INFLUENCING HEALTHY FOOD CHOICES IN THE MARINE CORPS

The purpose of this thesis is to create an influence campaign focused on improving nutritional choices of Marines across the Marine Corps. It explores the most current publications from the U.S. Departments of Agriculture and Health and Human Services, and the U.S. military to establish a healthy nutrition baseline and identify how the Marine Corps attempts to provide adequate nutrition to its Marines. It demonstrates how optimizing food nutrition equates to combat readiness of the force. It investigates how food affects the body and how nutrients and timing affect a tactical athlete's performance. Since Marines have to choose from the food selections provided, the thesis outlines principles of influence that are and/or could be used to change food behavior. Influencing Marines’ behavior in favor of healthier food types that optimize physical performance and longevity is a pursuit worthy of an organization that must be fit and ready for combat. The thesis culminates with an influence campaign outlining suggestions that would achieve this nutritional increase, and thus combat readiness, in the Marine Corps.Captain, United States Marine CorpsApproved for public release. Distribution is unlimited

Locate and mitigate the state of nitrate: Assessing potential sources of nutrients in tributaries to the Nooksack River

Anthropogenic activities greatly increase the amount of nitrogen entering our environment. While this allows for increased agriculture production, excess nitrogen raises health concerns for humans and ecosystems. Understanding the sources of excess nutrients is necessary for effective efforts to reduce them. We aimed to understand nutrient fluxes through tributaries to the Nooksack River, in particular, to what extent are excess nutrients arriving to northern Whatcom County from Canada via both surface and ground water. We collected water samples from three creeks fed by the central portion of the Sumas-Blaine aquifer (Kamm, Fishtrap, and Bertrand), two situated at the southern edge of the aquifer (Tenmile and Deer), and one located outside the aquifer (Schell). In our lab, we analyzed them for concentrations of nitrate, phosphate, and organic phosphorous. Phosphate and organic phosphorous concentrations were relatively low across streams (less than 0.093 mg/L-phosphate and less than 0.079 mg/L- organic phosphorous) while nitrate levels varied more. The highest average concentrations of nitrate occurred in the three creeks closest to the Canada-US border, Kamm Creek (6.05 mg/L), Fishtrap Creek (3.07 mg/L), and Bertrand Creek (1.85 mg/L). Schell Creek’s nitrate concentration was intermediate at 1.36 mg/L while Tenmile and Deer creeks had the lowest concentrations at 0.41 mg/L and 0.47 mg/L, respectively. Based on our results, groundwater contamination flowing southward from Canada may be contributing to the higher levels of nitrate observed in Kamm, Fishtrap, and Bertrand creeks. Comparatively, Tenmile, Deer, and Schell creeks may have lower nutrient concentrations in their stream water as a consequence of their greater distance from Canada. However, other factors such as different land use practices around creeks could impact nutrient concentrations and influence our results. Analyzing nutrient levels in groundwater of the Sumas-Blaine aquifer would help us better understand the relative contributions of local and Canadian nitrate contamination sources

The human silent information regulator (Sir)2 homologue hSIRT3 is a mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase.

The yeast silent information regulator (Sir)2 protein links cellular metabolism and transcriptional silencing through its nicotinamide adenine dinucleotide (NAD)-dependent histone deacetylase activity. We report that mitochondria from mammalian cells contain intrinsic NAD-dependent deacetylase activity. This activity is inhibited by the NAD hydrolysis product nicotinamide, but not by trichostatin A, consistent with a class III deacetylase. We identify this deacetylase as the nuclear-encoded human Sir2 homologue hSIRT3, and show that hSIRT3 is located within the mitochondrial matrix. Mitochondrial import of hSIRT3 is dependent on an NH2-terminal amphipathic alpha-helix rich in basic residues. hSIRT3 is proteolytically processed in the mitochondrial matrix to a 28-kD product. This processing can be reconstituted in vitro with recombinant mitochondrial matrix processing peptidase (MPP) and is inhibited by mutation of arginines 99 and 100. The unprocessed form of hSIRT3 is enzymatically inactive and becomes fully activated in vitro after cleavage by MPP. These observations demonstrate the existence of a latent class III deacetylase that becomes catalytically activated upon import into the human mitochondria

Biophysical properties of presynaptic short-term plasticity in hippocampal neurons: insights from electrophysiology, imaging and mechanistic models

Hippocampal neurons show different types of short-term plasticity (STP). Some exhibit facilitation of their synaptic responses and others depression. In this review we discuss presynaptic biophysical properties behind heterogeneity in STP in hippocampal neurons such as alterations in vesicle priming and docking, fusion, neurotransmitter filling and vesicle replenishment. We look into what types of information electrophysiology, imaging and mechanistic models have given about the time scales and relative impact of the different properties on STP with an emphasis on the use of mechanistic models as complementary tools to experimental procedures. Taken together this tells us that it is possible for a multitude of different mechanisms to underlie the same STP pattern, even though some are more important in specific cases, and that mechanistic models can be used to integrate the biophysical properties to see which mechanisms are more important in specific cases of STP

PigReuse: A Reuse-based Optimizer for Pig Latin

Pig Latin is a popular language which is widely used for parallel processing of massive data sets. Currently, subexpressions occurring repeatedly in Pig Latin scripts are executed as many times as they appear, and the current Pig Latin optimizer does not identify reuse opportunities.We present a novel optimization approach aiming at identifying and reusing repeated subexpressions in Pig Latin scripts. Our optimization algorithm, named PigReuse, operates on a particular algebraic representation of Pig Latin scripts. PigReuse identifies subexpression merging opportunities, selects the best ones to execute based on a cost function, and reuses their results as needed in order to compute exactly the same output as the original scripts. Our experiments demonstrate the effectiveness of our approach

The grounded psychometric development and initial validation of the Health Literacy Questionnaire (HLQ)

BACKGROUND: Health literacy has become an increasingly important concept in public health. We sought to develop a comprehensive measure of health literacy capable of diagnosing health literacy needs across individuals and organisations by utilizing perspectives from the general population, patients, practitioners and policymakers. METHODS: Using a validity-driven approach we undertook grounded consultations (workshops and interviews) to identify broad conceptually distinct domains. Questionnaire items were developed directly from the consultation data following a strict process aiming to capture the full range of experiences of people currently engaged in healthcare through to people in the general population. Psychometric analyses included confirmatory factor analysis (CFA) and item response theory. Cognitive interviews were used to ensure questions were understood as intended. Items were initially tested in a calibration sample from community health, home care and hospital settings (N=634) and then in a replication sample (N=405) comprising recent emergency department attendees. RESULTS: Initially 91 items were generated across 6 scales with agree/disagree response options and 5 scales with difficulty in undertaking tasks response options. Cognitive testing revealed that most items were well understood and only some minor re-wording was required. Psychometric testing of the calibration sample identified 34 poorly performing or conceptually redundant items and they were removed resulting in 10 scales. These were then tested in a replication sample and refined to yield 9 final scales comprising 44 items. A 9-factor CFA model was fitted to these items with no cross-loadings or correlated residuals allowed. Given the very restricted nature of the model, the fit was quite satisfactory: χ(2)(WLSMV)(866 d.f.) = 2927, p<0.000, CFI = 0.936, TLI = 0.930, RMSEA = 0.076, and WRMR = 1.698. Final scales included: Feeling understood and supported by healthcare providers; Having sufficient information to manage my health; Actively managing my health; Social support for health; Appraisal of health information; Ability to actively engage with healthcare providers; Navigating the healthcare system; Ability to find good health information; and Understand health information well enough to know what to do. CONCLUSIONS: The HLQ covers 9 conceptually distinct areas of health literacy to assess the needs and challenges of a wide range of people and organisations. Given the validity-driven approach, the HLQ is likely to be useful in surveys, intervention evaluation, and studies of the needs and capabilities of individuals

Coordinated community structure among trees, fungi and invertebrate groups in Amazonian rainforests

Little is known regarding how trophic interactions shape community assembly in tropical forests. Here we assess multi-taxonomic community assembly rules using a rare standardized coordinated inventory comprising exhaustive surveys of five highly-diverse taxonomic groups exerting key ecological functions: trees, fungi, earthworms, ants and spiders. We sampled 36 1.9-ha plots from four remote locations in French Guiana including precise soil measurements, and we tested whether species turnover was coordinated among groups across geographic and edaphic gradients. All species group pairs exhibited significant compositional associations that were independent from soil conditions. For some of the pairs, associations were also partly explained by soil properties, especially soil phosphorus availability. Our study provides evidence for coordinated turnover among taxonomic groups beyond simple relationships with environmental factors, thereby refining our understanding regarding the nature of interactions occurring among these ecologically important groups

Genomic island excisions in Bordetella petrii

<p>Abstract</p> <p>Background</p> <p>Among the members of the genus <it>Bordetella B. petrii </it>is unique, since it is the only species isolated from the environment, while the pathogenic Bordetellae are obligately associated with host organisms. Another feature distinguishing <it>B. petrii </it>from the other sequenced Bordetellae is the presence of a large number of mobile genetic elements including several large genomic regions with typical characteristics of genomic islands collectively known as integrative and conjugative elements (ICEs). These elements mainly encode accessory metabolic factors enabling this bacterium to grow on a large repertoire of aromatic compounds.</p> <p>Results</p> <p>During <it>in vitro </it>culture of <it>Bordetella petrii </it>colony variants appear frequently. We show that this variability can be attributed to the presence of a large number of metastable mobile genetic elements on its chromosome. In fact, the genome sequence of <it>B. petrii </it>revealed the presence of at least seven large genomic islands mostly encoding accessory metabolic functions involved in the degradation of aromatic compounds and detoxification of heavy metals. Four of these islands (termed GI1 to GI3 and GI6) are highly related to ICE<it>clc </it>of <it>Pseudomonas knackmussii </it>sp. strain B13. Here we present first data about the molecular characterization of these islands. We defined the exact borders of each island and we show that during standard culture of the bacteria these islands get excised from the chromosome. For all but one of these islands (GI5) we could detect circular intermediates. For the <it>clc</it>-like elements GI1 to GI3 of <it>B. petrii </it>we provide evidence that tandem insertion of these islands which all encode highly related integrases and attachment sites may also lead to incorporation of genomic DNA which originally was not part of the island and to the formation of huge composite islands. By integration of a tetracycline resistance cassette into GI3 we found this island to be rather unstable and to be lost from the bacterial population within about 100 consecutive generations. Furthermore, we show that GI3 is self transmissible and by conjugation can be transferred to <it>B. bronchiseptica </it>thus proving it to be an active integrative and conjugative element</p> <p>Conclusion</p> <p>The results show that phenotypic variation of <it>B. petrii </it>is correlated with the presence of genomic islands. Tandem integration of related islands may contribute to island evolution by the acquisition of genes originally belonging to the bacterial core genome. In conclusion, <it>B. petrii </it>appears to be the first member of the genus in which horizontal gene transfer events have massively shaped its genome structure.</p

Fungi of French Guiana gathered in a taxonomic, environmental and molecular dataset

International audienceIn Amazonia, the knowledge about Fungi remains patchy and biased towards accessible sites. This is particularly the case in French Guiana where the existing collections have been confined to few coastal localities. Here, we aimed at filling the gaps of knowledge in indersampled areas of this region, particularly focusing on the Basidiomycota. From 2011, we comprehensively collected fruiting-bodies with a stratified and reproducible sampling scheme in 126 plots. Sites of sampling reflected the main forest habitats of French Guiana in terms of soil fertility and topography. The dataset of 5219 specimens gathers 245 genera belonging to 75 families, 642 specimens are barcoded. The dataset is not a checklist as only 27% of the specimens are identified at the species level but 96% are identified at the genus level. We found an extraordinary diversity distributed across forest habitats. The dataset is an unprecedented and original collection of Basidiomycota for the region, making specimens available for taxonomists and ecologists. The database is publicly available in the GBIF repository (https://doi.org/10.15468/ymvlrp)