Novel design for noise controlled semiconductor optical amplifier

The use of semiconductor optical amplifiers (SOA) in optical communications networks has so far been limited due to their inherent large noise figure (NF) compared to Erbium Doped Fibre Amplifiers. Therefore improvement of the noise performance of SOAs is critical to their widespread adoption in future networks. We propose to reduce the NF of the SOA by introducing a lasing cavity lateral to the axis of amplification of the device. The carrier density within the cavity is clamped at the lasing threshold. It is thus possible to engineer the carrier density distribution along the active waveguide by controlling the cavity design. According to our simulations, some of the cavity designs lead to a reduction of the noise figure in this novel SOA

Comparison of the Mechanisms of Drug Resistance among HIV, Hepatitis B, and Hepatitis C

Human immunodeficiency virus (HIV), hepatitis B virus (HBV), and hepatitis C virus (HCV) are the most prevalent deadly chronic viral diseases. HIV is treated by small molecule inhibitors. HBV is treated by immunomodulation and small molecule inhibitors. HCV is currently treated primarily by immunomodulation but many small molecules are in clinical development. Although HIV is a retrovirus, HBV is a double-stranded DNA virus, and HCV is a single-stranded RNA virus, antiviral drug resistance complicates the development of drugs and the successful treatment of each of these viruses. Although their replication cycles, therapeutic targets, and evolutionary mechanisms are different, the fundamental approaches to identifying and characterizing HIV, HBV, and HCV drug resistance are similar. This review describes the evolution of HIV, HBV, and HCV within individuals and populations and the genetic mechanisms associated with drug resistance to each of the antiviral drug classes used for their treatment

2022 Dugong Aerial Survey: Mission Beach to Moreton Bay

Project objectives: • Dugongs are of significant biodiversity value as the only extant species in the Family Dugongidae and one of only four species in the Order Sirenia, all of which are listed as vulnerable to extinction by the IUCN. • Australia has international responsibilities for dugong conservation, particularly in the Great Barrier Reef (GBR) region, where the dugong feeding grounds are listed as one of the World Heritage values of the region. • Dugongs have been monitored along the Queensland coast since the 1980s using a series of standardised aerial surveys. These surveys have provided long-term information on the distribution and abundance of dugongs, which has informed management and are a requirement of the Reef 2050 Long-Term Sustainability Plan (Reef 2050 Plan). The surveys have been loosely coordinated across jurisdictions and largely conducted at the same time of year at approximately five-year intervals. The areas adjacent to the Great Barrier Reef have been included to account for temporary migrations of dugongs across jurisdictional boundaries. • This report presents the result of an aerial survey for dugongs and large juvenile and adult marine turtles that was conducted in November-December 2022 in the coastal waters of Queensland from Mission Beach to the Queensland-New South Wales border. The survey is the latest in the time series of surveys conducted by James Cook University-TropWATER researchers since the 1980s. • The objectives of our study were to: 1. continue the time series of surveys for dugongs and large marine turtles. 2. to use the latest programming, modelling, and statistical advances to enhance our dugong distribution and abundance analysis. 3. engage with First Nations people across the surveyed area to: (1) raise awareness about dugong and sea turtle ecology and conservation issues, (2) seek interest from the communities in becoming involved in dugong survey work at different spatial scales, particularly aerial imagery surveys. 4. discuss new avenues for reducing uncertainty in the results for the surveys and the potential of new research tools for dugong monitoring in the future 5. provide advice to relevant management partners (GBRMPA, DCCEEW, and the Queensland Government) and Traditional Owners about the implications of the findings for the conservation, management, and monitoring of dugongs and large marine turtles1 in the southern GBR, Hervey Bay-GSS and Moreton Bay

mRNA concentrations of MIF in subcutaneous abdominal adipose cells are associated with adipocyte size and insulin action

Objective To determine whether the mRNA concentrations of inflammation response genes in isolated adipocytes and in cultured preadipocytes are related to adipocyte size and in vivo insulin action in obese individuals. Design Cross-sectional inpatient study. Subjects Obese Pima Indians with normal glucose tolerance. Measurements Adipocyte diameter (by microscope technique; n=29), expression of candidate genes (by quantitative real-time PCR) in freshly isolated adipocytes (monocyte chemoattractant protein [MCP] 1 and MCP2, macrophage inflammatory protein [MIP] 1α, MIP1β and MIP2, macrophage migration inhibitory factor [MIF], tumor necrosis factor alpha, interleukin [IL] 6 and IL8; n=22) and cultured preadipocytes (MCP1, MIP1α, MIF, IL6 and matrix metalloproteinase 2; n=33) from subcutaneous abdominal adipose tissue (by aspiration biopsy, n=34), body fat by dual-energy X-ray absorptiometry, glucose tolerance by 75-gram oral glucose tolerance test, and insulin action by euglycemic-hyperinsulinemic clamp (insulin infusion rate 40 mU/m2.min)(all n=34). Results MIF was the only gene whose expression in both freshly isolated adipocytes and cultured preadipocytes was positively associated with adipocytes diameter and negatively associated with peripheral and hepatic insulin action (all P<0.05). In multivariate analysis, the association between adipocyte MIF mRNA concentrations and adipocytes diameter was independent of percent body fat (P=0.03), whereas adipocyte MIF mRNA concentrations but not adipocytes diameter independently predicted peripheral insulin action. The mRNA expression concentrations of MIF gene in adipocytes were not associated with plasma concentrations of MIF, but were negatively associated with plasma adiponectin concentrations (P=0.004). In multivariate analysis, adipocyte MIF RNA concentrations (P=0.03) but not plasma adiponectin concentrations (P=0.4) remained a significant predictor of insulin action. Conclusions Increased expression of MIF gene in adipose cells may be an important link between obesity characterized by enlarged adipocytes and insulin resistance in normal glucose tolerant people

Improved retroviral suicide gene transfer in colon cancer cell lines after cell synchronization with methotrexate

<p>Abstract</p> <p>Background</p> <p>Cancer gene therapy by retroviral vectors is mainly limited by the level of transduction. Retroviral gene transfer requires target cell division. Cell synchronization, obtained by drugs inducing a reversible inhibition of DNA synthesis, could therefore be proposed to precondition target cells to retroviral gene transfer. We tested whether drug-mediated cell synchronization could enhance the transfer efficiency of a retroviral-mediated gene encoding herpes simplex virus thymidine kinase (HSV-<it>tk</it>) in two colon cancer cell lines, DHDK12 and HT29.</p> <p>Methods</p> <p>Synchronization was induced by methotrexate (MTX), aracytin (ara-C) or aphidicolin. Gene transfer efficiency was assessed by the level of HSV-TK expression. Transduced cells were driven by ganciclovir (GCV) towards apoptosis that was assessed using annexin V labeling by quantitative flow cytometry.</p> <p>Results</p> <p>DHDK12 and HT29 cells were synchronized in S phase with MTX but not ara-C or aphidicolin. In synchronized DHDK12 and HT29 cells, the HSV-TK transduction rates were 2 and 1.5-fold higher than those obtained in control cells, respectively. Furthermore, the rate of apoptosis was increased two-fold in MTX-treated DHDK12 cells after treatment with GCV.</p> <p>Conclusions</p> <p>Our findings indicate that MTX-mediated synchronization of target cells allowed a significant improvement of retroviral HSV-<it>tk </it>gene transfer, resulting in an increased cell apoptosis in response to GCV. Pharmacological control of cell cycle may thus be a useful strategy to optimize the efficiency of retroviral-mediated cancer gene therapy.</p

Effect of Floor Cooling on Behavior and Heart Rate of Late Lactation Sows Under Acute Heat Stress

Much U.S. swine production is in Köppen climate types classified as “hot-summer humid continental” and “humid subtropical.” As a result, farrowing sows are often exposed to temperatures above their upper critical temperature. This heat stress (HS) can affect sow welfare and productivity and have a negative economic impact. The study objective was to evaluate the impact of a cooling pad on sows' behavioral and heart rate responses to acute HS. Treatments were randomly allotted to ten multiparous sows to receive a constant cool water flow of 0.00 (CONTROL, n = 4), 0.25 (LOW, n = 2), 0.55 (MEDIUM, n = 2), or 0.85 (HIGH, n = 2) L/min for 100 min and replicated eight times, switching treatments so that each sow was exposed to each treatment. The cooling was initiated 1 h after the room reached 35°C for 100 min. Eating, drinking and nursing behaviors, postures, and heart rate were recorded before heating (Period 1), prior to cooling (Period 2), and during cooling (Period 3). There were no differences between LOW, MEDIUM, and HIGH flow rates for any periods on all behavioral and heart rate traits, so data were pooled (COOLED). There were no differences in any of the measures during Periods 1 and 2, except for the ratio of short term to long term heart rate variability (SD1:SD2) with higher values for CONTROL than COOLED sows in Period 2. During Period 3, CONTROL sows changed postures more frequently (11.5 ±1.6 vs. 5.1 ±1.6 changes per hour), spent more time drinker-pressing/drinking (4.4 ± 0.5 vs. 1.4 ± 0.4% of time), standing (6.6 ± 1.7 vs. 3.8 ± 1.6% of time), sitting (10.0 ± 1.2 vs. 4.0 ± 1.1), less time lying (83.0 ±1.8 vs. 92.0 ±1.7% of time), especially lying laterally (62.0 ± 5.6 vs. 75.0 ± 5.3% of time), than sows in all three cooling treatments (all P &lt; 0.001). Heart rate during Period 3 was lower for COOLED sows compared to the CONTROL sows (100.2 ± 3.4 vs. 119.0 ± 4.0 beat per min, P &lt; 0.001). Sows response to increased thermal load can be effectively reduced using water-cooled cooling pads, thereby improving sow comfort and welfare. The beneficial effects on behavior are noticeable from the lowest flow rate

A consumer’s guide to satellite remote sensing of multiple phytoplankton groups in the global ocean

Phytoplankton are composed of diverse taxonomical groups, which are manifested as distinct morphology, size and pigment composition. These characteristics, modulated by their physiological state, impact their light absorption and scattering, allowing them to be detected with ocean color satellite radiometry. There is a growing volume of literature describing satellite algorithms to retrieve information on phytoplankton composition in the ocean. This synthesis provides a review of current methods and a simplified comparison of approaches. The aim is to provide an easily comprehensible resource for non-algorithm developers, who desire to use these products, thereby raising the level of awareness and use of these products and reducing the boundary of expert knowledge needed to make a pragmatic selection of output products with confidence. The satellite input and output products, their associated validation metrics, as well as assumptions, strengths and limitations of the various algorithm types are described, providing a framework for algorithm organization to assist users and inspire new aspects of algorithm development capable of exploiting the higher spectral, spatial and temporal resolutions from the next generation of ocean color satellites

Noise controlled semiconductor optical amplifier based on lateral cavity laser

Experimental characterisation of a novel noise-controlled semiconductor optical amplifier (NCSOA) is presented. The design utilises grooves etched parallel to the active waveguide, at the output sections of the NCSOA, so as to induce lasing laterally to the propagation axis. This clamps the carrier density in a relevant region, allowing for the engineering of a specific longitudinal carrier density profile, corresponding to an improved noise figure performance. Results have demonstrated the effectiveness of carrier density profile engineering as a means of reducing the noise figure in semiconductor optical amplifier

Impact of bias current distribution on the noise figure and power saturation of a multi-contact semiconductor optical amplifier

We present an experimental investigation of a multi-contact semiconductor optical amplifier. This first-generation device allows for direct control of the carrier density profile along the length of the waveguide. This is used to control the device noise figure, with a minimum value of 5 dB observed at a gain of 15 dB for an optimum carrier density profile. The opposite carrier density profile results in an increase of the power saturation by 3 dB