Increased growth rate of anodic porous alumina by use of ionic liquid as electrolyte additive

The use of ionic liquids 1-Butyl-3-methylimidazolium 2-(2-methoxyethoxy) ethyl sulfate and 1-Butyl-3-methylimidazolium tetrafluoborate has been tested in the fabrication of anodic porous alumina. The anodizations of the aluminium substrate have been carried out in oxalic acid in galvanostatic mode. During anodization with 1-Butyl-3-methylimidazolium tetrafluoborate added electrolyte, proper tuning of the current density and of the additive concentration resulted in a three-fold increase of the growth rate as compared to the bare acidic solution with the same acid concentration. This did not cause cracks in the film during growth, and did not affect the regular structure of the pores at the interface with the substrate

Violencia —de Estado y revolucionaria— en la Argentina: Transmisión transgeneracional del trauma migratorio. Consecuencias en la clínica

Gran parte de las migraciones se producen a partir de situaciones traumáticas en que la sociedad se vuelve expulsiva por motivos religiosos, económicos y/o políticos y la tierra madre se torna hostil. Este es el caso de la mayor parte de las familias que emigraron desde Europa a la Argentina a fines del siglo XIX y principios del XX. Esta primera generación traumatizada, hizo en general grandes esfuerzos para adaptarse, siendo la segunda generación la que habría de constituir la mayor parte de la clase media argentina. La tercera generación protagonizó los hechos de los años ’70 con sus características de violencia, incluso armada (violencia de Estado y violencia revolucionaria). Establecemos una relación entre la violencia que sufrieron las familias migrantes, tanto en el país de origen como en el de adopción, y los fenómenos de violencia en la Argentina en los tiempos de la tercera generación, además de la aparición de patologías mentales y orgánicas. Nos preguntamos si la generación de los ’70 llevó a un plano de lucha las fantasías de los antepasados inmigrantes, sometidos y denigrados, donde se evidencia lo relegado y la potencia que adquiere al pasar de la fantasía al acto. Planteamos también los obstáculos que surgen en el nivel contratransferencial en el curso de una terapia familiar psicoanalítica, cuando no sólo los pacientes, sino también los analistas pertenecemos a familias de inmigrantes y hemos estado inmersos en el clima de violencia social de los ’70 y sus consecuencias.Presentamos el material clínico de una familia, siguiendo tramos de la historia de varias generaciones, desde la masacre de la guerra civil española hasta la violencia política en Argentina

Clinical setting influences physiological responses in dental implant patients

Abstract Anxiety is often observed in dental procedures and may cause promote alteration in the physiological responses during implant surgeries. Aim: To evaluate changes in blood pressure and heart rate in patients undergoing dental implant procedures, considering the dental setting as the main variable. Methods: Fifty-five patients who underwent dental implant surgery were evaluated. Thirty-seven were treated at a university clinic and 18 were treated at a private office. Blood pressure and heart rate were measured at the following time-points: at the appointment prior to surgery (T0), immediately before the surgical procedure (T1), during anesthesia (T2), during implant installation (T3), immediately after the surgical procedure (T4) and at the first follow-up appointment after surgery (T5). The data were analyzed by two-way analysis of variance and Student's t-test. Results: The university clinic patients had an increase in heart rate at T5 (t 53 =2.62, p<0.05) compared with private office patients. Systolic blood pressure in university clinic patients was higher at T2 (t 53 =2.86, p<0.01), T3 (t 53 =2.64, p<0.05), and T4 (t 53 =3.15, p<0.01). Diastolic blood pressure at T2 (t 53 =3.15, p<0.01) and T3 (t 53 =3.86, p<0.01) were also higher in university clinic patients. Conclusions: These results suggest that the dental setting is a relevant factor when planning dental implant surgery

The relationship between dental anxiety in children, adolescents and their parents at dental environment

Purpose: The aim of the present study was to compare trait anxiety and dental anxiety among children, adolescents and their parents. Materials and Methods: A cross-sectional study was conducted involving 100 patients from the Pediatric Dentistry Clinic of the Federal University of Parana (Brazil) between the ages of 8 and 17 years (mean age: 10.3; standard deviation: 2.03) and their parents, who responded to Corah′s Dental Anxiety Scale (DAS) and the Trait Anxiety Scale. The data were analyzed using the Mann-Whitney test, analysis of variance and both Pearson′s and Spearman′s correlation coefficients. Results: Ninety percent of children and adolescents and 76% of the parents had moderate anxiety based on the DAS score. Seventy-four percent of children and adolescents and 72% of the parents had moderate anxiety based on the Trait Anxiety Scale score. The trait anxiety and dental anxiety scores were correlated among the adults (rs = 0.64) and children (r = 0.52), whereas no correlation between scores was found among the adolescents. Associations were also found between children′s trait anxiety and the dental and trait anxiety of their parents (both r = 0.43). Conclusions: A moderate degree of dental anxiety was prevalent among the children, adolescents and parents who took part in this investigation, with correlations demonstrated between some trait anxiety and dental anxiety scores

Instabilidade de microssatélite - MSI nos marcadores (BAT26, BAT25, D2s123, D5S346, D17S250) no câncer de reto Microsatellite instability - MSI markers (BAT26, BAT25, D2S123, D5S346, D17S250) in rectal cancer

RACIONAL: O câncer colorretal tem importante componente genético. Os microssatélites são considerados marcadores fenotípicos de prognóstico, resposta terapêutica e de identificação de pacientes com mutação nos genes de reparo do DNA. OBJETIVOS: Avaliar o perfil molecular dos tumores submetidos à microcirurgia endoscópica transanal (TEM) para tratamento do câncer de reto. MÉTODO: Foram selecionados 38 espécimes avaliados segundo o estadiamento patológico. Foram escolhidas amostras da região tumoral e realizada dissecação e extração do DNA. Os tumores colorretais foram testados para instabilidade de microssatélite - MSI utilizando um painel composto de cinco marcadores (BAT25, BAT26, D2S123, D5S346 e D17S2720), técnica da reação em cadeia da polimerase (PCR). RESULTADOS: Nos 38 casos observou-se que 63% eram do sexo masculino e 47% feminino com média de idade de 58,4 anos. Em relação ao tipo tumoral 58% eram adenomas, sendo 24% adenomas de baixo grau e 76% de alto grau; 42% eram carcinomas. Quanto à profundidade de ressecção, verificou-se que 80% dos casos incluíam a gordura perirretal e 20% até a muscular própria. O microssatélite com maior frequência de amplificação foi o BAT26 (100%) e o menor D17S2720 (85,4%). Dezesseis casos (42%) apresentaram MSI; eram dez carcinomas, dois adenomas de baixo grau e quatro de alto grau. Vinte e dois casos (68%) tinham microssatélite estáveis - MSS. A perda alélica dos marcadores de microssatélites foi estatisticamente significante nos casos de carcinoma em relação a adenomas. O microssatélite com maior frequência de amplificação foi o BAT26 (100%) e o menor D17S2720 (85,4%); 16 casos (42%) apresentaram instabilidade de microssatélite - MSI. Desses, dez eram carcinomas, dois adenomas de baixo grau e quatro de alto grau; 22 casos (58%) apresentaram microssatélite estáveis - MSS. CONCLUSÃO: A instabilidade de microssatélite (MSI-H) foi significantemente associada com carcinomas retais, confirmando sua utilização como marcador prognóstico na carcinogênese retal.BACKGROUND: Colorectal cancer has an important genetic component. Microsatellites are considered phenotypic markers of prognosis, therapeutic response and identify patients with mutations in DNA repair genes. AIM: To evaluate the molecular profile of tumors underwent to transanal endoscopic microsurgery - TEM in surgical treatment of rectal cancer. METHOD: Thirty eight surgical specimens were evaluated according to pathological staging and the region of the tumor were dissected and submitted to DNA extraction. The colorectal tumors were tested for microsatellite instability - MSI using a panel of five markers (BAT25, BAT26, D2S123, D5S346, and D17S2720) technique of Polymerase Chain Reaction (PCR). RESULT: From total 63% were male and 47% female, with mean age of 58.4 years. In relation to tumor type adenomas were 58%, 24% low-grade adenomas and 76% high grade; 42% were carcinomas. The depth of resection 80% included the rectal perirenal fat and 20% the muscularis propria. The most frequent microsatellite amplification was BAT26 (100%) and lowest D17S2720 (85.4%). Sixteen patients (42%) were MSI, ten were carcinomas, two low grade adenomas and four high grade. Twenty-two cases (68%) showed microsatellite stable - MSS. The allelic loss of microsatellite markers was statistically significant in cases of carcinoma in relation to adenomas. The most frequent microsatellite amplification was BAT26 (100%) and lower D17S2720 (85.4%), 16 patients (42%) had microsatellite instability - MSI thereof ten were carcinomas, two low grade adenomas, four high-grade adenomas and 22 cases (58%) were microsatellite stable - MSS. CONCLUSION: Microsatellite instability (MSI-H) was significantly associated with rectal carcinomas, confirming its use as a prognostic marker in colorectal carcinogenesis

Clinical setting influences physiological responses in dental implant patients

is often observed in dental procedures and may cause promote alteration in the physiological responses during implant surgeries. Aim: To evaluate changes in blood pressure and heart rate in patients undergoing dental implant procedures, considering the dental setting as the main variable. Methods: Fifty-five patients who underwent dental implant surgery were evaluated. Thirty-seven were treated at a university clinic and 18 were treated at a private office. Blood pressure and heart rate were measured at the following time-points: at the appointment prior to surgery (T0), immediately before the surgical procedure (T1), during anesthesia (T2), during implant installation (T3), immediately after the surgical procedure (T4) and at the first follow-up appointment after surgery (T5). The data were analyzed by two-way analysis of variance and Students t-test. Results: The university clinic patients had an increase in heart rate at T5 (t53=2.62, p<0.05) compared with private office patients. Systolic blood pressure in university clinic patients was higher at T2 (t53=2.86, p<0.01), T3 (t53=2.64, p<0.05), and T4 (t53=3.15, p<0.01). Diastolic blood pressure at T2 (t53=3.15, p<0.01) and T3 (t53=3.86, p<0.01) were also higher in university clinic patients. Conclusions: These results suggest that the dental setting is a relevant fator when planning dental implant surgery

Thermo-Plasmonic Killing of <i>Escherichia coli</i> TG1 Bacteria

Plasmonic photo-thermal therapy (PPTT) is a minimally invasive, drug-free, therapy based on the properties of noble metal nanoparticles, able to convert a bio-transparent electromagnetic radiation into heat. PPTT has been used against cancer and other diseases. Herein, we demonstrate an antimicrobial methodology based on the properties of gold nanorods (GNRs). Under a resonant laser irradiation GNRs become highly efficient light to heat nano-converters extremely useful for PPTT applications. The concept here is to assess the antimicrobial effect of easy to synthesize, suitably purified, water-dispersible GNRs on Escherichia coli bacteria. A control on the GNRs concentration used for the process has been demonstrated critical in order to rule out cytotoxic effects on the cells, and still to be able to generate, under a near infrared illumination, an adequate amount of heat suited to increase the temperature up to &#8776;50 &#176;C in about 5 min. Viability experiments evidenced that the proposed system accomplished a killing efficiency suitable to reducing the Escherichia coli population of about 2 log CFU (colony-forming unit)

Microcirurgia endoscópica transanal e tratamento adjuvante no câncer retal precoce

RACIONAL: A excisão total do mesorreto é considerada a operação padrão no tratamento dos tumores do reto, apesar de não existir comprovação científica de que ela deva ser usada para todos os estádios da doença. Tem sido demonstrado que em casos escolhidos de tumores retais, resultados promissores podem ser conseguidos com tratamento local por microcirurgia endoscópica transanal. Tais tumores, denominados de câncer retal precoce, são tumores T1 - menores do que 4 cm -, bem diferenciados sem invasão angiolinfática pT1 Sm1. Como o risco de comprometimento linfonodal nesses tumores é de aproximadamente 3%, a ressecção local teria grande chance de ser curativa. OBJETIVO: Apresentar os resultados de uma série prospectiva não randômica de pacientes portadores de câncer retal precoce submetidos ao tratamento local por microcirurgia endoscópica transanal. MÉTODOS: Entre 2002 e 2010, 38 pacientes avaliados por protocolo pré-operatório como portadores câncer retal precoce foram submetidos à ressecção local endoscópica microcirúrgica de toda a parede retal com o tumor quando localizado entre 2 e 8 cm da linha pectínea. A avaliação pré-operatória consistiu de toque retal, retossigmoidoscopia rígida para macrobiópsias, enema opaco e/ou colonoscopia, ultrassonografia endoretal e abdominal, tomografia axial computadorizada do abdome, radiografia do tórax e dosagem sérica do CEA. Realizou-se seguimento pós-operatório endoscópico e ultrassonográfico endoretal a cada três meses nos dois primeiros anos, e a cada seis nos próximos três anos, além de dosagem do CEA a cada seis meses nesse mesmo período de cinco anos. Avaliou-se a recidiva tumoral, morbidade e mortalidade. RESULTADOS: Após avaliação anatomopatológica da lesão, 29 cânceres retais precoces foram categorizados como de baixo risco e nove sendo de alto. O seguimento na série variou de um a sete anos. Recidiva tumoral foi confirmada em dois casos dos 38 (5,26%), uma lesão considerada de alto e a outra de baixo risco. CONCLUSÃO: Microcirurgia endoscópica transanal, associada ou não à quimioradioterapia, pode ser considerada atualmente o padrão-ouro na ressecção retal local, apresentando resultados animadores em casos escolhidos de tumores retais precoces de baixo risco