Circ_0007385 promotes the proliferation and inhibits the apoptosis of non-small cell lung cancer cells via miR-337-3p-dependent regulation of LMO3

Background. This study intended to analyze the expression characteristic, functions and underlying mechanism of circular RNA_0007385 (circ_0007385) in non-small cell lung cancer (NSCLC). Methods and Results. RNA and protein expression was examined by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot assay. Cell counting kit 8 (CCK8) assay, colony formation assay, 5- Ethynyl-2’-deoxyuridine (EdU) assay and flow cytometry were applied to analyze cell proliferation ability. Flow cytometry was also conducted to assess cell apoptosis. Dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were performed to verify the predicted target relationships. Xenograft tumor model was utilized to analyze the function of circ_0007385 in vivo, and immunohistochemistry (IHC) assay was used to analyze protein expression in xenograft tumor tissues. Circ_0007385 expression was notably enhanced in NSCLC tissues and cell lines. Circ_0007385 facilitated the proliferation but suppressed the apoptosis of NSCLC cells. Circ_0007385 acted as a sponge for microRNA-337-3p (miR-337-3p), and circ_0007385 overexpression-mediated effects were largely overturned by the overexpression of miR-337-3p in NSCLC cells. MiR-337-3p interacted with the 3’ untranslated region (3’UTR) of LIM-only protein 3 (LMO3). Circ_0007385 up-regulated LMO3 level by absorbing miR-337-3p in NSCLC cells. LMO3 overexpression largely reversed miR-337-3p overexpression-induced influences in NSCLC cells. Circ_0007385 knockdown significantly restrained the growth of xenograft tumors in vivo. Conclusion. Circ_0007385 promoted the proliferation ability and inhibited the apoptosis of NSCLC cells by binding to miR-337-3p to induce LMO3 expression

Control of sunroof buffeting noise by optimizing the flow field characteristics of a commercial vehicle

When a commercial vehicle is driving with the sunroof open, it is easy for the problem of sunroof buffeting noise to occur. This paper establishes the basis for the design of a commercial vehicle model that solves the problem of sunroof buffeting noise, which is based on computational fluid dynamics (CFD) numerical simulation technology. The large eddy simulation (LES) method was used to analyze the characteristics of the buffeting noise with different speed conditions while the sunroof was open. The simulation results showed that the small vortex generated in the cab forehead merges into a large vortex during the backward movement, and the turbulent vortex causes a resonance response in the cab cavity as the turbulent vortex moves above the sunroof and falls into the cab. Improving the flow field characteristics above the cab can reduce the sunroof buffeting noise. Focusing on the buffeting noise of commercial vehicles, it is proposed that the existing accessories, including sun visors and roof domes, are optimized to deal with the problem of sunroof buffeting noise. The sound pressure level of the sunroof buffeting noise was reduced by 6.7 dB after optimization. At the same time, the local pressure drag of the commercial vehicle was reduced, and the wind resistance coefficient was reduced by 1.55% compared to the original commercial vehicle. These results can be considered as relevant, with high potential applicability, within this field of researchThis work was supported by the National Natural Science Foundation of China (No. 52065013), the Guangxi Youth Science Fund Project (2018GXNSFBA281012), the Innovation-Driven Development Special Fund Project of Guangxi (Guike AA19182004), and the Liuzhou Scientific Research and Planning Development Project (2018AA20301)S

N-(4-hydroxyphenyl) retinamide inhibits migration of renal carcinoma cells and promotes autophagy via MAPK p38 pathway

Purpose: To investigate the effect of N-(4-hydroxyphenyl) retinamide (4HPR) on autophagy and migration of renal carcinoma cells.Methods: Renal cancer cell lines were treated with various concentrations of 4HPR. Proliferation of the cells was studied using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltrazolium bromide (MTT), while apoptosis and cell cycle arrest were determined by flow cytometry.Results: Treatment of RCCs with 30 μM 4HPR caused significant inhibition of viability. In 786-O and OS-RC-2 cell lines, 4HPR reduced colony formation by 39 and 43 %, respectively. In addition, 4HPR increased the percentage of 786-O cells in G1 phase from 58.79 ± 3.43 to 71.68 ± 4.47 % (p < 0.05). It also decreased the percentage of cells in the S-phase from 21.98 ± 2.78 to 09.17 ± 1.43 %, and enhanced the activation of p38 and JNK in 786-O cells at 48 h. Western blot assay showed that the activation of p38 and JNK by 4HPR was inhibited on pre-treatment with SB203580 (inhibitor of p38) and SP600125 (inhibitor of JNK), respectively. Reduction of 786-O cell viability by 4HPR treatment was also significantly inhibited by pre-treatment with sp203580 and sp600125 (p < 0.05). Furthermore, the inhibitors also reversed the effect of 4HPR on the expressions of Bax and Bcl-2 in 786-O cells.Conclusion: These results indicate that 4HPR inhibits the growth of renal cancer cells via activation of MAPK signalling pathway. Thus, 4HPR is a potential drug target for management of renal cancer.Keywords: Retinamide, Renal cancer, Autophagy, MAPK signalling, Cell proliferation, N-terminal kinas

Control of Sunroof Buffeting Noise by Optimizing the Flow Field Characteristics of a Commercial Vehicle

When a commercial vehicle is driving with the sunroof open, it is easy for the problem of sunroof buffeting noise to occur. This paper establishes the basis for the design of a commercial vehicle model that solves the problem of sunroof buffeting noise, which is based on computational fluid dynamics (CFD) numerical simulation technology. The large eddy simulation (LES) method was used to analyze the characteristics of the buffeting noise with different speed conditions while the sunroof was open. The simulation results showed that the small vortex generated in the cab forehead merges into a large vortex during the backward movement, and the turbulent vortex causes a resonance response in the cab cavity as the turbulent vortex moves above the sunroof and falls into the cab. Improving the flow field characteristics above the cab can reduce the sunroof buffeting noise. Focusing on the buffeting noise of commercial vehicles, it is proposed that the existing accessories, including sun visors and roof domes, are optimized to deal with the problem of sunroof buffeting noise. The sound pressure level of the sunroof buffeting noise was reduced by 6.7 dB after optimization. At the same time, the local pressure drag of the commercial vehicle was reduced, and the wind resistance coefficient was reduced by 1.55% compared to the original commercial vehicle. These results can be considered as relevant, with high potential applicability, within this field of research

Exploring the cultivation of innovative talents in mechanical disciplines in the context of the new engineering discipline

With the improvement of China’s economic and technological development level, the concept of “new engineering” has been paid more and more attention. As the main force to promote the development of China’s manufacturing industry, the cultivation of innovative talents of mechanical majors will play a very key role. Based on this, this paper analyzes the current situation of the cultivation of innovative talents in mechanical major. We put forward some effective training strategies, such as the construction of a new talent training model and the establishment of an integrated teaching system

selfreductibilityofmethylammoniumtungstate

A special property of methylammonium tungstate, so-called self-reductibility is studied in the present paper. The researchshows that the tungstate has the characteristics of self-reductibility when it is heated to certain temperature at which it automati-cally decomposes and reduces itself to lower valency tungsten oxides or even metallic tungsten in N 2 . The higher the temperature,the stronger the self-reductibility. A higher temperature is necessary to form metallic tungsten. New processes for the productionof pure oxides WO 2.72 , WO 2 and metallic tungsten W are developed on the basis of the research of self- reductibility of thetungstate

Self-reductibility of Methylammonium Tungstate

A special property of methylammonium tungstate, so-called self-reductibility is studied in the present paper. The researchshows that the tungstate has the characteristics of self-reductibility when it is heated to certain temperature at which it automati-cally decomposes and reduces itself to lower valency tungsten oxides or even metallic tungsten in N 2 . The higher the temperature,the stronger the self-reductibility. A higher temperature is necessary to form metallic tungsten. New processes for the productionof pure oxides WO 2.72 , WO 2 and metallic tungsten W are developed on the basis of the research of self- reductibility of thetungstate

Self-reductibility of Methylammonium Tungstate

A special property of methylammonium tungstate, so-called self-reductibility is studied in the present paper. The researchshows that the tungstate has the characteristics of self-reductibility when it is heated to certain temperature at which it automati-cally decomposes and reduces itself to lower valency tungsten oxides or even metallic tungsten in N 2 . The higher the temperature,the stronger the self-reductibility. A higher temperature is necessary to form metallic tungsten. New processes for the productionof pure oxides WO 2.72 , WO 2 and metallic tungsten W are developed on the basis of the research of self- reductibility of thetungstate

selfreductibilityofmethylammoniumtungstate

A special property of methylammonium tungstate, so-called self-reductibility is studied in the present paper. The researchshows that the tungstate has the characteristics of self-reductibility when it is heated to certain temperature at which it automati-cally decomposes and reduces itself to lower valency tungsten oxides or even metallic tungsten in N 2 . The higher the temperature,the stronger the self-reductibility. A higher temperature is necessary to form metallic tungsten. New processes for the productionof pure oxides WO 2.72 , WO 2 and metallic tungsten W are developed on the basis of the research of self- reductibility of thetungstate

Effect of the microstructure-dependent nonlocality on acoustic black holes

To obtain a well-damped structure, the concept of “acoustic black hole (ABH)” is proposed to trap bending waves at the edge of the wedge shape. However, in the current practice, the remarkable effect of intrinsic lengths is not incorporated. This study aims to reveal the remarkable contribution due to the interplay between intrinsic and extrinsic lengths, which is of fundamental scientific interest. The nonlocal theory is employed to examine the dynamical behaviors of the ABH incorporating the effect of intrinsic lengths. The theoretical model is established for microstructure-dependent power-law plates, and the solution method is developed for analyzing the reflection coefficient of microstructure-dependent power-law plates and bars. It is found that the effect of the microstructure-dependent nonlocality becomes significant and the ABH shows a better absorption of acoustic energy when the nonlocal intrinsic length tends to be lager. Furthermore, the reflection coefficient of traditional works is underestimated. Also, the relationship between the reflection coefficient and wedge truncation length, and frequency is discussed in detail