Caracterización del marco institucional del ciclo presupuestario de la Universidad Nacional de Córdoba en términos del modelo de gestión para resultados y recomendaciones para su implementación

Tesis[Magister]--Universidad Nacional de Córdoba, 2021. Título con el que se recibe: Magister en Administración Pública.En esta tesis se caracteriza el marco institucional del ciclo presupuestario de la Universidad Nacional del Córdoba en cuanto a instituciones formales, informales y actores utilizando el ciclo de la gestión para resultados y sus instrumentos. La motivación que impulsa este trabajo se encuentra en la necesidad de entender cómo funciona el ciclo presupuestario de la UNC, cómo se asignan los recursos y si esa asignación responde a una instancia previa de planificación. Para responder estos interrogantes se realizó un relevamiento documental, entrevistas, y un proceso de observación participante. Todo ello sirvió para construir una matriz que permitió caracterizar el marco institucional y los instrumentos de gestión para resultados. Posteriormente, sobre esa caracterización se elaboró un estado de situación respecto de la gestión para resultados, así como unas recomendaciones que abordan, de manera resumida, qué puede hacerse.Fil: Rodríguez De Marco, Diego Alejandro. Universidad Nacional de Córdoba. Facultad de Ciencias Sociales. Instituto de Investigación y Formación en Administración Pública; Argentina

Influence of Tillage and Liming on N2O emission from a rainfed crop

Nitrous oxide (N2O) is the main greenhouse gas (GHG) produced by agricultural soils due to microbial processes. The application of N fertilizers is associated with an increase of N2O losses. However, it is possible to mitigate these emissions by the introduction of adequate management practices (Snyder et al., 2009). Soil conservation practices (i.e.no tillage, NT) have recently become widespread because they promote several positive effects (increases in soil organic carbonand soil fertility, reduction of soil erosion, etc). In terms of GHG emissions, there is no consensus in the literature on the effects of tillage on N2O. Several studies found that NT can produce greater (Baggs et al., 2003), lower (Malhi et al., 2006) or similar (Grandey et al., 2006) N2O emissions compared to traditional tillage (TT). This large uncertainty is associated with the duration of tillage practices and climatic variability. Liming is widely use to solve problems of soil acidity (Al toxicity, yield penalties, etc). Several studies show a decrease in N2O emissions with liming (Barton et al., 2013) whereas no significant effects or increases were observed in others (Galbally et al., 2010). The aim of this work was to evaluate the effects of tillage (NT vs TT) and liming application or not of Ca-amendment) on N2O emissions from an acid soil during a rainfed crop

Integrin Linked Kinase (ILK) Downregulation as an Early Event During the Development of Metabolic Alterations in a Short-Term High Fat Diet Mice Model.

Background/Aims: Diabetes type 2, metabolic syndrome or non-alcoholic fatty liver disease are insulin resistance-related metabolic disorders, which lack a better prognosis before their full establishment. We studied the importance of the intracellular scaffold protein integrin linked kinaes (ILK) as a key modulator in the initial pathogenesis and the early progression of those insulin resistance- related disorders. Methods: Adult mice with a global transgenic downregulation of ILK expression (cKD-ILK) and littermates without that depletion (CT) were fed with either standard (STD) or high fat (HFD) diets during 2 and 6 weeks. Weights, blood glucose and other systemic biochemical parameters were determined in animals under fasting conditions and after glucose or pyruvate intraperitoneal injections to test their tolerance. In RNA or proteins extracted from insulin-sensitive tissues, we determined by reverse transcription?quantitative PCR and western blot the expression of ILK, metabolites transporters and other metabolism and inflammatory markers. Glucose uptake capacity was studied in freshly isolated tissues. Results: HFD feeding was able to early and progressively increase glycaemia, insulinemia, circulating glycerol, body weight gain, liver-mediated gluconeogenesis along this time lapse, but cKD-ILK have all these systemic misbalances exacerbated compared to CT in the same HFD time lapse. Interestingly, the tisular expression of ILK in HFD-fed CT was dramatically downregulated in white adipose tissue (WAT), skeletal muscle and liver at the same extent of the original ILK downregulation of cKD-ILK. We previously published that basal STD-fed cKD-ILK compared to basal STD-CT have different expression of glucose transporters GLUT4 in WAT and skeletal muscle. In the same STD-fed cKD-ILK, we observed here the increased expressions of hepatic GLUT2 and WAT pro-inflammatory cytokines TNF-? and MCP-1. The administration of HFD exacerbated the expression changes in cKD-ILK of these and other markers related to the imbalanced metabolism observed, such as WAT lipolysis (HSL), hepatic gluconeogenesis (PCK-1) and glycerol transport (AQP9). Conclusion: ILK expression may be taken as a predictive determinant of metabolic disorders establishment, because its downregulation seems to correlate with the early imbalance of glucose and glycerol transport and the subsequent loss of systemic homeostasis of these metabolites.Instituto de Salud Carlos III-ISCIIIComunidad de MadridFondo Europeo de Desarrollo Regional-FEDERInstituto Ramon y Cajal de Investigación Sanitária-IRYCISFundación Renal Iñigo Álvarez de Toledo-FRIA

The Integrin Beta1 Modulator Tirofiban Prevents Adipogenesis and Obesity by the Overexpression of Integrin-Linked Kinase: a Pre-Clinical Approach In Vitro and In Vivo

de Frutos, S., Griera, M., Hatem-Vaquero, M. et al. The integrin beta1 modulator Tirofiban prevents adipogenesis and obesity by the overexpression of integrin-linked kinase: a pre-clinical approach in vitro and in vivo. Cell Biosci 12, 10 (2022)Background: Obesity is caused by the enlargement of the white adipose tissue (WAT) depots, characterized by the hypertrophic enlargement of malfunctioning adipocytes within WAT which increases the storage of triglycerides (TG) in the lipid droplets (LD). Adipogenesis pathways as well as the expression and activity of some extracellular matrix receptors integrins are upregulated. Integrin?1 (INTB1) is the main isoform involved in WAT remodeling during obesity and insulin resistance-related diseases. We recently described Integrin Linked Kinase (ILK), a scafold protein recruited by INTB1, as an important mediator of WAT remodeling and insulin resistance. As the few approved drugs to fght obesity have brought long-term cardiovascular side efects and given that the consideration of INTB1 and/or ILK modulation as anti-obesogenic strategies remains unexplored, we aimed to evaluate the anti-obesogenic capacity of the clinically approved anticoagulant Tirofban (TF), stated in preclinical studies as a cardiovascular protector. Methods: Fully diferentiated adipocytes originating from C3H10T1/2 were exposed to TF and were co-treated with specifc INTB1 blockers or with siRNA-based knockdown ILK expression. Lipid-specifc dyes were used to determine the TG content in LD. The genetic expression pattern of ILK, pro-infammatory cytokines (MCP1, IL6), adipogenesis (PPAR?, Leptin), thermogenesis (UCP1), proliferation (PCNA), lipid metabolism (FASN, HSL, ATGL), and metabolite trans porters (FABP4, FAT, AQP7) were detected using quantitative PCR. Cytoskeletal actin polymerization was detected by confocal microscopy. Immunoblotting was performed to detect INTB1 phosphorylation at Thr788/9 and ILK activity as phosphorylation levels of protein kinase B (AKT) in Ser473 and glycogen synthase kinase 3? (GSK3?) at Ser9. TF was intraperitoneally administered once per day to wildtype and ILK knockdown mice (cKDILK) challenged with a high-fat diet (HFD) or control diet (STD) for 2 weeks. Body and WAT weight gains were compared. The expression of ILK and other markers was determined in the visceral epididymal (epi) and inguinal subcutaneous (sc) WAT. Results: TF reduced TG content and the expression of adipogenesis markers and transporters in adipocytes, while UCP-1 expression was increased and the expression of lipases, cytokines or PCNA was not afected. Mechanistically, TF rapidly increased and faded the intracellular phosphorylation of INTB1 but not AKT or GSK3?. F-actin levels were rapidly decreased, and INTB1 blockade avoided the TF efect. After 24 h, ILK expression and phosphorylation rates of AKT and GSK3? were upregulated, while ILK silencing increased TG content. INTB1 blockade and ILK silencing avoided TF efects on the TG content and the transcriptional expression of PPAR? and UCP1. In HFD-challenged mice, the systemic administration of TF for several days reduced the weight gain on WAT depots. TF reduced adipogenesis and pro-infammatory biomarkers and increased lipolysis markers HSL and FAT in epiWAT from HFD, while increased UCP1 in scWAT. In both WATs, TF upregulated ILK expression and activity, while no changes were observed in other tissues. In HFD-fed cKDILK, the blunted ILK in epiWAT worsened weight gain and avoided the anti-obesogenic efect of in vivo TF administration. Conclusions: ILK downregulation in WAT can be considered a biomarker of obesity establishment. Via an INTB1-ILK axis, TF restores malfunctioning hypertrophied WAT by changing the expression of adipocyte-related genes, increas ing ILK expression and activity, and reducing TG storage. TF prevents obesity, a property to be added to its anticoagu lant and cardiovascular protective advantages.Instituto de Salud Carlos IIIComunidad de MadridFondo Europeo de Desarrollo Regional-FEDE

Relevamiento de necesidades de capacitación. Propuesta de un nuevo plan de formación en consulta con niveles directivos de la SPGI y dependencias

Este proyecto tiene por propósito lograr que el personal técnico, administrativo y de servicios de las dependencias eleve su nivel de formación para contribuir al funcionamiento eficiente y coordinado de la SPGI brindando el soporte para la consecución de sus objetivos y su visión.Fil: Robledo, Luciana. Universidad Nacional de Córdoba. Secretaría de Planificación y Gestión Institucional. Área de Recursos Humanos, Argentina.Fil: Perdiguero, Patricia. Universidad Nacional de Córdoba. Secretaría de Planificación y Gestión Institucional. Dirección de General de Contabilidad y Finanzas, Argentina.Fil: Castellano, Paola. Universidad Nacional de Córdoba. Secretaría de Planificación y Gestión Institucional. Dirección de General de Contabilidad y Finanzas, Argentina.Fil: Pucheta, Marina. Universidad Nacional de Córdoba. Secretaría de Planificación y Gestión Institucional. Dirección de General de Contabilidad y Finanzas, Argentina.Fil: Rodríguez de Marco, Diego. Universidad Nacional de Córdoba. Secretaría de Planificación y Gestión Institucional. Dirección de General de Contabilidad y Finanzas, Argentina

Blocking ephrinB2 with highly specific antibodies inhibits angiogenesis, lymphangiogenesis, and tumor growth

Membrane-anchored ephrinB2 and its receptor EphB4 are involved in the formation of blood and lymphatic vessels in normal and pathologic conditions. Eph/ephrin activation requires cell-cell interactions and leads to bidirectional signaling pathways in both ligand- and receptor-expressing cells. To investigate the functional consequences of blocking ephrinB2 activity, 2 highly specific human single-chain Fv (scFv) Ab fragments against ephrinB2 were generated and characterized. Both Ab fragments suppressed endothelial cell migration and tube formation in vitro in response to VEGF and provoked abnormal cell motility and actin cytoskeleton alterations in isolated endothelial cells. As only one of them (B11) competed for binding of ephrinB2 to EphB4, these data suggest an EphB-receptor–independent blocking mechanism. Anti-ephrinB2 therapy reduced VEGF-induced neovascularization in a mouse Matrigel plug assay. Moreover, systemic administration of ephrinB2-blocking Abs caused a drastic reduction in the number of blood and lymphatic vessels in xenografted mice and a concomitant reduction in tumor growth. Our results show for the first time that specific Ab-based ephrinB2 targeting may represent an effective therapeutic strategy to be used as an alternative or in combination with existing antiangiogenic drugs for treating patients with cancer and other angiogenesis-related diseases.This work was supported by grants from the Regional Government of Madrid, Spain (Angiobodies Programmes, S-BIO-0236-2006 and S2010/BMD-2312).Peer reviewe

Design culture:from product to process. Building a network to develop design processes in Latin countries

The essay presents the results of a 2 years survey within 7 European and Latin American countries (Italy, Portugal, Spain, Argentina, Brazil, Chile, Mexico), focusing on the scientific and professional initiatives accomplished by universities and research centres in the field of designdriven innovation. The survey shows a growing interest towards the methodological aspects of design by the observed actors and urges the formation of a "Latin network" aimed at experimenting proper design processes for Latin countries' socio-cultural and productive systems. The basic idea is that design culture, considered as a process culture, has relevant advantages in the actual relationship between production and consumption: − Offering design a mediator's role between the crucial knowledge for sustainable development: economy, art/creativity, technology and humanities; − Producing a "phantasmagoric" capacity (the possibility to visualize and share what is immaterial and upcoming); − Mediating between the productive and consumption systems' interest

Comparación de la potencia aeróbica máxima e indicadores de saltabilidad según sexo en estudiantes de octavo básico y segundo medio del Colegio San Francisco Javier de Huechuraba

Tesis (Profesor de Educación Física, Licenciado en Educación)Actualmente, Chile presenta elevados índices de obesidad y enfermedades asociadas al sedentarismo, siendo la actividad física una de las principales maneras de prevenir y contrarrestar sus consecuencias negativas. Esta problemática también se observa al interior de los establecimientos educacionales, donde una correcta planificación de las actividades físicas permite maximizar sus efectos positivos sobre la salud física y mental de los estudiantes. Para ello, es relevante realizar un diagnóstico y recabar información respecto a la situación actual en términos de aptitud física en los estudiantes de un determinado colegio, y cómo el sexo influye sobre dicho parámetro. En la presente tesis se realizó un estudio comparativo para determinar las diferencias que existen entre distintos sexos en cursos de octavo y segundo medio, al momento de realizar un esfuerzo físico de tipo aeróbico y anaeróbico. Para ello, se utilizó el test Course Navette y la batería de saltos creada por el profesor Carmelo Bosco, respectivamente, comparando las diferencias observadas entre hombres y mujeres de los mencionados cursos en el colegio San Francisco Javier de Huechuraba. En base a la aplicación de los diferentes tests, se observó que el promedio de varones fue superior al de las damas, es decir, el sexo masculino obtuvo mejores resultados en las distintas pruebas de resistencia aeróbica y de saltos, lo que podría estar relacionado a las diferencias fisiológicas existentes entre ambos sexos. Los resultados anteriores evidencian la necesidad de generar nuevas pautas y proyectos donde se plantean ejercicios de forma más personalizada y acorde al nivel de maduración del sexo, evaluando de distinta manera a hombres y mujeres. Las conclusiones generadas a partir de esta tesis permitieron además ampliar un camino de investigación, recalcando la necesidad y utilidad de abarcar distintos temas relacionados a cómo se están desarrollando los estudiantes y qué caminos de acción y evaluación se debe tomar como profesores para entregar las mejores herramientas, formando jóvenes activamente motrices y capaces de realizar tanto actividad física en la vida cotidiana como desempeñarse en algún deporte de elite.Currently, Chile country has a very high prevalence of obesity and sedentarism-associated diseases, being the physical activity one of the principal of preventing and counteracting its negative consequences. This problem is also observed in educational establishment, where a correct planing of the physical activity programs allows to a maximization of its beneficial effects on the mental and physical health of students. For this, it is very important to diagnose and gather information the current situation in terms of physical condition of students from a defined school, and how gender influences such parameter. The thesis of this work was comparative study to determine the existing gender differences in eight and tenth graders of each gender inresponse to aerobic and anaerobic excercises. For this purpose, we used the Curse Navette test and the jump battery test developed by the professor Carmelo Bosco, respectively, comparing the differences observed between men and women from the grades mentionaed above in the school San Francisco of Huechuraba. Based on the application of different tests, observed that the average of physical condition was higher in men compared to women in both aerobic and jump resistence tests, and this could be related with the physiological differences that exists between both genders. These results ilustrate the importance of generating new excercise programs, tending to a personalized routine in agreement with sex maturation, and evaluating men and women distinctly. The conclusions generated from this thesis allowed us the exploration of to the problem from a wider point of view, evidenciating the importance and utility of discussing how students are learning which teaching strategies teaching and evaluations should be follow by teachers, to provide the best learning tools to form active and healthy young people, able to practice both routinary or domestic exercise programs and a high-performance sport

Siwa: A custom RISC-V based system on chip (SOC) for low power medical applications

This work introduces the development of Siwa, a RISC-V RV32I 32-bit based core, intended as a flexible control platform for highly integrated implantable biomedical applications, and implemented on a commercial 0.18 m high voltage (HV) CMOS technology. Simulations show that Siwa can outperform commercial micro-controllers commonly used in the medical industry as control units for implantable devices, with energy requirements below the 50 pJ per clock cycle.Agencia Nacional de Investigación e Innovació

Siwa: a RISC-V RV32I based micro-controller for implantable medical applications

The design of Siwa1, a compact low power custom system on chip (SoC), targeted for implantable/wearable applications, is reported in this paper. Siwa is based on a RISC-V RV32I architecture. It has a centrally controlled non-pipelined structure, and it includes a control interface for an integrated sensing and stimulation device for biological tissues as well as standard communication interfaces. Siwa was developed from scratch using System Verilog, and implemented in a 180nm CMOS technology; Siwa includes a latch based register file c apable to read and write in one clock cycle with an area 30% smaller and a power consumption 25% lower with respect to an equivalent flip flop implementation; also, it has an estimated average power consumption of 70μW (48pJ/cycle) which is comparable to other micro-controllers commonly used in IMD applications.Agencia Nacional de Investigación e Innovació