Tumour-specific microRNA expression pattern in canine intestinal T-cell-lymphomas

Intestinal T-cell lymphomas are common in dogs, but histopathological diagnosis remains challenging because of accompanying enteritis with lymphocyte involvement. Invasively taken full-layer biopsies are still required for reliable differentiation. The detection of specific microRNA expression patterns in canine intestinal T-cell lymphoma could provide new possibilities to differ intestinal lymphoma from benign inflammation and could lead to further understanding of lymphomagenesis. The objective of this study was to characterize microRNA expression in distinct groups of formalin-fixed and paraffin-embedded samples from canine intestinal T-cell lymphomas, lymphoplasmacellular enteritis and healthy intestinal tissue. In a preliminary test with two samples per group, total RNA was extracted (RNEasy FFPE Kit, Qiagen), reverse transcribed (miScript II RT Kit, Qiagen) and pre-amplified (miScript PreAmp PCR Kit, Qiagen). We performed comparative quantitative PCR on microRNA PCR Array plates (Qiagen) with pre-fabricated reactions for 183 different mature canine microRNAs. Subsequently, 12 microRNAs with conspicuous expression changes in the lymphoma group were selected and microRNA expression of all samples (n = 8) per group was analysed with individual microRNA assays (miScript Primer Assays, Qiagen) on the reverse transcribed RNA without pre-amplification. Our results revealed lymphoma-specific expression patterns, with down-regulation of the tumour-suppressing microRNAs miR-194, miR-192, miR-141 and miR-203, and up-regulation of oncogenic microRNAs, including microRNAs from the miR-106a~363 cluster. In addition, we detected only slight expression alterations between healthy intestinal tissue and lymphoplasmacellular enteritis cases. We conclude that microRNA expression patterns can be used to separate T-cell lymphomas from healthy tissue and benign inflammatory disorders

Time delays to reach dispatch centres in different regions in Europe. Are we losing the window of opportunity? - The EUROCALL study

Aim: In out of hospital cardiac arrest (OHCA) the start of Cardiopulmonary Resuscitation (CPR) by a single rescuer may be delayed considerably if the total time (TT) to connect the telephone call to the Emergency Medical Communication Centre (EMCC) is prolonged. EUROCALL investigated the TT-EMCC and its components using different calling procedures. Methods: This prospective, multicentre, randomised study was performed in April 2013. Telephone calls were randomly allocated to time of call, and to those connecting directly to the EMCC (1-step procedure) and those diverted before connecting to the EMCC (2-step procedure). Results: Twenty-one EMCCs from 11 countries participated in the study. Time to first ringtone was similar between 1-step 3.7 s (IQR 1.0-5.2) and 2-step calls 4.0 s (IQR 2.4-5.2). For the 1878 1-step calls, the median TT-EMCC was 11.7 s (IQR 8.7-18.5). For the 1550 2-step calls, the median time from first ringtone to first call-taker was 7 s (IQR 4.6-11.9) and from first call-taker to EMCC was 18.7 s (IQR 13.4-29.9). Median TT-EMCC was 33.2 s (IQR 24.7-46.1) and was significantly longer than the TT-EMCC observed with the 1-step procedure (P <0.0001). Significant differences existed among participating regions between and within different countries both for 1-step and 2-step procedures. Conclusion: TT-EMCC was significantly shorter in a 1-step procedure compared to a 2-step procedure. Regional differences existed between countries but also within countries. This may be relevant in cases of OHCA and other situations where patient outcome is critically time-dependent. (C) 2016 Elsevier Ireland Ltd. All rights reserved.Peer reviewe

Concert recording 2018-10-29

[Track 1]. The wolf\u27s aria from Little red riding hood / Seymour Barab -- [Track 2]. Die beiden Grenadiene / Robert Schumann -- [Track 3]. Jeanie with the light brown hair / Stephen Foster -- [Track 4]. Verborgenheit / Hugo Wolf -- [Track 5]. Ridente la calma / Wolfgang Amadeus Mozart -- [Track 6]. Allerseelen / Richard Strauss -- [Track 7]. Laurie\u27s song from The tender land / Aaron Copland -- [Track 8]. Come raggio di sol / Antonio Caldara -- [Track 9]. Gretchen am Spinnrade / Franz Schubert -- [Track 10]. La Spectre de la Rose from Les Nuits d\u27Ete / Hector Berlioz -- [Track 11]. Lied der Mignon / Franz Schubert -- [Track 12]. This is my beloved from Kismet / Robert Wright George Forrest -- [Track 13]. Dein blaues Auge [Track 14]. Wie Melodien zieht es / Johannes Brahms -- [Track 15]. The infinite shining heavens from Songs of travel / Ralph Vaughan-Williams -- [Track 16]. O stay, my love / Sergei Rachmaninoff -- [Track 17]. Il lacerato spirito from Simon Boccanegra / Giuseppe Verdi -- [Track 18]. There but for you I go from Brigadoon / Lerner and Loewe

Concert recording 2017-10-02

[Track 1]. Amarilli bella / Giulio Caccini -- [Track 2]. El sueño imposible from Man of La Mancha / Joe Darion -- [Track 3]. An Sylvia / Franz Schubert -- [Track 4]. O Isis und Osiris / Wolfgang Amadeus Mozart -- [Track 5]. Apres un reve / Gabriel Fauré -- [Track 6]. Dido\u27s lament from Dido and Aeneas / Henry Purcell -- [Track 7]. This in my beloved from Kismet / Robert Wright -- [Track 8]. Heidenröslein / Franz Schubert -- [Track 9]. Lord God of Abraham from Elijah / Felix Mendelssohn -- [Track 10]. Lieben, hassen, hoffen, sagen / Richard Strauss -- [Track 11]. Auch kleine Dinge from Italienischers Liederbuch / Hugo Wolf -- [Track 12]. Saper vorreste from Un ballo in maschera / Giuseppe Verdi -- [Track 13]. Ombra mai fu from Xerxes / George Frideric Handel -- [Track 14]. You\u27ll never walk alone from Carousel / Richard Rodgers and Oscar Hammerstein II -- [Track 15]. Les berceaux / Fauré -- [Track 16]. I remember from Evening primrose / Stephen Sondheim -- [Track 17]. Vaghissima sembianza / Stefano Donaudy -- [Track 18]. Moonfall from The mystery of Edwin Drood / Rupert Holmes -- [Track 19]. Stranger in paradise from Kismet / Robert Wright and George Forrest -- [Track 20]. Morire / Giacomo Puccini -- [Track 21]. Sorge infausta una procella from Orlando / Handel -- [Track 22]. Vi ravviso from La sonnambula / Vincenzo Bellini

First-response treatment after out-of-hospital cardiac arrest:a survey of current practices across 29 countries in Europe

Background: In Europe, survival rates after out-of-hospital cardiac arrest (OHCA) vary widely. Presence/absence and differences in implementation of systems dispatching First Responders (FR) in order to arrive before Emergency Medical Services (EMS) may contribute to this variation. A comprehensive overview of the different types of FR-systems used across Europe is lacking. Methods: A mixed-method survey and information retrieved from national resuscitation councils and national EMS services were used as a basis for an inventory. The survey was sent to 51 OHCA experts across 29 European countries. Results: Forty-seven (92%) OHCA experts from 29 countries responded to the survey. More than half of European countries had at least one region with a FR-system. Four categories of FR types were identified: (1) firefighters (professional/voluntary); (2) police officers; (3) citizen-responders; (4) others including off-duty EMS personnel (nurses, medical doctors), taxi drivers. Three main roles for FRs were identified: (a) complementary to EMS; (b) part of EMS; (c) instead of EMS. A wide variation in FR-systems was observed, both between and within countries. Conclusions: Policies relating to FRs are commonly implemented on a regional level, leading to a wide variation in FR-systems between and within countries. Future research should focus on identifying the FR-systems that most strongly influence survival. The large variation in local circumstances across regions suggests that it is unlikely that there will be a 'one-size fits all' FR-system for Europe, but examining the role of FRs in the Chain of Survival is likely to become an increasingly important aspect of OHCA research

Solstice: An Electronic Journal of Geography and Mathematics, Volume XXII, Number 2.

This document was delivered over the internet. Look for animations and .kmz files for Google Earth as attachments within the pdf version of this journal. These appear naturally within the online html version. See the full html files, also located at: http://www.imagenet.org/The purpose of Solstice is to promote interaction between geography and mathematics. Articles in which elements of one discipline are used to shed light on the other are particularly sought. Also welcome, are original contributions that are purely geographical or purely mathematical. These may be prefaced (by editor or author) with commentary suggesting directions that might lead toward the desired interaction. Contributed articles will be refereed by geographers and/or mathematicians. Invited articles will be screened by suitable members of the editorial board. IMaGe is open to having authors suggest, and furnish material for, new regular features.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/88213/1/SolsticeDecember2011.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/88213/3/Koenig.pptxhttp://deepblue.lib.umich.edu/bitstream/2027.42/88213/4/Arlinghaus2011.pptxhttp://deepblue.lib.umich.edu/bitstream/2027.42/88213/5/BridgeOdyssey.kmzhttp://deepblue.lib.umich.edu/bitstream/2027.42/88213/6/BridgeOdysseyBlog.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/88213/7/VarroaGlobal.kmzhttp://deepblue.lib.umich.edu/bitstream/2027.42/88213/8/Sammataro2011.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/88213/9/AMOVirtualCemetery.kmzhttp://deepblue.lib.umich.edu/bitstream/2027.42/88213/10/ToblerLambertReview.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/88213/17/ArlinghausKoenig.kmzhttps://deepblue.lib.umich.edu/bitstream/2027.42/88213/18/SolsticeVolXXIINo2.pdf8Description of SolsticeDecember2011.pdf : Solstice, December 2011, journal coverDescription of Koenig.pptx : Contemporary Views Along the First Transcontinental Railroad, KoenigDescription of Arlinghaus2011.pptx : The Perimeter Project, Part 6. Arlinghaus and ArlinghausDescription of BridgeOdyssey.kmz : Bridge Odyssey of Craig Robinson, Google Earth file of S. ArlinghausDescription of BridgeOdysseyBlog.pdf : Bridge Odyssey, Blog. Robinson.Description of ArlinghausKoenig.kmz : 3D file with Koenig articleDescription of VarroaGlobal.kmz : Varroa Mite Project of Diana Sammataro. Google Earth file, S. Arlinghaus.Description of Sammataro2011.pdf : Varroa Mite Project, update. D. SammataroDescription of AMOVirtualCemetery.kmz : Virtual Cemetery project with W. E. Arlinghaus. Google Earth file. S. ArlinghausDescription of ToblerLambertReview.pdf : Pdf capture of animated review of Lambert/Tobler documentDescription of SolsticeVolXXIINo2.pdf : Cover of Journa

BRCA2 polymorphic stop codon K3326X and the risk of breast, prostate, and ovarian cancers

Background: The K3326X variant in BRCA2 (BRCA2*c.9976A&gt;T; p.Lys3326*; rs11571833) has been found to be associated with small increased risks of breast cancer. However, it is not clear to what extent linkage disequilibrium with fully pathogenic mutations might account for this association. There is scant information about the effect of K3326X in other hormone-related cancers. Methods: Using weighted logistic regression, we analyzed data from the large iCOGS study including 76 637 cancer case patients and 83 796 control patients to estimate odds ratios (ORw) and 95% confidence intervals (CIs) for K3326X variant carriers in relation to breast, ovarian, and prostate cancer risks, with weights defined as probability of not having a pathogenic BRCA2 variant. Using Cox proportional hazards modeling, we also examined the associations of K3326X with breast and ovarian cancer risks among 7183 BRCA1 variant carriers. All statistical tests were two-sided. Results: The K3326X variant was associated with breast (ORw = 1.28, 95% CI = 1.17 to 1.40, P = 5.9x10- 6) and invasive ovarian cancer (ORw = 1.26, 95% CI = 1.10 to 1.43, P = 3.8x10-3). These associations were stronger for serous ovarian cancer and for estrogen receptor–negative breast cancer (ORw = 1.46, 95% CI = 1.2 to 1.70, P = 3.4x10-5 and ORw = 1.50, 95% CI = 1.28 to 1.76, P = 4.1x10-5, respectively). For BRCA1 mutation carriers, there was a statistically significant inverse association of the K3326X variant with risk of ovarian cancer (HR = 0.43, 95% CI = 0.22 to 0.84, P = .013) but no association with breast cancer. No association with prostate cancer was observed. Conclusions: Our study provides evidence that the K3326X variant is associated with risk of developing breast and ovarian cancers independent of other pathogenic variants in BRCA2. Further studies are needed to determine the biological mechanism of action responsible for these associations