Thyroxine is the serum factor that regulates morphogenesis of columnar cartilage from isolated chondrocytes in chemically defined medium.

Epiphyseal chondrocytes cultured in a medium containing 10% serum may be maintained as three dimensional aggregates and differentiate terminally into hypertrophic cells. There is an attendant expression of genes encoding type X collagen and high levels of alkaline phosphatase activity. Manipulation of the serum concentration to optimal levels of 0.1 or 0.01% in this chondrocyte pellet culture system results in formation of features of developing cartilage architecture which have been observed exclusively in growth cartilage in vivo. Cells are arranged in columns radiating out from the center of the tissue, and can be divided into distinct zones corresponding to the recognized stages of chondrocyte differentiation. Elimination of the optimal serum concentration in a chemically defined medium containing insulin eliminates the events of terminal differentiation of defined cartilage architecture. Chondrocytes continue to enlarge into hypertrophic cells and synthesize type X collagen mRNA and protein, but in the absence of the optimal serum concentration, alkaline phosphatase activity does not increase and the cells retain a random orientation. Addition of thyroxine to the chemically defined medium containing insulin and growth hormone results in dose-dependent increases in both type X collagen synthesis and alkaline phosphatase activity, and reproduces the optimal serum-induced morphogenesis of chondrocytes into a columnar pattern. These experiments demonstrate the critical role of thyroxine in cartilage morphogenesis

The Advocate: Should He Speak or Write?

This speech was given by The Rt. Hon. Lord Mackay on April 3, 1991, at Fordham University\u27s School of Law as the John F. Sonnet Memorial Lecture

Construction of periodic adapted orthonormal frames on closed space curves

The construction of continuous adapted orthonormal frames along C1 closed–loop spatial curves is addressed. Such frames are important in the design of periodic spatial rigid–body motions along smooth closed paths. The construction is illustrated through the simplest non–trivial context — namely, C1 closed loops defined by a single Pythagorean–hodograph (PH) quintic space curve of a prescribed total arc length. It is shown that such curves comprise a two–parameter family, dependent on two angular variables, and they degenerate to planar curves when these parameters differ by an integer multiple of π. The desired frame is constructed through a rotation applied to the normal–plane vectors of the Euler–Rodrigues frame, so as to interpolate a given initial/final frame orientation. A general solution for periodic adapted frames of minimal twist on C1 closed–loop PH curves is possible, although this incurs transcendental terms. However, the C1 closed–loop PH quintics admit particularly simple rational periodic adapted frames

Hierarchical incremental class learning with reduced pattern training

Hierarchical Incremental Class Learning (HICL) is a new task decomposition method that addresses the pattern classification problem. HICL is proven to be a good classifier but closer examination reveals areas for potential improvement. This paper proposes a theoretical model to evaluate the performance of HICL and presents an approach to improve the classification accuracy of HICL by applying the concept of Reduced Pattern Training (RPT). The theoretical analysis shows that HICL can achieve better classification accuracy than Output Parallelism [1]. The procedure for RPT is described and compared with the original training procedure. RPT reduces systematically the size of the training data set based on the order of sub-networks built. The results from four benchmark classification problems show much promise for the improved model

A computer model of auditory efferent suppression: Implications for the recognition of speech in noise

The neural mechanisms underlying the ability of human listeners to recognize speech in the presence of background noise are still imperfectly understood. However, there is mounting evidence that the medial olivocochlear system plays an important role, via efferents that exert a suppressive effect on the response of the basilar membrane. The current paper presents a computer modeling study that investigates the possible role of this activity on speech intelligibility in noise. A model of auditory efferent processing [ Ferry, R. T., and Meddis, R. (2007). J. Acoust. Soc. Am. 122, 3519?3526 ] is used to provide acoustic features for a statistical automatic speech recognition system, thus allowing the effects of efferent activity on speech intelligibility to be quantified. Performance of the ?basic? model (without efferent activity) on a connected digit recognition task is good when the speech is uncorrupted by noise but falls when noise is present. However, recognition performance is much improved when efferent activity is applied. Furthermore, optimal performance is obtained when the amount of efferent activity is proportional to the noise level. The results obtained are consistent with the suggestion that efferent suppression causes a ?release from adaptation? in the auditory-nerve response to noisy speech, which enhances its intelligibility

Transductive Learning with String Kernels for Cross-Domain Text Classification

For many text classification tasks, there is a major problem posed by the lack of labeled data in a target domain. Although classifiers for a target domain can be trained on labeled text data from a related source domain, the accuracy of such classifiers is usually lower in the cross-domain setting. Recently, string kernels have obtained state-of-the-art results in various text classification tasks such as native language identification or automatic essay scoring. Moreover, classifiers based on string kernels have been found to be robust to the distribution gap between different domains. In this paper, we formally describe an algorithm composed of two simple yet effective transductive learning approaches to further improve the results of string kernels in cross-domain settings. By adapting string kernels to the test set without using the ground-truth test labels, we report significantly better accuracy rates in cross-domain English polarity classification.Comment: Accepted at ICONIP 2018. arXiv admin note: substantial text overlap with arXiv:1808.0840

Interferon as a macrophage activating factor. I. Enhancement of cytotoxicity by fresh and matured human monocytes in the absence of other soluble signals

The cytolytic activity ofhuman peripheral blood monocytes in vitro against K-562 human leukaemic target cells was stimulated by human fibroblast (fi-) and leucocyte (a-) interferon (IFN). Stimulation was by up to several times the corresponding control activity, and was observed with freshly isolated monocytes, and with monocytes cultured for various periods up to 10 days. The cytolytic activity of untreated monocytes was detectable at very low effector: target ratios ( < 5: 1), and fell between days 1 and 4 in culture, normally rising again towards the initial activity at day 8; this pattern was also observed when IFN was present continuously, although the activities were then always higher than in the corresponding control cells. Cytolysis showed a lag of about 6 hr, in contrast to that by natural killer (NK) cells, and was routinely measured over 24 hr. The course of stimulation by IFN and its dose-response were studied. Stimulation required the presence of IFN for at least 24 hr, and was maximal with between 1,000 and 10,000 units of IFN/ml. When IFN containing media were removed and replaced with control media, the monocyte activity remained stimulated for at least 4 days. Stimulation by fl-IFN was blocked by a specific antibody to fl-IFN, under conditions in which assayable IFN activity was also neutralized. Several control experiments indicated that the action of IFN was on the monocytes and not on the target cells. The morphological maturation of monocytes was retarded by IFN, even in cultures containing up to 50% serum. The effectiveness of fibroblast IFN indicated that stimulation could not be attributed to the lymphokines which might contaminate a-IFN. The action of IFN did not require simultaneous or antecedent in vitro stimulation by endotoxin. This was indicated both by serum free experiments, and also by others in which polymixin B was used to complex with and render unavailable any endotoxin present. Endotoxin showed an independent stimulatory effect, which could be prevented by polymixin

Characteristics of a 9mm triple-beam tuning fork resonant sensor

This paper describes the design and testing of the first miniaturised metallic triple-beam tuning fork resonant sensors for use in force, pressure and torque measurement applications. The new devices with 9mm length vibrating tines have resulted in over a 40% reduction in size when compared to previously tested resonators. The four fold increase in operating frequency to 26 kHz, with Q factors in air up to 4000, provides additional benefits for resolution, accuracy, range and overload capability. Measurement repeatability of at least 0.02% of span levels for torque transducers employing the sensors are quoted. Results of characterisation over the temperature range -30oC to +90oC are given

The Advocate: Should He Speak or Write?

This speech was given by The Rt. Hon. Lord Mackay on April 3, 1991, at Fordham University\u27s School of Law as the John F. Sonnet Memorial Lecture