Single-tube multiplex real-time PCR with EvaGreen and high-resolution melting analysis for diagnosis of α0-thalassemia--SEA,--THAI, and--CR type deletions.

Regions with a high prevalence of α-thalassemia (α-thal) require simple, rapid, and accurate tests for carrier screening and prenatal diagnosis. Diagnosis of multiple deletions in a single tube is necessary to clearly identify individuals with α0-thalassemia in the routine setting, especially in at-risk couples. Therefore, we aimed to develop a single-tube multiplex real-time PCR with EvaGreen and high-resolution melting (HRM) analysis for the identification of α0-thalassemia Southeast Asian (SEA), Thai and Chiang Rai (CR) type deletions. The results of the HRM analysis indicated that the amplified fragments from α0-thal--CR,--THAI,--SEA, and the wild-type α-globin gene had specific peak heights at mean melting temperature (Tm) values of 85.40°C, 86.50°C, 87.65°C, and 91.04°C, respectively. The frequencies of α0-thal--SEA,--THAI,--CR obtained from routine testing in 2,135 samples were 17.89%, 0.19% and 0.19%, respectively. This method would be useful for preventing Hb Bart's hydrops fetalis. Detection of multiple deletions in a single run is cost-effective, highly accurate and timesaving. This technique could enable wider α-thalassemia diagnosis in high prevalence areas and served as an example for thalassemia routine setting

Health Issues in Medical Students: How We Need To Take Care of Our Future Generation Doctors

Medical profession is one of the most coveted professions in the society. During the medical course, individuals are subjected to much stress and hardships. Many fail to cope up and quit the course or even land up in substance abuse. A major cause of concern is the health of any medico. On few occasion the disease may be imaginative in nature whereas at other times it may be actually present. There is a need to differentiate between two. At many occasions, there are illnesses which are a cause of worry for both parents and administration. The health of every single budding doctor is important as entrance to medical course is an intellectual superior individual it incurs heavy expenses on the part of the administration and we cannot afford to compromise on such a potential asset to the society. We performed an extensive search of literature such as Pubmed, Scopus and Google for obtaining necessary articles for this short narrative mini review. Search terms included: “medical”, students”, “illnesses”, disease.” The present review is first of its kind which aims to discuss the medical illness and various diseases affecting the medicos during their professional career. The results of the review may help in better planning in terms of taking care of the health of medical student