1946 Fertilizer Recommendations for Field Crops, Permanent Pastures and Hay Fields

PDF pages:

Patients' Perceptions and Treatment Effectiveness

An extensive literature relating patients’ expectations to treatment outcomes has not addressed the determinants of these expectations. We argue that treatment history is part of a reference point that influences patients’ expectations of how effective further treatment might be, thus influencing whether to proceed with additional treatment or not. We hypothesize that those patients with unsuccessful prior treatments have diminished expected improvement from subsequent treatments. Prospect theory provides a theoretical foundation for reference frame effects, and the model is tested with data on patients diagnosed with idiopathic intracranial hypertension. Our results support the reference frame hypothesis.Prospect Theory, Treatment Outcomes, Treatment History, Misclassification, Monotone Rank Estimator

Mammalian Clusterin associated protein 1 is an evolutionarily conserved protein required for ciliogenesis

BACKGROUND: Clusterin associated protein 1 (CLUAP1) was initially characterized as a protein that interacts with clusterin, and whose gene is frequently upregulated in colon cancer. Although the consequences of these observations remain unclear, research of CLUAP1 homologs in C. elegans and zebrafish indicates that it is needed for cilia assembly and maintenance in these models. To begin evaluating whether Cluap1 has an evolutionarily conserved role in cilia in mammalian systems and to explore the association of Cluap1 with disease pathogenesis and developmental abnormalities, we generated Cluap1 mutant mice. METHODS: Cluap1 mutant embryos were generated and examined for gross morphological and anatomical defects using light microscopy. Reverse transcription PCR, β-galactosidase staining assays, and immunofluorescence analysis were used to determine the expression of the gene and localization of the protein in vivo and in cultured cell lines. We also used immunofluorescence analysis and qRT-PCR to examine defects in the Sonic hedgehog signaling pathway in mutant embryos. RESULTS: Cluap1 mutant embryos die in mid-gestation, indicating that it is necessary for proper development. Mutant phenotypes include a failure of embryonic turning, an enlarged pericardial sac, and defects in neural tube development. Consistent with the diverse phenotypes, Cluap1 is widely expressed. Furthermore, the Cluap1 protein localizes to primary cilia, and mutant embryos were found to lack cilia at embryonic day 9.5. The phenotypes observed in Cluap1 mutant mice are indicative of defects in Sonic hedgehog signaling. This was confirmed by analyzing hedgehog signaling activity in Cluap1 mutants, which revealed that the pathway is repressed. CONCLUSIONS: These data indicate that the function of Cluap1 is evolutionarily conserved with regard to ciliogenesis. Further, the results implicate mammalian Cluap1 as a key regulator of hedgehog signaling and as an intraflagellar transport B complex protein. Future studies on mammalian Cluap1 utilizing this mouse model may provide insights into the role for Cluap1 in intraflagellar transport and the association with colon cancer and cystic kidney disorders

Coiled-coil domain containing 42 (Ccdc42) is necessary for proper sperm development and male fertility in the mouse

Spermiogenesis is the differentiation of spermatids into motile sperm consisting of a head and a tail. The head harbors a condensed elongated nucleus partially covered by the acrosome-acroplaxome complex. Defects in the acrosome-acroplaxome complex are associated with abnormalities in sperm head shaping. The head-tail coupling apparatus (HTCA), a complex structure consisting of two cylindrical microtubule-based centrioles and associated components, connects the tail or flagellum to the sperm head. Defects in the development of the HTCA cause sperm decapitation and disrupt sperm motility, two major contributors to male infertility. Here, we provide data indicating that mutations in the gene Coiled-coil domain containing 42 (Ccdc42) is associated with malformation of the mouse sperm flagella. In contrast to many other flagella and motile cilia genes, Ccdc42 expression is only observed in the brain and developing sperm. Male mice homozygous for a loss-of-function Ccdc42 allele (Ccdc42(KO)) display defects in the number and location of the HTCA, lack flagellated sperm, and are sterile. The testes enriched expression of Ccdc42 and lack of other phenotypes in mutant mice make it an ideal candidate for screening cases of azoospermia in humans

Mennonite missionaries and African Independent Churches: the development of an Anabaptist missiology in West Africa: 1958-1967

This dissertation analyzes Mennonite missionary engagement with African Independent Churches in West Africa. The engagement between missionaries and indigenous churches gave rise to a novel mission interaction with a non-western form of Christianity. It led to the early development of mission strategy and theory from an intentionally Anabaptist perspective. Based upon close analysis of archival material, the dissertation examines the extended encounter between missionaries and Independents in southeastern Nigeria between 1958 and 1967. It places the encounter within the context of the religious history of both groups and outlines the influence of the experience on subsequent mission work. This case study sheds new light on the emergence of African indigenous Christian movements and western Christians’ interaction with those movements during the period of decolonization and African nationalism. The history that this study constructs shows that the religious and missiological assumptions that each party brought to the encounter complicated their relationship. The Independents’ religious history led them to expect missionaries to establish traditional mission educational and healthcare institutions that would reinforce their well-being. Missionaries Edwin and Irene Weaver and their colleagues were hesitant to do so, since their experience in India had convinced them that such institutions caused dependency on foreign funds and impeded indigenization. They focused, rather, on encouraging better relationships between estranged Independents and mission churches, capacitating Independent churches through biblical training, and reinforcing Independents’ indigenous identity. Yet some Nigerian Independents insisted on a traditional mission relationship and its accompanying Mennonite identity. Missionaries borrowed mission theory about indigenization from the wider missionary movement, but applied and modified it over time, finally incorporating it into an Anabaptist missionary approach for work in Nigeria, Ghana, Cote d’Ivoire and the Republic of Benin. This study suggests that while relationships between streams of the Christian movement are conditioned by their different religious histories and cultures, they nevertheless generate missiological insights. Through this engagement missionaries articulated an Anabaptist missiology that became influential throughout Africa. In turn, the Mennonite missionary presence enabled some Nigerian Independents to network successfully with the world Christian movement via their Mennonite affiliation

Locally managed irrigation systems: essential tasks and implications for assistance, management transfer and turnover programs

This monograph examines the construction, operation and maintenance tasks that shape the nature of locally managed irrigation systems. The objective of the book is to identify relevant experiences and lessons for staff who are responsible for working with locally managed systems in three types of programs: direct assistance to existing locally managed irrigation systems, turnover of public owned systems to local management, and transfer of partial management to farmer groups within larger systems that remain publicly controlled.Irrigation management, Irrigation systems, Irrigation scheduling, Privatization, Farmer participation, Water management, Water rights, Water allocation, Resource management, Organizational dynamics, Conflict, Communication, Training, Farm Management, Institutional and Behavioral Economics,

Report of Feasibility Study Task Force for Marietta Truck Growers Association

Exact date of working paper unknown

An inducible CiliaGFP mouse model for in vivo visualization and analysis of cilia in live tissue

BACKGROUND: Cilia are found on nearly every cell type in the mammalian body, and have been historically classified as either motile or immotile. Motile cilia are important for fluid and cellular movement; however, the roles of non-motile or primary cilia in most tissues remain unknown. Several genetic syndromes, called the ciliopathies, are associated with defects in cilia structure or function and have a wide range of clinical presentations. Much of what we know about the formation and maintenance of cilia comes from model systems like C. elegans and Chalmydomonas. Studies of mammalian cilia in live tissues have been hampered by difficulty visualizing them. RESULTS: To facilitate analyses of mammalian cilia function we generated an inducible Cilia(GFP) mouse by targeting mouse cDNA encoding a cilia-localized protein somatostatin receptor 3 fused to GFP (Sstr3::GFP) into the ROSA26 locus. In this system, Sstr3::GFP is expressed from the ubiquitous ROSA26 promoter after Cre mediated deletion of an upstream Neo cassette flanked by lox P sites. Fluorescent cilia labeling was observed in a variety of live tissues and after fixation. Both cell-type specific and temporally regulated cilia labeling were obtained using multiple Cre lines. The analysis of renal cilia in anesthetized live mice demonstrates that cilia commonly lay nearly parallel to the apical surface of the tubule. In contrast, in more deeply anesthetized mice the cilia display a synchronized, repetitive oscillation that ceases upon death, suggesting a relationship to heart beat, blood pressure or glomerular filtration. CONCLUSIONS: The ability to visualize cilia in live samples within the Cilia(GFP) mouse will greatly aid studies of ciliary function. This mouse will be useful for in vivo genetic and pharmacological screens to assess pathways regulating cilia motility, signaling, assembly, trafficking, resorption and length control and to study cilia regulated physiology in relation to ciliopathy phenotypes

Diabetes reduces bone marrow and circulating porcine endothelial progenitor cells, an effect ameliorated by atorvastatin and independent of cholesterol

Bone marrow derived endothelial progenitor cells (EPCs) are early precursors of mature endothelial cells which replenish aging and damaged endothelial cells. The authors studied a diabetic swine model to determine if induction of DM adversely affects either bone marrow or circulating EPCs and whether a HMG-CoA reductase inhibitor (statin) improves development and recruitment of EPCs in the absence of cholesterol lowering. Streptozotocin was administered to Yorkshire pigs to induce DM. One month after induction, diabetic pigs were treated with atorvastatin (statin, n = 10), ezetimibe (n = 10) or untreated (n = 10) and evaluated for number of bone marrow and circulating EPCs and femoral artery endothelial function. There was no effect of either medication on cholesterol level. One month after induction of DM prior to administration of drugs, the number of bone marrow and circulating EPCs significantly decreased (P < 0.0001) compared to baseline. Three months after DM induction, the mean proportion of circulating EPCs significantly increased in the atorvastatin group, but not in the control or ezetimibe groups. The control group showed progressive reduction in percentage of flow mediated vasodilatation (no dilatation at 3 months) whereas the atorvastatin group and ezetimibe exhibited vasodilatation, 6% and 4% respectively. DM results in significant impairment of bone marrow and circulating EPCs as well as endothelial function. The effect is ameliorated, in part, by atorvastatin independent of its cholesterol lowering effect. These data suggest a model wherein accelerated atherosclerosis seen with DM may, in part, result from reduction in EPCs which may be ameliorated by treatment with a statin