robotic Darwinian PSO

The Darwinian Particle Swarm Optimization (DPSO) is an evolutionary algorithm that extends the Particle Swarm Optimization using natural selection to enhance the ability to escape from sub-optimal solutions. An extension of the DPSO to multi-robot applications has been recently proposed and denoted as Robotic Darwinian PSO (RDPSO), benefiting from the dynamical partitioning of the whole population of robots, hence decreasing the amount of required information exchange among robots. This paper further extends the previously proposed algorithm adapting the behavior of robots based on a set of context-based evaluation metrics. Those metrics are then used as inputs of a fuzzy system so as to systematically adjust the RDPSO parameters (i.e., outputs of the fuzzy system), thus improving its convergence rate, susceptibility to obstacles and communication constraints. The adapted RDPSO is evaluated in groups of physical robots, being further explored using larger populations of simulated mobile robots within a larger scenario

Are the effects induced by increased temperature enhanced in Mytilus galloprovincialis submitted to air exposure?

Intertidal mussel species are frequently exposed to changes of environmental parameters related to tidal regimes that include a multitude of stressors that they must avoid or tolerate by developing adaptive strategies. In particular, besides air exposure during low tides, intertidal mussels are also subjected to warming and, consequently, to higher risk of desiccation. However, scarce information is available regarding the responses of mussels to tidal regimes, particularly in the presence of other stressors such as increased temperature. Investigating the impacts of such combination of conditions will allow to understand the possible impacts that both factors interaction may generate to these intertidal organisms. To this end, the present study evaluated the impacts of different temperatures (18 ºC and 21 ºC) on Mytilus galloprovincialis when continuously submersed or exposed to a tidal regime for 14 days. Results showed that in mussels exposed to increased temperature under submersion conditions, the stress induced was enough to activate mussels’ antioxidant defenses (namely glutathione peroxidase, GPx), preventing oxidative damage (lipid peroxidation, LPO; protein carbonylation, PC). In mussels exposed to tides at control temperature, metabolic capacity increased (electron transport system activity, ETS), and GPx was induced, despite resulting in increased LPO levels. Moreover, the combination of tides and temperature increase led to a significant decrease of lipid (LIP) content, activation of antioxidant defenses (superoxide dismutase, SOD; GPx) and increase of oxidized glutathione (GSSG), despite these mechanisms were not sufficient to prevent increased cellular damage. Therefore, the combination of increased temperature and air exposure induced higher oxidative stress in mussels. These findings indicate that increasing global warming could be more impacting to intertidal organisms compared to organisms continuously submersed. Furthermore, our results indicate that air exposure can act as a confounding factor when assessing the impacts of different stressors in organisms living in coastal systemspublishe

Practical issues in the development of a minimalistic power management solution for WSNs

A flexible Wireless Sensor Network platform for implementation of diverse applications has been developed and deployed at Instituto Superior Técnico - Technical University of Lisbon (IST-TUL). Since its initial deployment in 2007, this testbed has grown steadily, supporting new nodes, applications and experiments. However, some initial problems, which were solved on an ad hoc basis, were becoming more serious as the network spanned throughout the campus. Major issues, like global power management, have to be tackled not only with traditional protocol level approaches but also from a system’s viewpoint, providing solutions capable of guaranteeing a consistent testbed. We discuss the main issues related with the development of power management solutions, presenting our architecture, design choices and implementation, and address the lessons learnt from its integration. Experimental evaluation of our solution has shown considerable energy savings, extending network lifetime by up to nine times

Modelação da dispersão longitudinal em sistemas fluviais : casos de estudo

O estudo da hidrodinâmica e dispersão de descargas poluentes em sistemas fluviais assume particular relevância no estabelecimento de esquemas de gestão integrada da água em bacias hidrográficas, visando a protecção dos ecossistemas aquáticos e tendo em consideração os usos aí existentes. O presente trabalho teve como objectivo a determinação de parâmetros capazes de traduzir as características dispersivas em trechos de dois sistemas fluviais distintos. Para esse efeito, foram realizadas campanhas de amostragem num trecho do Rio Mondego e numa albufeira internacional do Rio Douro, em que se recorreu à injecção de um marcador (rodamina WT). Os resultados obtidos serviram de base à calibração e validação de um modelo de advecção-difusão com capacidade para descrever a evolução da concentração de substâncias conservativas nesses meios hídricos. Neste artigo apresenta-se a metodologia utilizada, os resultados experimentais obtidos numa das campanhas de amostragem, a atenuação dos picos de concentração em função dos tempos de percurso e a sua comparação com os resultados dos modelos matemáticos de simulação do fenómeno de dispersão longitudinal

Tracer experiments for dispersion modelling in river Douro water quality management

Parameters estimation for in situ dispersion river water behaviour characterisation, and performance evaluation of numerical models, applied to reproduce data field obtained from dye tracer experiments, were the main purposes of this research work. A monitoring program was carried out using tracer injection to assess the environmental impact of accidental pollutant discharges in an international reach of river Douro, between Castro dam (Spain) and Miranda dam (Portugal). This paper presents the methodology used in the tracer experiments, the concentration-time curves, describing dye tracer spread, the results of different simulation and their agreement with the measured data, and peak attenuation with dye spread travel time. Longitudinal dispersion coefficients values are estimated according to the river hydrodynamics

Comparision of numerical techniques solving longitudinal dispersion problems in the river Mondego

Judicious selection of mathematical models for application in a specific river basin management can mitigate prediction uncertainty. Therefore, intervention times will be established with better reliability and alarm systems could efficiently protect the aquatic ecosystems and the public health. The main purpose of this paper is to evaluate the performance of different numerical techniques when applied to river water systems dispersion modelling. A case study was developed to assess the environmental impact of Urgeiriça mining waters in a Mondego river reach, between Caldas da Felgueira and Aguieira reservoir. A monitoring program was carried out using tracer injection (rhodamine WT) to determine the in situ dispersion river water behaviour. The present work describes the methodology used in the tracer experiments, presents the concentration-time curves obtained and the performance of finite difference method (FDM) and finite element method (FEM) in a simplified river system, and compares the results of different numerical techniques application for longitudinal dispersion coefficient estimation in this river reach. The application of DUFLOW package, that includes hydrodynamics and water quality models, showed the best agreement with experimental data, allowing a reasonable support for impact assessment of different discharges scenarios in the river water quality