Chilling and Freezing Temperature Stress Differently Influence Glucosinolates Content in Brassica oleracea var. acephala

Brassica oleracea var. acephala is known to have a strong tolerance to low temperatures, but the protective mechanisms enabling this tolerance are unknown. Simultaneously, this species is rich in health-promoting compounds such as polyphenols, carotenoids, and glucosinolates. We hypothesize that these metabolites play an important role in the ability to adapt to low temperature stress. To test this hypothesis, we exposed plants to chilling (8 °C) and additional freezing (−8 °C) temperatures under controlled laboratory conditions and determined the levels of proline, chlorophylls, carotenoids, polyphenols, and glucosinolates. Compared with that of the control (21 °C), the chilling and freezing temperatures increased the contents of proline, phenolic acids, and flavonoids. Detailed analysis of individual glucosinolates showed that chilling increased the total amount of aliphatic glucosinolates, while freezing increased the total amount of indolic glucosinolates, including the most abundant indolic glucosinolate glucobrassicin. Our data suggest that glucosinolates are involved in protection against low temperature stress. Individual glucosinolate species are likely to be involved in different protective mechanisms because they show different accumulation trends at chilling and freezing temperatures

Smjernice za akutnu upalu srednjega uha za djecu i odrasle: ambulantno i bolničko liječenje

Akutna upala srednjega uha učestala je bolest čija dijagnostika i liječenje uključuje suradnju liječnika različitih specijalnosti. U ovim smjernicama navedeni su najčešći uzročnici akutne upale uha, dijagnostika upale uha, diferencijalna dijagnoza, te simptomatsko i antibiotsko liječenje. Posebno je naglašen prvi izbor antibiotika, te njegovo doziranje kod djece i odraslih, antibiotsko liječenje nakon neuspjeha liječenja prvim izborom antibiotika, te liječenje recidiva akutne upale srednjega uha. Navedene su indikacije za liječenje akutne upale srednjega uha, te praćenje takvih bolesnika nakon remisije simptoma od strane otorinolaringologa

Smjernice za akutnu upalu srednjega uha za djecu i odrasle: ambulantno i bolničko liječenje

Akutna upala srednjega uha učestala je bolest čija dijagnostika i liječenje uključuje suradnju liječnika različitih specijalnosti. U ovim smjernicama navedeni su najčešći uzročnici akutne upale uha, dijagnostika upale uha, diferencijalna dijagnoza, te simptomatsko i antibiotsko liječenje. Posebno je naglašen prvi izbor antibiotika, te njegovo doziranje kod djece i odraslih, antibiotsko liječenje nakon neuspjeha liječenja prvim izborom antibiotika, te liječenje recidiva akutne upale srednjega uha. Navedene su indikacije za liječenje akutne upale srednjega uha, te praćenje takvih bolesnika nakon remisije simptoma od strane otorinolaringologa

Smjernice za rano prepoznavanje, dijagnostiku i terapiju neurogene orofaringealne disfagije [Guidelines for early detection, diagnostics and therapy of neurogenic oropharyngeal dysphagia]

Guidelines for the early detection, diagnostics and therapy of neurogenic oropharyngeal dysphagia have been made as a result of collaboration of clinicians of different backgrounds who are dealing with patients with neurogenic oropharyngeal dysphagia (NOD). These guidelines have been written by the representatives of the Croatian Society of Clinical Nutrition, Croatian Medical Association, Croatian Neurological Society, Croatian Medical Association, Croatian Society of Gastroenterology and Croatian Society of Nutritionists and Dietitians. The aim of these guidelines is to raise the awareness about NOD that is encountered in acute and chronic neurological diseases, especially in patients with stroke, extrapyramidal diseases, neuromuscular and demyelinisation diseases and dementia. We provide a detailed description of diagnostics of dysphagia, and we recommend the establishment of a multidisciplinary team for dysphagia involving neurologists, internists, speech therapists, dietitians, pharmacists and nurses with special competences for the management of NOD. An educated team member conducts diagnostics and rehabilitation in accordance with the validated tools, classifications and categorizations shown in these guidelines to allow a systematic and consistent treatment. The guidelines also provide detailed algorithms for introducing nutritional support – from the application of modified texture foods, proper hydration to artificial nutrition (enteral and parenteral nutrition)

GUIDELINES FOR EARLY DETECTION, DIAGNOSTICS AND THERAPY OF NEUROGENIC OROPHARYNGEAL DYSPHAGIA

Smjernice za rano prepoznavanje, dijagnostiku i terapiju neurogene orofaringealne disfagije nastale su suradnjom kliničara različitih disciplina koji se bave brojnim aspektima skrbi o bolesnicima s neurogenom orofaringealnom disfagijom (NOD). U izradi smjernica sudjelovali su predstavnici Hrvatskog društva za kliničku prehranu Hrvatskoga liječničkog zbora (HLZ-a), Hrvatskoga neurološkog društva HLZ-a, Hrvatskoga gastroenterološkog društva i Hrvatskog društva nutricionista i dijetetičara. Smjernice imaju cilj povećati svijest o NOD-u koji se javlja kod akutnih i kroničnih neuroloških bolesti, posebice kod moždanog udara, ekstrapiramidnih bolesti, neuromuskularnih i demijelinizacijskih bolesti te demencija. Nadalje, smjernice donose detaljan opis dijagnostike disfagije te preporučuju osnivanje multidisciplinarnog tima za disfagiju u kojem sudjeluju neurolozi, internisti, logopedi, dijetetičari, farmaceuti i medicinske sestre s posebnim kompetencijama na području NOD-a. Educirani član tima provodi dijagnostiku i rehabilitaciju u skladu s provjerenim alatima, klasifikacijama i kategorizacijama prikazanim u ovim smjernicama kako bi se omogućilo sustavno i izjednačeno postupanje. Smjernice donose i detaljne algoritme uvođenja nutritivne potpore – od primjene hrane promijenjene teksture, pravilne hidracije do artificijalne prehrane (enteralne i parenteralne).Guidelines for the early detection, diagnostics and therapy of neurogenic oropharyngeal dysphagia have been made as a result of collaboration of clinicians of different backgrounds who are dealing with patients with neurogenic oropharyngeal dysphagia (NOD). These guidelines have been written by the representatives of the Croatian Society of Clinical Nutrition, Croatian Medical Association, Croatian Neurological Society, Croatian Medical Association, Croatian Society of Gastroenterology and Croatian Society of Nutritionists and Dietitians. The aim of these guidelines is to raise the awareness about NOD that is encountered in acute and chronic neurological diseases, especially in patients with stroke, extrapyramidal diseases, neuromuscular and demyelinisation diseases and dementia. We provide a detailed description of diagnostics of dysphagia, and we recommend the establishment of a multidisciplinary team for dysphagia involving neurologists, internists, speech therapists, dietitians, pharmacists and nurses with special competences for the management of NOD. An educated team member conducts diagnostics and rehabilitation in accordance with the validated tools, classifications and categorizations shown in these guidelines to allow a systematic and consistent treatment. The guidelines also provide detailed algorithms for introducing nutritional support – from the application of modified texture foods, proper hydration to artificial nutrition (enteral and parenteral nutrition)

GUIDELINES FOR EARLY DETECTION, DIAGNOSTICS AND THERAPY OF NEUROGENIC OROPHARYNGEAL DYSPHAGIA

Smjernice za rano prepoznavanje, dijagnostiku i terapiju neurogene orofaringealne disfagije nastale su suradnjom kliničara različitih disciplina koji se bave brojnim aspektima skrbi o bolesnicima s neurogenom orofaringealnom disfagijom (NOD). U izradi smjernica sudjelovali su predstavnici Hrvatskog društva za kliničku prehranu Hrvatskoga liječničkog zbora (HLZ-a), Hrvatskoga neurološkog društva HLZ-a, Hrvatskoga gastroenterološkog društva i Hrvatskog društva nutricionista i dijetetičara. Smjernice imaju cilj povećati svijest o NOD-u koji se javlja kod akutnih i kroničnih neuroloških bolesti, posebice kod moždanog udara, ekstrapiramidnih bolesti, neuromuskularnih i demijelinizacijskih bolesti te demencija. Nadalje, smjernice donose detaljan opis dijagnostike disfagije te preporučuju osnivanje multidisciplinarnog tima za disfagiju u kojem sudjeluju neurolozi, internisti, logopedi, dijetetičari, farmaceuti i medicinske sestre s posebnim kompetencijama na području NOD-a. Educirani član tima provodi dijagnostiku i rehabilitaciju u skladu s provjerenim alatima, klasifikacijama i kategorizacijama prikazanim u ovim smjernicama kako bi se omogućilo sustavno i izjednačeno postupanje. Smjernice donose i detaljne algoritme uvođenja nutritivne potpore – od primjene hrane promijenjene teksture, pravilne hidracije do artificijalne prehrane (enteralne i parenteralne).Guidelines for the early detection, diagnostics and therapy of neurogenic oropharyngeal dysphagia have been made as a result of collaboration of clinicians of different backgrounds who are dealing with patients with neurogenic oropharyngeal dysphagia (NOD). These guidelines have been written by the representatives of the Croatian Society of Clinical Nutrition, Croatian Medical Association, Croatian Neurological Society, Croatian Medical Association, Croatian Society of Gastroenterology and Croatian Society of Nutritionists and Dietitians. The aim of these guidelines is to raise the awareness about NOD that is encountered in acute and chronic neurological diseases, especially in patients with stroke, extrapyramidal diseases, neuromuscular and demyelinisation diseases and dementia. We provide a detailed description of diagnostics of dysphagia, and we recommend the establishment of a multidisciplinary team for dysphagia involving neurologists, internists, speech therapists, dietitians, pharmacists and nurses with special competences for the management of NOD. An educated team member conducts diagnostics and rehabilitation in accordance with the validated tools, classifications and categorizations shown in these guidelines to allow a systematic and consistent treatment. The guidelines also provide detailed algorithms for introducing nutritional support – from the application of modified texture foods, proper hydration to artificial nutrition (enteral and parenteral nutrition)

The \u3cem\u3eChlamydomonas\u3c/em\u3e Genome Reveals the Evolution of Key Animal and Plant Functions

Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It is a model system for studying chloroplast-based photosynthesis, as well as the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited from the common ancestor of plants and animals, but lost in land plants. We sequenced the ∼120-megabase nuclear genome of Chlamydomonas and performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated with the function and biogenesis of chloroplasts or eukaryotic flagella. Analyses of the Chlamydomonas genome advance our understanding of the ancestral eukaryotic cell, reveal previously unknown genes associated with photosynthetic and flagellar functions, and establish links between ciliopathy and the composition and function of flagella

UPDATE ON GUIDELINES FOR ACUTE STROKE TREATMENT BY THE CROATIAN SOCIETY FOR NEUROVASCULAR DISORDERS OF THE CROATIAN MEDICAL ASSOCIATION AND THE CROATIAN STROKE SOCIETY

Cilj ovih smjernica bio je predati najnovije, sistematizirane spoznaje kliničarima koji se bave zbrinjavanjem odraslih bolesnika s akutnim ishemijskim moždanim udarom. Preveli smo Američke smjernice za liječenje akutnog ishemijskog moždanog udara, koje su izdane 2018. godine od American Heart Association (AHA) i American Stroke Association (ASA) s ciljem prilagodbe hrvatskom zakonodavstvu i zdravstvenom sustavu. Članovi radne skupine Hrvatskog društva za neurovaskularne poremećaje Hrvatskog liječničkog zbora i Hrvatskog društva za moždani udar usmjerili su posebnu pozornost na pregled dosadašnjih spoznaja i prilagodili ih aktualnom stanju u nacionalnom zdravstvenom sustavu kako bi se postiglo optimalno zbrinjavanje bolesnika s akutnim ishemijskim moždanim udarom u Hrvatskoj. Ove smjernice obuhvaćaju prehospitalnu skrb, hitnu evaluaciju te liječenje intravenskom i intraarterijskom terapijom uključujući mjere sekundarne prevencije prigodom inicijalne hospitalizacije u odraslih pacijenata s akutnim moždanim udarom.The aim of these guidelines is to provide an update and comprehensive review of the recent literature for clinicians treating adult patients with acute stroke. We translated American guidelines for the acute ischemic stroke treatment, published in 2018 by the American Heart Association and American Stroke Association, and adapted these guidelines to the Croatian legislation, health insurance policy and standards of health care in Croatia. Members of the Croatian Society for Neurovascular Disorders of the Croatian Medical Association and the Croatian Stroke Society writing group evaluated and adjusted American guidelines to specifi cities of the Croatian health care system in order to improve the quality of stroke management in Croatia. These guidelines include pre-hospital care, urgent evaluation and treatment with intravenous and intra-arterial therapy, as well as the secondary prevention measures during initial hospitalization in adult acute stroke patients

UPDATE ON GUIDELINES FOR ACUTE STROKE TREATMENT BY THE CROATIAN SOCIETY FOR NEUROVASCULAR DISORDERS OF THE CROATIAN MEDICAL ASSOCIATION AND THE CROATIAN STROKE SOCIETY

Cilj ovih smjernica bio je predati najnovije, sistematizirane spoznaje kliničarima koji se bave zbrinjavanjem odraslih bolesnika s akutnim ishemijskim moždanim udarom. Preveli smo Američke smjernice za liječenje akutnog ishemijskog moždanog udara, koje su izdane 2018. godine od American Heart Association (AHA) i American Stroke Association (ASA) s ciljem prilagodbe hrvatskom zakonodavstvu i zdravstvenom sustavu. Članovi radne skupine Hrvatskog društva za neurovaskularne poremećaje Hrvatskog liječničkog zbora i Hrvatskog društva za moždani udar usmjerili su posebnu pozornost na pregled dosadašnjih spoznaja i prilagodili ih aktualnom stanju u nacionalnom zdravstvenom sustavu kako bi se postiglo optimalno zbrinjavanje bolesnika s akutnim ishemijskim moždanim udarom u Hrvatskoj. Ove smjernice obuhvaćaju prehospitalnu skrb, hitnu evaluaciju te liječenje intravenskom i intraarterijskom terapijom uključujući mjere sekundarne prevencije prigodom inicijalne hospitalizacije u odraslih pacijenata s akutnim moždanim udarom.The aim of these guidelines is to provide an update and comprehensive review of the recent literature for clinicians treating adult patients with acute stroke. We translated American guidelines for the acute ischemic stroke treatment, published in 2018 by the American Heart Association and American Stroke Association, and adapted these guidelines to the Croatian legislation, health insurance policy and standards of health care in Croatia. Members of the Croatian Society for Neurovascular Disorders of the Croatian Medical Association and the Croatian Stroke Society writing group evaluated and adjusted American guidelines to specifi cities of the Croatian health care system in order to improve the quality of stroke management in Croatia. These guidelines include pre-hospital care, urgent evaluation and treatment with intravenous and intra-arterial therapy, as well as the secondary prevention measures during initial hospitalization in adult acute stroke patients

Nuclear localised more sulphur accumulation1 epigenetically regulates sulphur homeostasis in Arabidopsis thaliana

Sulphur (S) is an essential element for all living organisms. The uptake, assimilation and metabolism of S in plants are well studied. However, the regulation of S homeostasis remains largely unknown. Here, we report on the identification and characterisation of the more sulphur accumulation1 (msa1-1) mutant. The MSA1 protein is localized to the nucleus and is required for both S adenosylmethionine (SAM) production and DNA methylation. Loss of function of the nuclear localised MSA1 leads to a reduction in SAM in roots and a strong S-deficiency response even at ample S supply, causing an over- accumulation of sulphate, sulphite, cysteine and glutathione. Supplementation with SAM suppresses this high S phenotype. Furthermore, mutation of MSA1 affects genome-wide DNA methylation, including the methylation of S-deficiency responsive genes. Elevated S accumulation in msa1-1 requires the increased expression of the sulphate transporter genes SULTR1;1 and SULTR1;2 which are also differentially methylated in msa1-1. Our results suggest a novel function for MSA1 in the nucleus in regulating SAM biosynthesis and maintaining S homeostasis epigenetically via DNA methylation