EPIdemiology of Surgery-Associated Acute Kidney Injury (EPIS-AKI) : Study protocol for a multicentre, observational trial

More than 300 million surgical procedures are performed each year. Acute kidney injury (AKI) is a common complication after major surgery and is associated with adverse short-term and long-term outcomes. However, there is a large variation in the incidence of reported AKI rates. The establishment of an accurate epidemiology of surgery-associated AKI is important for healthcare policy, quality initiatives, clinical trials, as well as for improving guidelines. The objective of the Epidemiology of Surgery-associated Acute Kidney Injury (EPIS-AKI) trial is to prospectively evaluate the epidemiology of AKI after major surgery using the latest Kidney Disease: Improving Global Outcomes (KDIGO) consensus definition of AKI. EPIS-AKI is an international prospective, observational, multicentre cohort study including 10 000 patients undergoing major surgery who are subsequently admitted to the ICU or a similar high dependency unit. The primary endpoint is the incidence of AKI within 72 hours after surgery according to the KDIGO criteria. Secondary endpoints include use of renal replacement therapy (RRT), mortality during ICU and hospital stay, length of ICU and hospital stay and major adverse kidney events (combined endpoint consisting of persistent renal dysfunction, RRT and mortality) at day 90. Further, we will evaluate preoperative and intraoperative risk factors affecting the incidence of postoperative AKI. In an add-on analysis, we will assess urinary biomarkers for early detection of AKI. EPIS-AKI has been approved by the leading Ethics Committee of the Medical Council North Rhine-Westphalia, of the Westphalian Wilhelms-University Münster and the corresponding Ethics Committee at each participating site. Results will be disseminated widely and published in peer-reviewed journals, presented at conferences and used to design further AKI-related trials. Trial registration number NCT04165369

Pea (Pisum sativum) and faba beans (Vicia faba) in dairy cow diet: effect on milk production and quality

The use of alternative plant proteins in place of the soybean meal protein in diets for producing animals aims to reduce the extra-EU soybean import and partially substitute the GMO in the food chain. Among possible alternatives, the heat-processed legume grains seem interesting for dairy cow diets. Two consecutive experiments were carried out to evaluate flaked pea and faba beans as substitute for soybean meal in diets for Reggiana breed dairy cows producing milk for Parmigiano- Reggiano cheese-making. In both experiments a C concentrate (110 g/kg soybean meal, no pea and faba beans) was compared to a PF concentrate (150 g/kg flaked pea, 100 g/kg flaked faba beans, no soybean meal). Forages fed to animals were hay (mixed grass and alfalfa) in experiment 1 and hay plus mixed grass in experiment 2. Concentrate intake, milk yield and milk quality (rennet coagulation traits included) were similar between feeding groups. Parameters on the grab faecal samples, as empirical indicators of digestibility, had a smaller (P<0.01) amount of residual concentrate in the PF group compared to the C group (2.4 vs 3.1 and 2.3 vs 2.8%, respectively for PF and C in experiment 1 and 2). Some blood indicators of nitrogen metabolism (protein, albumin, urea) were similar between the feeding groups.The inclusion of pea and faba beans, within the allowed limit of the Parmigiano-Reggiano Consortium for diet formulation, could represent a feasible opportunity for a total substitution of soybean

Cytogentic relations among the genera of the subfamily Pitheciinae (Cebidae, Primates)

Universidade Federal do Pará-UFPA, CAPES, CNPq, FADESP, FINEP and C&T/PPG-7Universidade Federal do Pará. Departamento de Genética. Laboratório de Citogenética. Belém, PA, Brazil.Ministério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil.Ministério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil.Centro de Primatologia do Rio de Janeiro. Rio de Janeiro RJ, Brazil.Universidade Federal do Pará. Departamento de Genética. Laboratório de Citogenética. Belém, PA, Brazil.Universidade Federal do Pará. Departamento de Genética. Laboratório de Citogenética. Belém, PA, Brazil.Universidade Federal do Pará. Departamento de Genética. Laboratório de Citogenética. Belém, PA, Brazil.Universidade Federal do Pará. Departamento de Genética. Laboratório de Citogenética. Belém, PA, Brazil.A comparative chromosomal analysis was carried out in some specimens of the three genera of the subfamily Pitheciinae (Pithecia, Chiropotes and Cacajao) using a classic cytogenetic technique (G-banding). The three genera present distinct 2n: Pithecia presents 2n=48, Chiropotes 2n=54 and Cacajao 2n=45 in the males and 2n=46 in the females. The difference in 2. found in Cacajao occurs due to a y-autosome translocation present in the male of Cacajao calvus rubicundus. Species and subspecies of the same genera show the same 2n, however the FN are different. G-banding was used to analyze the homeologies among the taxa studied and, by this mean, we observed the presence of speciesspecific chromosomes, chromosomes shared between two or among all the taxa and also chromosomes that are present in all the specimens studied. Data of this karyotypic analysis suggest that Pithecia is the most primitive among the pithecins, followed by Chiropotes, and Cacajao is the most recent one, with very rearranged karyotype

Comportamento e composi\ue7\ue3o de um grupo de Callithrix jacchus Erxleben (Primates, Callitrichidae) na mata de Dois Irm\ue3os, Recife, Pernambuco, Brasil

<abstract language="eng">The objective of this study was to determine the size and composition of a group of wild Callithrix jacchus Erxleben, 1777, a small neotropical primate from the North East Brazil. The study was carried out in Dois Irmãos Forest, Recife, Pernambuco, Brazil, in an area of 6ha where this social group of marmosets had its home range. A graded transects system was cut to provide the access and moving during the observation period. To provide an individual identification for the members of the marmosets group and to achieve the objective of this study, two captures were made, with a six month interval between them in a 50x50m² area at the nuclear range of the group's area. The animals were realeased after a maximum period of 24h after traping. We made, during the whole study period, direct observations of the social group which wasn't limited only to count and identification of the marmosets, but expanded in consideration on the ecology and behavior of the Callithrix jacchus group. The results show us that the size and composition of the study group was similar to others descriptions for the Callitrichidae. The group composition suffered a 30% "turnover" in a six month interval, and the adults particulary, had a big frequency of change. One pair of infants was seen in December 1986 - in the middle of the dry season - and another infant appeared in the study group in 1987, just after the end of the wet season. Some dietary items were fruits, leaf buds and also exudate from the trunks and branchs of some tree's species. Intergroup agonistic interactions were recorded during February 1987