1,265 research outputs found
Effects of cysteinyl-leukotriene receptors' antagonism by montelukast on lung mechanics and olfactory system histology in healthy mice.
Context: At variance with steroid administration, the possible effects of leukotrienes inhibition on basal respiratory mechanics and olfactory system function are still unclear.Objective: To investigate if interference with the leukotrienes activity may influence basal lung mechanics in healthy mammals, as well as the olfactory system.Materials and methods: We measured lung mechanics by the end-inflation occlusion method in control and in montelukast i.p. treated anaesthetised healthy mice (10 mg/kg/die for a week). A study of olfactory system histology was also conducted.Results: Elastance and resistive properties of the lung were not affected by montelukast, while a significant increment of lung hysteresis was observed. The analysis of olfactory system histology revealed no significant effects of montelukast compared to controls.Discussion and conclusions: Leukotrienes' antagonism does not affect respiratory mechanics in basal conditions, except for a hysteresis increment, which might counteract the inc..
Numerical and Experimental Dynamic Analysis of IC Engine Test Beds Equipped with Highly Flexible Couplings
Driveline components connected to internal combustion engines can be critically loaded by dynamic forces due to motion irregularity. In particular, flexible couplings used in engine test rig are usually subjected to high levels of torsional oscillations and time-varying torque. This could lead to premature failure of the test rig. In this work an effective methodology for the estimation of the dynamic behavior of highly flexible couplings in real operational conditions is presented in order to prevent unwanted halts. The methodology addresses a combination of numerical models and experimental measurements. In particular, two mathematical models of the engine test rig were developed: a torsional lumped-parameter model for the estimation of the torsional dynamic behavior in operative conditions and a finite element model for the estimation of the natural frequencies of the coupling. The experimental campaign addressed torsional vibration measurements in order to characterize the driveline dynamic behavior as well as validate the models. The measurements were achieved by a coder-based technique using optical sensors and zebra tapes. Eventually, the validated models were used to evaluate the effect of design modifications of the coupling elements in terms of natural frequencies (torsional and bending), torsional vibration amplitude, and power loss in the couplings
When Just One Phosphate Is One Too Many: The Multifaceted Interplay between Myc and Kinases
Myc transcription factors are key regulators of many cellular processes, with Myc target genes crucially implicated in the management of cell proliferation and stem pluripotency, energy metabolism, protein synthesis, angiogenesis, DNA damage response, and apoptosis. Given the wide involvement of Myc in cellular dynamics, it is not surprising that its overexpression is frequently associated with cancer. Noteworthy, in cancer cells where high Myc levels are maintained, the overexpression of Myc-associated kinases is often observed and required to foster tumour cells' proliferation. A mutual interplay exists between Myc and kinases: the latter, which are Myc transcriptional targets, phosphorylate Myc, allowing its transcriptional activity, highlighting a clear regulatory loop. At the protein level, Myc activity and turnover is also tightly regulated by kinases, with a finely tuned balance between translation and rapid protein degradation. In this perspective, we focus on the cross-regulation of Myc and its associated protein kinases underlying similar and redundant mechanisms of regulation at different levels, from transcriptional to post-translational events. Furthermore, a review of the indirect effects of known kinase inhibitors on Myc provides an opportunity to identify alternative and combined therapeutic approaches for cancer treatment
A versatile facility for the calibration of X-ray polarimeters with polarized and unpolarized controlled beams
We devised and built a versatile facility for the calibration of the next
generation X-ray polarimeters with unpolarized and polarized radiation. The
former is produced at 5.9 keV by means of a Fe55 radioactive source or by X-ray
tubes, while the latter is obtained by Bragg diffraction at nearly 45 degrees.
Crystals tuned with the emission lines of X-ray tubes with molybdenum, rhodium,
calcium and titanium anodes are employed for the efficient production of highly
polarized photons at 2.29, 2.69, 3.69 and 4.51 keV respectively. Moreover the
continuum emission is exploited for the production of polarized photons at 1.65
keV and 2.04 keV and at energies corresponding to the higher orders of
diffraction. The photons are collimated by means of interchangeable capillary
plates and diaphragms, allowing a trade-off between collimation and high
fluxes. The direction of the beam is accurately arranged by means of high
precision motorized stages, controlled via computer so that long and automatic
measurements can be done. Selecting the direction of polarization and the
incidence point we can map the response of imaging devices to both polarized
and unpolarized radiation. Changing the inclination of the beam we can study
the systematic effects due to the focusing of grazing incidence optics and the
feasibility of instruments with large field of view.Comment: 12 pages, 11 figure
Identification of Possible Pre-Slaughter Indicators to Predict Stress and Meat Quality: A Study on Heavy Pigs
This study aimed at identifying possible pre-slaughter indicators and/or indexes to be used to predict pig stress response and meat quality variation. Data were collected on 44 shipments (loads) of Italian heavy pigs. For each shipment, several pre-slaughter parameters were recorded on farm, during transport, and at the slaughterhouse. Blood and meat samples were taken from 10 pigs from every of the 44 loads included in the study (N = 440). Blood samples were used to assess cortisol and creatine kinase levels, whereas meat samples were used to assess meat quality (pH, instrumental color, tenderness, water-holding capacity, and sensory analysis). Cluster analysis of blood parameters allowed the categorization of the shipments into two main clusters: Lower Stress (LS) and Higher Stress (HS). The variables/indexes statistically differing between the two clusters were: average vehicle speed during transport, welfare index at slaughter (i.e., \u201cslaughter score\u201d), overall transport and slaughter welfare index (TSWI), distance travelled, and behaviors (slips, falls, overlaps) during unloading, which appeared to be the best descriptors of the welfare conditions experienced by Italian heavy pigs during pre-slaughter handling. No consistent effects of the stress level experienced on meat quality was detected, which warrants the need for further studies conducted under more variable pre-slaughter conditions, with the aim of simplifying and improving the TSWI
An X-ray Polarimeter for HXMT Mission
The development of micropixel gas detectors, capable to image tracks produced
in a gas by photoelectrons, makes possible to perform polarimetry of X-ray
celestial sources in the focus of grazing incidence X-ray telescopes. HXMT is a
mission by the Chinese Space Agency aimed to survey the Hard X-ray Sky with
Phoswich detectors, by exploitation of the direct demodulation technique. Since
a fraction of the HXMT time will be spent on dedicated pointing of particular
sources, it could host, with moderate additional resources a pair of X-ray
telescopes, each with a photoelectric X-ray polarimeter in the focal plane. We
present the design of the telescopes and the focal plane instrumentation and
discuss the performance of this instrument to detect the degree and angle of
linear polarization of some representative sources. Notwithstanding the limited
resources the proposed instrument can represent a breakthrough in X-ray
Polarimetry.Comment: 10 pages, 9 figure
Re-testing the JET-X Flight Module No. 2 at the PANTER facility
The Joint European X-ray Telescope (JET-X) was the core instrument of the
Russian Spectrum-X-gamma space observatory. It consisted of two identical soft
X-ray (0.3 - 10 keV) telescopes with focusing optical modules having a measured
angular resolution of nearly 15 arcsec. Soon after the payload completion, the
mission was cancelled and the two optical flight modules (FM) were brought to
the Brera Astronomical Observatory where they had been manufactured. After 16
years of storage, we have utilized the JET-X FM2 to test at the PANTER X-ray
facility a prototype of a novel X-ray polarimetric telescope, using a Gas Pixel
Detector (GPD) with polarimetric capabilities in the focal plane of the FM2.
The GPD was developed by a collaboration between INFN-Pisa and INAF-IAPS. In
the first phase of the test campaign, we have re-tested the FM2 at PANTER to
have an up-to-date characterization in terms of angular resolution and
effective area, while in the second part of the test the GPD has been placed in
the focal plane of the FM2. In this paper we report the results of the tests of
the sole FM2, using an unpolarized X-ray source, comparing the results with the
calibration done in 1996.Comment: Author's accepted manuscript posted to arXiv.org as permitted by
Springer's Self-Archiving Policy. The final publication is available at
http://rd.springer.com/article/10.1007%2Fs10686-013-9365-
In vitro biological effects of raw and thermally treated asbestos-containing materials
Asbestos cement, the main asbestos-containing material (ACM) manufactured in Italy in the past, is a health hazard whose elimination is a priority concern. Asbestos fibers can be transformed into potentially non-hazardous silicates by high-temperature treatment via complete solid-state transformation. In this study human A549 cells were directly exposed to raw cement asbestos (RCA), chrysotile and cement asbestos subjected to an industrial process at 1200 °C (HT-CA) and raw commercial grey cement (GC) for 24 and 48h, or treated with conditioned culture medium up to 96 h. In our previous studies we demonstrated that the final product of heat treatment of cement asbestos was considerably more inert and had lower cytotoxic potential than the original asbestos material. However, to better evaluate the risks of interactions with the materials, further in vitro investigations were performed concerning fiber-cell superficial interactions, immuno-hystochemical expression of cytochines p53, p53 homologue p73, TNF-related apoptosis- inducing ligand (TRAIL), and conditioned medium effects on cell viability. Data showed more severe cytotoxic damage by raw cement-asbestos compared to the heat treated materials and different expressions of cytochines that exert critical role in regulating the cell response to asbestos-induced DNA damage. These data should be taken in consideration for a safe recycling of thermal transformed asbestos materials
The Gas Pixel Detector as an X-ray photoelectric polarimeter with a large field of view
The Gas Pixel Detector (GPD) is a new generation device which, thanks to its
50 um pixels, is capable of imaging the photoelectrons tracks produced by
photoelectric absorption in a gas. Since the direction of emission of the
photoelectrons is strongly correlated with the direction of polarization of the
absorbed photons, this device has been proposed as a polarimeter for the study
of astrophysical sources, with a sensitivity far higher than the instruments
flown to date. The GPD has been always regarded as a focal plane instrument and
then it has been proposed to be included on the next generation space-borne
missions together with a grazing incidence optics. Instead in this paper we
explore the feasibility of a new kind of application of the GPD and of the
photoelectric polarimeters in general, i.e. an instrument with a large field of
view. By means of an analytical treatment and measurements, we verify if it is
possible to preserve the sensitivity to the polarization for inclined beams,
opening the way for the measurement of X-ray polarization for transient
astrophysical sources. While severe systematic effects arise for inclination
greater than about 20 degrees, methods and algorithms to control them are
discussed.Comment: 11 pages, 8 figure
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