Additional file 3: Figure S3. of Autophagy is associated with chemoresistance in neuroblastoma

HCQ sensitizes NB cells to chemotherapy by inhibition of autophagy. A. N91-IGR or NB8-IGR cell viability was measured after vincristine or doxorubicin treatment combined or not to HCQ 30 µM. Results are expressed as percentage of corresponding control and represent mean ± SEM of 4 independent experiments. B. Autophagic activity of N91-IGR or NB8-IGR was measured using MDC agent after increasing concentration of vincristine or doxorubicin treatment combined or not to HCQ 30 µM. Fluorescence was quantified by spectrophotometer. (*: P < 0.05), (**: P < 0.01), (***: P < 0.001). (PDF 215 kb

Additional file 4: Figure S4. of Autophagy is associated with chemoresistance in neuroblastoma

Effect of ATG5 knockdown xenografts on tumor growth. (11/12) NSG Mice developed xenograft tumor from s.c injection of 5.106 sheGFP cells and only (3/12) from shATG5 cells (Table A). When tumor reached 200 mm3, mice were administered vincristine (0.4 mg/kg/day) or vehicle (saline) for 5 days. Progression of tumor volume was followed in each group. Each data point represents the mean 6 ± SE tumor volume for sheGFP cells and 1 or 2 for shAtg5 cells. At day 6, Tumors size from shATG5 cells were reduced compared with tumors from eGFP (B). Also, tumors from shATG5 cells were more sensitive to vincristine comparing to control cells. Immunohistochemistry was performed on the sections of tumors developed in the mouse model. with LC3 antibody and showed a high expression of LC3 II in control cells and a very low in cells ATG5 knockdown as well as treated (b, d) or not treated (a, c) (C). (PDF 259 kb