Direct detection of Campylobacter from feces of organic and conventional pigs highlighted the presence of Campylobacter lanienae

In the frame of the CORE Organic II funded European project SafeOrganic, fecal samples from 31 organic pig herds and 31 conventional pig herds were sampled in a slaughterhouse. Samples were highly positive in Campylobacter coli but also in another Campylobacter species not described at that time in France. Identification by MALDI-TOF mass spectrometry and PCR 16S allowed us to confirm that 85 isolates were C. Lanienae; 56 from conventional pigs and 29 from organic pigs. Individual occurrence in Campylobacter spp. was thus re-estimated to 91.1 % (51/56) and 96.3 % (52/54) for conventional and organic pigs, respectively. A total of 55 isolates of C. Lanienae were studied for their resistance to 7 antibiotics. Only one was pansusceptible. Natural resistance to Nalidixic acid was confirmed. Resistance to Tetracycline was significantly different (p \u3c to 0,001) between the two productions: 88 % of isolates from conventional pigs were resistant against 14% of isolates from organic pigs. Moreover, isolates from conventional pigs were mostly multiresistant (73%) whereas only 5% of strains isolated in organic pigs were multiresistant. The C. lanienae isolates were typed by PFGE using KpnI and SmaI enzymes. The genetic diversity was very high, whatever the enzyme used. No link between PFGE profile and isolate origin or antibiotic resistance pattern was evidenced. This study allowed us to demonstrate for the first time in France that pigs, known to be a reservoir for C. coli may also carry in their feces a species rarely highlighted: C. lanienae. The species was present in fecal samples from conventional and organic pigs. The lower level of antibiotic resistance and multiresistance of C. Lanienae strains for organic pigs may be related to the restricted use of antibiotics in this production

Parametrization of the angular correlation and degree of linear polarization in two-photon decays of hydrogen-like ions

The two-photon decay in hydrogen-like ions is investigated within the framework of second order perturbation theory and Dirac's relativistic equation. Special attention is paid to the angular correlation of the emitted photons as well as to the degree of linear polarization of one of the two photons, if the second is just observed under given angles. Expressions for the angular correlation and the degree of linear polarization are expanded in terms of $\cos\theta$-polynomials, whose coefficients depend on the atomic number and the energy sharing of the emitted photons. The effects of including higher (electric and magnetic) multipoles upon the emitted photon pairs beyond the electric-dipole approximation are also discussed. Calculations of the coefficients are performed for the transitions $2s_{1/2}\rightarrow1s_{1/2}$, $3d_{3/2}\rightarrow1s_{1/2}$ and $3d_{5/2}\rightarrow1s_{1/2}$, along the entire hydrogen isoelectronic sequence ($1\le Z \le 100$)

Investigating parental care behaviour in same-sex pairing of zoo greater flamingo ( Phoenicopterus roseus )

This is the final version of the article. Available from PeerJ via the DOI in this record.Same-sex pair bonds have been documented in several animal species and they are widespread in birds. However, little is known about the evolutionary origin and the adaptive value of such behaviour. The aim of this study was to investigate the parental behaviour of four zoo female greater flamingos involved in two breeding pairs, housed in a flock at Parco Natura Viva, Italy. Further, the behaviour of the study females was compared with that of male and female flamingos in heterosexual pairs described in a previous published work on this same flock. For each pair, the behaviour of both birds during the incubation period was recorded and twenty 10-minute sessions were run within the incubation period. A continuous focal animal sampling method was used to collect data on location (on the nest or not on the nest) and the parental care behaviour (e.g.: agonistic behaviours toward disturbing conspecifics, egg-care, nest-building, self-comfort behaviour, sleeping) of the two pairs. Data of the current study females were compared with those of females and males involved in heterosexual pairs of this same flock. Results showed that within each pair the egg-layer female stayed away from the nest more than the other female. In addition, the female that did not lay an egg was more involved in agonistic behaviour compared to other females, particularly when in specific locations. In heterosexual pairs, male flamingos were more involved in the incubation and in nest protection. Moreover, no significant differences in the time spent on the nest and away from the nest between the heterosexual male and the non-layer females of same-sex pairs were found. The same findings were reported when comparing heterosexual females and the egg-layer females of the same-sex pairs. Therefore, our findings suggest that in greater flamingos the behaviour of the female–female pairs seems to be equivalent to that of male-female bonds. Such research provides more insight into flamingo social behaviour, and their reproductive cycle, and provides information on why pair bonds may form and how these affect the wider breeding behaviour of the flock

Comparison of organic and conventional pig productions on prevalence, antibiotic resistance and genetic diversity of Escherichia coli

The objectives of this study were to assess the prevalence, the tetracycline resistance level and the genetic diversity of Escherichia coli isolated from organic pigs in comparison with conventional pigs. This study is integrated in a global European project, Safeorganic, funded trough CORE Organic II call. 25 organic and 25 conventional herds were considered in one slaughterhouse from April to October 2012. Colon content of 2 pigs per herds was sampled. For each pig, enumeration of E. coli and of tetracycline resistant E. coli (TET+E. coli) was determined

Virtual light-by-light scattering and the g factor of a bound electron

The contribution of the light-by-light diagram to the g factor of electron and muon bound in Coulomb field is obtained. For electron in a ground state, our results are in good agreement with the results of other authors obtained numerically for large Z. For relatively small Z our results have essentially higher accuracy as compared to the previous ones. For muonic atoms, the contribution is obtained for the first time with the high accuracy in whole region of Z.Comment: 10 pages, 3 figures, RevTe

Structural and functional brain changes following four weeks of unimanual motor training: evidence from fMRI-guided diffusion MRI tractography

We have reported reliable changes in behaviour, brain structure and function in 24 healthy right-handed adults who practiced a finger-thumb opposition sequence task with their left hand for 10 mins daily, over four weeks. Here we extend these findings by employing diffusion MRI to investigate white-matter changes in the corticospinal tract, basal-ganglia, and connections of the dorsolateral prefrontal cortex. Twenty-three participant datasets were available with pre-training and post-training scans. Task performance improved in all participants (mean: 52.8%, SD: 20.0%; group

Relativistic total cross section and angular distribution for Rayleigh scattering by atomic hydrogen

We study the total cross section and angular distribution in Rayleigh scattering by hydrogen atom in the ground state, within the framework of Dirac relativistic equation and second-order perturbation theory. The relativistic states used for the calculations are obtained by making use of the finite basis set method and expressed in terms of B-splines and B-polynomials. We pay particular attention to the effects that arise from higher (non-dipole) terms in the expansion of the electron-photon interaction. It is shown that the angular distribution of scattered photons, while it is symmetric with respect to the scattering angle $\theta$=90$^\circ$ within the electric dipole approximation, becomes asymmetric when higher multipoles are taken into account. The analytical expression of the angular distribution is parametrized in terms of Legendre polynomials. Detailed calculations are performed for photons in the energy range 0.5 to 10 keV. When possible, results are compared with previous calculations.Comment: 8 pages, 5 figure

How much laser power can propagate through fusion plasma?

Propagation of intense laser beams is crucial for inertial confinement fusion, which requires precise beam control to achieve the compression and heating necessary to ignite the fusion reaction. The National Ignition Facility (NIF), where fusion will be attempted, is now under construction. Control of intense beam propagation may be ruined by laser beam self-focusing. We have identified the maximum laser beam power that can propagate through fusion plasma without significant self-focusing and have found excellent agreement with recent experimental data, and suggest a way to increase that maximum by appropriate choice of plasma composition with implication for NIF designs. Our theory also leads to the prediction of anti-correlation between beam spray and backscatter and suggests the indirect control of backscatter through manipulation of plasma ionization state or acoustic damping.Comment: 15 pages, 4 figures, submitted to Plasma Physics and Controlled Fusio

Angular distribution studies on the two-photon ionization of hydrogen-like ions: Relativistic description

The angular distribution of the emitted electrons, following the two-photon ionization of the hydrogen-like ions, is studied within the framework of second order perturbation theory and the Dirac equation. Using a density matrix approach, we have investigated the effects which arise from the polarization of the incoming light as well as from the higher multipoles in the expansion of the electron--photon interaction. For medium- and high-Z ions, in particular, the non-dipole contributions give rise to a significant change in the angular distribution of the emitted electrons, if compared with the electric-dipole approximation. This includes a strong forward emission while, in dipole approxmation, the electron emission always occurs symmetric with respect to the plane which is perpendicular to the photon beam. Detailed computations for the dependence of the photoelectron angular distributions on the polarization of the incident light are carried out for the ionization of H, Xe$^{53+}$, and U$^{91+}$ (hydrogen-like) ions.Comment: 16 pages, 4 figures, published in J Phys

Electric dipole-forbidden nuclear transitions driven by super-intense laser fields

Electric dipole-forbidden transitions of nuclei interacting with super-intense laser fields are investigated considering stable isotopes with suitable low-lying first excited states. Different classes of transitions are identified, and all magnetic sublevels corresponding to the near-resonantly driven nuclear transition are included in the description of the nuclear quantum system. We find that large transition matrix elements and convenient resonance energies qualify nuclear M1 transitions as good candidates for the coherent driving of nuclei. We discuss the implications of resonant interaction of intense laser fields with nuclei beyond the dipole approximation for the controlled preparation of excited nuclear states and important aspects of possible experiments aimed at observing these effects.Comment: 20 pages, 2 tables, 3 figures, minor modifications and update to the published versio