Pelaksanaan Pemberantasan Sarang Nyamuk Demam Berdarah Dengue Berbasis Perilaku Masyarakat di Kalipancur, Semarang

Central Java Public Health Authorities have recorded that Semarang has the highest Incidence Rate (IR) of Dengue Hemorrhagic Fever (DHF) since 2009 to 2011. Ngaliyan district, part of Semarang region have suffered from DHF endemic. This research is conducted in Kalipancur, part of Ngaliyan district that aims to determine the behavioral domain of implementation of DHF mosquito nest eradication (3MPlus) based on the knowledge, attitudes and actions following by characteristic of thecommunity.This analytic observational research with cross sectional study involved 107 respondents. Primary data was collected through interviews using questionnaire related to knowledge, attitudes, actions and implementation of 3M Plus. Data was analyzed with chi square test, Confident Interval 95%.Results shows that 60.7% of community have low level of knowledge and 74.8% of them taking less actions regarding the implementation of 3M Plus. Nevertheless, 72% of respondents had a good attitudes. Both of knowledge (p=0,08) and actions (p=0,104) did not have a significant impact to the implementation of 3 M Plus. On the contrary, respondents attitudes (p=0,002) were found to be significant factor related to the implementation of 3M Plus. Therefore, provision of health information is needed to improve knowledge and actions of mosquito nest eradication of DHF

Optimasi Dan Validasi Metode Penetapan Kadar Siprofloksasin Dalam Media Mueller Hinton Broth Menggunakan Hplc (High Performance Liquid Chromatography)

Model kinetika in vitro telah dikembangkan untuk menggambarkan simulasi farmakokinetika antibiotika sesuai dengan profil farmakokinetika pada tubuh manusia. Untuk melakukan penelitian model kinetika in vitro, salah satu faktor penting untuk dianalisis adalah kadar obat dalam media bakteri yang disesuaikan dengan kadar obat dalam tubuh manusia. Penelitian ini bertujuan untuk melakukan analisis optimasi dan validasi penetapan kadar antibiotik siprofloksasin dalam media Mueller Hinton Broth (MHB), menggunakan kromatografi cair kinerja tinggi (HPLC) UV Vis pada panjang gelombang 275 nm. Pemisahan kromatografi dilakukan menggunakan kolom C18 (250 x 4,6 mm; 4,6 µm; Knauer Jerman). Fase gerak isokratik terdiri dari 0,02 M buffer natrium dihidrogen fosfat pH ± 3,0 dan asetonitril (65:35, v/v). Fase gerak mengandung 5 mM trietilamin sebagai agen pasangan ion. Laju fase gerak konstan 0,8 mL/menit, pada suhu kolom 42°C, dan tekanan kolom berkisar 183 – 198 kgf. Metode yang digunakan selektif dapat memisahkan puncak area kromatogram dengan media MHB. Waktu retensi berada pada 3,74 menit (SD 0,04; CV 1,06%). Metode ini valid dan linear pada rentang konsentrasi 0,1 – 10 µg/mL (r2 = 0,992). Sensitivitas ditunjukkan dengan nilai LOD dan LLOQ sebesar 1,24 µg/mL dan 4,12 µg/mL. Stabilitas sampel yang diukur pada penyimpanan 7 hari suhu 2 – 8°C menunjukkan nilai perolehan kembali hasil simpan sebesar 93,80%

Studi Korelasi Anemia pada Ibu Hamil dengan Kejadian Perdarahan Post Partum pada Persalinan Spontan

Latar Belakang :Jumlah kematian ibu di Indonesia masih banyak terjadi. Penyebab kematian ibu yang paling dominan adalah perdarahanpost partum. Perdarahan post partum juga memiliki beberapa faktor predisposisi. Penelitian ini bertujuan untuk mengetahui pengaruh darifaktor predisposisi yaitu usia, paritas, dan anemia terhadap terjadinya perdarahan post partum pada persalinan spontan di RSUD Tugurejotahun 2011.Metode : Penelitian ini merupakan studi observasional dengan desain cross sectional yang dianalisis dengan uji statistik chi square yangmeliputi analisis univariat, analisis bivariat dan analisis multivariat terhadap variabel usia, paritas, anemia, terhadap kejadian perdarahan postpartum pada persalinan spontan. Sampel dalam penelitian ini sebanyak 40 ibu. Pengambilan sampel dilakukan dengan teknik randomsampling dengan mengambil data dari rekam medis.Hasil : Hasil analisis bivariat dari 40 ibu dengan persalinan spontan, variabel usia (p = 0,123) dan variabel paritas (p = 0,073) menunjukkanbahwa tidak terdapat pengaruh antara usia dan paritas dengan kejadian perdarahan post partum. Sedangkan variabel anemia (p = 0,009)menunjukkan bahwa terdapat pengaruh antara anemia dengan kejadian perdarahan post partum. Hasil analisis multivariat menunjukan bahwavariabel anemia (OR = 6,172; p = 0,015; 95% CI = 1,414-26,940 merupakan faktor yang berpengaruh terhadap terjadinya perdarahan postpartum, dan ibu yang menderita anemia memiliki kemungkinan 6 kali lebih besar mengalami perdarahan post partum dibandingkan denganibu yang tidak menderita anemia.Simpulan : Ada pengaruh antara anemia dengan kejadian perdarahan post partum dan tidak ada pengaruh antara usia dan paritas dengankejadian perdarahan post partum. Kata kunci : Usia, Paritas, Anemia, Perdarahan post partu

Hasil Cek Similarity" When plasma jet is effective for chronic wound bacteria inactivation, is it also effective for wound healing?"

Purpose: This investigation aimed to compare the effectiveness of two styles of plasma jet treatment (i.e., contact and non-contact styles) for two biological materials, namely, wound related bacteria and acute wounds. Method: An atmospheric plasma jet operated at a frequency of 18.32 kHz and high AC voltage with a peak-topeak voltage of 9.58 kV and a current of 55.2 mA was applied. Argon gas was used as the carries gas of plasma jet generation and was fixed at a flow rate of 1 standard liters per minute (slm).Two biological materials (i.e., wound related bacteria and acute wound) were applied as experimental objects. The sample groups were based on the two styles of plasma jet treatment: contact and non-contact styles. Microbial inhibition zone calculation and macroscopic and histological observations were also performed. Results: This investigation emphasized that the contact and non-contact styles of plasma jet treatment had significantly different effects for wounds and wound-related chronic bacteria. On the one hand, the contact style was visually attractive and more effective for inactivate bacteria. On the other hand, it caused negative effects, such as damaging normal tissue, significantly impeding wound healing and impeding the growing of new epithelial tissue. The non-contact style, however, was less effective at inactivating bacteria; however, it could accelerate wound healing. Conclusion: In the context of wound healing, the non-contact style of plasma jet treatment may be better than the contact style of plasma jet treatment

Sexual Dimorphism of the First Lumbar Vertebra in the Malaysian Population.

Sex determination is one of the main steps in the identification of human skeletal remains. The vertebrae are weight-bearing structures in the human body that may provide variety of information from an individual.  The aim of this study is to assess the sexual dimorphism of the first lumbar (L1) vertebrae using three-dimensional (3D) computed tomography (CT) imaging to develop population-specific equations for sex identification in the Malay population. Thirteen linear measurements of the first lumbar (L1) vertebrae were taken from 50 males and 50 females' patients in the Radiology Department of Universiti Kebangsaan Malaysia Medical Centre, using images of the Computed Tomography (CT) scan. Independent T-test and discriminant function analysis (DFA) were performed for analysis. By using independent T-test analysis, there were eight measurements showed statistically significant difference between men and women (p<0.001). Using stepwise method of discriminant analysis showed three measurements predicted sex with the accuracy 93.0% : (a) lower end-plate width (EPWI), (b) lower end-plate depth (EPDI), and posterior height of the vertebral body (VBHp). This study provides discriminant equation for forensic identification of sex from the first lumbar vertebrae among Malaysia population with the accuracy 93.0%