242 research outputs found
A Fuzzy Inference System for the Assessment of Indoor Air Quality in an Operating Room to Prevent Surgical Site Infection
Indoor air quality in hospital operating rooms is of great concern for the prevention of surgical site infections (SSI). A wide range of relevant medical and engineering literature has shown that the reduction in air contamination can be achieved by introducing a more efficient set of controls of HVAC systems and exploiting alarms and monitoring systems that allow having a clear report of the internal air status level. In this paper, an operating room air quality monitoring system based on a fuzzy decision support system has been proposed in order to help hospital staff responsible to guarantee a safe environment. The goal of the work is to reduce the airborne contamination in order to optimize the surgical environment, thus preventing the occurrence of SSI and reducing the related mortality rate. The advantage of FIS is that the evaluation of the air quality is based on easy-to-find input data established on the best combination of parameters and level of alert. Compared to other literature works, the proposed approach based on the FIS has been designed to take into account also the movement of clinicians in the operating room in order to monitor unauthorized paths. The test of the proposed strategy has been executed by exploiting data collected by ad-hoc sensors placed inside a real operating block during the experimental activities of the “Bacterial Infections Post Surgery” Project (BIPS). Results show that the system is capable to return risk values with extreme precision
Neuroprotective potential of isothiocyanates in an in vitro model of neuroinflammation
Isothiocyanates (ITCs), present as glucosinolate precursors in cruciferous vegetables, have shown anti-inflammatory, antioxidant
and anticarcinogenic activities. Here, we compared the effects of three different ITCs on ROS production and on
the expression of matrix metalloproteinase (MMP)-2 and -9, which represent important pathogenetic factors of various
neurological diseases. Primary cultures of rat astrocytes were activated by LPS and simultaneously treated with different
doses of Allyl isothiocyanate (AITC), 2-Phenethyl isothiocyanate (PEITC) and 2-Sulforaphane (SFN). Results showed
that SFN and PEITC were able to counteract ROS production induced by H2O2.
The zymographic analysis of cell culture
supernatants evidenced that PEITC and SFN were the most effective inhibitors of MMP-9, whereas, only SFN significantly
inhibited MMP-2 activity. PCR analysis showed that all the ITCs used significantly inhibited both MMP-2 and MMP-9
expression. The investigation on the mitogen-activated protein kinase (MAPK) signaling pathway demonstrated that ITCs
modulate MMP transcription by inhibition of extracellular-regulated protein kinase (ERK) activity. Results of this study
suggest that ITCs could be promising nutraceutical agents for the prevention and complementary treatment of neurological
diseases associated with MMP involvement
Neuroprotective potential of isothiocyanates in an in vitro model of neuroinflammation
Isothiocyanates (ITCs), present as glucosinolate precursors in cruciferous vegetables, have shown anti-inflammatory, antioxidant and anticarcinogenic activities. Here, we compared the effects of three different ITCs on ROS production and on the expression of matrix metalloproteinase (MMP)-2 and -9, which represent important pathogenetic factors of various neurological diseases. Primary cultures of rat astrocytes were activated by LPS and simultaneously treated with different doses of Allyl isothiocyanate (AITC), 2-Phenethyl isothiocyanate (PEITC) and 2-Sulforaphane (SFN). Results showed that SFN and PEITC were able to counteract ROS production induced by H2O2. The zymographic analysis of cell culture supernatants evidenced that PEITC and SFN were the most effective inhibitors of MMP-9, whereas, only SFN significantly inhibited MMP-2 activity. PCR analysis showed that all the ITCs used significantly inhibited both MMP-2 and MMP-9 expression. The investigation on the mitogen-activated protein kinase (MAPK) signaling pathway demonstrated that ITCs modulate MMP transcription by inhibition of extracellular-regulated protein kinase (ERK) activity. Results of this study suggest that ITCs could be promising nutraceutical agents for the prevention and complementary treatment of neurological diseases associated with MMP involvement
Multifractal Scaling, Geometrical Diversity, and Hierarchical Structure in the Cool Interstellar Medium
Multifractal scaling (MFS) refers to structures that can be described as a
collection of interwoven fractal subsets which exhibit power-law spatial
scaling behavior with a range of scaling exponents (concentration, or
singularity, strengths) and dimensions. The existence of MFS implies an
underlying multiplicative (or hierarchical, or cascade) process. Panoramic
column density images of several nearby star- forming cloud complexes,
constructed from IRAS data and justified in an appendix, are shown to exhibit
such multifractal scaling, which we interpret as indirect but quantitative
evidence for nested hierarchical structure. The relation between the dimensions
of the subsets and their concentration strengths (the "multifractal spectrum'')
appears to satisfactorily order the observed regions in terms of the mixture of
geometries present: strong point-like concentrations, line- like filaments or
fronts, and space-filling diffuse structures. This multifractal spectrum is a
global property of the regions studied, and does not rely on any operational
definition of "clouds.'' The range of forms of the multifractal spectrum among
the regions studied implies that the column density structures do not form a
universality class, in contrast to indications for velocity and passive scalar
fields in incompressible turbulence, providing another indication that the
physics of highly compressible interstellar gas dynamics differs fundamentally
from incompressible turbulence. (Abstract truncated)Comment: 27 pages, (LaTeX), 13 figures, 1 table, submitted to Astrophysical
Journa
Interplay between curvature and Planck-scale effects in astrophysics and cosmology
Several recent studies have considered the implications for astrophysics and
cosmology of some possible nonclassical properties of spacetime at the Planck
scale. The new effects, such as a Planck-scale-modified energy-momentum
(dispersion) relation, are often inferred from the analysis of some quantum
versions of Minkowski spacetime, and therefore the relevant estimates depend
heavily on the assumption that there could not be significant interplay between
Planck-scale and curvature effects. We here scrutinize this assumption, using
as guidance a quantum version of de Sitter spacetime with known Inonu-Wigner
contraction to a quantum Minkowski spacetime. And we show that, contrary to
common (but unsupported) beliefs, the interplay between Planck-scale and
curvature effects can be significant. Within our illustrative example, in the
Minkowski limit the quantum-geometry deformation parameter is indeed given by
the Planck scale, while in the de Sitter picture the parameter of quantization
of geometry depends both on the Planck scale and the curvature scalar. For the
much-studied case of Planck-scale effects that intervene in the observation of
gamma-ray bursts we can estimate the implications of "quantum spacetime
curvature" within robust simplifying assumptions. For cosmology at the present
stage of the development of the relevant mathematics one cannot go beyond
semiheuristic reasoning, and we here propose a candidate approximate
description of a quantum FRW geometry, obtained by patching together pieces
(with different spacetime curvature) of our quantum de Sitter. This
semiheuristic picture, in spite of its limitations, provides rather robust
evidence that in the early Universe the interplay between Planck-scale and
curvature effects could have been particularly significant.Comment: 26 pages
Product Differentiation Costs and Global Competition
The growing competitive intensity on the markets determines the emergence of
competition costs that are expressed at a corporate level and have implicit
repercussions for the supply system. This type of costs makes it possible to identify
a close link between competition costs and supply differentiation costs.
Classification by competitive intensity presupposes that the analysis performed
identifies the classification of company costs as the discriminating element, in
terms of the competitive pressure of the context in which the firm operates.
The emergence of competition costs is linked to an attempt to squeeze them as an
aspect of vertical, or more specifically, horizontal cooperation strategies.Product Differentiation; Differentiation Costs; Over-Supply; Global Competition; Marketing; Market-Driven Management; Global Corporations; Global Markets DOI:http://dx.doi.org/10.4468/2005.1.06garbelli
Immunologic risk stratification of pediatric heart transplant patients by combining HLA-EMMA and PIRCHE-II
Human leukocyte antigen (HLA) molecular mismatch is a powerful biomarker of rejection. Few studies have explored its use in assessing rejection risk in heart transplant recipients. We tested the hypothesis that a combination of HLA Epitope Mismatch Algorithm (HLA-EMMA) and Predicted Indirectly Recognizable HLA Epitopes (PIRCHE-II) algorithms can improve risk stratification of pediatric heart transplant recipients. Class I and II HLA genotyping were performed by next-generation sequencing on 274 recipient/donor pairs enrolled in the Clinical Trials in Organ Transplantation in Children (CTOTC). Using high-resolution genotypes, we performed HLA molecular mismatch analysis with HLA-EMMA and PIRCHE-II, and correlated these findings with clinical outcomes. Patients without pre-formed donor specific antibody (DSA) (n=100) were used for correlations with post-transplant DSA and antibody mediated rejection (ABMR). Risk cut-offs were determined for DSA and ABMR using both algorithms. HLA-EMMA cut-offs alone predict the risk of DSA and ABMR; however, if used in combination with PIRCHE-II, the population could be further stratified into low-, intermediate-, and high-risk groups. The combination of HLA-EMMA and PIRCHE-II enables more granular immunological risk stratification. Intermediate-risk cases, like low-risk cases, are at a lower risk of DSA and ABMR. This new way of risk evaluation may facilitate individualized immunosuppression and surveillance.</p
Immunologic risk stratification of pediatric heart transplant patients by combining HLA-EMMA and PIRCHE-II
Human leukocyte antigen (HLA) molecular mismatch is a powerful biomarker of rejection. Few studies have explored its use in assessing rejection risk in heart transplant recipients. We tested the hypothesis that a combination of HLA Epitope Mismatch Algorithm (HLA-EMMA) and Predicted Indirectly Recognizable HLA Epitopes (PIRCHE-II) algorithms can improve risk stratification of pediatric heart transplant recipients. Class I and II HLA genotyping were performed by next-generation sequencing on 274 recipient/donor pairs enrolled in the Clinical Trials in Organ Transplantation in Children (CTOTC). Using high-resolution genotypes, we performed HLA molecular mismatch analysis with HLA-EMMA and PIRCHE-II, and correlated these findings with clinical outcomes. Patients without pre-formed donor specific antibody (DSA) (n=100) were used for correlations with post-transplant DSA and antibody mediated rejection (ABMR). Risk cut-offs were determined for DSA and ABMR using both algorithms. HLA-EMMA cut-offs alone predict the risk of DSA and ABMR; however, if used in combination with PIRCHE-II, the population could be further stratified into low-, intermediate-, and high-risk groups. The combination of HLA-EMMA and PIRCHE-II enables more granular immunological risk stratification. Intermediate-risk cases, like low-risk cases, are at a lower risk of DSA and ABMR. This new way of risk evaluation may facilitate individualized immunosuppression and surveillance.</p
Taxonomic and functional responses of benthic macroinvertebrate communities to hydrological and water quality variations in a heavily regulated river
Aquatic macroinvertebrates are frequently used to evaluate river system conditions and restoration project performance. A better understanding of macroinvertebrate community responses to multiple stressors is a primary challenge for river science. In this paper, macroinvertebrate responses to hydrological and water quality variability were studied in the regulated Oglio River (northern Italy). We hypothesized that in regulated rivers the hydrological, rather than the physico-chemical conditions, would affect macroinvertebrate communities and biomonitoring tools (taxonomic metrics and functional indices). Repeated sampling (six times a year) was performed at four sites downstream of four dams in a 30 km river stretch during 2014 and 2015. Data were analysed using a linear mixed effect framework, to take into account random variation due to site and sampling date, and with multivariate analysis to track changes in community structure. A total of 69 families and 134,693 organisms were identified. The investigated metrics were mainly affected by the coefficient of variation of discharge, minimum discharge, ammonium, and temperature. The short-term dynamics of hydrological and physico-chemical variables were generally less important than the overall random effects as drivers of macroinvertebrate-based metrics. However, the relevance of a random effect (site, time, their interaction) differed depending on the biological metrics analysed. Understanding potential differences in response to short term and short stretch conditions would benefit biomonitoring and restoration procedures in both regulated and natural rivers
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