Coccidiosis in Barbari and Jamunapari goats at an organized farm in semi-arid tropical region of India

The aim of the present study was to learn more about caprine coccidiosis in semi-arid tropical regions, including transformation techniques for data analysis and variables impacting faecal oocyst count in Barbari and Jamunapari goats. For this investigation, a total of 1422 goat faeces samples from two separate seasons were tested, and 1342 (94.37%) of them were positive for several coccidian species oocysts. A total of 259 animals, including 153 Barbari (77 males and 76 females) and 106 Jamunapari (45 males and 61 females), provided the 777 faecal samples from the autumn season. In contrast, 215 animals, including 110 Barbari (60 males and 50 females) and 105 Jamunapari (52 males and 53 females), provided the 645 faecal samples from the spring season. At 3.5, 6.0, and 7.5 months of age, both breeds had their faeces examined. The geometric mean of oocysts per Gram (OPG) for the Barbari breeds was greater than the Jamunapari breeds at 6.637a±0.03 (662) and 6.521b±0.03 (579), respectively. The Transformed oocysts per gram (LOPG) was also greater in females 6.651a±0.03 (673) than in males. Similar to the age comparison, the 7.5 month older animal showed a greater count of 6.858a±0.04 (851) than the 3.5 and 6.0 month old animals. The five found coccidian oocysts were Eimeria arloingi, Eimeria ninakohlyakimovae, Eimeria hirci, Eimeria caprina, and Eimeria christenseni. The oocysts Eimeria arloingi (39–45%), Eimeria ninakohlyakimovae (25–31%), Eimeria hirci (13-20%), Eimeria christenseni (6–12%), and Eimeria caprina (3–10%) were present in both breeds and seasons of male and females

Ayurveda Practitioners Consensus to Develop Strategies for Prevention and Treatment of Corona Virus Disease (COVID-19)

Background: The outbreak of Coronavirus disease (COVID-19) has been recently declared as Public Health Emergency of International Concern (PHEIC) by World Health Organization and the virus has now spread beyond the boundaries of countries and continents. At present, no specific antiviral treatment or vaccine is available or recommended to counter the COVID-19, and the potential therapy is still symptomatic. Objective: The primary aim of the study is to review ancient classical literatures and past human treatment protocols of Ayurveda for prevention and treatment of infectious diseases and flu like illnesses, so as to provide guidance for the prevention of COVID-19. Methods: Classical Ayurveda and recent modern literature with treatment protocols of Ayurveda for prevention and treatment of flu and infectious diseases were reviewed and COVID-19 has been categorized in four specific situations based on the severity of clinical condition. Total 110 Ayurveda practitioners of different parts of India were participated in the survey. These specific situations were conveyed and described to these participants and their opinions and options were collected through social media platform i.e. WhatsApp or SMS. Results: Recommendation and suggestions received from more than 50% participants were taken into accounts to draft the recommendation for probable preventive and therapeutic regimen for the disease. Conclusion: The results obtained in the study may provide a preventive strategy to the Ayurveda practitioners as well as common people across the globe by increasing the innate immunity of the body to combat COVID-19. However, till today, social distancing is considered as most effective way to stop the spread of COVID-19. Further, pilot study for different group is recommended

Genome-Wide Identification and Characterization of the Brassinazole-resistant (BZR) Gene Family and Its Expression in the Various Developmental Stage and Stress Conditions in Wheat (Triticum aestivum L.)

Brassinosteroids (BRs) play crucial roles in various biological processes, including plant developmental processes and response to diverse biotic and abiotic stresses. However, no information is currently available about this gene family in wheat (Triticum aestivum L.). In the present investigation, we identified the BZR gene family in wheat to understand the evolution and their role in diverse developmental processes and under different stress conditions. In this study, we performed the genome-wide analysis of the BZR gene family in the bread wheat and identified 20 TaBZR genes through a homology search and further characterized them to understand their structure, function, and distribution across various tissues. Phylogenetic analyses lead to the classification of TaBZR genes into five different groups or subfamilies, providing evidence of evolutionary relationship with Arabidopsis thaliana, Zea mays, Glycine max, and Oryza sativa. A gene exon/intron structure analysis showed a distinct evolutionary path and predicted the possible gene duplication events. Further, the physical and biochemical properties, conserved motifs, chromosomal, subcellular localization, and cis-acting regulatory elements were also examined using various computational approaches. In addition, an analysis of public RNA-seq data also shows that TaBZR genes may be involved in diverse developmental processes and stress tolerance mechanisms. Moreover, qRT-PCR results also showed similar expression with slight variation. Collectively, these results suggest that TaBZR genes might play an important role in plant developmental processes and various stress conditions. Therefore, this work provides valuable information for further elucidate the precise role of BZR family members in wheat

Crosstalk between AML and stromal cells triggers acetate secretion through the metabolic rewiring of stromal cells

Acute myeloid leukaemia (AML) cells interact and modulate components of their surrounding microenvironment into their own benefit. Stromal cells have been shown to support AML survival and progression through various mechanisms. Nonetheless, whether AML cells could establish beneficial metabolic interactions with stromal cells is underexplored. By using a combination of human AML cell lines and AML patient samples together with mouse stromal cells and a MLL-AF9 mouse model, here we identify a novel metabolic crosstalk between AML and stromal cells where AML cells prompt stromal cells to secrete acetate for their own consumption to feed the tricarboxylic acid cycle (TCA) and lipid biosynthesis. By performing transcriptome analysis and tracer-based metabolic NMR analysis, we observe that stromal cells present a higher rate of glycolysis when co-cultured with AML cells. We also find that acetate in stromal cells is derived from pyruvate via chemical conversion under the influence of reactive oxygen species (ROS) following ROS transfer from AML to stromal cells via gap junctions. Overall, we present a unique metabolic communication between AML and stromal cells and propose two different molecular targets, ACSS2 and gap junctions, that could potentially be exploited for adjuvant therapy

Pd Nanoparticles and Thin Films for Room Temperature Hydrogen Sensor

We report the application of palladium nanoparticles and thin films for hydrogen sensor. Electrochemically grown palladium particles with spherical shapes deposited on Si substrate and sputter deposited Pd thin films were used to detect hydrogen at room temperature. Grain size dependence of H2sensing behavior has been discussed for both types of Pd films. The electrochemically grown Pd nanoparticles were observed to show better hydrogen sensing response than the sputtered palladium thin films. The demonstration of size dependent room temperature H2sensing paves the ways to fabricate the room temperature metallic and metal–metal oxide semiconductor sensor by tuning the size of metal catalyst in mixed systems. H2sensing by the Pd nanostructures is attributed to the chemical and electronic sensitization mechanisms

Constraints on the χ_(c1) versus χ_(c2) polarizations in proton-proton collisions at √s = 8 TeV

The polarizations of promptly produced χ_(c1) and χ_(c2) mesons are studied using data collected by the CMS experiment at the LHC, in proton-proton collisions at √s=8  TeV. The χ_c states are reconstructed via their radiative decays χ_c → J/ψγ, with the photons being measured through conversions to e⁺e⁻, which allows the two states to be well resolved. The polarizations are measured in the helicity frame, through the analysis of the χ_(c2) to χ_(c1) yield ratio as a function of the polar or azimuthal angle of the positive muon emitted in the J/ψ → μ⁺μ⁻ decay, in three bins of J/ψ transverse momentum. While no differences are seen between the two states in terms of azimuthal decay angle distributions, they are observed to have significantly different polar anisotropies. The measurement favors a scenario where at least one of the two states is strongly polarized along the helicity quantization axis, in agreement with nonrelativistic quantum chromodynamics predictions. This is the first measurement of significantly polarized quarkonia produced at high transverse momentum