Qual a influência do oxigênio em alta concentração na câmara hiperbárica no metabolismo ósseo?

Objectives: To know the actions of oxygen in high concentration in the hyperbaric chamber (HC) in the expression of genes related to bone metabolism in osteoblastic cell lines and human trabecular bone. Material and methods: The differential expression of several genes related to bone metabolism (SOST, RUNX2, MMP14, OPG, HIF-1α and SIRT1) was analyzed in two cell lines of human osteoblasts (Saos and Super-Saos) and in bone fragments human trabecular submitted to one, three or five sessions of CH (90 minutes, 100% oxygen; 2.3 atmospheres). A control that did not receive CH was used in each experiment. Results: We found no significant differences after CH in the expression of the studied genes, neither in cells nor in trabecular bone. Only in the Super-Saos strain did OPG expression decrease after 5 CH sessions 6 times compared to the control group (2 -ΔCt of 72; p = 0.01). Conclusions: Oxygen in high concentrations in the hyperbaric chamber does not appear to influence the expression of genes related to bone metabolism.Objetivos: Conhecer as ações do oxigênio em alta concentração na câmara hiperbárica (HC) na expressão de genes relacionados ao metabolismo ósseo em linhagens de células osteoblásticas e osso trabecular humano. Material e métodos: A expressão diferencial de vários genes relacionados ao metabolismo ósseo (SOST, RUNX2, MMP14, OPG, HIF-1α e SIRT1) foi analisada em duas linhagens celulares de osteoblastos humanos (Saos e Super-Saos) e em fragmentos de osso trabecular humano submetidos a uma, três ou cinco sessões de CH (90 minutos, oxigênio a 100%; 2,3 atmosferas). Um controle que não recebeu CH foi usado em cada experimento. Resultados: Não encontramos diferenças significativas após o CH na expressão dos genes estudados, nem nas células nem no osso trabecular. Apenas na linhagem Super-Saos a expressão de OPG após 5 sessões de CH diminuiu 6 vezes em relação ao grupo controle (2 -ΔCt de 72; p = 0,01). Conclusões: O oxigênio em altas concentrações na câmara hiperbárica não parece influenciar a expressão de genes relacionados ao metabolismo ósseo

Processo para produção de microcápsulas dotadas de propriedades magnéticas, produto obtido e método para a liberação controlada de substâncias ativas

Em 07/10/2015: Anuidade de pedido de patente de invenção no prazo ordinário.DepositadaA presente invenção descreve um processo de produção de microcápsulas contendo nanopartículas magnéticas para liberação controlada de substâncias ativas, bem como produtos relacionados a esse processo e métodos de liberação controlada de substâncias ativas a partir da aplicação de campo magnético oscilante. Em uma concretização preferencial, a síntese das nanopartículas é realizada in situ, na presença de alginato e quitosana, incorporando as propriedades magnéticas às microcápsulas. A liberação controlada de substâncias ativas como, por exemplo, a insulina, se dá por aplicação de campo magnético oscilante

Presbyvestibulopathy, Comorbidities, and Perception of Disability: A Cross-Sectional Study

Objective: To assess the perception of disability in patients with presbyvestibulopathy and to determine the factors (demographic, balance test scores, and comorbidities) that determine higher levels of disability. Material and Methods: This was a cross-sectional study conducted in a tertiary university hospital. There were 103 patients who fulfilled the diagnostic criteria for presbyvestibulopathy and were included. Dizziness Handicap Inventory (DHI) score was the main variable used to quantify disability. Influence on DHI score, sex, age, time of evolution, equilibriometric parameters (posturographic scores and timed up and go test), history of falls, comorbidities (high blood pressure, diabetes, and dyslipidemia), psychotropic drug use, tobacco or alcohol use, living environment (urban or rural), and active lifestyle were analyzed. Results: Most of the DHI scores showed a moderate (46 patients, 44.7%) or severe (39 participants, 37.9%) handicap. DHI scores were higher in women (59.8 vs. 36.1, p < 0.001), patients with obesity (58.92 vs. 48.68; p = 0.019), benzodiazepine (59.9 vs. 49.1, p = 0.008) or other psychotropic drug (60.7 vs. 49.2, p = 0.017) users, and fallers (57.1 vs. 47.3, p = 0.048). There was also a significant positive correlation between DHI score, time (Rho coefficient: 0.371, p < 0.001), and steps (Rho coefficient: 0.284, p = 0.004) used in the TUG and with the short FES-I questionnaire (a shortened version of the Falls Efficacy Scale-International) score (Rho coefficient: 0.695, p < 0.001). DHI scores were lower in alcohol consumers than in non-drinkers (46.6 vs. 56, p = 0.048). No significant correlation was found between DHI scores and age, time of evolution, posturographic scores, comorbidities, environment (rural or urban), or active lifestyle. Conclusion: Most patients with presbyvestibulopathy show an important subjective perception of disability in relation to their symptoms. This perception is substantially higher in women than in men. The most influential factors are difficulties in walking, fear of falling, and obesity. Unique Identifier: NCT03034655, www.clinicaltrials.gov

Modified Timed Up and Go Test for Tendency to Fall and Balance Assessment in Elderly Patients With Gait Instability

Objective: To compare the results from the modified Timed Up and Go Test (TUG) with posturographic variables, the subjective perception of disability due to gait instability, and the number of falls in a sample of the elderly population with imbalance, to confirm that the TUG Test is a useful clinical instrument to assess the tendency to fall in individuals of this age group. Materials and Methods: Cross-sectional study conducted in a tertiary university hospital, in 174 people aged 65 years or older with gait instability. Modified TUG Test was performed; time, step count and the need for support during the test were the analyzed variables. They were compared with the number of falls, Computerized Dynamic Posturography scores, and questionnaires scores (Dizziness Handicap Inventory and a shortened version of the Falls Efficacy Scale-International). Results: The average time to complete the TUG Test was 21.24 +/- 8.18 s, and the average step count was 27.36 +/- 7.93. One hundred two patients (58.6%) required no support to complete the test, whereas the other 72 (41.4%) used supports. The time taken to complete the Test was significantly related with having or not having fallen in the previous year, with the scores of the questionnaires, and with various parameters of dynamic posturography. A higher percentage of patients who took more than 15 s had fallen in the previous year than those who took up to 15 s to complete the test [P = 0.012; OR = 2.378; 95% CI (1.183, 4.780)]. No significant correlation was found between the step count and the number of falls in the previous year, with falling during the test or not, or with being a single or a frequent faller. No relation was found between the need for supports and the number of falls, with having or not having fallen in the previous year, or with being a single or frequent faller. Conclusion: The modified TUG Test is in relation with the presence or absence of falls. Time is the essential parameter for analyzing the risk of falling and the 15-s threshold is a good value to differentiate elderly patients at high risk of falling. Unique Identifier: NCT03034655, www.clinicaltrials.gov

Fósforo em cronossequência de cana-de-açúcar queimada no cerrado goiano: análise de ácidos húmicos por RMN de 31P

The aim of this study was to identify, with the use of 31P NMR spectroscopy, organic P species in humic acids (HA) in samples from Oxisol cultivated in chronosequence with sugar cane, pasture and Cerrado. The main forms of P-type found were orthophosphate, monoester-P (phosphate sugars) and P-diester (orthophosphate). The 31P NMR technique proved capable of identifying changes in the areas studied as a function of sugar cane burning time. In areas with 1 and 5 years of burnt cane, a decrease in recalcitrant organic P in humic acids indicated the need for use of P-humic substances for plant nutrition