Raman-Active Resonance Modes, Overtones, and Anharmonicity in NaCl:Cu\u3csup\u3e+\u3c/sup\u3e

The existence of an impurity-activated Eg resonance mode in NaCl:Cu+ has been suggested by several previous experiments. Raman data presented here reveal this resonance directly and also reveal the three components of the first overtone of the 23.5-cm-1 infrared resonance mode. The frequencies of the Eg resonance and the Eg component of the overtone are shifted as a result of a strong anharmonic coupling. Their line shapes and strengths are considerably altered by an interference between the Raman amplitudes. A reasonable fit to the data has been obtained using a simple theory

Structure-function analysis of the curli accessory protein CsgE defines surfaces essential for coordinating amyloid fiber formation

Curli amyloid fibers are produced as part of the extracellular biofilm matrix and are composed primarily of the major structural subunit CsgA. The CsgE chaperone facilitates the secretion of CsgA through CsgG by forming a cap at the base of the nonameric CsgG outer membrane pore. We elucidated a series of finely tuned nonpolar and charge-charge interactions that facilitate the oligomerization of CsgE and its ability to transport unfolded CsgA to CsgG for translocation. CsgE oligomerization in vitro is temperature dependent and is disrupted by mutations in the W48 and F79 residues. Using nuclear magnetic resonance (NMR), we identified two regions of CsgE involved in the CsgE-CsgA interaction: a head comprising a positively charged patch centered around R47 and a stem comprising a negatively charged patch containing E31 and E85. Negatively charged residues in the intrinsically disordered N- and C-terminal “tails” were not implicated in this interaction. Head and stem residues were mutated and interrogated using in vivo measurements of curli production and in vitro amyloid polymerization assays. The R47 head residue of CsgE is required for stabilization of CsgA- and CsgE-mediated curli fiber formation. Mutation of the E31 and E85 stem residues to positively charged side chains decreased CsgE-mediated curli fiber formation but increased CsgE-mediated stabilization of CsgA. No single-amino-acid substitutions in the head, stem, or tail regions affected the ability of CsgE to cap the CsgG pore as determined by a bile salt sensitivity assay. These mechanistic insights into the directed assembly of functional amyloids in extracellular biofilms elucidate possible targets for biofilm-associated bacterial infections.Curli represent a class of functional amyloid fibers produced by Escherichia coli and other Gram-negative bacteria that serve as protein scaffolds in the extracellular biofilm matrix. Despite the lack of sequence conservation among different amyloidogenic proteins, the structural and biophysical properties of functional amyloids such as curli closely resemble those of amyloids associated with several common neurodegenerative diseases. These parallels are underscored by the observation that certain proteins and chemicals can prevent amyloid formation by the major curli subunit CsgA and by alpha-synuclein, the amyloid-forming protein found in Lewy bodies during Parkinson’s disease. CsgA subunits are targeted to the CsgG outer membrane pore by CsgE prior to secretion and assembly into fibers. Here, we use biophysical, biochemical, and genetic approaches to elucidate a mechanistic understanding of CsgE function in curli biogenesis

Role of copper efflux in pneumococcal pathogenesis and resistance to macrophage-mediated immune clearance

In bacteria, the intracellular levels of metals are mediated by tightly controlled acquisition and efflux systems. This is particularly true of copper, a trace element that is universally toxic in excess. During infection, the toxic properties of copper are exploited by the mammalian host to facilitate bacterial clearance. To better understand the role of copper during infection, we characterized the contribution of the cop operon to copper homeostasis and virulence in Streptococcus pneumoniae. Deletion of either the exporter, encoded by copA, or the chaperone, encoded by cupA, led to hypersensitivity to copper stress. We further demonstrated that loss of the copper exporter encoded by copA led to decreased virulence in pulmonary, intraperitoneal, and intravenous models of infection. Deletion of copA resulted in enhanced macrophage-mediated bacterial clearance in vitro. The attenuation phenotype of the copA mutant in the lung was found to be dependent on pulmonary macrophages, underscoring the importance of copper efflux in evading immune defenses. Overall, these data provide insight into the role of the cop operon in pneumococcal pathogenesis

EC98-103 Nebraska Fall-Sown Small Grain Variety Tests, 1998

This circular is a progress report of variety trials conducted by personnel of the Agronomy Department and the South Central, West Central and Panhandle Research and Extension Centers and their associated agricultural laboratories. Conduct of experiments and publication of results is a joint effort of the Agricultural Research Division and the Cooperative Extension Service

EC03-101 Nebraska Seed Guide, 2004

This circular is a progress report of corn hybrid performance tests conducted by the Agronomy/Horticulture Department and the Northeast, South Central, West Central and Panhandle Research and Extension Centers of Nebraska and University of Wyoming at Torrington. Conduct of experiments and publication of results is a joint effort of the Agricultural Research Division and the Cooperative Extension Service

EC03-101 Nebraska Seed Guide, 2004

This circular is a progress report of corn hybrid performance tests conducted by the Agronomy/Horticulture Department and the Northeast, South Central, West Central and Panhandle Research and Extension Centers of Nebraska and University of Wyoming at Torrington. Conduct of experiments and publication of results is a joint effort of the Agricultural Research Division and the Cooperative Extension Service

Antimicrobial susceptibility monitoring of Mycoplasma hyopneumoniae isolated from seven European countries during 2015-2016

Mycoplasma hyopneumoniae is the causative agent of porcine enzootic pneumonia, a chronic respiratory disease, causing significant economic losses. Results from the 2015-2016 MycoPath pan-European antimicrobial susceptibility monitoring survey of M. hyopneumoniae are presented. In total, 147 M. hyopneumoniae porcine isolates from Belgium, France, Germany, Great Britain, Hungary, Italy, and Spain were tested. One isolate per farm was retained from pigs that had not been recently treated with antimicrobial agents. The minimal inhibitory concentration (MIC) of 13 antimicrobial agents was determined in a central laboratory using a broth microdilution method, with Friis Medium, incubated at 35 +/- 1 degrees C for 5-12 days. M. hyopneumoniae NCTC 10110 was used as Quality Control. MIC50/MIC90 (mg/L) values were: enrofloxacin 0.06/1; marbofloxacin 0.06/2; spiramycin 0.06/0.25; tulathromycin <= 0.001/0.004; gamithromycin 0.06/0.5; tylosin 0.016/0.06; tilmicosin 0.06/0.5; florfenicol 0.5/1; doxycycline 0.25/1; oxytetracycline 0.25/2; lincomycin 0.06/0.25; tiamulin 0.016/0.06 and valnemulin <= 0.001/0.004. Compared with the data from 2010 to 2012 MycoPath study (50 isolates), MIC50/90 results were similar and the majority were within +/- two dilution steps, except for the MIC50 of oxytetracycline which is more than two dilution steps higher in the present study. Between-country comparisons show some differences in the MIC values for the fluoroquinolones, tulathromycin and tylosin, but the limited sample size per country precludes performing meaningful country comparisons for several countries. Standardized laboratory methods and interpretive criteria for MIC testing of veterinary mycoplasmas are clearly needed; there are currently no clinical breakpoints available to facilitate data interpretation and correlation of MICs with in vivo efficacy

From 10 Kelvin to 10 TeraKelvin: Insights on the Interaction Between Cosmic Rays and Gas in Starbursts

Recent work has both illuminated and mystified our attempts to understand cosmic rays (CRs) in starburst galaxies. I discuss my new research exploring how CRs interact with the ISM in starbursts. Molecular clouds provide targets for CR protons to produce pionic gamma rays and ionization, but those same losses may shield the cloud interiors. In the densest molecular clouds, gamma rays and Al-26 decay can provide ionization, at rates up to those in Milky Way molecular clouds. I then consider the free-free absorption of low frequency radio emission from starbursts, which I argue arises from many small, discrete H II regions rather than from a "uniform slab" of ionized gas, whereas synchrotron emission arises outside them. Finally, noting that the hot superwind gas phase fills most of the volume of starbursts, I suggest that it has turbulent-driven magnetic fields powered by supernovae, and that this phase is where most synchrotron emission arises. I show how such a scenario could explain the far-infrared radio correlation, in context of my previous work. A big issue is that radio and gamma-ray observations imply CRs also must interact with dense gas. Understanding how this happens requires a more advanced understanding of turbulence and CR propagation.Comment: Conference proceedings for "Cosmic-ray induced phenomenology in star-forming environments: Proceedings of the 2nd Session of the Sant Cugat Forum of Astrophysics" (April 16-19, 2012). 16 pages, 5 figure

Potential effects of oilseed rape expressing oryzacystatin-1 (OC-1) and of purified insecticidal proteins on larvae of the solitary bee Osmia bicornis

Despite their importance as pollinators in crops and wild plants, solitary bees have not previously been included in non-target testing of insect-resistant transgenic crop plants. Larvae of many solitary bees feed almost exclusively on pollen and thus could be highly exposed to transgene products expressed in the pollen. The potential effects of pollen from oilseed rape expressing the cysteine protease inhibitor oryzacystatin-1 (OC-1) were investigated on larvae of the solitary bee Osmia bicornis (= O. rufa). Furthermore, recombinant OC-1 (rOC-1), the Bt toxin Cry1Ab and the snowdrop lectin Galanthus nivalis agglutinin (GNA) were evaluated for effects on the life history parameters of this important pollinator. Pollen provisions from transgenic OC-1 oilseed rape did not affect overall development. Similarly, high doses of rOC-1 and Cry1Ab as well as a low dose of GNA failed to cause any significant effects. However, a high dose of GNA (0.1%) in the larval diet resulted in significantly increased development time and reduced efficiency in conversion of pollen food into larval body weight. Our results suggest that OC-1 and Cry1Ab expressing transgenic crops would pose a negligible risk for O. bicornis larvae, whereas GNA expressing plants could cause detrimental effects, but only if bees were exposed to high levels of the protein. The described bioassay with bee brood is not only suitable for early tier non-target tests of transgenic plants, but also has broader applicability to other crop protection products