The weight for random quark masses

In theories in which the parameters of the low energy theory are not unique, perhaps having different values in different domains of the universe as is possible in some inflationary models, the fermion masses would be distributed with respect to some weight. In such a situation the specifics of the fermion masses do not have a unique explanation, yet the weight provides the visible remnant of the structure of the underlying theory. This paper introduces this concept of a weight for the distribution of masses and provides a quantitative estimate of it from the observed quarks and leptons. The weight favors light quark masses and appears roughly scale invariant (rho ~ 1/m). Some relevant issues, such as the running of the weight with scale and the possible effects of anthropic constraints, are also discussed.Comment: 35pages, 19 figure

Impact of Environmental Factors on Bacteriocin Promoter Activity in Gut-Derived Lactobacillus salivarius

peer-reviewedBacteriocin production is regarded as a desirable probiotic trait that aids in colonization and persistence in the gastrointestinal tract (GIT). Strains of Lactobacillus salivarius, a species associated with the GIT, are regarded as promising probiotic candidates and have a number of associated bacteriocins documented to date. These include multiple class IIb bacteriocins (salivaricin T, salivaricin P, and ABP-118) and the class IId bacteriocin bactofencin A, which show activity against medically important pathogens. However, the production of a bacteriocin in laboratory media does not ensure production under stressful environmental conditions, such as those encountered within the GIT. To allow this issue to be addressed, the promoter regions located upstream of the structural genes encoding the L. salivarius bacteriocins mentioned above were fused to a number of reporter proteins (green fluorescent protein [GFP], red fluorescent protein [RFP], and luciferase [Lux]). Of these, only transcriptional fusions to GFP generated signals of sufficient strength to enable the study of promoter activity in L. salivarius. While analysis of the class IIb bacteriocin promoter regions indicated relatively weak GFP expression, assessment of the promoter of the antistaphylococcal bacteriocin bactofencin A revealed a strong promoter that is most active in the absence of the antimicrobial peptide and is positively induced in the presence of mild environmental stresses, including simulated gastric fluid. Taken together, these data provide information on factors that influence bacteriocin production, which will assist in the development of strategies to optimize in vivo and in vitro production of these antimicrobials.This work was funded by a SFI PI award “Obesibiotics” (11/PI/1137) to PD

ΦCrAss001 represents the most abundant bacteriophage family in the human gut and infects Bacteroides intestinalis

peer-reviewedCrAssphages are an extensive and ubiquitous family of tailed bacteriophages, predicted to infect bacteria of the order Bacteroidales. Despite being found in ~50% of individuals and representing up to 90% of human gut viromes, members of this viral family have never been isolated in culture and remain understudied. Here, we report the isolation of a CrAssphage (ΦCrAss001) from human faecal material. This bacteriophage infects the human gut symbiont Bacteroides intestinalis, confirming previous in silico predictions of the likely host. DNA sequencing demonstrates that the bacteriophage genome is circular, 102 kb in size, and has unusual structural traits. In addition, electron microscopy confirms that ΦcrAss001 has a podovirus-like morphology. Despite the absence of obvious lysogeny genes, ΦcrAss001 replicates in a way that does not disrupt proliferation of the host bacterium, and is able to maintain itself in continuous host culture during several weeks

Winter habitat selection by marsh tits Poecile palustris in a British woodland

Capsule: Radio-tracking and remote sensing showed Marsh Tits selected for English Oak Quercus robur trees within large winter home-ranges. Aims: To investigate winter habitat selection by Marsh Tits in a British wood, testing for preferences in tree species and woodland structure. Methods: Thirteen Marsh Tits were radio-tracked during the winter, and home-ranges were derived. Lidar and hyperspectral data were used to compare the vegetation structure and tree species composition of entire home-ranges and the core areas of intensive use within. Instantaneous sampling observations provided further information for tree species utilization. Results: The mean home-range was very large (39 ha, n=13). There were no significant differences in mean tree height, canopy closure, understorey height, or shrub volume between full home-ranges and the core areas of use. Core areas contained a significantly greater proportion of English Oak relative to availability in the full home-ranges. Instantaneous sampling confirmed that English Oak was used significantly more than other trees. Conclusion: Selection for English Oak during winter contrasted with previous studies of breeding territories, indicating that habitat usage varies seasonally and demonstrating the need for habitat selection studies throughout the year. Large home-ranges help to explain the Marsh Tit’s sensitivity to habitat fragmentation

Periodic orbit resonances in layered metals in tilted magnetic fields

The frequency dependence of the interlayer conductivity of a layered Fermi liquid in a magnetic field which is tilted away from the normal to the layers is considered. For both quasi-one- and quasi-two-dimensional systems resonances occur when the frequency is a harmonic of the frequency at which the magnetic field causes the electrons to oscillate on the Fermi surface within the layers. The intensity of the different harmonic resonances varies significantly with the direction of the field. The resonances occur for both coherent and weakly incoherent interlayer transport and so their observation does not imply the existence of a three-dimensional Fermi surface.Comment: 4 pages, RevTeX + epsf, 2 figures. Discussion of other work revised. To appear in Phys. Rev. B, Rapid Commun., October 1

Listeriolysin S, a Novel Peptide Haemolysin Associated with a Subset of Lineage I Listeria monocytogenes

peer-reviewedStreptolysin S (SLS) is a bacteriocin-like haemolytic and cytotoxic virulence factor that plays a key role in the virulence of Group A Streptococcus (GAS), the causative agent of pharyngitis, impetigo, necrotizing fasciitis and streptococcal toxic shock syndrome. Although it has long been thought that SLS and related peptides are produced by GAS and related streptococci only, there is evidence to suggest that a number of the most notorious Gram-positive pathogenic bacteria, including Listeria monocytogenes, Clostridium botulinum and Staphylococcus aureus, produce related peptides. The distribution of the L. monocytogenes cluster is particularly noteworthy in that it is found exclusively among a subset of lineage I strains; i.e., those responsible for the majority of outbreaks of listeriosis. Expression of these genes results in the production of a haemolytic and cytotoxic factor, designated Listeriolysin S, which contributes to virulence of the pathogen as assessed by murine- and human polymorphonuclear neutrophil–based studies. Thus, in the process of establishing the existence of an extended family of SLS-like modified virulence peptides (MVPs), the genetic basis for the enhanced virulence of a proportion of lineage I L. monocytogenes may have been revealed.Work is funded by the Irish Government under the National Development Plan, through a Science Foundation Ireland Investigator award to CH, PR and PC (06/IN.1/B98)