Beberapa Faktor Yang Berhubungan Dengan Penularan Schistosoma Japonicum Di Dataran Tinggi Napu Kabupaten Poso Sulawesi Tengah

. The transmission of schistosomiasis is related with human behavior or habit factors in the schistosomiasis endemic area. The Purpose of this study was to identify several factors in relationship with schistosomiasis in Napu Highland. The Study was observational with cross sectional design. Data collection were coducted stool survey and interviewes in among 196 respondents using simple random sampling. Stool samples were examined by the Kato-Katz method and interviewed was used questionnaire to identify several factors in realtionship with schistosomiasis transmission. Out of 6 community behaviours suspected to contribute to infection of schistosomiasis, 5 were related with schistosomiasis, i. e. the habit of taking a bath and washing in the river (p=<0.001), always going to the focus area (p=0.04),utilizing water resources from well (p=0.001), washing legs or hand in the river(p=<0.001) and swimming (p=0.007). Schistosomiasis infection was related to the community behaviour of bathing and washing in the river, always going to the focus area, utilizing water resources from well, washing legs or hand in the river and swimming

Efektivitas Ekstrak Daun Mimba (Azadirachta Indica a. Juss) Terhadap Pertumbuhan Koloni Alternaria Porri Penyebab Penyakit Bercak Ungu Pada Bawang Wakegi (Allium X Wakegi Araki) Secara in Vitro

Purple blotch disease ( A. porri ) is to known as a main disease on onions it has becomes and has become endemic in central planting and it creates a lot of financial lose to farmer. Using neem leaf as a potential alternative pesticide can control the purple blotch on onion. The aim of this research was to determine the inhibition of neem leaf extract toward of fungal pathogens A. porri. This research was conducted in Laboratory of Plant Diseases Faculty of Agriculture, Tadulako University Central Sulawesi Palu. This research was conducted on August until November 2015. Method of this research was mixing neem leaf extract concentrate of 0.4% , 0.6% , 0,8% and 1% into the PDA. Results of this research show that neem leaf extract which is concentrate 1% more effective to press the inhibitory growth of A. porri was 43.33%

Uji Daya Hambat Ekstrak Daun Ketepeng Cina (Cassia Alata L.) Terhadap Jamur Patogen Phytophthora Palmivora Penyebab Penyakit Busuk Buah Kakao (Theobromae Cacao L.)

The study aimed is to find out the inhibitation level of leaves extract of Ketepeng China (Cassia alata L.) insome concentrations on the growth of Phytophthora palmivora funguson cocoa fruit. This study was arranged in Complete Randomized Design, with the extract concentrations tested were 0,5%, 1%, 1,5%, 2% and 2,5% on the growth of Phytophthora palmivora fungus diameter. This study was conducted on September to October 2015 in Pest and Disease Department Laboratory, Agriculture Faculty, Tadulako University. The result showed that the average extract concentration of 2,5% can better inhibitate the growth of Phytophthora palmivora fungus diameter (30.18%), and based on the analysis using simple linear regression, it showed that the concentration 2,5% has the higest dependent variable value with 30,48%

Situasi Filariasis Setelah Pengobatan Massal Tahun Ketiga di Kabupaten Mamuju Utara

Mass Drug Administration (MDA) of lymphatic filariasis has been carried out for three years in North of Mamuju District. However, achievement of efficacy of this programme was unknown. The implementation of mass treatment to filariasis have been conducted for three years in the District of North Mamuju, but achievement of efficacy of this activity were not known yet. To determine the change of filarial situation as well as knowledge, attitude and behavior of the people to filariasis, after three years mass drug treatment, studies were conducted in March-November 2015, the activities were to collect mass blood fingerprick and interview to the local community. The blood fingerprick was conducted to two selected villages do to age 5 years up old (≥ 5 years), and interviewed was conducted to thirty selected villages do to age 15 years up old (≥ 15 years). The results showed that microfilaria rate in North Mamuju district was 1,39%, and the species was Brugiamalayi. Interviewed to 1,586 respondents indicated that knowledge of filariasis disease and the mass treatment it self were low, similary with the behavior related prevention to drug consumption. On the other hand their behave to prevention, control and filariasis treatment were positif. Microfilaria rate was still higher than 1%, as well as knowledge, attitude and behavior related to prevention and mass drug consumption were particularly still low, those indicated that after three years of mass drug treatment implementation the result did not showed as excpected. It suggested that mass drug implementation in North Mamuju need to be continued until five years,with right procedures and seriously monitoring to the area with chronic and microfilaria positive cases

Molecular Microbial Analysis of Lactobacillus Strains Isolated from the Gut of Calves for Potential Probiotic Use

The intestinal microbiota has an influence on the growth and health status of the hosts. This is of particular interest in animals reared using intensive farming practices. Hence, it is necessary to know more about complexity of the beneficial intestinal microbiota. The use of molecular methods has revolutionized microbial identification by improving its quality and effectiveness. The specific aim of the study was to analyze predominant species of Lactobacillus in intestinal microbial ecosystem of young calves. Forty-two lactic acid bacteria (LAB) isolated from intestinal tract of young calves were characterized by: Amplified Ribosomal DNA Restriction Analysis (ARDRA), by using Hae III, Msp I, and Hinf I restriction enzymes, and 16S rDNA gene sequencing. ARDRA screening revealed nine unique patterns among 42 isolates, with the same pattern for 29 of the isolates. Gene fragments of 16S rDNA of 19 strains representing different patterns were sequenced to confirm the identification of these species. These results confirmed that ARDRA is a good tool for identification and discrimination of bacterial species isolated from complex ecosystem and between closely related groups. This paper provides information about the LAB species predominant in intestinal tract of young calves that could provide beneficial effects when administered as probiotic

A case report in cardiovascular magnetic resonance: the contrast agent matters in amyloid

BACKGROUND: Cardiac amyloidosis is a progressive but underdiagnosed and underappreciated cause of heart failure. In the last few years, cardiovascular magnetic resonance (CMR) has become the gold standard for non invasive diagnosis of cardiac amyloidosis with the characteristic subendocardial late gadolinium enhancement. CASE PRESENTATION: We describe a case of a patient who, in the process of aligning protocols for a trial between different centers, had a paired study with two different contrast agents, Dotarem® and MultiHance®. MultiHance® surprisingly failed to demonstrate the characteristic imaging pattern, showing only non specific late gadolinium enhancement at the inferior right ventricular insertion point and different myocardial extracellular volume fraction compared to the one obtained with Dotarem®. MultiHance® is used by many centres, because its partial blood protein binding is a strength for MR angiography, but late gadolinium enhancement, particularly non-ischemic, appears to be compromised. CONCLUSIONS: This case report suggests that contrast agents should be selected with caution, especially with new therapies lining up for amyloid and CMR being used as exploratory end point in clinical trials

Texture Analysis of Cardiovascular Magnetic Resonance Cine Images differentiates etiologies of left ventricular hypertrophy

BACKGROUND: Textural analysis (TA) shows promise as radiological biomarker. The use of native TA in the field of cardiology is unproven. We hypothesized that Cardiovascular Magnetic Resonance pre-contrast bSSFP cine images could be analysed using TA software; TA features would differentiate different aetiologies of disease causing increased myocardial wall thickness (left ventricular hypertrophy {LVH}) and indicate the severity of myocardial tissue abnormality. METHOD: A mid short axis pre-contrast cine frame of 216 cases (50 hypertrophic cardiomyopathy (predominantly LVOTO sub type) (HCM), 52 cardiac amyloid (predominantly AL sub-type) (CA), 68 aortic stenosis (AS), 15 hypertensive with LVH (HTN+LVH) and 31 healthy volunteers (HV)) underwent CMRTA using TexRAD (TexRAD Ltd, Cambridge, UK). Among HV, 16/ 31 were scanned twice to form a test-retest reproducibility cohort. CMRTA comprised a filtration-histogram technique to extract and quantify features using 6 parameters. RESULTS: Test-retest analysis in HV showed a medium filter (3mm) was the most reproducible (intra-class correlation of 0.9 for kurtosis and skewness and 0.8 for mean and SD). Disease cohorts were statistically different (p<0.001) to health for all parameters. Pair wise comparisons of CMRTA parameters showed kurtosis and skewness consistently significant in ranking degree of difference from HV (greatest to least); CA, HCM, LVH+HTN, AS (p<0.001). Similarly mean, SD, entropy and mean positive pixel (MPP) were consistent in ranking degree of difference from HV; HCM, CA, AS and HTN+LVH. CONCLUSION: Radiomic features of bSSFP CMR data sets, derived using TA, show promise in discriminating between aetiologies of LVH