Chap is upregulated in mouse models of pathological cardiac hypertrophy.

<p>(A) Quantitative RT-PCR showing increased mRNA levels of both ChapA and B in hearts of transgenic mice expressing constitutively active calcineurin A (CnA Tg) (WT: n = 5, CnA Tg: n = 6; Students <i>t-test</i>: CnA Tg vs WT). (B) Western blot for CHAPa and CHAPb in adult wildtype hearts CnA Tg hearts (WT: n = 5, CnA Tg: n = 6). In wildtype hearts, CHAPa is the dominant isoform while in CnA Tg hearts both CHAPa and b were significantly upregulated. (C) Quantitative RT-PCR for ChapA showing mice subjected to transverse aortic constriction (TAC) for 1 day, 1 week and 3 weeks (n = 4, n = 4, and n = 10 respectively), with no significant regulation of ChapA mRNA compared to sham controls (n = 3, n = 3 and n = 7 respectively). (D) Quantitative RT-PCR for ChapB showing mice subjected to TAC with significant upregulation of ChapB mRNA (number of mice are same as in C). Gapdh was used as internal control in all experiments. <i>T-test</i> with Welch’s correction TAC vs sham: *, p<0.05; **, p<0.01;***, p<0.001.</p

CHAPb Tg mice show decrease cardiac performance.

<p><b>(</b>A) Representative 4-chamber view MRI images of wt and CHAPb Tg heart. Enlarged LA in CHAPb Tg is indicated with *. (B-G) MRI measurements of the left ventricle of wt (n = 4, white bars) and CHAPb Tg (n = 5, black bars) animals at 6 months of age. ED volume (B), ES volume (C), ejection fraction (D), cardiac output (E), LV mass ED (F) and LV mass ES (G). (H) Experimental setup for single membrane-permeabilized cardiomyocyte measurements to determine sarcomere function. Sarcomere force measurements at one month of age showed no difference in passive force (I; Fpas), while maximum force (J; Fmax) and Ca²⁺-sensitivity (K; pCa₅₀) were reduced in CHAPb Tg (n = 11, black bars) compared to wt (n = 12, white bars). ED (end diastolic), ES (end systolic) and LV (left ventricular). <i>t</i>-test: *, p<0.05; **, p<0.01; ***, p<0.001.</p

Hypertrophy and left atrial enlargement in CHAP Tg hearts.

<p>(A-C) Wt (left panels) and CHAPb Tg (right panels) at 3 and 6 months of age. (A) HE stained overview section. The left atrium in CHAPb Tg hearts is enlarged (indicated by *) compared to wt litter mates. (B) Higher magnification of left ventricle. In the left ventricle of CHAPb Tg hearts the cardiomyocytes are hypertrophic. (C) Sirius red staining of the left ventricle showing increase in interstitial fibrosis in CHAPb Tg. (D) Myocardial volume of the left atrium (left panels) and right atrium (right panels) in wt (white bars) and CHAPb Tg (black bars) hearts at 3 months of age (wt n = 4, Tg n = 4, T-test p< 0.001). (E) CHAPb Tg heart with severe phenotype showing pronounced atrial enlargement, filled by a thrombus and thickening of the ventricles. Scale bars 1 mm in A and D, 50 μm in B, C.</p

Sarcomeric organization is disturbed and actin signalling is increased in CHAPb Tg hearts.

<p>(A) Immunostaining for CHAP (green) and α-actinin (red) show stress fiber formation (white arrows) in CHAP Tg hearts (B) Electron microscopy analysis of wt and CHAPb Tg hearts at 6 months of age. In CHAPb Tg the sarcomeres were irregular and Z-discs and intercalated discs (black arrow heads) are disorganized, while M-bands are absent (white arrow heads) (C) Wt and CHAPb Tg hearts at 6 months of age stained for RhoA (red). In wt mice RhoA is localized at the membrane of cardiomyocytes and shows a sarcomeric expression pattern. In CHAPb Tg hearts sarcomeric expression of RhoA is absent and membrane expression is increased. (D) Western blot analysis of 2 wt and 3 CHAPb Tg hearts at 6 months of age for RhoA (24kDa), α-actinin (100kDa), actin (42kDa), Ezrin(80 kDa)/moesin(80 kDa)/radixin (75 kDa; ERM), cofilin (19kDa), SRF (40 – 67kDa) and MEF2 (40-65kDa). GAPDH (38kDa) is used as loading control. (E) Working model: in adult wt mice CHAPa is localized at the Z-disc, leading to abundance of monomeric G-actin and subsequent low expression of SRF target genes. In CHAPb Tg mice CHAPb expression results in activation of RhoA, leading to a shift from G-actin to F-actin, binding of co-factors to SRF and activation of SRF target genes, such as ANF, BNP and β-MHC. Scale bars 20 μm in A and C, 1 μm in B.</p

Decreased Connexin 40 expression in CHAPb Tg left atrium at 6 months of age leads to atrio-ventricular conduction delay.

<p>(A) Immunohistochemical staining showing Connexin 40 expression in wt (left panels) and CHAPb Tg (right panels) left (upper panels) and right (lower panels) atria. (B,C) qPCR analysis of Connexin 40 expression in the left (B) and right (C) atrium (wt n = 3, Tg n = 3 T-test, **,p<0.01). (D,E) ECG analysis of wt (n = 3) and CHAPb Tg (n = 8) mice showing P duration (N.S.) (D) and PR interval (p = 0.02, T-test) (E). Scale bars 50 μm.</p

Expression of hypertrophy markers and collagens in left ventricle.

<p>qPCR analysis showing mRNA expression of <i>Nppa</i> (A), <i>Nppb</i> (B), <i>Myh7</i> (C), <i>CollagenI</i> (D) <i>CollagenIII</i> (E), <i>Serca2</i> (F), <i>ChapA</i> (G), <i>ChapB</i> (H) and endogenous <i>ChapB</i> (I) in the left ventricle of wt (white bars) and CHAPb Tg (black bars) mice. <i>Gapdh</i>, <i>Pgk</i> and <i>H2a</i> were used as internal controls. (wt n = 3, Tg n = 3, T-test: *, p<0.05; **,p<0.01;***, p<0.001).</p

Collision detection on transmission lines with optical interferometer

V diplomski nalogi skušamo ugotoviti, v kolikšni meri je možno zaznavati in klasificirati trke na jeklenicah daljnovodov z optičnim interferometrom. Na začetku predstavimo osnovne pojme interferometrije in opišemo uporabljen optični interferometer. V jedru diplomske naloge natančneje opišemo eksperimentalni protokol in obdelavo signalov. Nadaljujemo z implementacijo algoritmov za segmentacijo in klasifikacijo zajetih signalov ter predstavimo dobljene rezultate. Segmentacijo izvedemo v domeni števila prehodov signala skozi ničlo, za klasifikacijo pa uporabimo večplastno nevronsko mrežo z algoritmom vzvratnega učenja. Rezultati študije nakazujejo, da sta implementirani segmentacija in klasifikacija uspešni v 77 % izvedenih trkov različnih predmetov.We analyse feasibility of collision detection on transmission lines with optical interferometer. We first provide a brief introduction into interferometry, along with a description of the optical interferometer used for measurements in this study. Afterwards, we describe the conducted experimental protocol and signal processing methodology. The focus is on implementation of algorithms for signal segmentation and collision classification. We used zero-crossing algorithm to transform signals into segmentation domain. Classification of collisions is done with a multilayer neural network trained by the backpropagation algorithm. The results demonstrate an average success rate of 77% for segmentation and classification of collision with five different objects

Sistematización de la experiencia de un ambiente de aprendizaje enriquecido por TIC durante la práctica clínica en fisioterapia cardiopulmonar en un hospital de nivel II de la ciudad de Cali

Esta investigación se centra en la caracterización de la experiencia de 4 estudiantes de fisioterapia de IX semestre de la Institución Universitaria Escuela Nacional del Deporte (IUEND) durante la implementación de un ambiente de aprendizaje enriquecido con Tecnologías de la Información y la Comunicación (TIC) en la práctica clínico – asistencial en Salud Cardiopulmonar; la cual se fundamenta en el hacer y pone a prueba las bases conceptuales del ciclo de fundamentación; todo esto con el fin de identificar las experiencias significativas que facilitan el aprendizaje y desarrollo de competencias clínicas, además analizar si este tipo de estrategias de enseñanza -aprendizaje permite al estudiante y al docente asesor superar inconvenientes propios de la práctica clínica como: optimizar tiempos de atención a pacientes, estudio independiente y trabajo colaborativo, retomar e integrar gran cantidad de conceptos y procedimientos aprendidos en IV semestre con las nuevas experiencias y la realidad del paciente; y a la vez cumplir con funciones administrativas propios del rol del fisioterapeuta asistencial (estadística, indicadores, desarrollo de guías, etc.) que dificultan el proceso de aprendizaje; concluyendo que los ambientes mediados por TIC pueden lograr superar estas dificultades y favorecer finalmente el aprendizaje significativo (juicio clínico), en el que se fundamenta el ciclo de práctica profesional

assay information of the mouse qualitycontrol samples for HeliScopeCAGE

a tab delimited flat file (SDRF file) describing the experimental details for mouse quality control samples for the standard protocol of the HeliScopeCAGE protoco

assay information of the mouse tissue samples for HeliScopeCAGE

a tab delimited flat file (SDRF file) describing the experimental details for mouse tissue samples for the standard protocol of the HeliScopeCAGE protoco