220 research outputs found

    Utility based cross-layer collaboration for speech enhancement in wireless acoustic sensor networks

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    A wireless acoustic sensor network is considered that is used to estimate a desired speech signal that has been corrupted by noise. The application layer of the WASN derives an optimal filter in a linear MMSE sense. A utility function is then used in conjunction with the MMSE estimate in order to evaluate the most significant signal components from each node in the system. The utility values are used as a cross-layer link between the application layer and the network layer so the nodes transmit the signal components that are deemed most relevant to the estimate while adhering to the power constraints of the system. The simulation results show that a high signal-to-error and signal-to-noise ratio is still achievable while transmitting a subset of signal components

    Weighted multivariate curve resolution – alternating least squares based on sample relevance

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    Alternating least squares, within the multivariate curve resolution framework has seen a lot of practical applications and shows its distinction with its relatively simple and flexible implementation. However, the limitations of least squares should be considered carefully when deviating from the standard assumed data structure. Within this work we highlight the effects of noise in the presence of minor components, and we propose a novel weighting scheme within the weighted multivariate curve-resolution-alternating least squares framework, to resolve it. Two simulated and one Raman imaging case is investigated, by comparing the novel methodology against standard multivariate curve resolution-alternating least squares and essential spectral pixel selection. A trade-off is observed between current methods, while the novel weighting scheme demonstrates a balance, where the benefits of the previous two methods are retained

    A unified radio control architecture for prototyping adaptive wireless protocols

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    Experimental optimization of wireless protocols and validation of novel solutions is often problematic, due to limited configuration space present in commercial wireless interfaces as well as complexity of monolithic driver implementation on SDR-based experimentation platforms. To overcome these limitations a novel software architecture is proposed, called WiSHFUL, devised to allow: i) maximal exploitation of radio functionalities available in current radio chips, and ii) clean separation between the logic for optimizing the radio protocols (i.e. radio control) and the definition of these protocols

    Vitamine A et infection pasteurellique expérimentale du porc. Fluctuations de l’activité cholinestérasique érythrocytaire et plasmatique

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    Ruckebusch Yves, Jean-Blain Marcel, Oudar Jean, Joubert L. Vitamine A et infection Pasteurellique expérimentale du Porc. Fluctuations de l’activité cholinestérasique érythrocytaire et plasmique. In: Bulletin de l'Académie Vétérinaire de France tome 113 n°7, 1960. pp. 391-397

    Frame-insensitive expression cloning of fluorescent protein from <i>Scolionema suvaense</i>

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    Expression cloning from cDNA is an important technique for acquiring genes encoding novel fluorescent proteins. However, the probability of in-frame cDNA insertion following the first start codon of the vector is normally only 1/3, which is a cause of low cloning efficiency. To overcome this issue, we developed a new expression plasmid vector, pRSET-TriEX, in which transcriptional slippage was induced by introducing a DNA sequence of (dT)14 next to the first start codon of pRSET. The effectiveness of frame-insensitive cloning was validated by inserting the gene encoding eGFP with all three possible frames to the vector. After transformation with one of these plasmids, E. coli cells expressed eGFP with no significant difference in the expression level. The pRSET-TriEX vector was then used for expression cloning of a novel fluorescent protein from Scolionema suvaense. We screened 3658 E. coli colonies transformed with pRSET-TriEX containing Scolionema suvaense cDNA, and found one colony expressing a novel green fluorescent protein, ScSuFP. The highest score in protein sequence similarity was 42% with the chain c of multi-domain green fluorescent protein like protein “ember” from Anthoathecata sp. Variations in the N- and/or C-terminal sequence of ScSuFP compared to other fluorescent proteins indicate that the expression cloning, rather than the sequence similarity-based methods, was crucial for acquiring the gene encoding ScSuFP. The absorption maximum was at 498 nm, with an extinction efficiency of 1.17 × 105 M−1·cm−1. The emission maximum was at 511 nm and the fluorescence quantum yield was determined to be 0.6. Pseudo-native gel electrophoresis showed that the protein forms obligatory homodimers
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