Sustainability analysis of small ruminant farms involved in the conservation of natural resources

pendiente autorización de la revistaThe Environment Department of the Government of Andalusia (Spain) has recently assumed the responsibility of integrating extensive livestock grazing as a tool for fire prevention. The present study aims to analyse the sustainability of 60 farms (mainly small ruminant farms) belonging to the Andalusian Network of Grazed Fuel breaks. Farms are located mainly in Natural Areas of Eastern Andalusia. 9291 goats, 24491 ewes and 170 cows grazed in 260 km of fuel breaks. 27 farms have only sheep (mainly meat purpose), 18 only goats (mainly dairy purpose), 10 goat and sheep, 3 small ruminants and suckling cows and 2 small ruminants, suckling cows and Iberian pigs; only 4 goat farms produce cheese. Meat and milk production has a marked seasonal variation. The feed supplied in the manger is relatively high, especially in goat herds. Hard work, small farm profitability and lack of assurance of farm continuity are the most important aspects that negatively affect the sustainability of these farms. For improving sustainability, farmers are to be able to exploit strengths, absorb shocks, adjust following a disturbance and transform their farm to take advantage of new opportunities.Le Département de l’Environnement du Gouvernement de l'Andalousie (Espagne) a récemment pris la responsabilité d’intégrer le pâturage extensif dans la prévention des incendies. La présente étude analyse la viabilité de 60 exploitations (surtout des petites exploitations de ruminants) qui appartiennent au Réseau Andalou des Exploitations Collaboratrices au Pâturage des Coupe-feux. Les fermes sont situées principalement dans les Parcs Naturels de l'Est de l'Andalousie. 9291 chèvres, 24491 brebis et 170 vaches sont en pâturage dans 260 km de coupe-feux. 27 exploitations ont seulement des ovins (surtout d’aptitude viande), 18 ont seulement des caprins (surtout d’aptitude laitière), 10 ont des ovins et caprins, 3 ont des petits ruminants et des bovins et 2 ont des petits ruminants, des bovins et des porcins de la race Ibérique ; il n’y a que 4 fermes qui fabriquent du fromage. La production de viande et de lait sont très saisonnières. Les apports d'aliments à l’auge sont relativement élevés, en particulier dans le cas des chèvres. Les aspects plus négativement influents sur la durabilité des exploitations sont: la pénibilité du travail, la faible rentabilité des petites exploitations et le manque d'assurance de la continuité des exploitations. Pour améliorer la durabilité des ces exploitations, les éleveurs devraient valoriser les points forts des systèmes d’exploitation, résister aux difficultés, faire des ajustements après une perturbation du système et faire des changements dans les exploitations afin de profiter des opportunités

Wnt/β-Catenin Signaling Contributes to Paclitaxel Resistance in Bladder Cancer Cells with Cancer Stem Cell-Like Properties

The Wnt/β-catenin pathway plays an important role in tumor progression and chemother apy resistance and seems to be essential for the maintenance of cancer stem cells (CSC) in several tumor types. However, the interplay of these factors has not been fully addressed in bladder cancer. Here, our goal was to analyze the role of the Wnt/β-catenin pathway in paclitaxel resistance and to study the therapeutic efficacy of its inhibition in bladder cancer cells, as well as to determine its influence in the maintenance of the CSC-like phenotype in bladder cancer. Our results show that paclitaxel-resistant HT1197 cells have hyperactivation of the Wnt/β-catenin pathway and increased CSC-like properties compared with paclitaxel-sensitive 5637 cells. Paclitaxel sensitivity diminishes in 5637 cells after β-catenin overexpression or when they are grown as tumorspheres, enriched for the CSC-like phenotype. Additionally, downregulation of β-catenin or inhibition with XAV939 sensitizes HT1197 cells to paclitaxel. Moreover, a subset of muscle-invasive bladder carcinomas shows aberrant expression of β-catenin that associates with positive expression of the CSC marker ALDH1A1. In conclusion, we demonstrate that Wnt/β-catenin signaling contributes to paclitaxel resistance in bladder cancer cells with CSC-like properties.Instituto de Salud Carlos III FIS-PI17/1240Instituto de Salud Carlos III FIS-PI20/1641Ministry of Economy, Industry and Competitiveness SAF2017-87358-C2-1-R and -2-RMinisterio de Ciencia e Innovación PID2020-118774RB-C21 and -C22Consejería de Salud y Familias PI-0213-2020Consejería de Salud y Familias OH-0017-201

Obatoclax and Paclitaxel Synergistically Induce Apoptosis and Overcome Paclitaxel Resistance in Urothelial Cancer Cells

Paclitaxel is a treatment option for advanced or metastatic bladder cancer after the failure of first-line cisplatin and gemcitabine, although resistance limits its clinical benefits. Mcl-1 is an anti-apoptotic protein that promotes resistance to paclitaxel in different tumors. Obatoclax, a BH3 mimetic of the Bcl-2 family of proteins, antagonizes Mcl-1 and hence may reverse paclitaxel resistance in Mcl-1-overexpressing tumors. In this study, paclitaxel-sensitive 5637 and -resistant HT1197 bladder cancer cells were treated with paclitaxel, obatoclax, or combinations of both. Apoptosis, cell cycle, and autophagy were measured by Western blot, flow cytometry, and fluorescence microscopy. Moreover, Mcl-1 expression was analyzed by immunohistochemistry in bladder carcinoma tissues. Our results confirmed that paclitaxel alone induced Mcl-1 downregulation and apoptosis in 5637, but not in HT1197 cells; however, combinations of obatoclax and paclitaxel sensitized HT1197 cells to the treatment. In obatoclax-treated 5637 and obatoclax + paclitaxel-treated HT1197 cells, the blockade of the autophagic flux correlated with apoptosis and was associated with caspase-dependent cleavage of beclin-1. Obatoclax alone delayed the cell cycle in 5637, but not in HT1197 cells, whereas combinations of both retarded the cell cycle and reduced mitotic slippage. In conclusion, obatoclax sensitizes HT1197 cells to paclitaxel-induced apoptosis through the blockade of the autophagic flux and effects on the cell cycle. Furthermore, Mcl-1 is overexpressed in many invasive bladder carcinomas, and it is related to tumor progression, so Mcl-1 expression may be of predictive value in bladder cancer.España, Sistema Público Andaluz Biobanco y ISCIII-Red de Biobancos PT17/0015/004

Ruta de avistamiento punta caribana

Se presenta la propuesta ecotúristica denominada ruta de avistamiento Punta Caribana resultado de los procesos de investigación del Complejo Tecnológico Agroindustrial, Pecuario y Turístico del SENA Urabá, en el marco de la convocatoria Colciencias ideas para el cambio del proyecto Plataforma de avistamiento para tortugas en la playa Bobalito, Lechugal, Necoclí – Antioquia, utilizando iluminación especial y energía alternativa: propuesta de manejo y conservación.The ecotourism proposal called the Punta Caribana sighting route is presented as a result of the research processes of the Agroindustrial, Livestock and Tourism Technology Complex of SENA Urabá, within the framework of the Colciencias call for ideas for the change of the project. Turtle watching platform on the beach Bobalito, Lechugal, Necoclí - Antioquia, using special lighting and alternative energy: management and conservation proposal.Presentación -- Ruta de avistamiento Punta Caribana -- Asociación ACAETUR -- ¿Quiénes somos? -- Objetivos -- Valores corporativos -- Nuestra historia -- Guías locales -- Ubicación geográfica de la playa el Bobalito en la vereda Lechugal -- Recorrido inicial “Caminando te contamos nuestra historia” -- “Recorrido guiado en la plataforma de avistamiento de tortugas marinas” Bienvenida -- Actividades de conservación y preservación ¡Yo quiero ser voluntario! -- Ruta # 1 Cerro del Águila “caminata ecológica” -- Ruta # 2 Recorrido guiado “Ciénagas Marimonda y Salado” -- Ruta # 3 Estuario natural “Ensenada de Rionegro” -- Ruta # 4 Volcán de lodo “Madre Selva” -- Gastronomía local -- Recomendaciones -- Especificaciones del paquete -- Agradecimientos 16. Bibliografíana19 página

Ruta de avistamiento punta caribana

Se presenta la propuesta ecotúristica denominada ruta de avistamiento Punta Caribana resultado de los procesos de investigación del Complejo Tecnológico Agroindustrial, Pecuario y Turístico del SENA Urabá, en el marco de la convocatoria Colciencias ideas para el cambio del proyecto Plataforma de avistamiento para tortugas en la playa Bobalito, Lechugal, Necoclí – Antioquia, utilizando iluminación especial y energía alternativa: propuesta de manejo y conservación.The ecotourism proposal called the Punta Caribana sighting route is presented as a result of the research processes of the Agroindustrial, Livestock and Tourism Technology Complex of SENA Urabá, within the framework of the Colciencias call for ideas for the change of the project. Turtle watching platform on the beach Bobalito, Lechugal, Necoclí - Antioquia, using special lighting and alternative energy: management and conservation proposal.Presentación -- Ruta de avistamiento Punta Caribana -- Asociación ACAETUR -- ¿Quiénes somos? -- Objetivos -- Valores corporativos -- Nuestra historia -- Guías locales -- Ubicación geográfica de la playa el Bobalito en la vereda Lechugal -- Recorrido inicial “Caminando te contamos nuestra historia” -- “Recorrido guiado en la plataforma de avistamiento de tortugas marinas” Bienvenida -- Actividades de conservación y preservación ¡Yo quiero ser voluntario! -- Ruta # 1 Cerro del Águila “caminata ecológica” -- Ruta # 2 Recorrido guiado “Ciénagas Marimonda y Salado” -- Ruta # 3 Estuario natural “Ensenada de Rionegro” -- Ruta # 4 Volcán de lodo “Madre Selva” -- Gastronomía local -- Recomendaciones -- Especificaciones del paquete -- Agradecimientos 16. Bibliografíana19 página

Los sonidos de un majestuoso paisaje escondido.

En la presente investigación se llevó a cabo un proyecto en donde se desarrolla un programa radial online compuesto por tres capítulos, con los cuales se transporte por medio de la imaginación a los estudiantes de segundo semestre de comunicación social de la Universidad Minuto de Dios Centro Regional Soacha, para que por medio de sonidos reales conozcan el corregimiento número uno de Soacha, específicamente las veredas San Jorge y Hungría. Lo anterior se logró realizar gracias a que por medio de la escucha se puede conseguir que un radio oyente se sumerja en un mundo desconocido para él y logre con ello experimentar sensaciones diferentes a las que tiene en su diario vivir, provocando que nazca la necesidad de conocer y tener frente a frente lo que está escuchando

Phase angle and COVID-19: a systematic review with meta-analysis

Phase angle (PhA) has been identified as a poor prognostic factor in patients with COVID-19. This study aimed to achieve a systematic review, where we discussed the potential role of PhA value as a prognostic marker of adverse clinical outcomes such as mortality and complication in hospitalized with SARS-CoV2 infection and established the strength of recommendations for use. A systematic literature review with meta-analysis was done in the main electronic databases from 2020 to January 2023. The selected articles had to investigate adverse consequences of the COVID-19 population and raw bioimpedance parameters such as PhA and published in peer-reviewed journals. GRADE tools regarded the quality of the methodology. The review protocol was registered in PROSPERO. Only eight studies, 483 studies, were eligible for the analysis. In general, differences in PhA were seen between the comparative study groups. Patients with a low PhA experienced poor outcomes. A low PhA was associated with a significantly increased mortality risk [RR: 2.44; 95% CI (1.20–4.99), p = 0.01; I2 = 79% (p = 0.0008)] and higher complications risk [OR: 3.47, 95% CI (1.16 – 10.37), p = 0.03; I2 = 82% (p = 0.004)] in COVID-19 patients. Our analysis showed four evidence-based recommendations on the prognostic value of PhA with two strong recommendations, one of moderate and another of low-moderate quality, for predicting mortality and complications, respectively. We recommend using PhA as a prognostic marker for mortality and complications in this population. Although the results are promising, future studies must identify the PhA cut-off to guide therapeutic decisions more precisely.Funding for open access publishing: Universidad Málaga/CBUA. I.C.-P. was the recipient of a postdoctoral grant (Río Hortega CM 17/00169) and is now the recipient of a postdoctoral grant (Juan Rodes JR 19/00054) from the Instituto de Salud Carlos III and co-funded by Fondo Europeo de Desarrollo Regional-FEDER. Funding for open access charge: Universidad de Málaga / CBU

Rice and bean AHL-mimic quorum-sensing signals specifically interfere with the capacity to form biofilms by plant-associated bacteria

Many bacteria regulate their gene expression in response to changes in their population density in a process called quorum sensing (QS), which involves communication between cells mediated by small diffusible signal molecules termed autoinducers. n-acyl-homoserine-lactones (AHLs) are the most common autoinducers in proteobacteria. QS-regulated genes are involved in complex interactions between bacteria of the same or different species and even with some eukaryotic organisms. Eukaryotes, including plants, can interfere with bacterial QS systems by synthesizing molecules that interfere with bacterial QS systems. In this work, the presence of AHL-mimic QS molecules in diverse Oryza sativa (rice) and Phaseolus vulgaris (bean) plant-samples were detected employing three biosensor strains. A more intensive analysis using biosensors carrying the lactonase enzyme showed that bean and rice seed-extract contain molecules that lack the typical lactone ring of AHLs. Interestingly, these molecules specifically alter the QS-regulated biofilm formation of two plant-associated bacteria, Sinorhizobium fredii SMH12 and Pantoea ananatis AMG501, suggesting that plants are able to enhance or to inhibit the bacterial QS systems depending on the bacterial strain. Further studies would contribute to a better understanding of plant–bacteria relationships at the molecular level

Análisis del papel de la elección en la base de datos reticular observacional en la evaluación de modelos climáticos regionales

Ponencia presentada en: VIII Congreso de la Asociación Española de Climatología celebrado en Salamanca entre el 25 y el 28 de septiembre de 2012.[ES]En este trabajo se presentan los resultados de la evaluación de la habilidad de todos los miembros de dos conjuntos de simulaciones regionales climáticas para la reproducción de la precipitación y temperaturas máximas y mínimas utilizando tres bases de datos reticulares diferentes (SPAIN02, AEMET y E-OBS), que tienen una resolución espacial similar (aprox 25 km). El primer conjunto es multifísica (30 km) y consta de ocho miembros. El segundo conjunto es multimodelo (ESCENA) y consta de 5 miembros (25 km de resolución). Los resultados indican que la bondad de los miembros del conjunto en la reproducción de la climatología observada sobre España depende de las base de datos seleccionada.[EN]This paper presents the results of the skill evaluation of the members of two ensembles of regional climate simulations in reproducing precipitation and maximum and minimum temperatures using three different grid databases (SPAIN02, AEMET and E-OBS ), which have similar spatial resolution (approximately 25 km). The first set is multiphysics (30 km) and consists of eight members. The second set is multi-model (ESCENA) and consists of 5 members (25 km resolution). The results indicate that the skill of the ensemble members in reproducing the observed climate over Spain depends on the selected database

Factores de riesgo cardiovascular: desde los clásicos hasta los emergentes a la luz de las nuevas evidencias

Cardiovascular diseases (CVD) are the main cause of morbidity and mortality worldwide, especially affecting the population over 60 years of age, they can manifest depending on their origin in coronary disease (acute myocardial infarction, angina pectoris, sudden cardiac arrest, heart failure and sudden death) and cerebrovascular disease (strokes and transient ischemic events). Several risk factors associated with the development of CVD have been described, the classic factors (modifiable and non-modifiable), and others that have been added to the list as a result of new evidence, such as inflammation, thrombosis, stress, alterations in the levels of natriuretic peptides, heavy metals/environmental pollutants, renal insufficiency, and some infectious agents. The objective of this article is to present the state of the art associated with classic cardiovascular risk factors (CRFs) and the evidence that supports emerging CRFs.  Las enfermedades cardiovasculares (ECV) son la causa principal de morbimortalidad a nivel mundial, afectando especialmente a la población mayor de 60 años, pueden manifestarse según su origen en enfermedad coronaria (infarto agudo de miocardio, angina pectoris, insuficiencia cardiaca, y muerte súbita) y enfermedad cerebrovascular (accidentes cerebrovasculares y eventos isquémicos transitorios). Varios factores de riesgo asociados al desarrollo de las ECV han sido descritos; los factores clásicos (modificables y no modificables), y otros que se han añadido a la lista como producto de las nuevas evidencias como es el caso de la inflamación, trombosis, estrés, alteraciones en los niveles de péptidos natriuréticos, metales pesados/contaminantes ambientales, insuficiencia renal y algunos agentes infecciosos. El objetivo de este artículo es presentar el estado del arte asociado con los factores de riesgo cardiovasculares (FRC) clásicos y las evidencias que soportan los FRC emergentes. &nbsp