Investigation of the cytocidal potential of Rhinella jimi skin methanol extracts

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Context: Amphibian skins have wide variety of biologically active compounds associated with the natural defenses of these animals. Objectives: To study the in vitro anticancer activity of methanol extracts of the skin of Rhinella jimi Stevaux (Anura: Bufonidae). Material and methods: The extract was obtained by cold methanol extraction for 96 h using dried skins (295 mg). The methanol skin extract was dried under reduced pressure, giving a 5.5% yield. In order to test for growth-inhibitory activity, in vitro tests were performed with the following cancer cell lines using concentrations ranging between 0.25-250 mu g/mL of the extract by 48 h: K562 (leukemia), MCF-7 (breast), NCI-ADR (breast with MDR phenotype), UACC-62 (melanoma), NCI460 (lung), PCO3 (prostate), HT-29 (colon), OVCAR (ovary), and 786-0 (kidney). Results: The methanol extract of R. jimi produced a growth inhibition in a dose-dependent manner against the most of the assayed cell lines. In addition to the growth inhibition, the extract induced the cell death in the ovary and colon lines (EC50 0.125 and 0.2 mu g/mL, respectively), demonstrating 100% of inhibition with 2.5 mu g/mL. However, prostate and leukemia cell lines demonstrated less sensitivity, with EC50 of 24 and 235 mu g/mL, respectively. This is the first report about the anticancer activity by natural products from the skin of R. jimi. Conclusions: The methanol extracts of R. jimi significantly affected the growth of several cell lines, demonstrating that these compounds are a potential source of substances that could be utilized in cancer treatments.50810261030FUNCAP (Fundacao Cearense de Apoio ao Desenvolvimento Cientifico and Tecnologico) [0006-00/2006, 9913/06]Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Brazilian Institute for the Environment and Natural Resources (IBAMA) [066/06-COFAN IBAMA/RAN/02007.001009/04-37]Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)FUNCAP (Fundacao Cearense de Apoio ao Desenvolvimento Cientifico and Tecnologico) [0006-00/2006, 9913/06]Brazilian Institute for the Environment and Natural Resources (IBAMA) [066/06-COFAN IBAMA/RAN/02007.001009/04-37

Climate change and coastal hydrographic response along the Atlantic Iberian margin (Tagus Prodelta and Muros Ria) during the last two millennia

13 pages, 6 figures, 2 tables.The Tagus Prodelta (W Portugal) and the Muros Ría (NW Spain) are areas of high deposition rates registering high-resolution palaeoclimatic records for western Iberia. We compare the climatic conditions of the two areas over the last two millennia based on proxies of temperature (sea surface temperatures and oxygen isotopes), continental input (grain size, iron and magnetic susceptibility) and productivity (inorganic and organic carbon, carbon isotopes, benthic foraminifera and diatoms). Biogeochemical changes in the Tagus Prodelta reflect widely recognized North Atlantic climatic periods encompassing the Roman Period (AD 0-350), the Dark Ages (AD 400-700), the ‘Mediaeval Warm Period’ (MWP; AD 800-1200) and the ‘Little Ice Age’ (LIA; AD 1300-1750). The atmospheric North Atlantic Oscillation (NAO) drives the Tagus Prodelta multidecadal, long-term variability in precipitation-river input during cold periods (negative NAO) and marine upwelling during warmer periods (positive NAO), a scheme that is reversed in the Galician region. The Muros Ría shows only local hydrodynamics until AD 1150, including a ‘suboxic’ event in the inner Ría around AD 500-700. Since AD 1150 Atlantic warm upwelled waters have ventilated the outer Ría but only reach the inner Ría at AD 1750. The twentieth-century records are also interpreted as a reflex of the inverse NAO mode in both areas, resulting in amplification of the LIA biogeochemical water conditions. Centennial-scale solar activity appears to be another important forcing mechanism (or the only one, if solar activity drives the NAO and ‘Bond-cycles’) behind changes in the hydrography of the Tagus Prodelta, and primary production, bottom ventilation and organic carbon degradation in the Muros Ría.We are grateful to participants and crews of Discovery 249, Poseidon PALEO 1, and B/O Mytilus cruises, and for laboratory technical assistance in grain size, LECO, isotopes, foraminifera and diatoms at the INETI-Laboratory of Marine Geology. Thanks to C. Kissel for the magnetic susceptibility measurements in Gif-sur-Yvette, to M. Siegl for isotope measurements and the ARI-Program at the University of Bremen, to E. Salgueiro and 5. Vaqueiro for XRF measurements at the Bremen Core Repository, to J. Heinemeier for helpful discussions during the construction of age models, to A. Ferreira for critical data discussion and A. Voelker for comments on the manuscript. The comments of an anonymous reviewer helped to improve the final version of this paper. Financial support was provided by EU project HOLSMEER (EVK2-CT-2000-00060). T. Rodrigues was supported by the Fundação para a Ciéncia e Tecnologia, INGMAR project.Peer reviewe