Análisis comparativo de células madre mesenquimales procedentes de pacientes artrósicos y sanos en diferenciación condrogénica

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Targeted Phospholipidomic Analysis of Synovial Fluid as a Tool for Osteoarthritis Deep Phenotyping

[Abstract] Objective. The aim of this study was to carry out a targeted phospholipidomic analysis on synovial fluid (SF) from patients with different grades of osteoarthritis (OA) and controls, in order to search for specific phospholipid profiles that may be useful for the deep phenotyping of this disease. Design. Multiple reaction monitoring-mass spectrometry (MRM/MS) was applied to explore the potential phospholipidomic differences in the SF of knee OA patients (n ​= ​15) (subclassified into early- and late-stage OA) and non-OA controls (n ​= ​4). Multivariate statistical analyses conducted by partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) were performed to identify significantly altered phospholipids in OA, characterize phospholipidomic profiles associated with the radiographic stage of the disease and describe potential endotypes at early stages. Results. Significant discrimination of phospholipid profiles between non-OA controls and the early- and late-stage OA groups were found by PLS-DA and HCA. Compared to SF from non-OA controls, OA patients showed higher levels of most quantified phospholipid species, including phosphatidylcholines (PC), phosphatidylserines and phosphatidylinositols. Furthermore, several PC species showed significant differences in abundance between the two OA subgroups and were negatively correlated with cartilage damage. Finally, two distinct endotypes of early-stage OA were identified based on the phospholipidomic profile of SF. Conclusions. Our data provides a novel insight into the phospholipid profiles of OA synovial fluid, revealing specific alterations associated with the radiographic stage of the disease. This targeted phospholipidomic profiling also facilitated the characterization of two different OA endotypes at early stages of the disease.This work is supported by grants from Fondo Investigación Sanitaria-Spain (PI16/02124, PI17/00404, PI19/01206, PI20/00793 and RETIC-RIER-RD16/0012/0002), integrated in the National Plan for Scientific Program, Development and Technological Innovation 2013–2016 and funded by the ISCIII-General Subdirection of Assessment and Promotion of Research - European Regional Development Fund (FEDER) “A way of making Europe”. This study is also supported by AE CICA-INIBIC (ED431E 2018/03) and grants IN607A 2017/11, IN607A 2021/7 and IN607D 2020/10 from Axencia Galega de Innovacion - Xunta de Galicia. The Biomedical Research Networking Center (CIBER) is an initiative from Instituto de Salud Carlos III (ISCIII). The Proteomics Unit of GIR belongs to ProteoRed, PRB3- ISCIII (PT17/0019/0014)Xunta de Galicia; ED431E 2018/03Xunta de Galicia; IN607A 2017/11Xunta de Galicia; IN607A 2021/7Xunta de Galicia; IN607D 2020/1

Estudio comparativo del secretoma de condrocitos articulares humanos analizados mediante las técnicas iTRAQ e SILAC

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Cuantificación por SILAC del efecto de la nicotina sobre condrocitos articulares

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Quantitative proteomics analysis of chondrogenic differentiation of mesenchymal stem cells by SILAC

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A combination of both proteomic and genetic approach to indentify de-regulated pathway in progeria, an accelerated aging disorder

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Variable effect of 3 different chondroitin sulfate compounds on chondrocytes secreted proteins profile revealed by silac techinique

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Prevalencia de diabetes tipo 2 e hipertensión arterial en adultos de nivel económico bajo de Monterrey, México

Antecedentes: la hiperglucemia es signo característico de un grupo enfermedades, la diabetes tipo 2 abarca del 90 al 95% de los casos, y provoca alta morbilidad, e incapacidad e incluso muerte prematura. Objetivo: determinar la prevalencia de diabetes mellitus tipo 2, prediabetes y la de HA en adultos en área urbana de bajo nivel económico en Monterrey, México. Método: estudio de corte transversal con selección aleatoria de adultos (hombres y mujeres no embarazadas ni lactando) en una comunidad de bajo nivel económico. Se obtuvo glucemia capilar y plasmática en ayuno y 2h-poscarga oral de 75 g glucosa. Diagnósticos según criterios del American Diabetes Association 2004. Se presenta prevalencia con intervalo de confianza (IC95%) y regresión múltiple en inferencia de factores. Resultados: la prevalencia de diabetes mellitus tipo 2 fue 14.1% (9.6-18.6%), prediabetes 12.8% (8.5-17.1) y de hipertensión arterial 26.24% (20.4-32.4%). La edad, el índice de masa corporal y género justifican 38% de la variación de la hipertensión arterial (r = 0.616, todas p < 0.001); pero sólo 5% (r = 0.23) de la variación glucémica fue explicado por la edad (p < 0.01) y el índice de masa corporal (p < 0.05). Conclusiones: la prevalencia de hipertensión arterial fue la esperada, pero la de diabetes mellitus 2 sugiere ser más alta que en reportes previos. En esta población la adiposidad no justifica la alta prevalencia de diabetes mellitus 2, ni de prediabetes. ABSTRACT Background: Hyperglycemia is a characteristic sign of several diseases. In the case of type 2 diabetes, it has an effect on 90 to 95% of the patients. It produces a high morbidity, incapacity and even death. Objective: To determine the prevalence of type 2 diabetes mellitus, pre-diabetes and hypertension in a low-income urban area in Monterrey, Mexico. Method: A random, cross-sectional study was done in a low-income urban population in Monterrey, Mexico. Patients who had participated in a baseline glycemic study from 1992 to 1993 were re-examined. The concentration of capillary and plasmatic glucose on fasting and two hour post-load oral glucose (75 g) were determined. The diagnoses were established following the criteria of the American Diabetes Association 2004. Multiple regression analyses were done to infer the factors related with glycemia; the confidence interval was 95%. Results: The type 2 diabetes mellitus prevalence was 14.1%, pre-diabetes 12.8% and the one for hypertension was 26.24%. Age, body mass index and gender prognosticated in an independent manner the values of hypertension (p < 0.001). Glycemia was prognosticated based on age (p < 0.01) and body mass index (p < 0.05). Conclusions: Prevalence of type 2 diabetes was higher in low-income adult patients, but hypertension prevalence remained within normal parameters. In this population, the body mass index did not justify the high prevalence of type 2 diabetes nor that of pre-diabetes