Parceria de serviços de Saúde Pública e privados com a academia, no combate à covid-19: relato de experiência em Tubarão, Santa Catarina

This article presents a report on the integration experience of health stakeholders, including public and private health systems, a university and authorities for the surveillance and control of the COVID-19 epidemic, in the municipality of Tubarão, Santa Catarina, Brazil. The city has a large flow of people from different parts of the country, and was one of the first municipalities in the state of Santa Catarina to report cases of community transmission by SARS-CoV-2. The measures adopted included the implementation of the COVID-19 Monitoring Committee, the Health Center for Municipal Emergency Operations, and the Disease Contingency Plan. After 100 days of pandemic, 5,979 cases were reported, 431 (7.2%) confirmed, of which five (1.2%) died. Early decisions, such as the immediate suspension of business activities and crowding events, have reduced the spread of the virus. The established partnership provided innovation and subsidized public management in decision-making based upon scientific evidence.Apresenta-se o relato de experiência da integração entre serviços de saúde públicos e privados, gestores e universidade, para a vigilância e controle da epidemia de covid-19 em Tubarão, SC, Brasil. A cidade, universitária, cenário de grande fluxo de pessoas de diferentes locais do país, foi um dos primeiros municípios do estado catarinense com transmissão comunitária do SARS-CoV-2. São detalhadas as medidas adotadas com a criação do Comitê de Monitoramento da Covid-19, do Centro de Operações de Emergências Municipais em Saúde, e do Plano de Contingência da Doença. Passados 100 dias de pandemia, foram 5.979 casos notificados e 431 (7,2%) confirmados, dos quais 5 (1,2%) idos a óbito. Decisões precoces – suspensão imediata das atividades de comércio e eventos com aglomeração – podem ter limitado a propagação do vírus. As parcerias estabelecidas trazem inovação e subsidiam a gestão pública nas tomadas de decisão pautadas em evidências científicas

Different methods of cell quantification can lead to different results : a comparison of digital methods using a pilot study of dendritic cells in HIV-positive patients

Although new digital pathology tools have improved the positive cell quantification, there is a heterogeneity of the quantification methods in the literature. The aim of this study was to evaluate and propose a novel dendritic cells quantification method in squamous cell carcinoma comparing it with a conventional quantification method. Twenty-six squamous cell carcinomas HIV-positive cases affecting the oropharynx, lips and oral cavity were selected. Immunohistochemistry for CD1a, CD83, and CD207 was performed. The immunohistochemical stains were evaluated by automated examination using a positive pixel count algorithm. A conventional quantification method (unspecific area method; UA) and a novel method (specific area method; SA) were performed obtaining the corresponding density of positive dendritic cells for the intratumoral and peritumoral regions. The Mann-Whitney U test was used to verify the influence of the quantification methods on the positive cell counting according to the evaluated regions. Data were subjected to the ANOVA and Student?s t-test to verify the influence of the tumour location, stage, histological grade, and amount of inflammation on the dendritic cells density counting. The cell quantification method affected the dendritic cells counting independently of the evaluated region (P-value <0.05). Significant differences between methods were also observed according to the tumour features evaluations. The positive cell quantification method influences the dendritic cells density results. Unlike the conventional method (UA method), the novel SA method avoids non-target areas included in the hotspots improving the reliability and reproducibility of the density cell quantification

New Delhi metallo-beta-lactamase-1-producing Acinetobacter spp. infection: report of a survivor

Abstract: New Delhi metallo-beta-lactamase-1 (NDM-1) is a bacterial enzyme that renders the bacteria resistant to a variety of beta-lactam antibiotics. A 20-year-old man was hospitalized several times for surgical treatment and complications caused by a right-sided vestibular schwannoma. Although the patient acquired several multidrug-resistant infections, this study focuses on the NDM-1-producing Acinetobacter spp. infection. As it was resistant to all antimicrobials tested, the medical team developed a 20-day regimen of 750mg/day metronidazole, 2,000,000IU/day polymyxin B, and 100mg/day tigecycline. The treatment was effective, and the patient recovered and was discharged from the hospital

New Delhi metallo-beta-lactamase-1-producing Acinetobacter spp. infecions: report of a survivor

New Delhi metallo-beta-lactamase-1 (NDM-1) is a bacterial enzyme that renders the bacteria resistant to a variety of beta-lactam antibiotics. A 20-year-old man was hospitalized several times for surgical treatment and complications caused by a right-sided vestibular schwannoma. Although the patient acquired several multidrug-resistant infections, this study focuses on the NDM- 1-producing Acinetobacter spp. infection. As it was resistant to all antimicrobials tested, the medical team developed a 20-day regimen of 750mg/day metronidazole, 2,000,000IU/day polymyxin B, and 100mg/day tigecycline. The treatment was effective, and the patient recovered and was discharged from the hospital.Não há