Quantum state engineering via unitary transformations

We construct a Hamiltonian for the generation of arbitrary pure states of the quantized electromagnetic field. The proposition is based upon the fact that a unitary transformation for the generation of number states has been already found. The general unitary transformation here obtained, would allow the use of nonlinear interactions for the production of pure states. We discuss the applicability of this method by giving examples of generation of simple superposition states. We also compare our Hamiltonian with the one resulting from the interaction of trapped ions with two laser fields.Comment: 5 pages in RevTeX, no figures, accepted for publication in Phys. Rev.

Cross-generational trans fat intake exacerbates UV radiation-induced damage in rat skin

AbstractWe evaluated the influence of dietary fats on ultraviolet radiation (UVR)-induced oxidative damage in skin of rats. Animals from two consecutive generations born of dams supplemented with fats during pregnancy and breastfeeding were maintained in the same supplementation: soybean-oil (SO, rich in n-6 FA, control group), fish-oil (FO, rich in n-3 FA) or hydrogenated-vegetable-fat (HVF, rich in TFA). At 90days of age, half the animals from the 2nd generation were exposed to UVR (0.25J/cm2) 3×/week for 12weeks. The FO group presented higher incorporation of n-3 FA in dorsal skin, while the HVF group incorporated TFA. Biochemical changes per se were observed in skin of the HVF group: greater generation of reactive oxygen species (ROS), lower mitochondrial integrity and increased Na+K+-ATPase activity. UVR exposure increased skin wrinkles scores and ROS generation and decreased mitochondrial integrity and reduced-glutathione levels in the HVF group. In FO, UVR exposure was associated with smaller skin thickness and reduced levels of protein–carbonyl, together with increased catalase activity and preserved Na+K+-ATPase function. In conclusion, while FO may be protective, trans fat may be harmful to skin health by making it more vulnerable to UVR injury and thus more prone to develop photoaging and skin cancer

Academic freedom in Europe: reviewing UNESCO’s recommendation

This paper examines the compliance of universities in the European Union with the UNESCO Recommendation concerning the Status of Higher–Education Teaching Personnel, which deals primarily with protection for academic freedom. The paper briefly surveys the European genesis of the modern research university and academic freedom, before evaluating compliance with the UNESCO recommendation on institutional autonomy, academic freedom, university governance and tenure. Following from this, the paper examines the reasons for the generally low level of compliance with the UNESCO Recommendation within the EU states, and considers how such compliance could be improved

Implementing mass rearing of trissolcus japonicus (Hymenoptera: Scelionidae) on cold-stored host eggs

Halyomorpha halys (Stål) (Hemiptera: Pentatomidae), a pest of Asian origin, has been causing severe damage to Italian agriculture. The application of classical biological control by the release of Trissolcus japonicus (Ashmead) (Hymenoptera: Scelionidae), an exotic egg parasitoid, appears to be one promising solution. In Italy, releases of T. japonicus in the field were authorized in 2020. In this study, some parameters that could influence the rearing of T. japonicus in insectaries were investigated. A significantly higher production of progeny was observed on host eggs stored at 6◦C (86.5%) compared to −24◦C (48.8%) for up to two months prior to exposure to parasitism. There were no significant differences in progeny production from single females in a vial provided with only one egg mass (83.2%) or 10 females inside a cage with 6 egg masses (83.9%). The exposure of parasitoids to refrigerated (6◦C) egg masses of H. halys for 72 h led to a significantly higher production of progeny (62.1%) compared to shorter exposures for 48 (44.0%) or 24 h (37.1%). A decline in production of progeny by the same female was detected between the first (62.1%) and the second parasitization (41.3%). Adult parasitoids stored at 16◦C for up to 90 days had an 87.1% survival rate, but a significant decrease in progeny production was detected. These parameters could be adjusted when rearing T. japonicus for specific aims such as the production of individuals for field release or colony maintenance

Tracing the dispersal route of the invasive Japanese beetle Popillia japonica

The Japanese beetle, Popillia japonica, is a highly polyphagous Scarabaeidae native to Japan that colonized North America and Azores in the last century and has recently invaded Italy and Switzerland. Considering its economic impact on the horticulture and turfgrass industries, this species was ranked within the EU priority pests list in 2019. According to the EU Convention on Biological Diversity, the identification of invasion routes is a pivotal aspect in an effective management program aimed at controlling invasive alien species. To reconstruct the source of introductions of this pest, we investigated the genetic variability of P. japonica in its native and invaded areas worldwide by analyzing 9 microsatellite loci and two mitochondrial genes, COX I and CytB. In its native area, P. japonica is structured into two populations: one in the southern and another in the northern-central region of Japan. A limited area within central Japan was identified as the putative source of the North American outbreak. Moreover, the ABC inference and phylogeographic reconstruction suggest that two European populations originated from two independent introductions. The Azores Islands outbreak occurred approximately 50 years ago and originated from the southeastern region of North America (For simplicity, in this paper North America refers to Canada and the USA), while the second introduction, more recently, occurred in Italy and Switzerland and originated from northeastern region of North America

Diagnosis of occult tuberculosis in hematological malignancy by enumeration of antigen-specific T cells.

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Um estudo da legislação e das métricas do setor elétrico brasileiro para fomentar a digitalização e a descentralização do mercado de energia

CIES2020 - XVII Congresso Ibérico e XIII Congresso Ibero-americano de Energia SolarRESUMO: A energia elétrica gerada de forma distribuída deve ser transacionada de forma segura e eficaz, o que pode ser obtido por meio da aplicação da tecnologia blockchain no setor elétrico. Objetiva-se com este artigo trabalhar com questões legais relacionadas ao setor de comercialização de energia elétrica indicando as necessidades de alterações na legislação e na regulamentação, a fim de possibilitar a aplicação da tecnologia blockchain. Assim como, objetiva-se propor métricas relacionadas à aplicação de blockchain no mercado de comercialização de energia elétrica brasileiro para promover a digitalização e o aprimoramento de seus processos. O estudo fundamenta-se e desenvolve-se por revisão documental do ambiente regulatório brasileiro; e por revisão documental e bibliográfica sobre o mercado de comercialização de energia elétrica. Os resultados são indicações de alterações na legislação e na regulamentação, e métricas de impactos para aplicação da tecnologia blockchain no mercado de comercialização de energia elétrica brasileiro.ABSTRACT: The electric energy generated in a distributed manner must be transacted safely and effectively. It can be obtained through the application of blockchain technology in the electric sector. The aim of this article is to work with legal issues related to the electric energy trading sector. It will be possible indicating the necessity for changes in legislation and regulation to enable the application of blockchain technology in this sector. As well, it aims to propose metrics related to the application of blockchain in the Brazilian electric energy trading market for the purpose of promote the digitization and improvement of its processes. This study is based and developed by documentary review of the Brazilian regulatory environment; and by documentary and bibliographic review about the electric energy commercialization market. The results are indications of changes in legislation and regulations, and metrics of impacts for the application of blockchain technology in the Brazilian electric energy commercialization market.info:eu-repo/semantics/publishedVersio

Recovering coherence from decoherence: a method of quantum state reconstruction

We present a feasible scheme for reconstructing the quantum state of a field prepared inside a lossy cavity. Quantum coherences are normally destroyed by dissipation, but we show that at zero temperature we are able to retrieve enough information about the initial state, making possible to recover its Wigner function as well as other quasiprobabilities. We provide a numerical simulation of a Schroedinger cat state reconstruction.Comment: 8 pages, in RevTeX, 4 figures, accepted for publication in Phys. Rev. A (november 1999

Late-Onset Sepsis Mortality among Preterm Infants: Beyond Time to First Antibiotics

Objective: To investigate the impact of timing, in vitro activity and appropriateness of empirical antimicrobials on the outcome of late-onset sepsis among preterm very low birth weight infants that are at high risk of developing meningitis. Study design: This retrospective study included 83 LOS episodes in 73 very low birth weight infants born at ≤32 weeks’ gestation with positive blood and/or cerebrospinal fluid culture or polymerase chain reaction at >72 h of age. To define the appropriateness of empirical antimicrobials we considered both their in vitro activity and their ideal delivery through the blood-brain barrier when meningitis was confirmed or not ruled out through a lumbar puncture. The primary outcome was sepsis-related mortality. The secondary outcome was the development of brain lesions. Timing, in vitro activity and appropriateness of empirical antimicrobials, were compared between fatal and non-fatal episodes. Uni- and multi-variable analyses were carried out for the primary outcome. Results: Time to antibiotics and in vitro activity of empirical antimicrobials were similar between fatal and non-fatal cases. By contrast, empirical antimicrobials were appropriate in a lower proportion of fatal episodes of late-onset sepsis (4/17, 24%) compared to non-fatal episodes (39/66, 59%). After adjusting for Gram-negative vs. Gram-positive pathogen and for other supportive measures (time to volume administration), inappropriate empirical antimicrobials remained associated with mortality (aOR, 10.3; 95% CI, 1.4–76.8, p = 0.023), while timing to first antibiotics was not (aOR 0.9; 95% CI, 0.7–1.2, p = 0.408; AUC = 0.88). The association between appropriate antimicrobials and brain sequelae was also significant (p = 0.024). Conclusions: The risk of sepsis-related mortality and brain sequelae in preterm very low birth weight infants is significantly associated with the appropriateness (rather than the timing and the in vitro activity) of empirical antimicrobials. Until meningitis is ruled out through lumbar puncture, septic very low birth weight infants at high risk of mortality should receive empiric antimicrobials with high delivery through the blood-brain barrier

Rescue of secretion of rare-disease associated misfolded mutant glycoproteins in UGGT1 knock-out mammalian cells

Endoplasmic reticulum (ER) retention of misfolded glycoproteins is mediated by the ER-localised eukaryotic glycoprotein secretion checkpoint, UDP-glucose glycoprotein glucosyl-transferase (UGGT). The enzyme recognises a misfolded glycoprotein and flags it for ER retention by re-glucosylating one of its N-linked glycans. In the background of a congenital mutation in a secreted glycoprotein gene, UGGT-mediated ER retention can cause rare disease, even if the mutant glycoprotein retains activity (“responsive mutant”). Using confocal laser scanning microscopy, we investigated here the subcellular localisation of the human Trop-2-Q118E, E227K and L186P mutants, which cause gelatinous drop-like corneal dystrophy (GDLD). Compared with the wild type Trop-2, which is correctly localised at the plasma membrane, these Trop-2 mutants are retained in the ER. We studied fluorescent chimeras of the Trop-2 Q118E, E227K and L186P mutants in mammalian cells harbouring CRISPR/Cas9-mediated inhibition of the UGGT1 and/or UGGT2 genes. The membrane localisation of the Trop-2 Q118E, E227K and L186P mutants was successfully rescued in UGGT1-/- cells. UGGT1 also efficiently reglucosylated Trop-2-Q118E-EYFP in cellula. The study supports the hypothesis that UGGT1 modulation would constitute a novel therapeutic strategy for the treatment of pathological conditions associated to misfolded membrane glycoproteins (whenever the mutation impairs but does not abrogate function), and it encourages the testing of modulators of ER glycoprotein folding quality control as broad-spectrum rescue-of-secretion drugs in rare diseases caused by responsive secreted glycoprotein mutants