1,103 research outputs found

    Association between avian necrotic enteritis and Clostridium perfringens strains expressing NetB toxin

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    A novel toxin, NetB, has recently been identified in virulent avian Clostridium perfringens isolates and shown to be an essential virulence factor in a clinical necrotic enteritis isolate. To assess whether NetB is more generally associated with avian necrotic enteritis isolates we have screened a range of C. perfringens strains from geographically diverse locations for both the presence and expression of the netB gene. Forty-four isolates were derived from necrotic enteritis disease cases from Australia, Belgium, Denmark and Canada and 55 isolates from healthy chickens from Australia and Belgium. The majority of strains isolated from necrotic enteritis-affected birds were netB positive (70%) and there was an absolute correlation between the presence of netB and in vitro expression of the NetB protein. Only two of the C. perfringens isolates from healthy chickens carried netB. Sequencing of the netB gene from 23 positive isolates showed that NetB is highly conserved, with only one predicted amino acid (A168T) difference, in six isolates, compared to the published sequence. This change did not alter the in vitro activity of the NetB toxin. The gene encoding the recently discovered TpeL toxin was also screened using PCR and only found in a small proportion of NetB-positive isolates from diseased birds. A selection of NetB-negative isolates, originating from diseased birds, was unable to cause disease in a necrotic enteritis induction model. This study provides further evidence that NetB is important in pathogenesis and advances our current understanding of C. perfringens virulence factors in avian necrotic enteritis

    ROSAT HRI X-ray Observations of the Open Globular Cluster NGC 288

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    A ROSAT HRI X-ray image was obtained of the open globular cluster NGC 288, which is located near the South Galactic Pole. This is the first deep X-ray image of this system. We detect a Low Luminosity Globular Cluster X-ray source (LLGCX) RXJ005245.0-263449 with an X-ray luminosity of (5.5+-1.4)x10^32 ergs/s (0.1-2.0 keV), which is located very close to the cluster center. There is evidence for X-ray variability on a time scale of <~ 1 day. The presence of this LLGCX in such an open cluster suggests that dense stellar systems with high interaction rates are not needed to form LLGCXs. We also searched for diffuse X-ray emission from NGC 288. Upper limits on the X-ray luminosities are L_X^h < 9.5x10^32 ergs/s (0.52-2.02 keV) and L_X^s < 9.3x10^32 ergs/s (0.11-0.41 keV). These imply upper limits to the diffuse X-ray to optical light ratios in NGC 288 which are lower than the values observed for X-ray faint early-type galaxies. This indicates that the soft X-ray emission in these galaxies is due either to a component which is not present in globular clusters (e.g., interstellar gas, or a stellar component which is not found in low metallicity Population II systems), or to a relatively small number of bright Low Mass X-ray Binaries (LMXBs).Comment: The Astrophysical Journal in press. Minor revisions to improve presentation. 6 pages with 3 embedded Postscript figures in emulateapj.st

    The First Empirical Mass Loss Law for Population II Giants

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    Using the Spitzer IRAC camera we have obtained mid-IR photometry of the red giant branch stars in the Galactic globular cluster 47 Tuc. About 100 stars show an excess of mid-infrared light above that expected from their photospheric emission. This is plausibly due to dust formation in mass flowing from these stars. This mass loss extends down to the level of the horizontal branch and increases with luminosity. The mass loss is episodic, occurring in only a fraction of stars at a given luminosity. Using a simple model and our observations we derive mass loss rates for these stars. Finally, we obtain the first empirical mass loss formula calibrated with observations of Population II stars. The dependence on luminosity of our mass loss rate is considerably shallower than the widely used Reimers Law. The results presented here are the first from our Spitzer survey of a carefully chosen sample of 17 Galactic Globular Clusters, spanning the entire metallicity range from about one hundredth up to almost solar

    Another Non-segregated Blue Straggler Population in a Globular Cluster: the Case of NGC 2419

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    We have used a combination of ACS-HST high-resolution and wide-field SUBARU data in order to study the Blue Straggler Star (BSS) population over the entire extension of the remote Galactic globular cluster NGC 2419. The BSS population presented here is among the largest ever observed in any stellar system, with more than 230 BSS in the brightest portion of the sequence. The radial distribution of the selected BSS is essentially the same as that of the other cluster stars. In this sense the BSS radial distribution is similar to that of omega Centauri and unlike that of all Galactic globular clusters studied to date, which have highly centrally segregated distributions and, in most cases, a pronounced upturn in the external regions. As in the case of omega Centauri, this evidence indicates that NGC 2419 is not yet relaxed even in the central regions. This observational fact is in agreement with estimated half-mass relaxation time, which is of the order of the cluster age.Comment: in press in the Ap

    BBN and the Primordial Abundances

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    The relic abundances of the light elements synthesized during the first few minutes of the evolution of the Universe provide unique probes of cosmology and the building blocks for stellar and galactic chemical evolution, while also enabling constraints on the baryon (nucleon) density and on models of particle physics beyond the standard model. Recent WMAP analyses of the CBR temperature fluctuation spectrum, combined with other, relevant, observational data, has yielded very tight constraints on the baryon density, permitting a detailed, quantitative confrontation of the predictions of Big Bang Nucleosynthesis with the post-BBN abundances inferred from observational data. The current status of this comparison is presented, with an emphasis on the challenges to astronomy, astrophysics, particle physics, and cosmology it identifies.Comment: To appear in the Proceedings of the ESO/Arcetri Workshop on "Chemical Abundances and Mixing in Stars in the Milky Way and its Satellites", eds., L. Pasquini and S. Randich (Springer-Verlag Series, "ESO Astrophysics Symposia"

    Offshore-onshore record of Last Glacial Maximum-to-present grounding line retreat at Pine Island Glacier, Antarctica

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    Pine Island Glacier, West Antarctica, is the largest Antarctic contributor to global sea-level rise and is vulnerable to rapid retreat, yet our knowledge of its deglacial history since the Last Glacial Maximum is based largely on marine sediments that record retreat to ~120 km downstream of the modern grounding line by the early Holocene. We show, with a suite of 10Be exposure ages from onshore glacial deposits directly adjacent to Pine Island Glacier, that this major glacier thinned rapidly in the early- to mid-Holocene. Our results indicate that Pine Island Glacier was at least 690 m thicker than present prior to ~8 ka. We infer that the rapid thinning detected at the site furthest downstream records the arrival and stabilization of the retreating grounding line at that site by 8-6 ka. By combining our exposure ages and the marine record, we extend knowledge of Pine Island Glacier retreat both spatially and temporally: to 50 km from the modern grounding line and to the mid-Holocene, providing a dataset that is important for future numerical ice sheet model validation

    Sensitive Observations of Radio Recombination Lines in Orion and W51: The Data and Detection of Systematic Recombination Line Blueshifts Proportional to Impact Broadening

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    Sensitive spectral observations made in two frequency bands near 6.0 and 17.6 GHz are described for Orion and W51. Using frequency switching we were able to achieve a dynamic range in excess of 10,000 without fitting sinusoidal or polynomial baselines. This enabled us to detect lines as weak as TA 1mKinthesestrongcontinuumsources.Hydrogenrecombinationlineswith_{A} ~1mK in these strong continuum sources. Hydrogen recombination lines with \Delta n$ as high as 25 have been detected in Orion. In the Orion data, where the lines are stronger, we have also detected a systematic shift in the line center frequencies proportional to linewidth that cannot be explained by normal optical depth effects.Comment: 22 pages, 13 figures. Accepted for publication in Astrophysics and Space Scienc
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