Terpenic subfraction of Pterodon pubescens induces apoptosis of K562 leukemic cells by modulating gene expression

International audienceDeregulation of cell proliferation and apoptosis is linked to malignant cell development. Leukemia is the most frequent cancer in children, and plants are important sources for new potential anti-cancer agents. Although anti-tumoral effects have been shown for Pterodon pubescens extracts, the mechanisms are still obscure. This study describes in Pterodon pubescens a furane diterpene only reported in Pterodon polygalaeflorus, the methyl-6 alpha-acetoxy-7 beta-hydroxyvouacapan-17 beta-oate, indicated by HRMS and (13)C-NMR analysis, and demonstrates some mechanisms of the anti-leukemia action of its terpene subfraction SF5. SF5 induced cytotoxic and anti-proliferative effects on K562 cells. Increased sub-G1 nuclei and Annexin V(+)-FITC cells confirmed apoptosis of leukemic cells by treatment of these cells with SF5. Down-regulation of DNMT1 gene transcription and over-expression of Apaf-1 mRNA suggested that SF5 may be inducing apoptosis of K562 cells by epigenetic up-regulation of pro-apoptotic proteins involved in the mitochondrial intrinsic pathway

Caracterizacao dos polissacarideos sulfatados das tunicas de diferentes especies de ascidias e determinacao da estrutura da fracao f-1 de Styela plicata

BV UNIFESP: Teses e dissertaçõe

Draft genome sequences of three NDM-1-producing Enterobacteriaceae species isolated from Brazil

Submitted by sandra infurna ([email protected]) on 2015-11-16T10:40:51Z No. of bitstreams: 1 poliana_silveira_etal_IOC_2015.pdf: 225430 bytes, checksum: 42e76966d4a05e4cf0e9c2fe84099cb8 (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2015-11-16T10:54:07Z (GMT) No. of bitstreams: 1 poliana_silveira_etal_IOC_2015.pdf: 225430 bytes, checksum: 42e76966d4a05e4cf0e9c2fe84099cb8 (MD5)Made available in DSpace on 2015-11-16T10:54:07Z (GMT). No. of bitstreams: 1 poliana_silveira_etal_IOC_2015.pdf: 225430 bytes, checksum: 42e76966d4a05e4cf0e9c2fe84099cb8 (MD5) Previous issue date: 2015Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.The emergence of multidrug-resistant Enterobacteriaceae strains producing carbapenemases, such as NDM-1, has become a major public health issue due to a high dissemination capacity and limited treatment options. Here we describe the draft genome of three NDM-1-producing isolates: Providencia rettgeri (CCBH11880), Enterobacter hormaechei subsp. oharae (CCBH10892) and Klebsiella pneumoniae (CCBH13327), isolated in Brazil. Besides blaNDM-1, resistance genes to aminoglycosides [aadA1, aadA2, aac(6’)-Ib-cr] and quinolones (qnrA1, qnrB4) were observed which contributed to the multidrug resistance profile. The element ISAba125 was found associated to the blaNDM-1 gene in all strains

Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents

Submitted by sandra infurna ([email protected]) on 2016-06-23T17:59:29Z No. of bitstreams: 1 milene_oliveira_etal_IOC_2016.pdf: 2331961 bytes, checksum: 5fa155fc65b069851c1f52de01101bfd (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-06-23T18:13:32Z (GMT) No. of bitstreams: 1 milene_oliveira_etal_IOC_2016.pdf: 2331961 bytes, checksum: 5fa155fc65b069851c1f52de01101bfd (MD5)Made available in DSpace on 2016-06-23T18:13:32Z (GMT). No. of bitstreams: 1 milene_oliveira_etal_IOC_2016.pdf: 2331961 bytes, checksum: 5fa155fc65b069851c1f52de01101bfd (MD5) Previous issue date: 2016Submitted by Angelo Silva ([email protected]) on 2016-07-07T11:16:46Z No. of bitstreams: 3 milene_oliveira_etal_IOC_2016.pdf.txt: 28934 bytes, checksum: d36de3026603b493f09fedfb89ef99a0 (MD5) milene_oliveira_etal_IOC_2016.pdf: 2331961 bytes, checksum: 5fa155fc65b069851c1f52de01101bfd (MD5) license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-07-07T11:47:51Z (GMT) No. of bitstreams: 3 license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5) milene_oliveira_etal_IOC_2016.pdf: 2331961 bytes, checksum: 5fa155fc65b069851c1f52de01101bfd (MD5) milene_oliveira_etal_IOC_2016.pdf.txt: 28934 bytes, checksum: d36de3026603b493f09fedfb89ef99a0 (MD5)Made available in DSpace on 2016-07-07T11:47:51Z (GMT). No. of bitstreams: 3 license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5) milene_oliveira_etal_IOC_2016.pdf: 2331961 bytes, checksum: 5fa155fc65b069851c1f52de01101bfd (MD5) milene_oliveira_etal_IOC_2016.pdf.txt: 28934 bytes, checksum: d36de3026603b493f09fedfb89ef99a0 (MD5) Previous issue date: 2016Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Laboratório de Genoma. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.During its life cycle Leishmania spp. face several stress conditions that can cause DNA damages. Base Excision Repair plays an important role in DNA maintenance and it is one of the most conserved mechanisms in all living organisms. DNA repair in trypanosomatids has been reported only for Old World Leishmania species. Here the AP endonuclease from Leishmania (L.) amazonensis was cloned, expressed in Escherichia coli mutants defective on the DNA repair machinery, that were submitted to different stress conditions, showing ability to survive in comparison to the triple null mutant parental strain BW535. Phylogenetic and multiple sequence analyses also confirmed that LAMAP belongs to the AP endonuclease class of proteins