A VACCINE PROTOTYPE USING BACULOVIRUS EXPRESSION SYSTEM FOR THE CONTROL OF AVIAN INFLUENZA VIRUS

A clade 1 sequence of H5 haemaglutinin from an Asian Avian H5N1 isolate was used as a the template to chemically synthetize a codon optimized version for expression in insect cells. A single clone was chosen for expression optimization and changes were introduced in order to maximize the amount of protein to be produced and to resemble another sequence demonstrated to be present in an isolate causing disease in Humans. Preliminary analysis at lab scale have shown promising yields of the haemaglutinin using an activity titration assay and an ELISA-based detection method. Optimization of the cell seeding, MOI, and time of harvest have provided valuable data to ensure sufficient production of the protein compatible with scale up application. In order to test the biological activity of the expressed protein and its ability to trigger an immune response, oil/water emulsions of different amounts of the protein were administered to SPF chickens and antibodies levels were detected using an ELISA-based system and HI titration. Both approaches were able to demonstrate seroconvertion and a dose-response curve was observed among the different doses. Altogether, results support the feasibility of the genetic contruct and the expression platform to produce bulk amounts of protein which could be used for different purposes

Silver-Derived Antimicrobial Coatings for the Prevention of Microbial Biofilms in Metal Pipes

The authors of this study would like to acknowledge the financial and technical support of the CETAQUA Foundation; without the said support, this work would not have been possible.The datasets generated during the current study are available from the corresponding author on reasonable requeBiodeterioration is one of the most important processes in metal pipeline corrosion, and it can be due to physical, chemical, and biological factors. Coatings rich in silver have been used to inhibit this undesirable phenomenon. In this study, the antimicrobial properties of several silver-containing products used as a coating in pipelines were determined on a pilot scale in order to evaluate the ability of silver to inhibit biofilm formation. The results showed that the coating with silver zeolite at a concentration of 2000 mg L-1 inhibited the formation of a microbial biofilm and prevented the biodeterioration process. Therefore, from our study, it can be concluded that silver zeolite shows greater protection capacity than other silver preparations and presents advantages in relation to other silver coatings that are currently availableCETAQUA Foundatio

Decoherence in an accelerated universe

In this paper we study the decoherence processes of the semiclassical branches of an accelerated universe due to their interaction with a scalar field with given mass. We use a third quantization formalism to analyze the decoherence between two branches of a parent universe caused by their interaction with the vaccum fluctuations of the space-time, and with other parent unverses in a multiverse scenario.Comment: 11 pages, 2 figure

Geometrie analysis and scaling properties of calcite e-twins in the Cameros Basin (NW Iberian Chain, Spain)

One dimensional geometric analysis has been carried out in several scan lines from 885 measures of twins in calcite grains to determine grain width (in microns) and twin density (number of twins.mm-1 ) distributions. Grain width and twin density have a good fit to the log-normal frequency distribution. Twinning in calcite implies intracrystaline deformation mechanism with low shear stress. When the process begins low grain width and calcite twins are developed with a probably random distribution what could be supported by a negative exponential distribution tendency. The twinning process continues until a "critical" value of grain width and density which is going to influence in the scaling process, and becoming the distribution to log-normal type. But some data also conform to a power-law (fractal) frequency distribution from determined range or sizes (300 to WOO mm) and density (2 to W twins.mm-1) with some superimposed random (negative-exponential) elements, possible due to the irregularities at grain scale, but also because this systems show multifractal behavior

Spatial variation of aerosol properties over Europe derived from satellite observations and comparison with model calculations

International audienceAerosol optical depths (AOD) and Angström coefficients over Europe were retrieved using data from the ATSR-2 radiometer on board the ESA satellite ERS-2, for August 1997. Taking advantage of the nadir and forward view of the ATSR-2, the dual view algorithm was used over land to eliminate the influence of the surface reflection. Over sea the AOD was retrieved using only the forward observations. Retrieved aerosol optical properties are in good agreement with those from ground-based sunphotometers. The AOD and Angström coefficients together yield information on the column integrated effective aerosol distribution. Observed regional variations of the AOD and Angström coefficient are related to anthropogenic emissions of aerosol precursors such as SO2 and NOx in the major European industrial and urban areas, and their subsequent transformation into the aerosol phase. The influence of anthropogenic aerosols such as ammonium sulphate and ammonium nitrate on the total AOD is estimated using a regional chemistry transport model. Sulphate is estimated to contribute from 15% in very clean areas to 70% in polluted areas, the contribution of nitrate is between 5% and 25% over most of Europe. This paper shows the great importance of nitrate in summer over The Netherlands

Quantum state of the multiverse

A third quantization formalism is applied to a simplified multiverse scenario. A well defined quantum state of the multiverse is obtained which agrees with standard boundary condition proposals. These states are found to be squeezed, and related to accelerating universes: they share similar properties to those obtained previously by Grishchuk and Siderov. We also comment on related works that have criticized the third quantization approach.Comment: 15 pages, 2 figure

Spatial variation of aerosol properties derived from satellite observations

International audienceAerosol optical depths (AOD) and Angström coefficients over Europe were retrieved using data from the ATSR-2 radiometer on board the ESA satellite ERS-2, for August 1997. Taking advantage of the nadir and forward view of the ATSR-2, the dual view algorithm was used over land to eliminate the influence of the surface reflection. Over sea the AOD was retrieved using only the forward observations. Retrieved aerosol optical properties are in good agreement with those from ground-based sunphotometers. The AOD and Angström coefficients together yield information on the column integrated effective aerosol distribution. Observed regional variations of the AOD and Angström coefficient are related to anthropogenic emissions of aerosol precursors such as SO2 and NOx in the major European industrial and urban areas, and their subsequent transformation into the aerosol phase. The influence of anthropogenic aerosols such as ammonium sulphate and ammonium nitrate on the total AOD is estimated using a regional chemistry transport model. Sulphate is estimated to contribute from 15% in very clean areas to 70% in polluted areas, the contribution of nitrate is between 5% and 25% over most of Europe. This paper shows the great importance of nitrate in summer over The Netherlands

SIDM on FIRE: Hydrodynamical Self-Interacting Dark Matter simulations of low-mass dwarf galaxies

We compare a suite of four simulated dwarf galaxies formed in 10$^{10} M_{\odot}$ haloes of collisionless Cold Dark Matter (CDM) with galaxies simulated in the same haloes with an identical galaxy formation model but a non-zero cross-section for dark matter self-interactions. These cosmological zoom-in simulations are part of the Feedback In Realistic Environments (FIRE) project and utilize the FIRE-2 model for hydrodynamics and galaxy formation physics. We find the stellar masses of the galaxies formed in Self-Interacting Dark Matter (SIDM) with $\sigma/m= 1\, cm^2/g$ are very similar to those in CDM (spanning $M_{\star} \approx 10^{5.7 - 7.0} M_{\odot}$) and all runs lie on a similar stellar mass -- size relation. The logarithmic dark matter density slope ($\alpha=d\log \rho / d\log r$) in the central $250-500$ pc remains steeper than $\alpha= -0.8$ for the CDM-Hydro simulations with stellar mass $M_{\star} \sim 10^{6.6} M_{\odot}$ and core-like in the most massive galaxy. In contrast, every SIDM hydrodynamic simulation yields a flatter profile, with $\alpha >-0.4$. Moreover, the central density profiles predicted in SIDM runs without baryons are similar to the SIDM runs that include FIRE-2 baryonic physics. Thus, SIDM appears to be much more robust to the inclusion of (potentially uncertain) baryonic physics than CDM on this mass scale, suggesting SIDM will be easier to falsify than CDM using low-mass galaxies. Our FIRE simulations predict that galaxies less massive than $M_{\star} < 3 \times 10^6 M_{\odot}$ provide potentially ideal targets for discriminating models, with SIDM producing substantial cores in such tiny galaxies and CDM producing cusps.Comment: 10 Pages, 7 figures, submitted to MNRA

Blended transdiagnostic group CBT for emotional disorders: A feasibility trial protocol

Introduction: Emotional disorders (anxiety and depressive disorders) are a relevant public health concern associated with high prevalence, high costs, and important disability. Therefore, research priorities include designing and testing cost-effective interventions to reach everyone in need. Internet-delivered interventions for emotional disorders are effective and can help to disseminate and implement evidence-based treatments. However, although these treatments are generally effective, not all patients benefit from this treatment format equally. Blended treatments are a new form of intervention that combines the strengths of face-to-face and Internet approaches. Nevertheless, research on blended interventions has focused primarily on individual therapy, and less attention has been paid to the potential of using this format in group psychotherapy. This study aims to analyze the feasibility of blended transdiagnostic group CBT for emotional disorders. The current article describes the study protocol for this trial. Method and analysis: A one-armed pilot trial will be conducted. Participants will be 30 adults suffering from DSM-5 anxiety and/or depressive disorders. The treatment consists of a blended transdiagnostic group intervention delivered during a period of 24 weeks. Groups of 6 to 10 patients will attend a total of eight 2-hour, face-to-face sessions, alternated with the use of an online platform where they will find the contents of the treatment protocol. The intervention has four core components: present-focused awareness, cognitive flexibility, identification and modification of behavioral and cognitive patterns of emotional avoidance, and interoceptive and situational exposure. These components are delivered in 16 modules. Assessments will be performed at baseline, during the treatment, at post-treatment, and at 3-month follow-up. Clinical and treatment acceptability outcomes will be included. Quantitative and qualitative data (participants’ views about blended group psychotherapy) will be analyzed. Ethics and dissemination: The trial has received ethical approval from the Ethics Committee of Universitat Jaume I (September 2019) and will be conducted in accordance with the study protocol, the Declaration of Helsinki, and good clinical practice. The results of this study will be disseminated by presentation at conferences and will be submitted for publication in a peer-reviewed journal. Trial registration: ClinicalTrials.gov Identifier: NCT04008576. Registered 05 July 2019, https://clinicaltrials.gov/ct2/show/NCT0400857