Prevelance of Emeria Genera Upon Coccidiosis Infection Toward Male Layer

Chicken is one of the bird species used as a source of animal protein which is very enjoyable and known by society. One chicken species that widely cultivated are the male layer. However, there was frequently obstacle in the maintenance, one is caused by parasite species of coccidia that causes coccidiosis. This research is conducted in November 2016 on a livestock farm, Kelurahan Segalamider, Kecamatan Tanjung Karang Barat, Kota Bandar Lampung. This research aims to determine the type of parasite egg that causes coccidiosis and to determine the prevalence of coccidia attack on the male layer. This research uses a proportionate clustered random sampling method, which took 100 samples of the male layer faces randomly from each cage as much as 25 samples. The identification using the guide book of Conway and McKenzie (2007) and the calculation of the number of parasite eggs using EPG Mc. Master assay in Balai Veteriner Lampung. Data were analyzed descriptively by displaying data in tables and figures. The result showed that there are five species of Eimeria, namely Eimeria tenella, Eimeria maxima, Eimeria brunetti, Eimeria nikamae, and Eimeria mitis that found in oocyst sporulated form and oocyst not sporulated. The parasite causes coccidiosis in the male layer is Eimeria tenella with prevalence values of coccidia attack by 20%

Proteolytic Activity of the Entomopathogenic Fungi (Penicillium SP. ) of Cockroaches (Periplaneta Americana)

Cockroaches (Periplaneta americana) were the insect vectors of disease that caused adverse effects on human health. Control cockroaches\u27 excessive use of insecticides can lead to residue in the environment and resistance cockroach. Therefore it was necessary to use an alternative such as entomopathogenic fungal as biological agents. The entomopathogenic fungi penetrated via the integument of a cockroach to reach the hemocoel. Proteins were the molecules responsible for integument strength in the cockroach, It was synthesis the proteases to degrading proteins. The study begins with the isolation of entomopathogenic fungi using the moist chamber method with the cockroach as insect bait. The fungus that grows on cockroaches are cultured and purified on Potato Dextrose Agar (PDA) medium then identified. Identification was carried out through macroscopic observations including colony color and diameter and microscopic observations including conidia, conidiophores, hyphae, vesicles, fialids, and leg cells. The result of isolation and identification obtained as Penicillium sp. Proteases enzymatic activity tested on PDA with anlene 1%. The clear zone formed is measured to show the activity of proteases produced by Penicillium sp

Maya Index Analysis of Dengue Fever Vector in East Metro Sub-District Lampung Province Indonesia

Dengue fever was one of the dangerous diseases due to it might cause death in a short time. It transmitted through its vectors, namely, Aedes aegypti and Aedes albopictus. Maya index is an indicator that used to identify a high-risk area or not as a breeding ground for Aedes sp. The purpose of this study was to determine the type of landfill which has the potential as a breeding ground for mosquitoes, to find out the larvae found in various landfill sites, and to know the Maya index status in Metro Timur Subdistrict which was carried out in November-December 2017. This research conducted by a direct survey of 100 residents\u27 houses in East Metro. The results indicated that the type of controlled water reservoir that had the most potential as a breeding place for mosquitoes was a bathtub, while the type of water reservoir that was not controlled which had the potential as a place for mosquito breeding was a fish pond usage. There were 2 types of larvae found, namely, Aedes aegypti larvae with a percentage of 47.7% and Aedes albopictus larvae with a percentage of 52.3%. The Maya index status detected there, was a medium category, which obtained from the BRI combination and HRI category. Besides, it did not mean that the area has been free from dengue cases. The role of the community is still very much needed in eradicating landfills which has the potential as a breeding place for dengue mosquitoes vector

Determination of Aedes Spp. Mosquito Breeding Spots as a High-Risk Area Through Maya Index Analysis in Way Halim Bandar Lampung

Dengue Haemorrhagic Fever (DHF) is a disease that is transmitted through the Aedes aegypti and Aedes albopictus mosquito vectors. The Maya index (MI) is an indicator to identify whether an area is at high risk as a breeding site for mosquitoes. This study aims to determine the types of breeding places and types of mosquito larvae of DHF and MI vectors in the Way Halim District of Bandar Lampung. The method used was a survey method on 100 houses in 5 villages in Way Halim District, namely Jaga Baya 1, Jaga Baya 2, Jaga Baya 3, Way Halim Permai, and Gunung Sulah. Data analysis to determine MI uses the Breeding Risk Index (BRI) and Hygiene Risk Indicator (HRI) indicators. The results of this study indicate that there were 225 containers inside the house observed, where 51 containers of them were found to have Aedes mosquito larvae while outside the house found as many as 28 containers with 8 containers including larvae. The identification results found 2 types of Aedes mosquitoes namely, Ae. aegypti (88.24%) and Ae. albopictus (11.76%). The results of the MI analysis show that Way Halim District is classified as a District with a moderate MI category with an MI value of 46%

Uji Efektivitas Bacillus thuringiensis var. Israelensis yang Telah Kedaluwarsa Terhadap Larva Nyamuk Aedes aegypti

ABSTRAKPenelitian ini bertujuan untuk mengetahui konsentrasi efektif dan mortalitas Bti kedaluwarsa terhadap larva nyamuk Ae. aegypti. Penelitian ini dilaksanakan pada bulan Desember 2018 di Laboratorium Zoologi II, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lampung. Rancangan penelitian yaitu RAL (Rancangan Acak Lengkap) menggunakan Bti kedaluwarsa sebagai faktor utama dengan 6 taraf konsentrasi : 0 ppm (kontrol positif), 50 ppm, 100 ppm, 150 ppm, 200 ppm, 250 ppm, 300 ppm dan Bti normal 20 ppm sebagai kontrol negatif yang terdiri dari 4 ulangan. Parameter yang di uji adalah tingkat mortalitas larva Ae. aegypti. Data di olah dengan uji ANOVA dan dilanjut uji BNT pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa konsentrasi Bti kedaluwarsa 300 ppm merupakan konsentrasi Bti kedaluwarsa yang paling tinggi dan lamanya waktu kontak yang paling cepat menyebabkan kematian larva Ae. aegypti hingga 100%. Kesimpulannya Bti kedaluwarsa masih efektif dan memiliki pengaruh terhadap kematian larva Ae. aegypti. Kata kunci : Ae. aegypti , Bacillus thuringiensis var. Israelensis, Mortalitas ABSTRACTThis study aims to determine the effective concentration and mortality of Bti who have expired mosquito larvae Ae. aegypti. This research was conducted in December 2018 at the Zoology Laboratory II, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung. This research was conducted with RAL (Complete Random Design) using Bti expiration as the main factor with 6 levels of concentration: 0 ppm (positive control), 50 ppm, 100 ppm, 150 ppm, 200 ppm, 250 ppm, 300 ppm and normal Bti 20 ppm as a negative control consisting of 4 replications. As a parameter is the mortality rate of larvae Ae. aegypti. Data was analyzed using ANOVA test and continued with LSD test at the 5% level. The results showed that the Bti concentration expiry of 300 ppm the highest expiration Bti concentration and the longest contact time to cause death to the larvae Ae. aegypti up to 100%. In conclusion, the Bti expired has an effective and influence on the death of the larvae Ae. aegypti. Keywords: Ae. aegypti, Bacillus thuringiensis var. Israelensis, Mortalit