Perturbation Analysis of Calcium, Alkalinity and Secretion during Growth of Lily Pollen Tubes

Pollen tubes grow by spatially and temporally regulated expansion of new material secreted into the cell wall at the tip of the tube. A complex web of interactions among cellular components, ions and small molecule provides dynamic control of localized expansion and secretion. Cross-correlation studies on oscillating lily (Lilium formosanum Wallace) pollen tubes showed that an increase in intracellular calcium follows an increase in growth, whereas the increase in the alkaline band and in secretion both anticipate the increase in growth rate. Calcium, as a follower, is unlikely to be a stimulator of growth, whereas the alkaline band, as a leader, may be an activator. To gain further insight herein we reversibly inhibited growth with potassium cyanide (KCN) and followed the re-establishment of calcium, pH and secretion patterns as growth resumed. While KCN markedly slows growth and causes the associated gradients of calcium and pH to sharply decline, its removal allows growth and vital processes to fully recover. The calcium gradient reappears before growth restarts; however, it is preceded by both the alkaline band and secretion, in which the alkaline band is slightly advanced over secretion. Thus the pH gradient, rather than the tip-focused calcium gradient, may regulate pollen tube growth

Bullying Experiences of Individuals with Visual Impairment: The Mitigating Role of Sport Participation

In bullying research, status plays a key role in who is the instigator and who is the recipient of bullying. Athletes are often considered a high status individual and have been accused of engaging in bullying behaviors. Individuals with disabilities are seen as possessing lower status and are often the victims of bullying. What is unclear is if athletes who have a disability are instigators of bullying or are victims themselves. This study explored implications of status as it relates to bullying experiences by athletes and non-athletes with visual impairments. Specific attention was paid to uncovering similarities and differences between the two groups. Results indicate that individuals with visual impairments are both the victim and instigator of bullying activities. The hypothesis of status as a factor in bullying was supported within both populations

Trapping Phosphorus in Runoff with a Phosphorus Removal Structure

Reduction of phosphorus (P) inputs to surface waters may decrease eutrophication. Some researchers have proposed fi ltering dissolved P in runoff with P-sorptive byproducts in structures placed in hydrologically active areas with high soil P concentrations. Th e objectives of this study were to construct and monitor a P removal structure in a suburban watershed and test the ability of empirically developed fl ow-through equations to predict structure performance. Steel slag was used as the P sorption material in the P removal structure. Water samples were collected before and after the structure using automatic samples and analyzed for total dissolved P. During the fi rst 5 mo of structure operation, 25% of all dissolved P was removed from rainfall and irrigation events. Phosphorus was removed more effi ciently during low fl ow rate irrigation events with a high retention time than during high fl ow rate rainfall events with a low retention time. Th e six largest fl ow events occurred during storm fl ow and accounted for 75% of the P entering the structure and 54% of the P removed by the structure. Flow-through equations developed for predicting structure performance produced reasonable estimates of structure “lifetime” (16.8 mo). However, the equations overpredicted cumulative P removal. Th is was likely due to diff erences in pH, total Ca and Fe, and alkalinity between the slag used in the structure and the slag used for model development. Th is suggests the need for an overall model that can predict structure performance based on individual material properties

Trapping Phosphorus in Runoff with a Phosphorus Removal Structure

Reduction of phosphorus (P) inputs to surface waters may decrease eutrophication. Some researchers have proposed fi ltering dissolved P in runoff with P-sorptive byproducts in structures placed in hydrologically active areas with high soil P concentrations. Th e objectives of this study were to construct and monitor a P removal structure in a suburban watershed and test the ability of empirically developed fl ow-through equations to predict structure performance. Steel slag was used as the P sorption material in the P removal structure. Water samples were collected before and after the structure using automatic samples and analyzed for total dissolved P. During the fi rst 5 mo of structure operation, 25% of all dissolved P was removed from rainfall and irrigation events. Phosphorus was removed more effi ciently during low fl ow rate irrigation events with a high retention time than during high fl ow rate rainfall events with a low retention time. Th e six largest fl ow events occurred during storm fl ow and accounted for 75% of the P entering the structure and 54% of the P removed by the structure. Flow-through equations developed for predicting structure performance produced reasonable estimates of structure “lifetime” (16.8 mo). However, the equations overpredicted cumulative P removal. Th is was likely due to diff erences in pH, total Ca and Fe, and alkalinity between the slag used in the structure and the slag used for model development. Th is suggests the need for an overall model that can predict structure performance based on individual material properties

atToc159 is a selective transit peptide receptor for the import of nucleus-encoded chloroplast proteins

The members of the Toc159 family of GTPases act as the primary receptors for the import of nucleus-encoded preproteins into plastids. Toc159, the most abundant member of this family in chloroplasts, is required for chloroplast biogenesis (Bauer, J., K. Chen, A. Hiltbunner, E. Wehrli, M. Eugster, D. Schnell, and F. Kessler. 2000. Nature. 403:203–207) and has been shown to covalently cross-link to bound preproteins at the chloroplast surface (Ma, Y., A. Kouranov, S. LaSala, and D.J. Schnell. 1996. J. Cell Biol. 134:1–13; Perry, S.E., and K. Keegstra. 1994. Plant Cell. 6:93–105). These reports led to the hypothesis that Toc159 functions as a selective import receptor for preproteins that are required for chloroplast development. In this report, we provide evidence that Toc159 is required for the import of several highly expressed photosynthetic preproteins in vivo. Furthermore, we demonstrate that the cytoplasmic and recombinant forms of soluble Toc159 bind directly and selectively to the transit peptides of these representative photosynthetic preproteins, but not representative constitutively expressed plastid preproteins. These data support the function of Toc159 as a selective import receptor for the targeting of a set of preproteins required for chloroplast biogenesis

A computational group theoretic symmetry reduction package for the SPIN model checker

Symmetry reduced model checking is hindered by two problems: how to identify state space symmetry when systems are not fully symmetric, and how to determine equivalence of states during search. We present TopSpin, a fully automatic symmetry reduction package for the Spin model checker. TopSpin uses the Gap computational algebra system to effectively detect state space symmetry from the associated Promela specification, and to choose an efficient symmetry reduction strategy by classifying automorphism groups as a disjoint/wreath product of subgroups. We present encouraging experimental results for a variety of Promela examples

Intraoperative detection of insufficient surgical margins in head and neck cancer resection using dual aperture fluorescence ratio imaging

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Direct Molecular Detection and Genotyping of Borrelia burgdorferi from Whole Blood of Patients with Early Lyme Disease

Direct molecular tests in blood for early Lyme disease can be insensitive due to low amount of circulating Borrelia burgdorferi DNA. To address this challenge, we have developed a sensitive strategy to both detect and genotype B. burgdorferi directly from whole blood collected during the initial patient visit. This strategy improved sensitivity by employing 1.25 mL of whole blood, a novel pre-enrichment of the entire specimen extract for Borrelia DNA prior to a multi-locus PCR and electrospray ionization mass spectrometry detection assay. We evaluated the assay on blood collected at the initial presentation from 21 endemic area patients who had both physician-diagnosed erythema migrans (EM) and positive two-tiered serology either at the initial visit or at a follow-up visit after three weeks of antibiotic therapy. Results of this DNA analysis showed detection of B. burgdorferi in 13 of 21 patients (62%). In most cases the new assay also provided the B. burgdorferi genotype. The combined results of our direct detection assay with initial physician visit serology resulted in the detection of early Lyme disease in 19 of 21 (90%) of patients at the initial visit. In 5 of 21 cases we demonstrate the ability to detect B. burgdorferi in early Lyme disease directly from whole blood specimens prior to seroconversion