Separation of river network–scale nitrogen removal among the main channel and two transient storage compartments

Transient storage (TS) zones are important areas of dissolved inorganic nitrogen (DIN) processing in rivers. We assessed sensitivities regarding the relative impact that the main channel (MC), surface TS (STS), and hyporheic TS (HTS) have on network denitrification using a model applied to the Ipswich River in Massachusetts, United States. STS and HTS connectivity and size were parameterized using the results of in situ solute tracer studies in first‐ through fifth‐order reaches. DIN removal was simulated in all compartments for every river grid cell using reactivity derived from Lotic Intersite Nitrogen Experiment (LINX2) studies, hydraulic characteristics, and simulated discharge. Model results suggest that although MC‐to‐STS connectivity is greater than MC‐to‐HTS connectivity at the reach scale, at basin scales, there is a high probability of water entering the HTS at some point along its flow path through the river network. Assuming our best empirical estimates of hydraulic parameters and reactivity, the MC, HTS, and STS removed approximately 38%, 21%, and 14% of total DIN inputs during a typical base flow period, respectively. There is considerable uncertainty in many of the parameters, particularly the estimates of reaction rates in the different compartments. Using sensitivity analyses, we found that the size of TS is more important for DIN removal processes than its connectivity with the MC when reactivity is low to moderate, whereas TS connectivity is more important when reaction rates are rapid. Our work suggests a network perspective is needed to understand how connectivity, residence times, and reactivity interact to influence DIN processing in hierarchical river systems

Nitrogen fixation by the savanna tree Philenoptera violacea (Klotzsch) Schrire (Apple leaf) of different ages in a semi-arid riparian landscape

AbstractThe acquisition of nitrogen for growth and maintenance is essential for plants, and having multiple strategies for that acquisition is especially important for those colonizing nutrient poor substrates. Philenoptera violacea (Apple leaf) is a prominent tree in nutrient poor savanna and alluvial soils near rivers in southern Africa, where nutrient availabilities are highly variable in space and time. We investigated nitrogen fixation in P. violacea within riparian corridors flanking the Sabie River in Kruger National Park (KNP) in the Lowveld in northeastern South Africa using the natural 15N abundance technique. Results indicated that P. violacea fixes atmospheric nitrogen and this varies with life history stage. We found that foliar δ15N levels were significantly lower in all life stage classes of P. violacea compared with the reference plant D. mespiliformis growing in open riparian forest. In addition δ15N values were significantly different within the different life stages of P. violacea with the leaves of saplings and juvenile plants having significantly lower δ15N levels than mature plants. While δ15N values increased with age, foliar nitrogen concentration values declined, with leaves from sapling P. violacea having significantly higher total nitrogen than adults and juveniles, which were in turn significantly higher than juvenile D. mespiliformis. However, foliar δ15N levels in seedlings of P. violacea growing in a high nutrient environment in flood debris piles did not differ from levels recorded in seedlings of the reference tree. This study confirms that P. violacea is able to fix nitrogen, but it is dependant on soil conditions and the life stage of the trees

Geochronology of granitic rocks from the Ruangwa region, southern Tanzania: links with NE Mozambique and beyond

New U–Pb zircon LA-ICP-MS data are presented for 4 granitoid bodies which intrude high grade gneisses of the previously unmapped Ruangwa region in southern Tanzania. The study area forms part of the late Neoproterozoic East African Orogen (EAO). The oldest unit, a coarse-grained migmatitic granitic orthogneiss gave an early Neoproterozoic (Tonian) crystallization age of 899 ± 9/16 Ma, which is similar to, but significantly younger than, Stenian-Tonian basement ages in areas relatively nearby. Crust of this age may extend as far north as the major Phanerozoic Selous Basin, north of which Archaean protolith ages predominate (the “Western Granulites”), except for the juvenile Neoproterozoic “Eastern Granulites”, which are not represented in the study area. To the south, the Tonian crust of the study area provides a tentative link with the Marrupa Complex in NE Mozambique. A granite pluton, dated at 650 ± 5/11 Ma is broadly coeval with the main Pan-African tectono-thermal event in the East African Orogen that is recorded across Tanzania north of the Selous Basin. Zircons in this granite contain inherited cores at ca. 770 Ma. This age is within the range of dates obtained from south and west of the study area from juvenile granitoid orthogneisses which might be related to a widespread, but poorly understood, early phase of Gondwana assembly along an Andean-type margin. South of the study area, in NE Mozambique, the latest orogenic events occurred at ca. 550 Ma, and are sometimes attributed to the Ediacaran-aged “Kuunga Orogeny”. While metamorphic dates of this age have been recorded from the EAO north of the Selous Basin, magmatic rocks of this event have not been recognized in Tanzania. The two youngest granitoids of the present study are thus the first 500–600 Ma igneous rocks reported from the region. A weakly deformed very coarse-grained granite pluton was dated at 591 ± 4/10 Ma, while a very late, cross-cutting, undeformed granite dyke gave an intrusive age of 549 ± 4/9 Ma. The granitoids ages presented in this study contain elements that are characteristic of the northern, Tanzania-Kenya, segment of the East African Orogen and of the southern, Mozambique, segment. The Tonian orthogneiss sample is typical of (but somewhat younger than) the Marrupa Complex of NE Mozambique. No zircon inheritance was recorded in the sample, typical of the juvenile Marrupa Complex. On the other hand, the ca. 650 Ma granite pluton has an age that is typical of the northern segment of the orogen; this is the first recorded granite of that age intruded into the Tonian-dominated crust of southern Tanzania or NE Mozambique. The two younger granites have provided dates that are typical of the southern segment of the orogen, and that of the Kuunga Orogen. The study area thus appears to represent an area of transitional crust straddling two complex and contrasting segments of the East African Orogen, with elements of both segments present and evidence for a ca. 770 Ma event which appears to be quite widespread and may relate to the early phases of Gondwana amalgamation in southern East Africa

A multicenter retrospective study of childhood brucellosis in Chicago, Illinois from 1986 to 2008

SummaryObjectivesTo determine risk factors in children for the acquisition of Brucella, clinical presentation, treatment, and disease outcomes.MethodsA retrospective multicenter chart review was undertaken of children identified with brucellosis from 1986 to 2008 at three tertiary care centers in Chicago, Illinois, USA. The charts were reviewed for data regarding risk factors for acquisition, clinical presentation, and outcomes.ResultsTwenty-one charts were available for review. The median age was 6.5 years (range 2–14 years); 62% were female. Ethnic background was 67% Hispanic and 24% Arabic. Risk factors included travel to an endemic area (86%), particularly Mexico, and consumption of unpasteurized milk products (76%). Common findings included fever (95%), bacteremia (86%), elevated liver transaminases (80%), constitutional symptoms (76%), splenomegaly (60%), and hepatomegaly (55%). Relapse occurred in three of six subjects started on single drug treatment, but in only one of 15 subjects who started on two or more drugs (p=0.053). No relapses occurred in children whose initial therapy included rifampin or those administered three-drug regimens.ConclusionsBrucella is an infrequent pathogen but should be considered in children with compatible epidemiologic and clinical characteristics. Blood cultures should be obtained, and initial therapy with two or more drugs may decrease the risk of relapse

Geochronology and structure of the eastern margin of the Tanzania Craton east of Dodoma

The precise position, nature and U-Pb zircon geochronology of the eastern margin of the Tanzania Craton has been studied in the Mpwapwa area, some 60 km east of Dodoma, central Tanzania, in a number of field transects over a ca. 45 km strike length of the craton margin. The rocks to the east of the Tanzania Craton in this area either belong to the Palaeoproterozoic Usagaran belt, or the “Western Granulite” terrane of the Neoproterozoic East African Orogen, according to different authors. The eastern part of the craton is underlain by typical Neoarchaean migmatitic grey granodioritic orthogneisses dated by ICP-MS at 2674 ± 73 Ma. There is a gradual increase in strain eastwards in these rocks, culminating in a 1 to 2 km wide, locally imbricated, ductile thrust/shear zone with mylonites indicating an oblique top-to-the-NW, transpressional sense of movement. East of the craton-edge shear zone, a series of high-grade supracrustal rocks are termed the “Mpwapwa Group”, in view of uncertain age and regional lithostratigraphic correlations. There is an apparent east-west lithological zonation of Mpwapwa Group parallel to the craton margin shear zone. In the west, immediately adjacent to the craton, the group consists of typical “shelf facies” metasediments (marbles, calc-silicates, quartzites etc.). U-Pb dating of detrital zircons from two Mpwapwa Group quartzite samples from this marginal zone contain only Archaean detritus, constraining their maximum depositional age to > ca. 2.6 Ga and suggesting that the group is Neoarchaean in age. The shelf rocks pass eastwards into garnet and kyanite-bearing semi-pelitic gneisses interlayered with bimodal mafic-felsic gneisses, where the mafic amphibolite gneisses may represent meta-basalts and the felsic rocks may have meta-rhyolite, -granite or –psammite protoliths. Massive garnet-clinopyroxene amphibolite layers in the Mpwapwa Group gneisses may have been intrusive mafic sills and possibly correlate with the Palaeoproterozoic Isimani Suite, which outcrops south of the study area and includes 2 Ga eclogites. Zircons from a quartzo-feldsapthic gneiss sample from the bimodal gneisses were dated and showed it to be a probable Neoarchaean rock which underwent metamorphism during the Palaeoproterozoic Usagaran event at ca. 1950 Ma. This event was broadly coeval with subduction, closure of an ocean basin and eclogite formation further south and led to the initial juxtaposition of the two Archaean blocks. The metamorphism probably dates the tectonic event when the Archaean Mpwapwa Group rocks were juxtaposed against the orthogneissic Tanzania Craton. The Mpwapwa Group was intruded by weakly foliated biotite granite at 1871 ± 35 Ma. Zircons in the granite have metamorphic rims dated between 550 and 650 Ma that grew during the East African orogenic event

Prenatal exposure to recreational drugs affects global motion perception in preschool children

Chakraborty, A. et al. Prenatal exposure to recreational drugs affects global motion perception in preschool children. Sci. Rep. 5, 16921; doi: 10.1038/srep16921 (2015).Prenatal exposure to recreational drugs impairs motor and cognitive development; however it is currently unknown whether visual brain areas are affected. To address this question, we investigated the effect of prenatal drug exposure on global motion perception, a behavioural measure of processing within the dorsal extrastriate visual cortex that is thought to be particularly vulnerable to abnormal neurodevelopment. Global motion perception was measured in one hundred and forty-five 4.5-year-old children who had been exposed to different combinations of methamphetamine, alcohol, nicotine and marijuana prior to birth and 25 unexposed children. Self-reported drug use by the mothers was verified by meconium analysis. We found that global motion perception was impaired by prenatal exposure to alcohol and improved significantly by exposure to marijuana. Exposure to both drugs prenatally had no effect. Other visual functions such as habitual visual acuity and stereoacuity were not affected by drug exposure. Prenatal exposure to methamphetamine did not influence visual function. Our results demonstrate that prenatal drug exposure can influence a behavioural measure of visual development, but that the effects are dependent on the specific drugs used during pregnancy.This research was supported by the National Institutes on Drug Abuse grants 2RO1DA014948 and RO1DA021757 and the Auckland Medical Research Foundation

Atrial Fibrillation in Africa-An Underreported and Unrecognized Risk Factor for Stroke:A Systematic Review

Over three-quarters of deaths from cardiovascular disease and diabetes occur in low- and middle-income countries, which include many African countries. Global studies showed that the prevalence of the cardiac arrhythmia atrial fibrillation (AF) appeared to be lower in Africa. A systematic search of PubMed and African Journals Online was conducted to determine the prevalence of AF and associated stroke risk factors in Africa and to quantify the need for screening. The publications search yielded a total of 840 articles of which 41 were included. AF was often not identified as the disease of primary interest with its own risks. Data on prevalence in the general population was scarce. The prevalence of stroke risk factors showed a large variation between studies, as well as within clustered subpopulations. AF in Africa is under-reported in published reports. The study types and populations are highly heterogeneous, making it difficult to draw a definitive conclusion on AF prevalence

TgDrpC, an atypical dynamin‐related protein in Toxoplasma gondii, is associated with vesicular transport factors and parasite division

Dynamin‐related proteins (Drps) are involved in diverse processes such as organelle division and vesicle trafficking. The intracellular parasite Toxoplasma gondii possesses three distinct Drps. TgDrpC, whose function remains unresolved, is unusual in that it lacks a conserved GTPase Effector Domain, which is typically required for function. Here, we show that TgDrpC localizes to cytoplasmic puncta; however, in dividing parasites, TgDrpC redistributes to the growing edge of the daughter cells. By conditional knockdown, we determined that loss of TgDrpC stalls division and leads to rapid deterioration of multiple organelles and the IMC. We also show that TgDrpC interacts with proteins that exhibit homology to those involved in vesicle transport, including members of the adaptor complex 2. Two of these proteins, a homolog of the adaptor protein 2 (AP‐2) complex subunit alpha‐1 and a homolog of the ezrin–radixin–moesin (ERM) family proteins, localize to puncta and associate with the daughter cells. Consistent with the association with vesicle transport proteins, re‐distribution of TgDrpC to the IMC during division is dependent on post‐Golgi trafficking. Together, these results support that TgDrpC contributes to vesicle trafficking and is critical for stability of parasite organelles and division

Global wave loads on a damaged ship

A computational tool was applied based on a two dimensional linear method to predict the hydrodynamic loads for damaged ships. Experimental tests on a ship model have also been carried out to predict the hydrodynamic loads in various design conditions. The results of the theoretical method and experimental tests are compared to validate the theoretical method. The extreme wave induced loads have been calculated by short term prediction. For the loads in intact condition, the prediction with duration of 20 years at sea state 5 is used, while for loads in damaged conditions the prediction in 96 hours exposure time at sea 3 is used. The maximum values of the most probable extreme amplitudes of dynamic wave induced loads in damaged conditions are much less than those in intact condition because of the reduced time. An opening could change the distribution of not only stillwater bending moment but also wave-induced bending moment. It is observed that although some cross sections are not structurally damaged, the total loads acting on these cross sections after damage may be increased dramatically compared to the original design load in intact condition