Effectiveness of the Treatment of Physiotherapy in the Congenital Muscular Torticollis: A Systematic Review

A single congenital muscular torticollis (CMT) is a postural musculoskeletal deformity and is characterized by the shortening or stiffness of the sternocleidomastoid muscle. The reported incidence of CMT ranges from 0.2% to 2%. The objective is to evaluate the effect of physical therapy programs on CMT. For the search, PubMed, Scopus, Web of Science, PEDro and Cochrane databases were used. Randomized controlled trials published between 2018 and 2023 have been included. This study follows the PRISMA 2020 statement and has been registered in the PROSPERO database. Finally, six studies were included. The cervical range of motion (ROM) in rotation was the most analyzed variable, followed by the ultrasound evaluation; one of the studies included the analysis of children’s motor development with the Alberta scale. All research found benefits associated with soft tissue mobilization, passive stretching techniques and manual therapy of the cervical spine. In conclusion, it is possible to recommend manual therapy and passive stretching techniques for the treatment of CMT, with significant results on the cervical ROM

Effectiveness of Percutaneous Electrolysis in Supraspinatus Tendinopathy: A Single-Blinded Randomized Controlled Trial

Supraspinatus tendinopathy is one of the most common causes of shoulder pain. Many studies support conservative treatments such as exercise, trigger point dry needling or corticosteroid injections. Otherwise, a minimally invasive approach with percutaneous electrolysis (PE) has also been used successfully in shoulder pain, although evidence about its long-term effects is scarce. The aim of this trial was to determine the effects of PE on supraspinatus tendinopathy compared with trigger point dry needling (TDN). Thirty-six patients with supraspinatus tendinopathy were randomly assigned to either a PE group (n=18) or a TDN group (n=18). Both groups also performed eccentric exercises. The main outcome to be measured was the Numerical Pain Rating Scale (NPRS), but the shoulder range of motion (ROM) and trigger point pressure pain threshold (PPT) were also considered. A one-year follow-up was conducted. Significant differences favoring the PE group were found regarding pain at one-year follow-up (p=0.002). The improvement achieved in the PE group was greater in the NPRS (p<0.001), proximal PPT, middle PPT, distal PPT (all p<0.001) and ranges of movement. PE seems to be more effective than TDN in relieving pain and improving ROM and PPT supraspinatus values in patients with supraspinatus tendinopathy, both right after treatment and at one-year follow-up

Effectiveness of Active Therapy-Based Training to Improve the Balance in Patients with Fibromyalgia: A Systematic Review with Meta-Analysis

Balance impairment is a frequent disorder in patients with fibromyalgia (FMS), increasing the risk of falls and decreasing physical function and quality of life. In recent years, the use of active therapy-based training (ATBT) has increased, with the aim of improving balance in women with FMS. Our study aimed to assess the effect of ATBT to improve different balance outcomes in subjects with FMS. A systematic review with meta-analysis was carried out. We searched PubMed Medline, SCOPUS, Web of Science, CINAHL, and PEDro (Physiotherapy Evidence Database) databases up to September 2020. We included randomized controlled trials (RCT) that assessed the balance in patients with FMS after ATBT and compared to other treatments or no intervention. In a random-effects model, the standardized mean difference (SMD) was used to calculate the effect size. Ten studies were included in the review providing data from 546 FMS patients with a mean age of 52.41 +/- 2.90 years old (98% females). Our results showed a medium effect favors ATBT with respect to other therapies for monopedal static balance (SMD = 0.571; 95% CI = 0.305, 0.836; p < 0.001), dynamic balance (SMD = 0.618; 95% CI = 0.348, 0.888; p < 0.001), and functional balance (SMD = 0.409; 95% CI = 0.044, 0.774; p = 0.028). No statistically significant differences were found for balance on unstable support. The present meta-analysis showed moderate-quality evidence of a medium effect of ATBT to improve dynamic and functional balance and low-quality evidence of a medium effect to improve monopedal static balance with respect to other therapies or no intervention

Percutaneous Electrolysis in the Treatment of Lateral Epicondylalgia: A Single-Blind Randomized Controlled Trial

Few studies have considered the effects of percutaneous electrolysis (PE) in the treatment of lateral epicondylalgia (LE). For this reason, the objective of this study was to compare the effects of PE with an evidence-based approach-trigger point dry needling (TDN)-in patients with LE. A randomized controlled trial was conducted in which 32 participants with LE were randomly assigned to two treatment groups, the PE group (n= 16) and the TDN group (n= 16). Both groups received four therapy sessions and an eccentric exercise program to be performed daily. The numerical pain rating scale (NPRS), pressure pain thresholds (PPT), quality of life, and range of motion were measured before treatment, at the end of treatment, and at one- and three-month follow-ups. Significant between-group mean differences were found after treatment for NPRS (p< 0.001) and flexion movement (p= 0.006). At one-month follow-up, significant mean differences between groups were found for NPRS (p< 0.001), PPT (p= 0.021), and flexion (p= 0.036). At three-months follow-up, significant mean differences between groups were found for NPRS (p< 0.001), PPT (p= 0.004), and flexion (p= 0.003). This study provides evidence that PE could be more effective than TDN for short- and medium-term improvement of pain and PPTs in LE when added to an eccentric exercise program

Proteomic analysis of goat milk kefir: profiling the fermentation-time dependent protein digestion and identification of potential peptides with biological activity

Kefir is a fermented dairy product, associated to health benefits because of being a probiotic and due to the presence of molecules with biological activity. In this work, we have profiled the peptide composition of goat milk kefir at three different fermentation times using a peptidomics approach, in order to study changes in peptide concentrations and patterns of protein digestion throughout the fermentation time. We identified 2328 unique peptides corresponding to 22 protein annotations, with a maximum of peptides found after 24 h fermentation. We established different digestion patterns according to the nature of the proteins, and quantified the changes in the peptides appearing in all the fermentation times. We also identified 11 peptides that matched exactly to sequences with biological activity in databases, almost all of them belonging to caseins. This is the most comprehensive proteomic analysis of goat milk kefir to date

Cueva de los Torrejones revisited. New insights on the paleoecology of inland Iberia during the Late Pleistocene

34 p.The interior of the Iberian Peninsula has orographic conditions that make this territory especially vulnerable to Quaternary climate oscillations and which actually could have made it decisive for Paleolithic human populations at critical points. For this reason, the information provided by paleontological sites is important for reconstructing climatic and environmental conditions during the Late Pleistocene and understanding how they influenced the species that inhabited them, including humans. Nevertheless, the archaeo-paleontological record is scarce in central Iberia for the Late Pleistocene. A central Iberian site that is key to addressing this issue is Cueva de los Torrejones, which was discovered and excavated during the nineties. Clues indicating the presence of Neandertal populations near the cave site were announced during prior field excavations, including Neandertal remains, Middle Paleolithic artifacts, and evidence of anthropic exploitation of faunal resources at the site. Here we report the new results from the recent excavations and research, including detailed studies on stratigraphy, micromorphology, macro and microvertebrate paleontology, physical and molecular anthropology, taphonomy and zooarchaeology, and analysis of lithic and pottery remains. Our research has led to the detection of three Prehistoric chronologies recorded at the site. The oldest episode corresponds to between MIS 5 and MIS 4 in which the cave was used by carnivores. The second episode is represented by a faunal association dated to 30.0 ka cal BP and is indicative of cooler and more arid environmental conditions and, therefore, compatible with the worsening climate detected previously for MIS 3 in this area. The last episode corresponds to the Chalcolithic, directly dated to ~5000 cal BP in which humans used the cavity for funerary purposes. The DNA analysis of the human remain was assigned to mtDNA haplogroup K, which was originated in the Near East and reached western Europe through the Neolithic expansion. Human occupation during the Paleolithic has been ruled out, including Paleolithic human remains and any kind of anthropic intervention on the Hermann’s tortoise and leopard as was previously proposed at the site.European Research CouncilJunta de Comunidades de Castilla la ManchaMinisterio de Ciencia e InnovaciónCentro Nacional de Investigación sobre la Evolución Humana (CENIEH

Holistic view of biological nitrogen fixation and phosphorus mobilization in Azotobacter chroococcum NCIMB 8003

Nitrogen (N) and phosphorus (P) deficiencies are two of the most agronomic problems that cause significant decrease in crop yield and quality. N and P chemical fertilizers are widely used in current agriculture, causing environmental problems and increasing production costs. Therefore, the development of alternative strategies to reduce the use of chemical fertilizers while maintaining N and P inputs are being investigated. Although dinitrogen is an abundant gas in the atmosphere, it requires biological nitrogen fixation (BNF) to be transformed into ammonium, a nitrogen source assimilable by living organisms. This process is bioenergetically expensive and, therefore, highly regulated. Factors like availability of other essential elements, as phosphorus, strongly influence BNF. However, the molecular mechanisms of these interactions are unclear. In this work, a physiological characterization of BNF and phosphorus mobilization (PM) from an insoluble form (Ca3(PO4)2) in Azotobacter chroococcum NCIMB 8003 was carried out. These processes were analyzed by quantitative proteomics in order to detect their molecular requirements and interactions. BNF led to a metabolic change beyond the proteins strictly necessary to carry out the process, including the metabolism related to other elements, like phosphorus. Also, changes in cell mobility, heme group synthesis and oxidative stress responses were observed. This study also revealed two phosphatases that seem to have the main role in PM, an exopolyphosphatase and a non-specific alkaline phosphatase PhoX. When both BNF and PM processes take place simultaneously, the synthesis of nitrogenous bases and L-methionine were also affected. Thus, although the interdependence is still unknown, possible biotechnological applications of these processes should take into account the indicated factors.Datos de investigación disponibles en: https://www.frontiersin.org/articles/10.3389/fmicb.2023.1129721/full#supplementary-materia

New data on the Quaternary of Navarre: the paleontological collection from Koskobilo (Olazti/Olazagutía)

Libro de resúmenes disponible para su descarga gratuita en el enlace: http://www.aranzadi.eus/catalogo/xv-reunion-nacional-cuaternario-libro-de-resumenesThe archaeopaleontological collection from Koskobilo is composed of hundreds of fossil and thousands of lithic remains, mostly recovered during the middle part of the 20th century. Here we present the complete paleontological study of the collection, which includes 36 mammal taxa, 6 avian taxa and a small fish vertebral collection. Additionally we have performed direct dating of a speleothem crust covering one of the rhinoceros teeth, which provides a minimum of 219 ka for part of the collection, the rest being from the Upper Pleistocene, mixed with some Holocene/recent elements. The paleontological collection from Koskobilo is one of the most important in the Western Pyrenees due to the scarcity of the local Middle Pleistocene fossil record and the rarity of some of the taxa represented, such as Ursus thibetanus, Macaca sylvanus and cf. Megaceroides.La presente investigación ha contado con el apoyo del grupo de investigación IT1044-16 de Eusko Jaurlaritza-Gobierno Vasco, del Grupo PPG17/05 de la Universidad del País Vasco-Euskal Herriko Unibertsitatea y del Ministerio de Ciencia, Innovación y Universidades (proyecto PGC2018-093925-B-C33)

Pseudomonas pseudoalcaligenes CECT5344, a cyanide‑degrading bacterium with by‑product (polyhydroxyalkanoates) formation capacity

Background: Cyanide is one of the most toxic chemicals produced by anthropogenic activities like mining and jewelry industries, which generate wastewater residues with high concentrations of this compound. Pseudomonas pseudoalcaligenes CECT5344 is a model microorganism to be used in detoxification of industrial wastewaters containing not only free cyanide (CN−) but also cyano-derivatives, such as cyanate, nitriles and metal-cyanide complexes. Previous in silico analyses suggested the existence of genes putatively involved in metabolism of short chain length (scl-) and medium chain length (mcl-) polyhydroxyalkanoates (PHAs) located in three different clusters in the genome of this bacterium. PHAs are polyesters considered as an alternative of petroleum-based plastics. Strategies to optimize the bioremediation process in terms of reducing the cost of the production medium are required. Results: In this work, a biological treatment of the jewelry industry cyanide-rich wastewater coupled to PHAs production as by-product has been considered. The functionality of the pha genes from P. pseudoalcaligenes CECT5344 has been demonstrated. Mutant strains defective in each proposed PHA synthases coding genes (Mpha−, deleted in putative mcl-PHA synthases; Spha−, deleted in the putative scl-PHA synthase) were generated. The accumulation and monomer composition of scl- or mcl-PHAs in wild type and mutant strains were confirmed by gas chromatographymass spectrometry (GC–MS). The production of PHAs as by-product while degrading cyanide from the jewelry industry wastewater was analyzed in batch reactor in each strain. The wild type and the mutant strains grew at similar rates when using octanoate as the carbon source and cyanide as the sole nitrogen source. When cyanide was depleted from the medium, both scl-PHAs and mcl-PHAs were detected in the wild-type strain, whereas scl-PHAs or mcl-PHAs were accumulated in Mpha− and Spha−, respectively. The scl-PHAs were identified as homopolymers of 3-hydroxybutyrate and the mcl-PHAs were composed of 3-hydroxyoctanoate and 3-hydroxyhexanoate monomers. Conclusions: These results demonstrated, as proof of concept, that talented strains such as P. pseudoalcaligenes might be applied in bioremediation of industrial residues containing cyanide, while concomitantly generate by-products like polyhydroxyalkanoates. A customized optimization of the target bioremediation process is required to gain benefits of this type of approaches

A new site with Neandertal lineage fossil remains: the Sima I of the Polvorín cave (Karrantza, Biscay, Northern Iberian Peninsula)

We present 16 new human fossil remains found at the Sima I of the Polvorín cave (Karrantza, Northern Iberian Peninsula). The remains probably belong to a single adult gracile individual, that possessed some Neandertal morphological features. The Sima I paleontological site is located in a gallery c. 33 m below the cave entrance. In 2020 we identified 10 human remains in the Arkeologi Museoa (Bilbao). These human remains were found in a purely paleontological assemblage recovered by speleolog..