Transatlantic Politics After the 2008 Election

The fundamental realities on which the Atlantic relationship rests remain in place and are highly unlikely to disappear at any time in the foreseeable future. Whoever the American voters choose in the 2008 presidential election, Europe is likely to experience a political honeymoon with the new President and feel relieved at the departure of the Bush Administration. However, it is important to avoid the misconception that has all too frequently distorted discussion of this subject during the past eight years: that the largely harmonious relations between the US and its European partners were rudely interrupted by the coming to power of the Bush Administration with what are said to be its unilateral, aggressive and warlike habits. Issues such as Iran, the Middle East, terrorism and international trade will ensure that there will not be the kind of easy harmony and seamless multilateralism that some observers have explicitly or implicitly ascribed to the pre-George W. Bush years and assumed would re-emerge in the post-Bush era. On the other hand, the fundamental realities on which the Atlantic relationship rests remain in place and are highly unlikely to disappear at any time in the foreseeable future

La política exterior en las elecciones presidenciales de EEUU después del “Super Martes”

Tras reducirse el número de candidatos después del “Super Martes”, los observadores podrán centrar más su atención a partir de ahora en lo que una presidencia de McCain, Clinton u Obama supondría en política exterior. Después de casi un año de campaña política, las esperadísimas primarias del “Super Martes” han clarificado finalmente la competición por la elección presidencial. Las elecciones generales propiamente dichas no tendrán lugar hasta el 6 de noviembre, dentro de casi nueve meses, pero ya es seguro que el candidato republicano será el senador John McCain, y el candidato demócrata será o bien Hillary Rodham Clinton o Barak Obama. Al reducirse el número de candidatos, los observadores dentro y fuera de EEUU podrán prestar mayor atención a lo que significará la presidencia de McCain, Clinton u Obama en términos de política y especialmente en asuntos exteriores

Correspondence - New Era or New Error? Technology and the Future of Deterrence

Ryan Snyder and Benoît Pelopidas respond to Keir A. Lieber and Daryl G. Press's spring 2017 article, “The New Era of Counterforce: Technological Change and the Future of Nuclear Deterrence.

Core components for effective infection prevention and control programmes: new WHO evidence-based recommendations

Abstract Health care-associated infections (HAI) are a major public health problem with a significant impact on morbidity, mortality and quality of life. They represent also an important economic burden to health systems worldwide. However, a large proportion of HAI are preventable through effective infection prevention and control (IPC) measures. Improvements in IPC at the national and facility level are critical for the successful containment of antimicrobial resistance and the prevention of HAI, including outbreaks of highly transmissible diseases through high quality care within the context of universal health coverage. Given the limited availability of IPC evidence-based guidance and standards, the World Health Organization (WHO) decided to prioritize the development of global recommendations on the core components of effective IPC programmes both at the national and acute health care facility level, based on systematic literature reviews and expert consensus. The aim of the guideline development process was to identify the evidence and evaluate its quality, consider patient values and preferences, resource implications, and the feasibility and acceptability of the recommendations. As a result, 11 recommendations and three good practice statements are presented here, including a summary of the supporting evidence, and form the substance of a new WHO IPC guideline

In Vivo Analysis of the Notch Receptor S1 Cleavage

A ligand-independent cleavage (S1) in the extracellular domain of the mammalian Notch receptor results in what is considered to be the canonical heterodimeric form of Notch on the cell surface. The in vivo consequences and significance of this cleavage on Drosophila Notch signaling remain unclear and contradictory. We determined the cleavage site in Drosophila and examined its in vivo function by a transgenic analysis of receptors that cannot be cleaved. Our results demonstrate a correlation between loss of cleavage and loss of in vivo function of the Notch receptor, supporting the notion that S1 cleavage is an in vivo mechanism of Notch signal control

Effective suppression of Dengue fever virus in mosquito cell cultures using retroviral transduction of hammerhead ribozymes targeting the viral genome

Outbreaks of Dengue impose a heavy economic burden on developing countries in terms of vector control and human morbidity. Effective vaccines against all four serotypes of Dengue are in development, but population replacement with transgenic vectors unable to transmit the virus might ultimately prove to be an effective approach to disease suppression, or even eradication. A key element of the refractory transgenic vector approach is the development of transgenes that effectively prohibit viral transmission. In this report we test the effectiveness of several hammerhead ribozymes for suppressing DENV in lentivirus-transduced mosquito cells in an attempt to mimic the transgenic use of these effector molecules in mosquitoes. A lentivirus vector that expresses these ribozymes as a fusion RNA molecule using an Ae. aegypti tRNAval promoter and terminating with a 60A tail insures optimal expression, localization, and activity of the hammerhead ribozyme against the DENV genome. Among the 14 hammerhead ribozymes we designed to attack the DENV-2 NGC genome, several appear to be relatively effective in reducing virus production from transduced cells by as much as 2 logs. Among the sequences targeted are 10 that are conserved among all DENV serotype 2 strains. Our results confirm that hammerhead ribozymes can be effective in suppressing DENV in a transgenic approach, and provide an alternative or supplementary approach to proposed siRNA strategies for DENV suppression in transgenic mosquitoes

Long-Term Infection and Vertical Transmission of a Gammaretrovirus in a Foreign Host Species

Increasing evidence has indicated natural transspecies transmission of gammaretroviruses; however, viral-host interactions after initial xeno-exposure remain poorly understood. Potential association of xenotropic murine leukemia virus-related virus (XMRV) in patients with prostate cancer and chronic fatigue syndrome has attracted broad interests in this topic. Although recent studies have indicated that XMRV is unlikely a human pathogen, further understanding of XMRV xenoinfection would allow in vivo modeling of the initial steps of gammaretroviral interspecies transmission, evolution and dissemination in a new host population. In this study, we monitored the long-term consequences of XMRV infection and its possible vertical transmission in a permissive foreign host, wild-derived Mus pahari mice. One year post-infection, XMRV-infected mice showed no notable pathological changes, while proviral DNA was detected in three out of eight mice. XMRV-infected mice remained seropositive throughout the study although the levels of gp70 Env- and p30 capsid-specific antibodies gradually decreased. When vertical XMRV transmission was assessed, no viremia, humoral immune responses nor endogenization were observed in nine offspring from infected mothers, yet one offspring was found PCR-positive for XMRV-specific sequences. Amplified viral sequences from the offspring showed several mutations, including one amino acid deletion in the receptor binding domain of Env SU. Our results therefore demonstrate long-term asymptomatic infection, low incidence of vertical transmission and limited evolution of XMRV upon transspecies infection of a permissive new host, Mus pahari