Molecules, dust, and protostars in NGC 3503

Aims. We present here a follow-up study of the molecular gas and dust in the environs of the star forming region NGC 3503. This study aims at dealing with the interaction of the HII region NGC 3503 with its parental molecular cloud, and also with the star formation in the region, that was possibly triggered by the expansion of the ionization front against the parental cloud. Methods. To analyze the molecular gas we use CO(J = 2 → 1), 13CO(J = 2 → 1), C18O(J = 2 → 1), and HCN(J = 3 → 2) line data obtained with the on-the-fly technique from the APEX telescope. To study the distribution of the dust, we make use of unpublished images at 870 μm from the ATLASGAL survey and IRAC-GLIMPSE archival images. We use public 2MASS and WISE data to search for infrared candidate young stellar objects (YSOs) in the region. Results. The new APEX observations allowed the substructure of the molecular gas in the velocity range from ~-28 km s-1 to -23 km s-1 to be imaged in detail. The morphology of the molecular gas close to the nebula, the location of the PDR, and the shape of radio continuum emission suggest that the ionized gas is expanding against its parental cloud, and confirm the champagne flow scenario. We have identified several molecular clumps and determined some of their physical and dynamical properties such as density, excitation temperature, mass, and line width. Clumps adjacent to the ionization front are expected to be affected by the HII region, unlike those that are distant from it. We have compared the physical properties of the two kinds of clumps to investigate how the molecular gas has been affected by the HII region. Clumps adjacent to the ionization fronts of NGC 3503 and/or the bright rimmed cloud SFO 62 have been heated and compressed by the ionized gas, but their line width is not different from those that are too distant from the ionization fronts. We identified several candidate YSOs in the region. Their spatial distribution suggests that stellar formation might have been boosted by the expansion of the nebula. We discard the collect-and-collapse scenario and propose alternative mechanisms such as radiatively driven implosion on pre-existing molecular clumps or small-scale Jeans gravitational instabilities.Facultad de Ciencias Astronómicas y GeofísicasInstituto Argentino de Radioastronomí

A multifrequency study of the active star forming region NGC 6357

To investigate the interaction of the massive stars with the gas and dust in the active star forming region NGC 6357, located in the Sagittarius spiral arm at a distance of 1.7-2.6 kpc (Massey et al. 2001), we analyzed the distribution of the neutral and ionized gas, and that of the dust, based on H, [OIII] and [SII] images obtained with the Curtis-Schmidt telescope at CTIO, radio continuum observations at 1.465 MHz obtained with the Very Large Array (NRAO) in the DnC configuration (synthesized beam = 38), Hi data from the Parkes survey (angular resolution = 15), CO(1-0) observations obtained with the Nanten radiotelescope at Las Campanas Observatory (angular resolution = 2.7), and IR images in the four MSX bands (angular resolution = 18.3). NGC 6357 consists of a low excitation ionized envelope, 50' in diameter, Hll regions in different evolutionary stages (Felli et al. 1990), molecular clouds, OB stars (most of them belonging to the open cluster Pismis 24), and IR sources (Persi et al. 1986). The [Sll]/Ha and [Olll|/Ha line ratios confirm the low excitation of the ionized envelope. Although the Hl emission distribution is dominated by absorption due to radio continuum sources, the optical filaments to the E and W are seen surrounded by neutral gas with velocities in the range -12 to +2 km s-1. The CO emission distribution shows molecular gas associated with the region with velocities in the range -7 to +1 km s-1. A detailed analysis of the region reveals interstellar bubbles and photodissociation regions created by the massive stars in Pi 24 and by undisclosed ionizing sources.Facultad de Ciencias Astronómicas y Geofísica

Effects of T-type calcium channel blockers on cocaine-induced hyperlocomotion and thalamocortical GABAergic abnormalities in mice

Rationale: Repetitive cocaine exposure has been shown to induce GABAergic thalamic alterations. Given the key role of T-type (CaV3) calcium channels in thalamocortical physiology, the direct involvement of these calcium channels in cocaine-mediated effects needs to be further explored. Objective: The objective of this study was to investigate the effect of T-type calcium channel blockers on acute and repetitive cocaine administration that mediates thalamocortical alterations in mice using three different T-type blockers: 2-octanol, nickel, and mibefradil. Methods: During in vitro experiments, whole-cell patch-clamp recordings were conducted in ventrobasal (VB) thalamic neurons from mice treated with acute repetitive cocaine administration (3∈×∈15 mg/kg, i.p., 1 h apart), under bath application of mibefradil (10 μM), 2-octanol (50 μM), or nickel (200 μM). After systemic administration of T-type calcium channel blockers, we evaluated locomotor activity and also recorded GABAergic neurotransmission onto VB neurons in vitro. Results: Bath-applied mibefradil, 2-octanol, or nickel significantly reduced both GABAergic neurotransmission and T-type currents of VB neurons in cocaine-treated mice. In vivo i.p. pre-administration of either mibefradil (20 mg/kg and 5 mg/kg) or 2-octanol (0.5 mg/kg and 0.07 mg/kg) significantly reduced GABAergic mini frequencies onto VB neurons. Moreover, both mibefradil and 2-octanol were able to decrease cocaine-induced hyperlocomotion. Conclusion: The results shown in this study strongly suggest that T-type calcium channels play a key role in cocaine-mediated GABAergic thalamocortical alterations, and further propose T-type channel blockers as potential targets for future pharmacological strategies aimed at treating cocaine's deleterious effects on physiology and behavior.Fil: Bisagno, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Raineri Andersen, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Peskin, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Wikinski, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Uchitel, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Llinás, Rodolfo R.. University of New York; Estados UnidosFil: Urbano Suarez, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentin

Análisis del tratamiento conservador en el paciente con enfermedad renal crónica terminal. Revisión sistemática

Introducción: Desde hace unos años, debido a la inclusión de pacientes con enfermedad renal crónica cada vez más mayores en tratamiento renal sustitutivo, se viene ofreciendo como otra opción, tratamiento renal conservador, con resultados similares en algunas series al tratamiento dialítico. Objetivo: Revisar la literatura científica existente sobre el tratamiento renal conservador en pacientes con enfermedad renal crónica, su supervivencia y calidad de vida. Metodología: Se ha llevado a cabo una revisión sistemática. Se realizó una búsqueda en las bases de datos PubMed, ProQuest, Scielo y Scopus. Se incluyeron artículos científicos en español e inglés, y texto completo disponible. Se analizaron aquellos artículos que trataban sobre pacientes renales en estadío final de la enfermedad renal crónica terminal, tratados con tratamiento paliativo únicamente o en comparación con el tratamiento renal sustitutivo. Resultados: Se han incluido 15 artículos publicados entre los años 2010 y 2020. La enfermedad renal crónica es un problema de alta prevalencia en nuestra población, lo cual condiciona los tratamientos sustitutivos de la función renal. El tratamiento renal conservador surge como opción al sustitutivo, en aquellos pacientes mayores o con una corta expectativa de vida. Como factores más importantes a tener en cuenta surgen la supervivencia y la calidad de vida. Conclusiones: En el paciente con enfermedad renal crónica en tratamiento renal sustitutivo la supervivencia es mayor, aunque con peor calidad de vida, mientras que en el caso del tratamiento renal conservador suele ser al contrario. En pacientes mayores de 75-80 años la supervivencia se iguala, siendo necesario potenciar la calidad de vida y paliar los síntomas de la enfermeda

Molecules, dust, and protostars in NGC 3503

Aims. We present here a follow-up study of the molecular gas and dust in the environs of the star forming region NGC 3503. This study aims at dealing with the interaction of the HII region NGC 3503 with its parental molecular cloud, and also with the star formation in the region, that was possibly triggered by the expansion of the ionization front against the parental cloud. Methods. To analyze the molecular gas we use CO(J = 2 → 1), 13CO(J = 2 → 1), C18O(J = 2 → 1), and HCN(J = 3 → 2) line data obtained with the on-the-fly technique from the APEX telescope. To study the distribution of the dust, we make use of unpublished images at 870 μm from the ATLASGAL survey and IRAC-GLIMPSE archival images. We use public 2MASS and WISE data to search for infrared candidate young stellar objects (YSOs) in the region. Results. The new APEX observations allowed the substructure of the molecular gas in the velocity range from ~-28 km s-1 to -23 km s-1 to be imaged in detail. The morphology of the molecular gas close to the nebula, the location of the PDR, and the shape of radio continuum emission suggest that the ionized gas is expanding against its parental cloud, and confirm the champagne flow scenario. We have identified several molecular clumps and determined some of their physical and dynamical properties such as density, excitation temperature, mass, and line width. Clumps adjacent to the ionization front are expected to be affected by the HII region, unlike those that are distant from it. We have compared the physical properties of the two kinds of clumps to investigate how the molecular gas has been affected by the HII region. Clumps adjacent to the ionization fronts of NGC 3503 and/or the bright rimmed cloud SFO 62 have been heated and compressed by the ionized gas, but their line width is not different from those that are too distant from the ionization fronts. We identified several candidate YSOs in the region. Their spatial distribution suggests that stellar formation might have been boosted by the expansion of the nebula. We discard the collect-and-collapse scenario and propose alternative mechanisms such as radiatively driven implosion on pre-existing molecular clumps or small-scale Jeans gravitational instabilities.Facultad de Ciencias Astronómicas y GeofísicasInstituto Argentino de Radioastronomí

Effects of two different decellularization routes on the mechanical properties of decellularized lungs

Considering the limited number of available lung donors, lung bioengineering using whole lung scaffolds has been proposed as an alternative approach to obtain lungs suitable for transplantation. However, some decellularization protocols can cause alterations on the structure, composition, or mechanical properties of the lung extracellular matrix. Therefore, the aim of this study was to compare the acellular lung mechanical properties when using two different routes through the trachea and pulmonary artery for the decellularization process. This study was performed by using the lungs excised from 30 healthy male C57BL/6 mice, which were divided into 3 groups: tracheal decellularization (TDG), perfusion decellularization (PDG), and control groups (CG). Both decellularized groups were subjected to decellularization protocol with a solution of 1% sodium dodecyl sulfate. The behaviour of mechanical properties of the acellular lungs was measured after decellularization process. Static (Est) and dynamic (Edyn) elastances were obtained by the end-inspiratory occlusion method. TDG and PDG showed reduced Est and Edyn elastances after lung decellularization. Scanning electron microscopy showed no structural changes after lung decellularization of the TDG and PDG. In conclusion, was demonstrated that there is no significant difference in the behaviour of mechanical properties and extracellular matrix of the decellularized lungs by using two different routes through the trachea and pulmonary artery

Agenesis of the right hepatic lobe: an unusual finding in imaging diagnostic exams

Hepatic lobar agenesis is an uncommon condition observed in 1/19000 autopsies and may be more rare in the case of the right lobe, with close to 40 cases reported in the literature. Its diagnosis is based on the non-identification of the hepatic duct, the corresponding portal and hepatic veins. We describe a case of right hepatic lobe agenesis as found in abdominal imaging in a 63-year-old woman with chronic diffuse abdominal discomfort and laboratory findings of a slight elevation of liver enzymes.A agenesia lobar hepática é uma condição incomum, observada em 1/19000 autopsias e podendo ser mais rara em se tratando do lobo direito, com números próximos de 40 casos relatados na literatura. O seu diagnóstico se baseia na não identificação do ducto hepático, das veias hepática e portal correspondentes. Descrevemos um caso de uma agenesia do lobo direito hepático como achado em exames de imagem abdominais em uma mulher de 63 anos com queixa de desconforto abdominal difuso crônico e apresentando laboratorialmente discreta elevação das enzimas hepáticas

NÍVEIS DE ZINCO NA DIETA DE LEITÕES RECÉM-DESMAMADOS SOBRE O PERFIL DE PARÂMETROS SANGÜÍNEOS

adição de diferentes níveis (0, 1.500, 3.000 e 4.500 ppm) de zinco (utilizando como fonte o ZnO) na dieta de leitões influencia o perfil de parâmetros sangüíneos (cálcio, cobre, ferro, zinco, hemoglobina (Hb)), hematócrito (Ht), proteínas séricas totais (PST), albumina (ALB), globulina (GLOB) e atividade enzimática sérica de fosfatase alcalina (FA), aspartato aminotransferase (ASP), alanina aminotransferase (ALT) e gama glutamil transferase (GGT)). Utilizaram-se sessenta leitões machos castrados, mestiços, desmamados aos 21 dias de idade, procedentes de sistema de criação em sítios separados, dentro dos períodos: Período 1 (P1), do desmame (dia zero) até 14 dias pós-desmame; Período 2 (P2), de 15 a 42 dias pós-desmame e Período Total (PT), do desmame aos 42 dias pós-desmame. As coletas de sangue foram efetuadas aos 0, 7, 14, 21 e 42 dias pós-desmame. Utilizou-se o DIC, num esquema em parcelas subdivididas, tendo como parcelas os quatro níveis de Zn nas rações, e como subparcelas os dias de coleta. Concluiu-se que a adição de 4.500 ppm de Zn por duas semanas pós-desmame pode elevar o nível sérico de Zn. Os parâmetros sangüíneos sofreram efeito do dia de coleta. PALAVRAS-CHAVES: Leitões desmamados, óxido de zinco, suínos

hnRNP A1 and hnRNP F Modulate the Alternative Splicing of Exon 11 of the Insulin Receptor Gene

Exon 11 of the insulin receptor gene (INSR) is alternatively spliced in a developmentally and tissue-specific manner. Linker scanning mutations in a 5′ GA-rich enhancer in intron 10 identified AGGGA sequences that are important for enhancer function. Using RNA-affinity purification and mass spectrometry, we identified hnRNP F and hnRNP A1 binding to these AGGGA sites and also to similar motifs at the 3′ end of the intron. The hnRNPs have opposite functional effects with hnRNP F promoting and hnRNP A1 inhibiting exon 11 inclusion, and deletion of the GA-rich elements eliminates both effects. We also observed specific binding of hnRNP A1 to the 5′ splice site of intron 11. The SR protein SRSF1 (SF2/ASF) co-purified on the GA-rich enhancer and, interestingly, also competes with hnRNP A1 for binding to the splice site. A point mutation -3U→C decreases hnRNP A1 binding, increases SRSF1 binding and renders the exon constitutive. Lastly, our data point to a functional interaction between hnRNP F and SRSF1 as a mutant that eliminates SRSF1 binding to exon 11, or a SRSF1 knockdown, which prevents the stimulatory effect of hnRNP F over expression

Espaços e paisagens: Antiguidade Clássica e Heranças Contemporâneas vol. II

Actas do VII congresso internacional da Associação Portuguesa de Estudos Clássicos, vol. 2º., dedicado ao estudo da recepção dos temas «Espaços» e «Paisagens». O volume conta com os seguintes ensaios: 1)Interpelações entre espaço e paisagem: uma leitura das Confissões de Agostinho (Teresa Santos); 2)Espaço e fronteiras do mundo romano na Antiguidade Tardia.Continuidade e rupturas em relação à Europa Actual (Paula Barata Dias); 3)El paisaje en la Peregrinatio Egeriae (Ana Isabel Martín Ferreira); 4)Espaço e alegoria na poesia épica portuguesa seiscentista (Manuel dos Santos Rodrigues); 5)A mundividência de Diogo Pires à luz da colectânea poética dos Xenia (António Manuel Lopes Andrade); 6) Espaço literário feminino. A obra de Maria de Mesquita Pimentel (Antónia Fialho Conde); 7)Paisaje, clima y carácter en De humana physiognomonia de Giovan Battista della Porta (Miguel Ángel González Manjarrés); 8) Espaços para o dever e o lazer num modelo de educação humanística (1599)(Margarida Miranda); 9)Utopía, espacios soñados y Mito Clásico en la Tragicomedia de Los Jardines y Los Campos Sabeos de Feliciana Enríquez de Guzmán (Cristina de la Rosa Cubo); 10)O espaço físico como alegoria da tragédia humana. Concepção do espaço dramático na Trilogia de Édipo de J. de Castro Osório (Ália Rosa C. Rodrigues); 11) Releituras de um passado grego: a tragédia Oedipus Tyrannos de Sófocles (Rogério José de Souza); 12)Uma velha África: Heródoto e o ensino de História da África (José Maria Gomes de Souza Neto); 13)Elaboração da luz no espaço entre a igreja visível e invisível no pensamentode Kant. As raízes platónicas e utópicas do modelo original (Giovanni Panno); 14)Descobrir com jovens: espaços e paisagens do Truculentus de Plauto (Adriano Milho Cordeiro); 15)Reflexos do espaço de exílio ovidiano no Livro do Desassossego (Rodolfo Pais Nunes Lopes); 16)Ambiência clássica em invectivas às ditaduras militar e salazarista (Carlos Morais); 17)O mundo clássico nas Vidas Apócrifas de Amadeu Lopes Sabino:alguns paralelos imaginados (Glaucianne Silva dos Santos Heuer); 18) Observação filosófica e contemplação poética das paisagens em Lucrécio (Andrés Pociña); 19)O mito de Orpheus. A plasticidade do mito nas vozes de Virgílio,Vinícius e Camus (Elaine C. Prado dos Santos); 20)Espaço e paisagem em Doze Naus de Manuel Alegre (José Ribeiro Ferreira)