Reduction of Energetic Demands through Modification of Body Size and Routine Metabolic Rates in Extremophile Fish

Citation: Passow, C. N., Greenway, R., Arias-Rodriguez, L., Jeyasingh, P. D., & Tobler, M. (2015). Reduction of Energetic Demands through Modification of Body Size and Routine Metabolic Rates in Extremophile Fish. Physiological and Biochemical Zoology, 88(4), 371-383. doi:10.1086/681053Variation in energy availability or maintenance costs in extreme environments can exert selection for efficient energy use, and reductions in organismal energy demand can be achieved in two ways: reducing body mass or metabolic suppression. Whether long-term exposure to extreme environmental conditions drives adaptive shifts in body mass or metabolic rates remains an open question. We studied body size variation and variation in routine metabolic rates in locally adapted populations of extremophile fish (Poecilia mexicana) living in toxic, hydrogen sulfide-rich springs and caves. We quantified size distributions and routine metabolic rates in wild-caught individuals from four habitat types. Compared with ancestral populations in nonsulfidic surface habitats, extremophile populations were characterized by significant reductions in body size. Despite elevated metabolic rates in cave fish, the body size reduction precipitated in significantly reduced energy demands in all extremophile populations. Laboratory experiments on common garden-raised fish indicated that elevated routine metabolic rates in cave fish likely have a genetic basis. The results of this study indicate that adaptation to extreme environments directly impacts energy metabolism, with fish living in cave and sulfide spring environments expending less energy overall during routine metabolism

The optical system of the Tenerife Microwave Spectrometer: a window for observing the 10-20 GHz sky spectra

The TMS optical system is based on a decentered dual-reflector system in a Gregorian configuration to observe with an angular resolution of less than $2^\circ$. The primary goal of the present study is to evaluate the final design and verify that it satisfies the design requirements. We aim for low cross-polarization (-30 dB), low sidelobe (-25 dB) levels, and a stable beam in terms of shape (low ellipticity) and size over a full octave bandwidth (10-20 GHz). We performed both ray-tracing and full-wave simulations using the CST Studio software in order to investigate the system behaviour. We gave special attention to the beam frequency variation and polarization leakage. We have characterized the effects on the radiation pattern produced by the cryostat window. We present the final design of the TMS optical system, as well as a complete study of the system's performance in terms of cross-polarization, sidelobes, ellipticity and beamwidth. We discuss the effects of sidelobes and study the need for a baffle.Comment: 30 pages, 15 figures. Accepted for publication in JINS

A Portable Microwave Scanner for Brain Stroke Monitoring: Design, Implementation and Experimental Validation

This paper presents the design, the realization, and the experimental assessment of a novel portable microwave scanner prototype for brain stroke monitoring. The device employs a 22-antenna-array, placed conformal to the upper head part, composed of compact, flexible, and custom-made antennas working at around 1 GHz. The validation includes the monitoring of an experimentally emulated evolving hemorrhagic stroke. The progression of the medical condition is emulated via a non-static phantom (custom-shape balloon), derived from medical images, and a single-cavity 3-D anthropomorphic head phantom. The phantoms are filled with liquids mimicking the dielectric properties of the hemorrhage and the average brain tissues, respectively. The imaging-based follow-up is approached using a differential scheme that receives the scattering matrices, taken at two different instants, and exploits the distorted Born approximation to form the image in real-time. The kernel of the imaging algorithm is computed through accurate numerical models. The results verify the capabilities of the system to assess the continuous evolution of the stroke

Hydrodynamic identification of NAUTILUS FOWT platform from small scale tests

A small-scale tank test campaign of the NAUTILUS offshore wind floating semisub-mersible platform was held at the Ifremer Deep Water Basin within the framework of the MaRINET 2 project. The support structure consists in four stabilized columns on a square pontoon supporting a generic 8-MW wind turbine. The tests were carried out at 1:36 Froude scale in parked conditions, and the mooring system was modelled as a set of aerial mooring springs providing a nonlinear stiffness. The hydrodynamic characterization of the floater from experimental data was tackled by using traditional techniques in naval architecture), as well as approaches derived from operational modal analysis in the frequency domain, such as the Sub Space Identification—Covariance (SSI-COV) method. The validity of this approach and its potential application to the identification of such kind of structures is discussed against the results of a more traditional technique based on the fitting of decay tests

Antioxidant vitamins increase the collagen content and reduce MMP-1 in a porcine model of atherosclerosis: implications for plaque stabilization

Degradation of extracellular matrix, particularly interstitial collagen, promotes plaque instability and contributes to restenosis after vascular injury. We have explored the effects of vitamins C and E on the collagen content and metalloproteinase-1 (MMP-1) expression after angioplasty in hypercholesterolemic pigs. Iliac angioplasty was performed on 18 minipigs divided into three diet groups: a normal-cholesterol (NC), a high-cholesterol (HC) and a high-cholesterol plus vitamins C+E (HCV). Four weeks later, after sacrifice, the vascular collagen content and MMP-1 protein expression, along with the plasma caseinolytic activity and lipid peroxidation, were measured. MMP-1 was also determined in arterial rings stimulated with native low-density lipoproteins (LDL) isolated from experimental groups. Cholesterol-rich diet augmented plasma lipid peroxidation (P<0.05), reduced the collagen content and increased vascular MMP-1 expression after injury (P<0.05). Enhanced caseinolytic activity (identified as MMP-1) was also observed in HC plasma samples and in supernatants from arterial rings incubated with HC-LDL. Vitamins C and E markedly increased neointimal collagen content (P<0.01), reduced the hypercholesterolemia-induced changes in vascular MMP-1 (P<0.05) and diminished plasma and ex vivo caseinolytic activity. Vitamins C and E may help stabilize atherosclerotic plaque after angioplasty and favor vascular remodeling by increasing collagen content and reducing vascular MMP-1 expression in porcine hypercholesterolemia

Reduced salivary oxytocin after an empathic induction task in Intimate Partner Violence perpetrators: Importance of socio-affective functions and its impact on prosocial behavior

Intimate Partner Violence (IPV) has been linked to difficulties in socio-affective functions. Nevertheless, the underlying psychobiological mechanisms that might be responsible for them remain unclear. Oxytocin (OXT) stands out as an important hormone that may favor the salience of social information, due to its relevance in empathy and prosocial behavior. Thus, the study of salivary OXT (sOXT) may provide further information about potential impairments in social cognition in IPV perpetrators. This study analyzed the effects of an empathic induction task, performed through negative emotion-eliciting videos, on endogenous sOXT levels, mood state, and emotional perception in 30 IPV perpetrators compared to 32 controls. Additionally, we explored their performance on prosocial behavior after the empathic induction task, using Hare''s donation procedure. Lower sOXT levels were found in IPV perpetrators after the task compared to controls, along with a general decreasing tendency in their sOXT levels. Additionally, IPV perpetrators exhibited no change in their mood state and perceived others'' emotions as more positive and less intense. Moreover, the mood state response and alexithymia traits, respectively, positively and negatively predicted the sOXT levels after the empathic induction task in the entire sample. Finally, we did not observe a lower appearance of prosocial behaviors in IPV perpetrators; however, higher sOXT levels after the empathic induction task were found in subjects who donated when considering the whole sample. In sum, IPV perpetrators exhibited differences in their sOXT levels when empa-thizing, compared to controls, with alexithymia and the emotional response potentially explaining the sOXT levels after the task. Furthermore, prosocial behavior was more related to these sOXT levels than to IPV. As our knowledge about the emotional processing of IPV perpetrators increases, we will be better able to develop and include coadjutant treatments in current psychotherapeutic programs, in order to focus on their emotional needs, which, in turn, would reduce the future risk of recidivism

Wax worm saliva and the enzymes therein are the key to polyethylene degradation by Galleria mellonella

Plastic degradation by biological systems with re-utilization of the by-products could be a future solution to the global threat of plastic waste accumulation. Here, we report that the saliva of Galleria mellonella larvae (wax worms) is capable of oxidizing and depolymerizing polyethylene (PE), one of the most produced and sturdy polyolefin-derived plastics. This effect is achieved after a few hours’ exposure at room temperature under physiological conditions (neutral pH). The wax worm saliva can overcome the bottleneck step in PE biodegradation, namely the initial oxidation step. Within the saliva, we identify two enzymes, belonging to the phenol oxidase family, that can reproduce the same effect. To the best of our knowledge, these enzymes are the first animal enzymes with this capability, opening the way to potential solutions for plastic waste management through bio-recycling/up-cycling