Utilisation of microvertebrates in biostratigraphy and chemostratigraphy

The utility of marine microvertebrate fossils for bio- and chemostratigraphy and subsequent correlation were tested on the exceptionally preserved Devonian reef complexes of the Canning Basin, Western Australia. Microvertebrates provided refined age ranges for restricted depositional environments unable to be dated using conodonts or ammonoids. In addition, the O-isotope signatures recovered from hypermineralised tissues within microvertebrate fossils enabled the reconstruction of palaeoenvironmental conditions, in addition to providing the basis for chemical stratigraphy

Upper Devonian microvertebrates from the Canning Basin, Western Australia

A diverse microvertebrate fauna is described from the Virgin Hills and Napier formations, Bugle Gap Limestone Canning Basin, Western Australia. Measured sections at horse Spring and Casey Falls (Virgin Hills Formation) and South Oscar Range (Napier Formation) comprise proximal to distal slope carbonates ranging in age from the Late Devonian Frasnian to middle Famennian. A total of 18 chondrichthyan taxa are identified based on teeth, including the first record of Thrinacodus tranquillus, Cladoides wildungensis, Protacrodus serra and Lissodus lusavorichi from the Canning Basin. A new species, Diademodus dominicus sp. nov. is also described and provided the first record of this genus outside of Laurussia. In addition, the upper range of Australolepis seddoni has been extended to Late Devonian conodont Zone 11, making it the youngest known occurrence for this species. The Virgin Hills and Napier formations microvertebrate faunas show close affinities to faunas recovered from other areas of Gondwana, including eastern Australia, Iran, Morocco and South China, which is consistent with known conodont and trilobite faunas of the same age

Upper Kellwasser carbon isotope excursion pre-dates the F–F boundary in the Upper Devonian Lennard shelf carbonate system, Canning Basin, Western Australia

Here we report four high-resolution carbon isotope records in addition to trace element data for the Frasnian–Famennian (F–F) boundary interval in the Lennard Shelf carbonate system of the Canning Basin, Western Australia. This region lacks the characteristic black shale horizons associated with the global Late Devonian Kellwasser extinction events, yet still exhibits a trend in carbon isotope character similar to what has been reported from elsewhere in the world (two positive δ13C excursions with ~3–4‰ amplitudes). Enrichments in select trace element ratios suggest that both excursions are related to periods of oxygen deprivation and perhaps increased biological productivity. Given the continuous and stratigraphically expanded nature of Lennard Shelf sections, together with high-density sampling constrained by both conodont biostratigraphy and magnetostratigraphy, we observe that the Upper Kellwasser isotope excursion (maximum δ13C values) and associated trace element enrichments occur distinctly lower than the F–F boundary level. These results have implications for the paleoenvironmental conditions leading up to the Late Devonian Mass Extinction in terms of ocean chemistry and circulation patterns. This data set allows for a rare, detailed look at the temporal relationship between the Kellwasser events and the F–F boundary and constrains the pattern of carbon isotope perturbations at the intra-zonal scale

Integrated stratigraphic correlation of Upper Devonian platform-to-basin carbonate sequences, Lennard Shelf, Canning Basin, Western Australia: advances in carbonate margin-to-slope sequence stratigraphy and stacking patterns

High-resolution, time-significant correlations are integral to meaningful stratigraphic frameworks in depositional systems, but may be difficult to achieve using traditional sequence stratigraphic or biostratigraphic approaches alone, particularly in geologically complex settings. In steep, reefal carbonate margin-to-slope systems, such correlations are essential to unravel shelf-to-basin transitions, characterize strike variability, and develop predictive sequence stratigraphic models – concepts which are currently poorly understood in these heterogeneous settings. The Canning Basin Chronostratigraphy Project (CBCP) integrates multiple independent datasets (including biostratigraphy, magnetostratigraphy, stable isotope chemostratigraphy, and sequence stratigraphy) extracted from Upper Devonian (Frasnian and Famennian) reefal platform exposures along the Lennard Shelf, Canning Basin, Western Australia. These were used to generate a well-constrained stratigraphic framework and shelf-to-basin composite reconstruction of the carbonate system. The resultant integrated framework allows for unprecedented analysis of carbonate margin-to-slope heterogeneity, depositional architecture, and sequence stratigraphy along the Lennard Shelf. Systems tract architecture, facies partitioning, and stacking patterns of margin to lower-slope environments were assessed for six composite-scale sequences that form part of a transgressive-to-regressive supersequence and span the Frasnian-Famennian (F-F) biotic crisis. Variations are apparent in margin styles, foreslope facies proportions, dominant resedimentation processes, downslope contributing sediment factories, and vertical rock successions, related to hierarchical accommodation signals and ecological changes associated with F-F boundary. We present these results in the form of carbonate margin-to-basin sequence stratigraphic models and associations that link seismic-scale architecture to fine-scale facies heterogeneity. These models provide a predictive foundation for characterization of steep-sided flanks of reefal carbonate platform systems that is useful for both industry and academia. This study emphasizes the utility of an integrated stratigraphic approach and the insights gained from better-constrained facies and stratal architecture analysis; insights that were not achievable with traditional sequence stratigraphic or biostratigraphic techniques alone

Impaired mitochondrial complex I function as a candidate driver in the biological stress response and a concomitant stress-induced brain metabolic reprogramming in male mice

Mitochondria play a critical role in bioenergetics, enabling stress adaptation, and therefore, are central in biological stress responses and stress-related complex psychopathologies. To investigate the effect of mitochondrial dysfunction on the stress response and the impact on various biological domains linked to the pathobiology of depression, a novel mouse model was created. These mice harbor a gene trap in the first intron of the Ndufs4 gene (Ndufs4GT/GT mice), encoding the NDUFS4 protein, a structural component of complex I (CI), the first enzyme of the mitochondrial electron transport chain. We performed a comprehensive behavioral screening with a broad range of behavioral, physiological, and endocrine markers, high-resolution ex vivo brain imaging, brain immunohistochemistry, and multi-platform targeted mass spectrometry-based metabolomics. Ndufs4GT/GT mice presented with a 25% reduction of CI activity in the hippocampus, resulting in a relatively mild phenotype of reduced body weight, increased physical activity, decreased neurogenesis and neuroinflammation compared to WT littermates. Brain metabolite profiling revealed characteristic biosignatures discriminating Ndufs4GT/GT from WT mice. Specifically, we observed a reversed TCA cycle flux and rewiring of amino acid metabolism in the prefrontal cortex. Next, exposing mice to chronic variable stress (a model for depression-like behavior), we found that Ndufs4GT/GT mice showed altered stress response and coping strategies with a robust stress-associated reprogramming of amino acid metabolism. Our data suggest that impaired mitochondrial CI function is a candidate driver for altered stress reactivity and stress-induced brain metabolic reprogramming. These changes result in unique phenomic and metabolomic signatures distinguishing groups based on their mitochondrial genotype

RAGE does not contribute to renal injury and damage upon ischemia/reperfusion-induced injury.

Item does not contain fulltextThe receptor for advanced glycation end products (RAGE) mediates a variety of inflammatory responses in renal diseases, but its role in renal ischemia/reperfusion (I/R) injury is unknown. We showed that during renal I/R, RAGE ligands HMGB1 and S100B are expressed. However, RAGE deficiency does not affect renal injury and function upon I/R-induced injury

Devonian vertebrates from the Canning and Carnarvon Basins with an overview of Paleozoic vertebrates of Western Australia

A diverse vertebrate fauna, comprising both micro- and macrovertebrate remains, is known from the Paleozoic of Western Australia. However, it is the Late Devonian fauna of the Gogo Formation that shows exceptional preservation and which is the best known. Advances in tomographic techniques, both micro-CT and synchrotron, have revealed new histological data providing information on bone growth, muscle attachments and the evolution of teeth. The fishes from the Gogo Formation have also revealed new information on the evolution of reproductive structures and live birth in early vertebrates. Recent work on the Frasnian reefs that crop out along the Lennard Shelf and mineral drillcore through Paleozoic sedimentary rocks have yielded scales of agnathan thelodonts, and the bones, teeth and scales of sharks, acanthodians and osteichthyans, all of which have increased our knowledge of Ordovician–Late Devonian microfaunas in the Canning Basin, contributing to our understanding of biostratigraphy and correlation within Australia and globally. Less work has been undertaken in the Carnarvon Basin, although like the Canning Basin this has concentrated on Late Devonian strata. More recently, work has commenced on describing Early Carboniferous faunas from the Canning, Carnarvon and Bonaparte Basins. All this work is providing information on faunal patterns and exchange of vertebrates through the Paleozoic. However, the paleogeographic evidence provided by the vertebrates is sometimes at odds with paleogeographic reconstructions based on paleomagnetic evidence and further investigation is required to resolve these differing interpretations

Late Devonian carbonate magnetostratigraphy from the Oscar and Horse Spring Ranges, Lennard Shelf, Canning Basin, Western Australia

The Late Devonian was a time of major evolutionary change encompassing the fifth largest mass extinction, the Frasnian–Famennian event. In order to establish a chronological framework for global correlation before, during, and following the Frasnian–Famennian mass extinction, we carried out a coupled magnetostratigraphic and biostratigraphic study of two stratigraphic sections in the Upper Devonian carbonate reef complexes of the Lennard Shelf, in the Canning Basin, Western Australia. Magnetostratigraphy from these rocks provides the first high-resolution definition of the Late Devonian magnetic polarity timescale. A 581-m-reference section and an 82-m overlapping section through the marginal slope facies (Napier Formation) of the Oscar Range as well as a 117-m section at Horse Spring (Virgin Hills Formation) were sampled at decimeter to meter scale for magnetostratigraphy. Conodont biostratigraphy was used to correlate both sections, and link magnetostratigraphic polarity zones to a globally established biostratigraphy. A stable, Characteristic Remanent Magnetization (ChRM) with dual polarities (NE, shallowly upward and SW, shallowly downward) is recovered from ∼60%∼60% of all samples, with magnetite inferred to be the chief magnetic carrier from thermal demagnetization characteristics. These directions define a geomagnetic pole at 49.5°S/285.8°E and α95=2.4α95=2.4 (n=501n=501), placing the Canning Basin at 9.9°S during the Late Devonian, consistent with carbonate reef development at this time.A conservative interpretation of the magnetostratigraphy shows the recovery of multiple reversals from both sections, not including possible cryptochrons and short duration magnetozones. Field tests for primary remanence include positive reversal tests and matching magnetozones from an overlapping section in the Oscar Range. A strong correlation was found between magnetic polarity stratigraphies of the Oscar Range and Horse Spring sections, and we correlate 12 magnetostratigraphic packages. The relative stratigraphic thicknesses of the isochronous sediments from these two sections indicate that carbonate accumulation was ∼4.5× faster in the middle slope deposits at Oscar Range than in the more distal, lower slope Horse Spring deposits for the middle Frasnian through Famennian. The magnetic field during the Late Devonian underwent a relatively high reversal frequency with good potential for regional and global correlation, and should prove useful in deciphering a high-resolution chronostratigraphy across the Lennard Shelf to enable higher confidence examination of reef development across a major biotic crisis