Effect of boundaries on vacuum field fluctuations and radiation-mediated interactions between atoms

In this paper we discuss and review several aspects of the effect of boundary conditions and structured environments on dispersion and resonance interactions involving atoms or molecules, as well as on vacuum field fluctuations. We first consider the case of a perfect mirror, which is free to move around an equilibrium position and whose mechanical degrees of freedom are treated quantum mechanically. We investigate how the quantum fluctuations of the mirror's position affect vacuum field fluctuations for both a one-dimensional scalar and electromagnetic field, showing that the effect is particularly significant in the proximity of the moving mirror. This result can be also relevant for possible gravitational effects, since the field energy density couples to gravity. We stress that this interaction-induced modification of the vacuum field fluctuations can be probed through the Casimir-Polder interaction with a polarizable body, thus allowing to detect the effect of the mirror's quantum position fluctuations. We then consider the effect of an environment such as an isotropic photonic crystal or a metallic waveguide, on the resonance interaction between two entangled identical atoms, one excited and the other in the ground state. We discuss the strong dependence of the resonance interaction with the relative position of the atomic transition frequency with the gap of the photonic crystal in the former case, and with the cut-off frequency of waveguide in the latter.Comment: 8 pages, 2 figures, Proceedings of the Eighth International Workshop DICE 2016 Spacetime - Matter - Quantum Mechanic

Inhibitory interaction of the plasma membrane Na+/Ca2+ exchangers with the 14-3-3 proteins.

The three Na+/Ca2+ exchanger isoforms, NCX1, NCX2, and NCX3, contain a large cytoplasmic loop that is responsible for the regulation of activity. We have used 347 residues of the loop of NCX2 as the bait in a yeast two-hybrid approach to identify proteins that could interact with the exchanger and regulate its activity. Screening of a human brain cDNA library identified the epsilon and zeta isoforms of the 14-3-3 protein family as interacting partners of the exchanger. The interaction was confirmed by immunoprecipitation and in vitro binding experiments. The effect of the interaction on the homeostasis of Ca2+ was investigated by co-expressing NCX2 and 14-3-3epsilon in HeLa cells together with the recombinant Ca2+ probe aequorin; the ability of cells expressing both NCX2 and 14-3-3epsilon to dispose of a Ca2+ transient induced by an InsP3-producing agonist was substantially decreased, indicating a reduction of NCX2 activity. The 14-3-3epsilon protein also inhibited the NCX1 and NCX3 isoforms. In vitro binding experiments revealed that all three NCX isoforms interacted with multiple 14-3-3 isoforms. 14-3-3 was bound by both phosphorylated and nonphosphorylated NCX, but the phosphorylated form had much higher binding affinity

Nonlocal field correlations and dynamical Casimir-Polder forces between one excited- and two ground-state atoms

The problem of nonlocality in the dynamical three-body Casimir-Polder interaction between an initially excited and two ground-state atoms is considered. It is shown that the nonlocal spatial correlations of the field emitted by the excited atom during the initial part of its spontaneous decay may become manifest in the three-body interaction. The observability of this new phenomenon is discussed.Comment: 17 pages, 1 figure, sub. to Phys. Rev.

Testing the gamma-ray burst variability/peak luminosity correlation on a Swift homogeneous sample

We test the gamma-ray burst correlation between temporal variability and peak luminosity of the $\gamma$-ray profile on a homogeneous sample of 36 Swift/BAT GRBs with firm redshift determination. This is the first time that this correlation can be tested on a homogeneous data sample. The correlation is confirmed, as long as the 6 GRBs with low luminosity (<5x10^{50} erg s^{-1} in the rest-frame 100-1000 keV energy band) are ignored. We confirm that the considerable scatter of the correlation already known is not due to the combination of data from different instruments with different energy bands, but it is intrinsic to the correlation itself. Thanks to the unprecedented sensitivity of Swift/BAT, the variability/peak luminosity correlation is tested on low-luminosity GRBs. Our results show that these GRBs are definite outliers.Comment: Accepted for Publication in MNRAS. 10 pages, 5 figures, 3 table

Laryngectomized patients caregivers' life experience: A phenomenological study

Background: Laryngectomized patients often depend on their caregivers who have a central and difficult role in supporting them dealing with financial, social, and relational issues. In fact, they feel very responsible and committed, especially considering the time devoted to take care of their beloved. Besides, providing care induces caregivers high stress levels, emotional distress, anxiety and the fear of cancer recurrence or progress. Few studies investigated laryngectomized patients caregivers\u2019 life experience during both the whole course of illness and at the end of the treatment. Therefore, the purpose of our study was to explore, through a phenomenological approach, the lived-experience of primary family caregivers of laryngectomized patients undergoing radical surgery. Methods: Qualitative semi-structured and audiotaped interviews were held with 12 laryngectomized patients\u2019 primary family caregivers. Data were analysed using the descriptive phenomenological approach outlined by Colaizzi. Results: Three key themes emerged: the caregivers lived experience of illness; the change of caregivers\u2019 daily life and how they support their sick beloved. The experience of caregivers\u2019 lived relations changes from being a family member to a supportive carer, and the illness of their beloved negatively affects their psychological lived experience. Their perception of time and Quality of Life change as their perception of the future becomes uncertain. Finally, they feel guilty mainly because of the limited amount of time they can devote to their beloved. Conclusions: The study findings allowed to understand in depth how the presence of a laryngectomized person in the family may affect the life of the caregiver, even after the treatment phase. This suggests the need for healthcare professionals to support caregivers throughout the whole care journey and especially in dealing with the perception of time during the diagnosis and care phases. Further research should be conducted on factors contributing to time perception alteration and possible interventions to support caregivers to cope with it

Embryonic and foetal Islet-1 positive cells in human hearts are also positive to c-Kit

During embryogenesis, the mammalian heart develops from a primitive heart tube originating from two bilateral primary heart fields located in the lateral plate mesoderm. Cells belongings to the pre-cardiac mesoderm will differentiate into early cardiac progenitors, which express early transcription factors which are also common to the Isl-1 positive cardiac progenitor cells isolated from the developing pharyngeal mesoderm and the foetal and post-natal mice hearts. A second population of cardiac progenitor cells positive to c-Kit has been abundantly isolated from adult hearts. Until now, these two populations have been considered two different sets of progenitor cells present in the heart in different stages of an individual life. In the present study we collected embryonic, foetal and infant hearts, and we tested the hypotheses that c-Kit positive cells, usually isolated from the adult heart, are also present in the intra-uterine life and persist in the adult heart after birth, and that foetal Isl-1 positive cells are also positive to c-Kit. Using immunohistochemistry we studied the temporal distribution of Isl-1 positive and c-Kit/CD105 double positive cells, and by immunofluorescence and confocal analysis we studied the co-localization of c-Kit and Isl-1 positive cells. The results indicated that cardiomyocytes and interstitial cells were positive for c-Kit from the 9th to the 19h gestational week, that cells positive for both c-Kit and CD105 appeared in the interstitium at the 17h gestational week and persisted in the postnatal age, and that the Isl-1 positive cells were a subset of the c-Kit positive population

Use of platelet concentrate gel in second-intention wound healing: a case report

Background: Wound healing is a complex and dynamic process. Healing of acute and chronic wounds can be impaired by patient factors (that is, comorbidities) and/or wound factors (that is, infection). Regenerative medicine products, such as autologous/homologous platelet-rich plasma gel, may speed up the healing process. Autologous/homologous platelet-rich plasma is an advanced wound therapy used for hard-to-heal acute and chronic wounds. The cytokines and growth factors contained in platelet-rich plasma play a crucial role in the healing process. Case presentation: A 61-year-old Caucasian male patient, suffering from mental retardation following meningitis, with a transplanted kidney due to prior renal impairment, and under immunosuppressant therapy, was submitted to aneurysmectomy of his proximal left forearm arteriovenous fistula. A few days later, the patient came to our attention with substantial blood loss from the surgical site. The wound presented no signs of healing, and after fistula reparation and considering persistent infection of the surgical site (by methicillin-resistant Staphylococcus aureus), surgeons decided for second-intention healing. To favor healing, 10 mL homologous platelet concentrate gel was sequentially applied. After each application, wound was covered with nonadherent antiseptic dressing. After only seven applications of homologous platelet concentrate gel, wound completely recovered and no amputation was necessary. Conclusions: Topical application of homologous platelet-rich plasma gel in healing wound shows beneficial results in wound size reduction and induces granulation tissue formation. Platelet-rich plasma could be a safe and cost-effective treatment for managing the cutaneous wound healing process to shorten the recovery period and thereby improve patient quality of life