Homocysteine treatment alters redox capacity of both endothelial and tumor cells

Homocysteine is a non-proteinogenic amino acid playing key roles in two interconnected metabolic pathways, namely, the activated methyl cycle and the linear trans-sulfuration pathway that allows the conversion of methionine to cysteine. A dysregulation of intracellular homocysteine metabolism could yield an increased export of this amino acid, leading to hyperhomocysteinemia, which has been associated with an increased risk of cardiovascular diseases. In spite of decades of experimental effort, there is no definitive consensus on what could be the molecular mechanisms whereby hyperhomocysteinemia could contribute to cardiovascular disease. The redox active nature of homocysteine has favored the idea of an induction of oxidative stress as the underlying mechanism of homocysteine toxicity. In contrast, homocysteine can also behave as an anti-oxidant. The present work is aimed to further analyze the capacity of homocysteine to modulate the redox capacity of both endothelial and tumor cells. [Our experimental work is supported by grants BIO2014-56092-R (MINECO and FEDER) and P12-CTS-1507 (Andalusian Government and FEDER) and funds from group BIO-267 (Andalusian Government). The "CIBER de Enfermedades Raras" is an initiative from the ISCIII (Spain)].Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

THE RELATION BETWEEN DEPRESSION AND PERCEIVED SOCIAL SUPPORT IN NURSING STUDENTS IN THE CONTEXT OF SUICIDAL BEHAVIORS

Objective: to determine the relationship between depression and perceived social support in nursing students in the context of suicidal behaviors.Method: secondary analysis of data from a previous study. Sample from the census of nursing students in Costa Rica. Data collection performed by LimeSurveyduring 2020 by means of self-administered questionnaire composed of sociodemographic data section, Beck-II depression inventory and multidimensional scale of perceived social support. Descriptive analysis and Pearson correlation were used.Results: The majority were female (79.5%), with a mean age of 22.15 years; 85.8% reported mild to minimal depression; 92.2% reported high perceived social support. A significant inverse correlation was identified between level of depression and perceived social support (r=-0.44, p<0.01).Conclusion: The understanding about mental health conditions by nursing science is broadened to improve care practices in a specific group

Study of humoral and cellular immunity in vaccinated with mRNA-1273

This work was supported by "Investigacion y Desarrollo (I + D) del Sistema Andaluz de Salud (SAS)" and Instituto de Salud Carlos III (Proyecto FIS PI21/01708).The new vaccines against SARS-CoV-2 have raised a lot of expectations about their ability to induce immunity and the duration of this. This is the case of mRNA vaccines such as Moderna’s mRNA-1273. Therefore, it is necessary to study the humoral and cellular immunity generated by these vaccines. Our objectives are determining what is the normal response of antibody production, and what is the level of protective antibodies and monitoring patients in case of subsequent infection with COVID-19. We present the first results of a longitudinal study of the humoral response in 601 health workers vaccinated with Moderna. The results show a humoral immunity at 90 days after the second dose of 100%, with a strong decrease between the levels of circulating anti-S IgG antibodies between days 30 and 90 postvaccination. Observing a steeper decline in those who had higher titles at the beginning. In addition, we present a cellular response of 86% at three months after the second dose, which is related to low humoral response.Investigacion y Desarrollo (I + D) del Sistema Andaluz de Salud (SAS)Instituto de Salud Carlos III European Commission FIS PI21/0170

Neuromonitorización de la profundidad anestésica y nocicepción

A lo largo de las últimas décadas, la anestesia ha experimentado considerables cambios. En un intento por mejorar la práctica anestésica se han desarrollado diversos monitores con el objetivo de controlar con mayor precisión y objetividad el nivel de profundidad anestésica y de la nocicepción. Entre los monitores más extendidos se encuentran el Índice Biespectral, Módulo de Entropía, Nociception Level Index y Surgical Pleth Index. Los monitores actuales se basan en los datos derivados de la interacción entre los anestésicos y el sistema nervioso central y autónomo. Además, su uso podría extenderse fuera del quirófano, en las Unidades de Cuidados Intensivos. Continuar la investigación en este campo podría suponer un gran avance en el desarrollo de la "anestesia a medida", beneficiándose cada paciente de las ventajas de la anestesia pero disminuyendo al máximo sus efectos adversos.<br /

RELACIÓN DEPRESIÓN Y APOYO SOCIAL PERCIBIDO EN ESTUDIANTES DE ENFERMERÍA EN EL CONTEXTO DE CONDUCTAS SUICIDAS

Objetivo: determinar la relación entre depresión y apoyo social percibido en estudiantes de enfermería en el contexto de las conductas suicidas.Método: análisis secundario de datos de un estudio previo. Muestra censal en estudiantes de enfermería de Costa Rica. Recolección de datos realizada por LimeSurvey durante el 2020 con una encuesta autoadministrada compuesta de sección de datos sociodemográficos, inventario de depresión de Beck-II y escala multidimensional de apoyo social percibido. Se utilizó análisis descriptivo y correlación de Pearson.Resultados: la mayoría era del género femenino (79,5%), con un promedio de 22,15 años. El 85,8% manifestó un nivel mínimo-leve de depresión, el 92,2% evidenció un apoyo social percibido alto. Se identificó una correlación inversa significativa entre nivel de depresión y apoyo social percibido (r=-0.44, p<0.01).Conclusión: se amplía la comprensión de condiciones de salud mental desde la ciencia de enfermería que podría mejorar las prácticas de cuidado en un grupo específico.

RELAÇÃO ENTRE A DEPRESSÃO E O APOIO SOCIAL PERCEBIDO NOS ESTUDANTES DE ENFERMAGEM NO CONTEXTO DE COMPORTAMENTOS SUICIDAS

Objetivo: determinar a relação entre a depressão e o apoio social percebido nos estudantes de enfermagem no contexto de comportamentos suicidas.Método: análise secundária de dados de um estudo anterior. Amostra do censo dos estudantes de enfermagem na Costa Rica. Coleta de dados realizada por LimeSurvey during 2020 por meio de questionário autoadministrado composto por seção de dados sociodemográficos, inventário de depressão Beck-II e escala multidimensional de apoio social percebido. Foram utilizadas a análise descritiva e a correlação de Pearson.Resultados: A maioria era do sexo feminino (79,5%), com idade média de 22,15 anos; 85,8% referiram depressão ligeira a mínima; 92,2% referiram elevado apoio social percebido. Foi identificada correlação inversa significativa entre o nível de depressão e a percepção do apoio social (r=-0,44, p<0,01).Conclusão: Amplia-se a compreensão acerca das condições de saúde mental por parte da ciência da enfermagem para que se possa melhorar as práticas de cuidados em um grupo específico

Antiangiogenic potential of an anthraquinone metabolite isolated from a marine fungus.

This is a communication in the format of a posterThe potential of marine organisms to yield bioactive molecules is vast and largely unexplored. Significantly, certain bioactive compounds derived from the marine environment have already received approval as anticancer drugs1. Among the multitude of bioactive compounds found in marine organisms, anthraquinones represent a notable class of molecules, with over 200 structurally related compounds having been isolated from diverse species of marine fungi2. Danthron (1,8-dihydroxy-9,10-anthraquinone) serves as an exemplary member of anthraquinones, possessing anti-tumoral and anti-angiogenic properties that have yet to be fully elucidated. Therefore, the primary objective of this study was to comprehensively investigate and understand these specific properties.Funding: Funding: Projects PID2019-105010RB-I00 (Ministry of Science and Innovation, Government of Spain), UMA18-FEDERJA-220 and P20_00257 (Junta de Andalucía-FEDER) The assistance to the congress has an help from the Plan Propio de Investigación (UMA). Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Antioxidant potential of an anthraquinone metabolite isolated from a marine fungus.

Marine ecosystem is a fruitful source of many pharmacologically active. In particular, a number of marine anthraquinones and their derivatives, have been studied for their potential as anticancer and antimetastatic agents, although little is known about their antiangiogenic activities. In addition, there is some controversy regarding the poor antioxidant effect of these marine secondary metabolites. In this study, we describe for the first time the antiangiogenic activity of an anthraquinone, isolated from a marine fungus, which was evidenced in vitro by the inhibition of the activated endothelial cells survival, proliferation, and differentiation into tubelike structures. Furthermore, we have demonstrated its protective effect against reactive oxygen species (ROS), especially in presence of hydrogen peroxide, a potent prooxidant agent. These results were evidenced by a reduced ROS production and an increased redox capacity of cells after treatment with this anthraquinone, evidenced through the staining with DCFHDA and the sulfhydryl groups determination, respectively. The observation that this compound appears to present a more potent effect in tumor than in endothelial cells, suggests a putative selectivity. All these results highlight the need for further study of this molecule as a potential therapeutic agent for cancer and other angiogenesisdependent diseases.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Finite element modeling of temperature fields on the cutting edge in the dry high-speed turning of aisi 1045 steel

High-speed turning is an advanced and emerging machining technique that, in contrast to the conventional machining, allows the manufacture of the workpiece with high accuracy, efficiency and quality, with lower production costs and with a considerable reduction in the machining times. The cutting tools used for the conventional machining cannot be employed for high-speed machining due to a high temperature induced in machining and a lower tool life. Therefore, it is necessary to study the influence of high cutting speeds on the temperature distribution in different typologies of cutting tools, with the aim of evaluating their behavior. In this paper, a finite element method modeling approach with arbitrary Lagrangian-Eulerian fully coupled thermal-stress analysis is employed. The research presents the results of different cutting tools (two coated carbide tools and uncoated cermet) effects on average surface temperature fields on the cutting edge in the dry high-speed turning of AISI 1045 steel. The numerical experiments were designed based on different cutting tools like input parameters and different temperature field zones like dependent variables in the dry high-speed turning of AISI 1045 steel. The results indicate that the dry high-speed turning of AISI 1045 steel does not influence significantly the temperature field zones when P10, P15 or P25 inserts are used. Therefore, the use of a dry high-speed turning method, which reduces the amount of lubricant and increases productivity, may represent an alternative to turning to the extent here described.Postprint (published version

Long-term exhaustion of the inbreeding load in Drosophila melanogaster

Inbreeding depression, the decline in fitness of inbred individuals, is a ubiquitous phenomenon of great relevance in evolutionary biology and in the fields of animal and plant breeding and conservation. Inbreeding depression is due to the expression of recessive deleterious alleles that are concealed in heterozygous state in noninbred individuals, the so-called inbreeding load. Genetic purging reduces inbreeding depression by removing these alleles when expressed in homozygosis due to inbreeding. It is generally thought that fast inbreeding (such as that generated by full-sib mating lines) removes only highly deleterious recessive alleles, while slow inbreeding can also remove mildly deleterious ones. However, a question remains regarding which proportion of the inbreeding load can be removed by purging under slow inbreeding in moderately large populations. We report results of two long-term slow inbreeding Drosophila experiments (125–234 generations), each using a large population and a number of derived lines with effective sizes about 1000 and 50, respectively. The inbreeding load was virtually exhausted after more than one hundred generations in large populations and between a few tens and over one hundred generations in the lines. This result is not expected from genetic drift alone, and is in agreement with the theoretical purging predictions. Computer simulations suggest that these results are consistent with a model of relatively few deleterious mutations of large homozygous effects and partially recessive gene actionAgencia Estatal de Investigación (AEI) | Ref. PGC2018-095810-B-I00Agencia Estatal de Investigación (AEI) | Ref. PID2020-114426GB-C21Xunta de Galicia | Ref. ED431C 2020-0