HLA-DM: An in vivo facilitator of MHC class II peptide loading

AbstractInitiation of cell-mediated immune responses requires processing of intracellular pathogens into peptide fragments and presentation of these fragments to effector T cells. In the past few years, considerable progress has been made in understanding the molecular mechanisms underlying antigen processing and presentation. In particular, recent work has led to an understanding of the function of HLA-DM (DM), a key player in antigen presentation, whose importance was first appreciated only 1 year ago

Transmitting Biological Waveforms Using a Cellular Phone

There exists a need to remotely monitor fully mobile patients in their natural environments. Monitoring a patient's biological waveforms can track a patient's vital signs or facilitate the diagnosis of a disease, which could then be treated to help prolong and/or improve the subject's life. If a patient must be monitored without the delay associated with delivering data stored on a recording device, biotelemetry is necessary. Biotelemetry entails transmitting biological waveforms to a remote site for recording, processing and analysis. Due to the limitations of the currently popular methods of biotelemetry, this thesis proposes the use of the increasingly prevalent cellular phone system. An adaptor design is developed to facilitate biotelemetry utilizing the most common features of a cell phone, barring the need for cell phone modification, as required for affordability. As cell phones notoriously confound sensitive medical equipment, especially patient-connected devices, their use is often distanced from sensitive equipment. However, the desire to use cell phones to transmit biological waveforms requires their joint-proximity to patient-connected devices. The adaptor must amplify the waveforms while rejecting cell phone interference to achieve an adequate signal-to-noise ratio. As the frequency range of most biological data does not conform to the passband of the phone system, the adapter must modulate the biological data. To limit the adapter's size and weight, this design exploits the cell phone's battery power. Methods are also introduced to receive and reconstruct high-fidelity representations of the original biological waveform

Trajectory Mapping and Applications to Data from the Upper Atmosphere Research Satellite

The problem of creating synoptic maps from asynoptically gathered trace gas data has prompted the development of a number of schemes. Most notable among these schemes are the Kalman filter, the Salby-Fourier technique, and constituent reconstruction. This paper explores a new technique called trajectory mapping. Trajectory mapping creates synoptic maps from asynoptically gathered data by advecting measurements backward or forward in time using analyzed wind fields. A significant portion of this work is devoted to an analysis of errors in synoptic trajectory maps associated with the calculation of individual parcel trajectories. In particular, we have considered (1) calculational errors; (2) uncertainties in the values and locations of constituent measurements, (3) errors incurred by neglecting diabatic effects, and (4) sensitivity to differences in wind field analyses. These studies reveal that the global fields derived from the advection of large numbers of measurements are relatively insensitive to the errors in the individual trajectories. The trajectory mapping technique has been successfully applied to a variety of problems. In this paper, the following two applications demonstrate the usefulness of the technique: an analysis of dynamical wave-breaking events and an examination of Upper Atmosphere Research Satellite data accuracy

A Deep Multicolor Survey. VI. Near-Infrared Observations, Selection Effects, and Number Counts

I present near-infrared J (1.25um), H (1.65um), and K (2.2um) imaging observations of 185 square arcminutes in 21 high galactic latitude fields. These observations reach limiting magnitudes of J ~ 21 mag, H ~ 20 mag and K ~ 18.5 mag. The detection efficiency, photometric accuracy and selection biases as a function of integrated object brightness, size, and profile shape are quantified in detail. I evaluate several popular methods for measuring the integrated light of faint galaxies and show that only aperture magnitudes provide an unbiased measure of the integrated light that is independent of apparent magnitude. These J, H, and K counts and near-infrared colors are in best agreement with passive galaxy formation models with at most a small amount of merging (for Omega_M = 0.3, Omega_Lambda = 0.7).Comment: AJ Accepted (Feb 2001). 28 pages, 7 embedded ps figures, AASTEX5. Minor changes to submitted version. Also available at http://www.astronomy.ohio-state.edu/~martini/pubs

Ligation of MHC Class II Induces PKC-Dependent Clathrin-Mediated Endocytosis of MHC Class II

In addition to antigen presentation to CD4(+)T cells, aggregation of cell surface major histocompatibility complex class II (MHC-II) molecules induces signal transduction in antigen presenting cells that regulate cellular functions. We previously reported that crosslinking of MHC-II induced the endocytosis of MHC-II, which was associated with decreased surface expression levels in murine dendritic cells (DCs) and resulted in impaired activation of CD4(+)T cells. However, the downstream signal that induces MHC-II endocytosis remains to be elucidated. In this study, we found that the crosslinking of MHC-II induced intracellular Ca(2+)mobilization, which was necessary for crosslinking-induced MHC-II endocytosis. We also found that these events were suppressed by inhibitors of Syk and phospholipase C (PLC). Treatments with a phorbol ester promoted MHC-II endocytosis, whereas inhibitors of protein kinase C (PKC) suppressed crosslinking-induced endocytosis of MHC-II. These results suggest that PKC could be involved in this process. Furthermore, crosslinking-induced MHC-II endocytosis was suppressed by inhibitors of clathrin-dependent endocytosis. Our results indicate that the crosslinking of MHC-II could stimulate Ca(2+)mobilization and induce the clathrin-dependent endocytosis of MHC-II in murine DCs

Fusion of Dilute ALA_L Lattice Models

The fusion procedure is implemented for the dilute $A_L$ lattice models and a fusion hierarchy of functional equations with an $su(3)$ structure is derived for the fused transfer matrices. We also present the Bethe ansatz equations for the dilute $A_L$ lattice models and discuss their connection with the fusion hierarchy. The solution of the fusion hierarchy for the eigenvalue spectra of the dilute $A_L$ lattice models will be presented in a subsequent paper.Comment: 45 pages; Latex file; epsf.tex needed for graph

Quasar Candidates in the Hubble Deep Field

We focus on the search for unresolved faint quasars and AGN in the crude combine images using a multicolor imaging analysis that has proven very successful in recent years. Quasar selection was carried out both in multicolor space and in "profile space," defined as the multi-parameter space formed by the radial profiles of the objects in the different images. By combining the dither frames available for each filter, we were able to obtain well-sampled radial profiles of the objects and measure their deviation from that of a stellar source. We also generated synthetic quasar spectra in the range 1.0 < z < 5.5 and computed expected quasar colors. We determined that the data are 90% complete for point sources at 26.2, 28.0, 27.8, 26.8 in the F300W, F450W, F606W and F814W filters, respectively. We find 41 compact objects in the HDF: 8 pointlike objects with colors consistent with quasars or stars, 18 stars, and 15 slightly resolved objects, 12 of which have colors consistent with quasars or stars. We estimate the upper limit of unresolved and slightly resolved quasars/AGNs with V < 27.0 and z < 3.5 to be 20 objects (16,200 per deg^2). We find good agreement among authors on the number of stars and the lack of quasar candidates with z > 3.5. We find more quasar candidates than previous work because of our more extensive modeling and use of all of the available color information. (abridged)Comment: We have clarified our discussion and conclusions, added some references and removed the appendix, which is now available from the first author. 37 pages including 10 embedded postscript figures and 6 tables. To appear in the Feb. 99 issue of A

Geometrical structure effect on localization length of carbon nanotubes

The localization length and density of states of carbon nanotubes are evaluated within the tight-binding approximation. By comparison with the corresponding results for the square lattice tubes, it is found that the hexagonal structure affects strongly the behaviors of the density of states and localization lengths of carbon nanotubes.Comment: 7 pages, 4 figures, revised version to appear in Chin. Phys. Lett. The title is changed. Some arguments are adde