Dry fractionation of plant material powders using an electrostatic corona separator: a model study

In a dry bioreffinery scheme, the separation of plant materials into fractions of interest compound is a crucial step. In recent years, electrostatic separation of agri-resources has sparked a growing interest for its potential but optimization efforts remain to be done especially in case of fine powders. The present work is an experimental investigation of factors, which influence the separation process of plant biomass powders in a custom built corona electrostatic separator with view to optimization. Three particle size classes of cork, semolina and wood powders have been characterized in term of charge decay curves and their behavior on the device have been studied. Separation tests of a blend constituted of 1g of wood powder and 1g of semolina have also been conducted with very promising results. Please click Additional Files below to see the full abstract

Fractionnement par voie sèche de la biomasse ligno-cellulosique (broyage poussé de la paille de blé et effets sur ses bioconversions)

Dans le contexte de la bioraffinerie végétale pour la production de molécules et d'énergie, des prétraitements sont nécessaires pour augmenter la réactivité de la biomasse ligno-cellulosique. Cette thèse s'insère dans une thématique dont l'objectif général est d'établir les bases d'une raffinerie du végétal par des procédés par voie sèche. Cette étude a pour objectif de développer et comprendre le fractionnement mécanique poussé de la paille de blé jusqu'à des tailles sub-millimétriques et d'évaluer les effets sur des procédés de bioconversions énergétiques. La paille de blé présente une grande hétérogénéité à plusieurs niveaux d'échelle (du cm au m). Un diagramme de broyage multi-étapes à l'échelle pilote (>1kg) a permis d'obtenir une large gamme de tailles de particules, par 3 modes de sollicitation distincts: i) broyages à grille sélective produisant des tailles du grossier (800 m) au fin (50 m), ii) broyage à jet d'air (ultrafin, ~20 m) et iii) broyage à boulets (ultrafin, ~10 m). Une méthodologie d'analyse morphologique des particules a été développée par analyse d'images de microscopie optique. La paille soumise aux mécanismes complexes de rupture lors de broyages produit une forte variabilité des formes et compositions des particules. L'analyse multiple de co-inertie a permis d'évaluer de façon globale les morphologies des particules. Globalement, le broyage diminue la taille et les facteurs de forme des particules, avec quelques exceptions dues aux configurations matérielles. La dégradabilité enzymatique (saccharification) des poudres produites a été améliorée par la réduction de la taille des particules. Jusqu'à ~100 m la solubilisation des polysaccharides augmente puis se stabilise à 36 % des polysaccharides totaux et 40 % de la cellulose. Seuls les échantillons issus du broyage à boulets dépassent cette limite et atteignent 46 % d'hydrolyse des polysaccharides totaux et 72 % de la cellulose. Ceci est lié à une augmentation de l'efficacité enzymatique due à la diminution de la cristallinité de la cellulose (de 22 à 13%). Ces résultats du broyage à boulets sont comparables à ceux de l'explosion à la vapeur, avec une meilleure préservation des hémicelluloses. Cette amélioration d'efficacité enzymatique s'est traduite par des dégradations anaérobies (biogaz) accélérées et légèrement augmentées (cas du broyeur à boulets). La décomposition aérobie dans le sol a été améliorée par le broyage grossier, mais les broyages plus fins n'ont pas entrainé de gain. Les caractéristiques de la paille broyée peuvent varier selon l'intensité et le mode de broyage. Bien que tous les broyages permettent la réduction de la taille, le broyage à grilles et le broyage à jet d'air n'engendrent pas de changements dans la structure fine des polymères pariétaux. Seul le broyage à boulets a engendré des changements de la structure interne des particules notamment en réduisant la cristallinité de la cellulose et en solubilisant partiellement les hémicelluloses. Ces résultats permettent de mettre en évidence que la fragmentation mécanique poussée par voie sèche est une alternative possible aux prétraitements utilisés en raffinerie végétale.In a context of plant biorefinery for the production of molecules and energy, pretreatments are necessary to increase the reactivity of the lignocellulosic biomass. This thesis is part of a general project aiming to establish the bases for a dry plant refinery. This study aimed to develop and understand advanced mechanical fractionation of wheat straw down to sub-millimeter sizes and to assess its effects on bioconversion processes for bioenergy. Wheat straw exhibited a high heterogeneity at several scale levels (from cm to m). A multistep diagram of dry grinding at pilot-scale (> 1 kg) produced a wide range of particle sizes by three distinct mode of action: i) sieve-based grinding producing particle sizes from coarse (800 m) to fine (50 m), ii) air-jet milling (ultra-fine, ~ 20 m) and iii) ball milling (ultra-fine, ~ 10 "m). A morphological analysis of particles was developed by image analysis from light microscopy. Subjecting wheat straw to the complex breaking mechanisms during grinding produced particles highly variable in shapes and compositions. A multiple co-inertia analysis allowed the evaluation of the overall particle morphologies. Generally, grinding reduced the size and shape descriptors of particles, with some exceptions due to equipment configurations. The enzymatic degradability (saccharification) of produced powders was improved by reducing their particle size. Until ~ 100 m the polysaccharides solubilisation was increased and then stabilised at 36% total polysaccharides and 40% cellulose. Only samples from ball milling overcome this limit and attained hydrolysis yields of 46% total polysaccharides and 72% cellulose. This is due to an increase in enzymatic efficiency by the reduction of cellulose crystallinity (from 22 to 13%). These results of ball milling are comparable to those of steam explosion process, with a better preservation of hemicelluloses. This improved enzymatic efficiency resulted in faster and slightly more extensive (ball milling case) anaerobic degradations (biogas). Aerobic decomposition in the soil was improved by coarse grinding, but finer grinding did not result in a further increase. The characteristics of ground straw varied depending on grinding intensity and mode. Although all grindings could reduce the size, sieve-based grinding and air-jet milling did not allow changes in the fine structure of cell wall polymers. Only ball milling led to changethe internal structure of particles especially reducing cellulose crystallinity and partially solubilising hemicelluloses. These results demonstrate that advanced mechanical fragmentation by dry processes is a possible alternative for pretreatments in a plant refineryMONTPELLIER-SupAgro La Gaillarde (341722306) / SudocSudocFranceF

Automated assay for screening the enzymatic release of reducing sugars from micronized biomass

<p>Abstract</p> <p>Background</p> <p>To reduce the production cost of bioethanol obtained from fermentation of the sugars provided by degradation of lignocellulosic biomass (<it>i.e</it>., second generation bioethanol), it is necessary to screen for new enzymes endowed with more efficient biomass degrading properties. This demands the set-up of high-throughput screening methods. Several methods have been devised all using microplates in the industrial SBS format. Although this size reduction and standardization has greatly improved the screening process, the published methods comprise one or more manual steps that seriously decrease throughput. Therefore, we worked to devise a screening method devoid of any manual steps.</p> <p>Results</p> <p>We describe a fully automated assay for measuring the amount of reducing sugars released by biomass-degrading enzymes from wheat-straw and spruce. The method comprises two independent and automated steps. The first step is the making of "substrate plates". It consists of filling 96-well microplates with slurry suspensions of micronized substrate which are then stored frozen until use. The second step is an enzymatic activity assay. After thawing, the substrate plates are supplemented by the robot with cell-wall degrading enzymes where necessary, and the whole process from addition of enzymes to quantification of released sugars is autonomously performed by the robot. We describe how critical parameters (amount of substrate, amount of enzyme, incubation duration and temperature) were selected to fit with our specific use. The ability of this automated small-scale assay to discriminate among different enzymatic activities was validated using a set of commercial enzymes.</p> <p>Conclusions</p> <p>Using an automatic microplate sealer solved three main problems generally encountered during the set-up of methods for measuring the sugar-releasing activity of plant cell wall-degrading enzymes: throughput, automation, and evaporation losses. In its present set-up, the robot can autonomously process 120 triplicate wheat-straw samples per day. This throughput can be doubled if the incubation time is reduced from 24 h to 4 h (for initial rates measurements, for instance). This method can potentially be used with any insoluble substrate that is micronizable. A video illustrating the method can be seen at the following URL: <url>http://www.youtube.com/watch?v=NFg6TxjuMWU</url></p

Production, purification et caracterisation de polyosidases du basidiomycete Dichomitus squalens

SIGLECNRS T 58517 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc

Method for the fractionation of an oilseed cake and applications of said method

The invention relates to a dry method (40) for the fractionation of oilseed cakes, said method comprising: a step (410) of grinding oilseed cakes into a powder of ultrafine particles; a step (420) of triboelectrostatically charging ultrafine particles of said powder; and at least oneLe procédé (40) de fractionnement de tourteaux d'oléagineux par voie sèche comporte : - une étape (410) de broyage de tourteaux d'oléagineux en une poudre de particules ultrafines, - une étape (420) de charge tribo-électrostatique de particules ultrafines de ladite poudre et - au moins une étape (425) de déviation de trajectoire dans le champ électrique des particules chargées pour trier les particules et fournir au moins une fraction enrichie en ligno-cellulose

FTIR and Raman signatures of wheat grain peripheral tissues

Corresponding author: [email protected] audienceHand-dissection of mature grains from three common wheat cultivars (Triticum aestivum L.: Caphorn, Crousty, and Recital) and one durum wheat (T durum Desf., Ardente) was performed to obtain pure samples of aleurone layer. hyaline layer, outer pericarp, and a composite layer made up of testa+inner pericarp. Vibrational spectral signatures were collected on both sides of the layers by ATR-FTIR and Raman spectroscopy. Spectra were compared with biochemical analysis on the same samples which allowed identification of specific composition patterns in each tissue regardless of the cultivar. Considering the low penetration depth of ATR-FTIR signal, the cuticles were evidenced on the external sides of outer pericarp, hyaline layer, and testa. Spectra from testa of red and white wheats were clearly distinguished. FTIR spectroscopy, combined with statistical analysis, was successful in identifying the specific spectral signature for each peripheral tissue of wheat grains. In the 1500-800 cm(-1) spectral region, multivariate models allowed accurate prediction of the histological origin of the pericarp, hyaline, and aleurone layers regardless of the analyzed side, and the testa but with a lower performanc

New dry technology of environmentally friendly biomass refinery: glucose yield and energy efficiency.

BACKGROUND: Today, most of pretreatments used to convert biomass into biofuels are based on expensive chemical processes that not only do not keep the major components intact after separation, but also consume water and generate many effluents. However, dry fractionation technologies are an important step for future biomass biorefineries since they do not require chemicals and do not generate wastewater. Therefore, the aim of the present study was to evaluate the feasibility of using milling combined with an electrostatic fractionation (ES) of wheat straw (WS) as a way to separate fractions that are enriched in cellulose and more enzymatically accessible, from recalcitrant tissues enriched in lignin-hemicelluloses, in order to produce biofuels.[br/]RESULTS: After milling, WS particles are introduced into a tribo-electrostatic separator, where they are positively or negatively charged by tribo-electricity. Then they are introduced into a separation cell comprising two electrodes (+ and -). The negative electrode attracts the positively charged particles and the positive electrode attracts the negatively charged particles. Results show that amorphous cellulose rich particles were clearly more abundant in positively charged fractions (F+), and loose crystalline cellulose, lignin-xylan and ash-containing material were more abundant in negatively charged fractions (F-). Indeed, positively charged fractions (F+) are more accessible upon enzymatic hydrolysis, which resulted, for example, in sugars yield of 43.5% glucose (254 gKg(-1)) for F2B + compared to 25.2% (103 gKg(-1)) for F2A-, and 26.3% (130 gKg(-1)) for unfractionated WS F0, respectively.[br/]CONCLUSION: The combination strategy of milling and ES fractionation could improve the economic feasibility by low energy consumption (10.5 WhKg(-1)) and it produces reactive lignocelluloses particles with different physicochemical structures, which can be converted easily into biofuels and biomaterials without generating toxic effluents