Impact of tortuous flow on bacteria streamer development in microfluidic system during filtration

The way in which bacterial communities colonize flow in porous media is of importance but basic knowledge on the dynamic of these phenomena is still missing. The aim of this work is to develop microfluidic experiments in order to progress in the understanding of bacteria capture in filters and membranes. PDMS microfluidic devices mimicking filtration processes have been developed to allow a direct dynamic observation of bacteria across 10 or 20 micrometers width microchannels. When filtered in such devices, bacteria behave surprisingly: Escherichia coli, Pseudomonas aeruginosa or Staphylococcus aureus accumulate in the downstream zone of the filter and form large streamers which oscillate in the flow. In this study streamer formation is put in evidence for bacteria suspension in non nutritive conditions in less than one hour. This result is totally different from the one observed in same system with “inert” particles or dead bacteria which are captured in the bottleneck zone and are accumulated in the upstream zone. Observations within different flow geometries (straight channels, connected channels, staggered row pillars) show that the bacteria streamer development is influenced by the flow configuration and, particularly by the presence of tortuosity within the microchannels zone. These results are discussed at the light of 3D flow simulations. In confined systems and in laminar flow there is secondary flow (z-velocities) superimposed to the streamwise motion (in xy plane). The presence of the secondary flow in the microsystems has an effect on the bacterial adhesion. A scenario in three steps is established to describe the formation of the streamers and to explain the positive effect of tortuous flow on the development kinetics

Wetting to Non-wetting Transition in Sodium-Coated C_60

Based on ab initi and density-functional theory calculations, an empirical potential is proposed to model the interaction between a fullerene molecule and many sodium atoms. This model predicts homogeneous coverage of C_60 below 8 Na atoms, and a progressive droplet formation above this size. The effects of ionization, temperature, and external electric field indicate that the various, and apparently contradictory, experimental results can indeed be put into agreement.Comment: 4 pages, 4 postscript figure

MAXI J1659-152: the shortest orbital period black-hole binary

Following the detection of a bright new X-ray source, MAXI J1659-152, a series of observations was triggered with almost all currently flying high-energy missions. We report here on XMM-Newton, INTEGRAL and RXTE observations during the early phase of the X-ray outburst of this transient black-hole candidate. We confirm the dipping nature in the X-ray light curves. We find that the dips recur on a period of 2.4139+/-0.0005 hrs, and interpret this as the orbital period of the system. It is thus the shortest period black-hole X-ray binary known to date. Using the various observables, we derive the properties of the source. The inclination of the accretion disk with respect to the line of sight is estimated to be 60-75 degrees. The companion star to the black hole is possibly a M5 dwarf star, with a mass and radius of about 0.15 M_sun and 0.23 R_sun, respectively. The system is rather compact (orbital separation is about 1.35 R_sun) and is located at a distance of roughly 7 kpc. In quiescence, MAXI J1659-152 is expected to be optically faint, about 28 mag in the V-band.Comment: 5 pages, 4 figures, to be published in the proceedings of the 4th International MAXI Workshop `The First Year of MAXI: Monitoring variable X-ray sources', 2010 Nov 30 - Dec 2, Tokyo, Japa

Hard X-ray emission of the microquasar GX 339-4 in the low/hard state

We present the analysis of the high-energy emission of the Galactic black hole binary GX 339-4 in a low/hard state at the beginning of its 2004 outburst. The data from 273 ks of INTEGRAL observations, spread over 4 weeks, are analyzed, along with the existing simultaneous RXTE HEXTE and PCA data. During this period, the flux increases by a factor of ~=3, while the spectral shape is quite unchanged, at least up to 150 keV. The high-energy data allow us to detect the presence of a high-energy cutoff, generally related to thermal mechanisms, and to estimate the plasma parameters in the framework of the Comptonization models. We found an electron temperature of 60-70 keV and an optical depth of around 2.5, with a rather low reflection factor (0.2-0.4). In the last observation, we detected a high-energy excess above 200 keV with respect to thermal Comptonization, while at lower energies the spectrum is practically identical to the previous one taken just 2 days before. This suggests that the low- and high-energy components have a different origin

Detecting host-parasitoid interactions in an invasive Lepidopteran using nested tagging DNA metabarcoding

Determining the host-parasitoid interactions and parasitism rates for invasive species entering novel environments is an important first step in assessing potential routes for biocontrol and integrated pest management. Conventional insect rearing techniques followed by taxonomic identification are widely used to obtain such data, but this can be time consuming and prone to biases. Here we present a Next Generation Sequencing approach for use in ecological studies which allows for individual level metadata tracking of large numbers of invertebrate samples through the use of hierarchically organised molecular identification tags. We demonstrate its utility using a sample data set examining both species identity and levels of parasitism in late larval stages of the Oak Processionary Moth (Thaumetopoea processionea - Linn. 1758), an invasive species recently established in the UK. Overall we find that there are two main species exploiting the late larval stages of Oak Processionary Moth in the UK with the main parasitoid (Carcelia iliaca - Ratzeburg, 1840) parasitising 45.7% of caterpillars, while a rare secondary parasitoid (Compsilura conccinata - Meigen, 1824) was also detected in 0.4% of caterpillars. Using this approach on all life stages of the Oak Processionary Moth may demonstrate additional parasitoid diversity. We discuss the wider potential of nested tagging DNA-metabarcoding for constructing large, highly-resolved species interaction networks

INTEGRAL and XMM-Newton observations of the weak GRB 030227

We present INTEGRAL and XMM-Newton observations of the prompt gamma-ray emission and the X-ray afterglow of GRB030227, the first GRB for which the quick localization obtained with the INTEGRAL Burst Alert System (IBAS) has led to the discovery of X-ray and optical afterglows. GRB030227 had a duration of about 20 s and a peak flux of 1.1 photons cm^-2 s^-1 in the 20-200 keV energy range. The time averaged spectrum can be fit by a single power law with photon index about 2 and we find some evidence for a hard to soft spectral evolution. The X-ray afterglow has been detected starting only 8 hours after the prompt emission, with a 0.2-10 keV flux decreasing as t^-1 from 1.3x10e-12 to 5x10e-13 erg cm^-2 s^-1. The afterglow spectrum is well described by a power law with photon index 1.94+/-0.05 modified by a redshifted neutral absorber with column density of several 10e22 cm^-2. A possible emission line at 1.67 keV could be due to Fe for a redshift z=3, consistent with the value inferred from the absorption.Comment: 16 pages, 5 figures, latex, Accepted for publication in The Astrophysical Journal Letter