Práticas formais, informais e de poder sombra no espaço político contemporâneo

The present paper considers various forms and practices of power-related interaction in the political system of modern society. It is noted that the institutional-normative and structural-functional aspects do not reflect the variety of forms and types of communication between public and power. The authors substantiate the methodological position according to which the activity of political subjects should not be considered in traditional theoretical and methodological coordinates, where attention is paid only to "positive phenomena", and power communication is not analyzed from the standpoint of "negative structures", i. e. those aspects which are not recognized as being fit into the dominant type of political and legal activity, into the framework of the emerging social order as a whole. The formal, informal, shadow and other power practices that exist in today's political space are considered in the work from this position.El presente trabajo considera diversas formas y prácticas de interacción relacionada con el poder en el sistema político de la sociedad moderna. Se observa que los aspectos institucional-normativos y estructural-funcionales no reflejan la variedad de formas y tipos de comunicación entre el público y el poder. Los autores corroboran la posición metodológica según la cual la actividad de los sujetos políticos no debe considerarse en las coordenadas teóricas y metodológicas tradicionales, donde la atención se centra únicamente en los "fenómenos positivos" y la comunicación de poder no se analiza desde el punto de vista de las "estructuras negativas", aquellos aspectos que no se reconocen como adecuados para el tipo dominante de actividad política y legal, en el marco del orden social emergente como un todo. Las prácticas formales, informales, de sombra y otras prácticas de poder que existen en el espacio político actual se consideran en el trabajo desde esta posición.O presente trabalho considera diversas formas e práticas de interação relacionadas ao poder no sistema político da sociedade moderna. Observa-se que os aspectos institucionais-normativos e estruturais-funcionais não refletem a variedade de formas e tipos de comunicação entre o público e o poder. Os autores confirmam a posição metodológica que a atividade de sujeitos políticos não deve ser considerada em coordenadas teóricas e metodológicas tradicionais, onde o foco é apenas sobre os "desenvolvimentos positivos" e comunicação de poder não é analisado do ponto de vista das "estruturas negativas", aqueles aspectos que não são reconhecidos como adequados ao tipo dominante de atividade política e legal, no contexto da ordem social emergente como um todo. As práticas formais, informais, sombra e outras práticas de poder que existem no espaço político atual são consideradas no trabalho a partir desta posição

Optimization of hydraulic drive parameters of caterpillar forest loader

The document discusses the methodology for calculating the optimal parameters of a tracked forest loader. Based on the data obtained, a mathematical model of the kinematics of machine drives was compiled when the cargo was released "through itself." As a result of the work, changes in the demand for force on the boom lifting hydraulic cylinder and graphs of the dependence of the forces of the hydraulic cylinders on the angles of rotation of the boom and rocker were built. Based on the results of the work, conclusions were made about the change in the moments changed by the cargo

Low-pass single-chromosome sequencing of human small supernumerary marker chromosomes (sSMCs) and Apodemus B chromosomes.

Supernumerary chromosomes sporadically arise in many eukaryotic species as a result of genomic rearrangements. If present in a substantial part of species population, those are called B chromosomes, or Bs. This is the case for 70 mammalian species, most of which are rodents. In humans, the most common types of extra chromosomes, sSMCs (small supernumerary marker chromosomes), are diagnosed in approximately 1 of 2000 postnatal cases. Due to low frequency in population, human sSMCs are not considered B chromosomes. Genetic content of both B-chromosomes and sSMCs in most cases remains understudied. Here, we apply microdissection of single chromosomes with subsequent low-pass sequencing on Ion Torrent PGM and Illumina MiSeq to identify unique and repetitive DNA sequences present in a single human sSMC and several B chromosomes in mice Apodemus flavicollis and Apodemus peninsulae. The pipeline for sequencing data analysis was made available in Galaxy interface as an addition to previously published command-line version. Human sSMC was attributed to the proximal part of chromosome 15 long arm, and breakpoints leading to its formation were located into satellite DNA arrays. Genetic content of Apodemus B chromosomes was species-specific, and minor alterations were observed in both species. Common features of Bs in these Apodemus species were satellite DNA and ERV enrichment, as well as the presence of the vaccinia-related kinase gene Vrk1. Understanding of the non-essential genome elements content provides important insights into genome evolution in general.This is a post-peer-review, pre-copyedit version of an article published in Chromosoma. The final authenticated version is available online at: [http://dx.doi.org/10.1007/s00412-018-0662-0

Promotion of variant human mammary epithelial cell outgrowth by ionizing radiation: an agent-based model supported by in vitro studies

IntroductionMost human mammary epithelial cells (HMEC) cultured from histologically normal breast tissues enter a senescent state termed stasis after 5 to 20 population doublings. These senescent cells display increased size, contain senescence associated beta-galactosidase activity, and express cyclin-dependent kinase inhibitor, p16INK4A (CDKN2A; p16). However, HMEC grown in a serum-free medium, spontaneously yield, at low frequency, variant (v) HMEC that are capable of long-term growth and are susceptible to genomic instability. We investigated whether ionizing radiation, which increases breast cancer risk in women, affects the rate of vHMEC outgrowth.MethodsPre-stasis HMEC cultures were exposed to 5 to 200 cGy of sparsely (X- or gamma-rays) or densely (1 GeV/amu 56Fe) ionizing radiation. Proliferation (bromodeoxyuridine incorporation), senescence (senescence-associated beta-galactosidase activity), and p16 expression were assayed in subcultured irradiated or unirradiated populations four to six weeks following radiation exposure, when patches of vHMEC became apparent. Long-term growth potential and p16 promoter methylation in subsequent passages were also monitored. Agent-based modeling, incorporating a simple set of rules and underlying assumptions, was used to simulate vHMEC outgrowth and evaluate mechanistic hypotheses.ResultsCultures derived from irradiated cells contained significantly more vHMEC, lacking senescence associated beta-galactosidase or p16 expression, than cultures derived from unirradiated cells. As expected, post-stasis vHMEC cultures derived from both unirradiated and irradiated cells exhibited more extensive methylation of the p16 gene than pre-stasis HMEC cultures. However, the extent of methylation of individual CpG sites in vHMEC samples did not correlate with passage number or treatment. Exposure to sparsely or densely ionizing radiation elicited similar increases in the numbers of vHMEC compared to unirradiated controls. Agent-based modeling indicated that radiation-induced premature senescence of normal HMEC most likely accelerated vHMEC outgrowth through alleviation of spatial constraints. Subsequent experiments using defined co-cultures of vHMEC and senescent cells supported this mechanism.ConclusionsOur studies indicate that ionizing radiation can promote the outgrowth of epigenetically altered cells with pre-malignant potential

Formation of an optimal cement matrix for vibropress products

Modern approaches to the formation of new generation concretes make it possible to replace part of the fine aggregate with finely ground mineral powders, which are by-products of metallurgical and chemical industries, the construction industry, as well as various substandard quartz sands. In the studies carried out, metallurgical slags (MS), broken glass (BG), dust from gas cleaning of metallurgical production (DGCMP), and ground quartz sand (GQS) were used. The aim of the study is to obtain an optimal flow rate of a hydraulic binder by forming an optimal cement matrix. The dosage of finely ground powders varied in the range of 20-49 % of the portland cement consumption. It has been established that the most acceptable finely ground mineral powders in compositions for the production of vibropress products are: quartz ground sand, metallurgical granulated slag of blast furnace production, gas cleaning dust and ground glass. Powders obtained on the basis of glass scrap can be considered as an additional raw material base in the production of materials for road-building purposes. Operational properties such as water absorption and the rate of concrete curing indicate their prospects. However, the economic costs of collecting, sorting glass, obtaining a powder with a specific surface area of 3000 cm2/g are not reasonable at this point in time