Temperature-dependence of Glu-AMS binding to GluRS.

<p>Integrated ITC curves of Glu-AMS (90 μM) binding to GluRS (9 μM) at different temperatures; 20°C (circles), 30°C (upside-down triangles), 37°C (squares).</p

Influence of tRNA on GluRS/Glu-AMS binding at 30°C.

<p>n = stoichiometry coefficient (number of moles of Glu-AMS bound per mole of GluRS monomer), ΔH_b = reaction enthalpy, ΔS_b = reaction entropy, ΔG_b = reaction energy (calculated with the formula ΔG_b = -RT Ln <i>K</i>_b, where R = 1.987 cal/mol·K).</p><p>All values and errors in this table were obtained by weighting by inverse variance [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121043#pone.0121043.ref032" target="_blank">32</a>], except for ΔG_b values and errors, obtained by simple average and standard error calculations.</p><p>Raw data and calculated values for each separated ITC runs are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121043#pone.0121043.s004" target="_blank">S1 Table</a>.</p><p>Influence of tRNA on GluRS/Glu-AMS binding at 30°C.</p

Temperature-dependance of the GluRS Glu-AMS interaction.

<p>n = stoichiometry coefficient (number of moles of Glu-AMS bound per mole of GluRS monomer), ΔH_b = reaction enthalpy, ΔS_b = reaction entropy, T = temperature, ΔG_b = reaction energy (calculated with the formula ΔG_b = -RT Ln <i>K</i>_b, where R = 1.987 cal/mol·K).</p><p>Injections of Glu-AMS at 90 μM were done in a starting concentration of 9 μM of GluRS.</p><p>Raw data and calculated values for each separated ITC runs are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121043#pone.0121043.s005" target="_blank">S2 Table</a>.</p><p>Temperature-dependance of the GluRS Glu-AMS interaction.</p

Glu-AMS binding to GluRS with/without tRNA.

<p>(a) Integrated ITC curves of Glu-AMS binding to GluRS with/without tRNA. Binding of Glu-AMS: to GluRS alone (circles), to GluRS with saturating concentration of tRNA^Glu in enriched total tRNA from <i>E</i>. <i>coli</i> (upside-down triangles), to GluRS with 11.23 µM tRNA^Phe from brewer’s yeast (squares). A duplicata of each tested condition is shown. The values shown in (b) were calculated from means of two distinct experiments reported in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121043#pone.0121043.t001" target="_blank">Table 1</a>, weighting by inverse variance [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121043#pone.0121043.ref032" target="_blank">32</a>].</p