Supplementary data for article: Mileski, K. S.; Trifunović, S. S.; Ćirić, A. D.; Šakić, Ž. M.; Ristić, M. S.; Todorović, N. M.; Matevski, V. S.; Marin, P. D.; Tešević, V. V.; Džamić, A. M. Research on Chemical Composition and Biological Properties Including Antiquorum Sensing Activity of Angelica Pancicii Vandas Aerial Parts and Roots. Journal of Agricultural and Food Chemistry 2017, 65 (50), 10933–10949. https://doi.org/10.1021/acs.jafc.7b04202

Supporting information for: [https://doi.org/10.1021/acs.jafc.7b04202]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2572

Terpenes as Useful Markers in Differentiation of Natural Populations of Relict Pines Pinus heldreichii , P . nigra , and P . peuce

Comparative analysis of terpene diversity and differentiation of relict pines Pinus heldreichii, P. nigra, and P. peuce from the central Balkans was performed at the population level. Multivariate statistical analyses showed that the composition of needle terpenes reflects clear divergence among the pine species from different subgenera: P. peuce (subgenus Strobus) vs. P. nigra and P. heldreichii (subgenus Pinus). In addition, despite the described morphological similarities and the fact that P. nigra and P. heldreichii may spontaneously hybridize, our results indicated differentiation of their populations naturally growing in the same area. In accordance with recently proposed concept of 'flavonic evolution' in the genus Pinus, we assumed that the terpene profile of soft pine P. peuce, defined by high amounts of six monoterpenes, is more basal than those of hard pines P. nigra and P. heldreichii, which were characterized by high content levels of mainly sesquiterpenes. In order to establish precise positions of P. heldreichii, P. nigra and P. peuce within the taxonomic and phylogenetic tree, as well as develop suitable conservation strategies and future breeding efforts, it is necessary to perform additional morphological, biochemical, and genetic studies

Laserpitium ochridanum: antioxidant, antimicrobial and anti-quorum sensing activities against Pseudomonas aeruginosa

This study shows Laserpitium ochridanum essential oil composition, its antifungal potency, and possible antioxidant, antimicrobial and anti-quorum sensing activities of different extracts. Monoterpene hydrocarbons (40.9%) found to be the most abundant group of constituents in the oil. Sabinene (22.8%), viridiflorol (14.7%) and α-pinene (11.40%) were the main compounds of the oil. Ethanolic extract had the highest antioxidant capacity in DPPH and ABTS assays and was the richest in polyphenols. Microdilution method revealed the strongest antibacterial activity of ethanolic extracts in comparison to other tested extracts and streptomycin. L. ochridanum oil evidenced the best antifungal potential against used micromycetes. Results of anti-quorum sensing activity assay indicated high affection of aqueous extract in reduction of PAO1 pyocianin production (18.07%).Used samples possessed slight reduction of twitching and swimming mobility. Excellent antifungal oil activity, good antioxidant, antimicrobial and anti-quorum activity of L. ochridanum extracts was found. This study shows for the first time anti-guorum sensing activity against Pseudomonas aeruginosa of L. ochridanum, as well as its significant antioxidant potential. This study was conducted due to adventives of usage of natural products as efficient substitution for synthetic agents in treatment of various medical conditions

Chemical composition, antioxidative and antimicrobial activity of essential oil of Ocimum sanctum L.

Ocimum sanctum L. (Lamiaceae) sin. Ocimum tenuiflorum L. or Tulsi basil is a plant originating from the tropical and subtropical areas of India. It is used in both the traditional and official medicine in India. Tulsi is a type of basil that is insufficiently explored and studied in Europe. The goal of this paper is to determine the chemical composition, antioxidative, and antimicrobial activity of the essential oil Ocimum sanctum L. grown in Serbia. The quantity of essential oil in 100 g of herb (v/w) is 0.68%, with 41 components identified in the tested essential oil. The most represented chemical group were sesquiturpene hydrocarbonates with 80.47%. Other groups were much less represented. Sesquiturpene hydrocarbonate β-cariophyllene is a predominant component in the essential oil with 63.80%. The quantity of tested essential oil needed to achieve 50% of inhibition of DPPH radicals is 0.35 µg/ml, and it has high potential to neutralize free radicals. The essential oil exhibited antibacterial activity to all tested strains of bacteria, both Gram-positive and Gram-negative. It affected all strains in an inhibitory way in the interval 0.34-41.50 µl/ml, and in a bactericide way within the range 22.50-124.5 µl/ml. The most sensitive strains of bacteria were Salmonella typhimurium and Escherichia coli, while Listeria monocytogenes and Enterococus faecalis showed greatest resistance. The essential oil exhibited antifungal activity on all tested fungi. It affected all tested fungi in an inhibitory way in the interval 4.42-8.83 µl/ml, and in a microbicide way within the range 10.00-50.00 µl/ml. The most sensitive fungi are: Aspergillus ochraceus, Penicillium ochrochloron and Penicilium funiculosum, while the most resistant one is Aspergillus niger. The tested basil essential oil Ocimum sanctum demonstrated significant antioxidative and antimicrobial effect and may be used as a raw material in food, pharmaceutical and chemical industries.U radu su prikazani rezultati ispitivanja hemijskog sastava, antioksidativne i antimikrobne aktivnosti etarskog ulja Ocimum sanctum L. gajenog u Srbiji. Količina etarskog ulja u suvoj herbi prosečno iznosi 0,68%. U njemu je identifikovana 41 komponenta. Najzastupljenija hemijska grupa su seskviterpenski ugljovodonici sa 80,47%. Seskviterpenski ugljovodonik β-kariofilen je sa 63,80% dominantna komponenta u etarskom ulju. Količina etarskog ulja koja je potrebna da se ostvari 50% inhibicije DPPH radikala iznosi 0,35 |µg/ml te ono poseduje visoku sposobnost neutralizacije slobodnih radikala. Etarsko ulje je ispoljilo antibakterijsku aktivnost na sve testirane Gram-pozitivne i Gram-negativne bakterijske sojeve. Ono je delovalo na sve sojeve inhibitorno u intervalu 0,34-41,50 |µl/ml i baktericidno u opsegu 22,50-124,50 |µl/ml. Etarsko ulje je ispoljilo i antifungalnu aktivnost na sve testirane gljive i delovalo je inhibitorno u intervalu 4,42-8,83 |µl/ml i mikrobicidno u opsegu 10,00-50,00 |µl/ml.Projekat ministarstva br. III 46001, br. OI 173015 i br. OI 17303

Chemical composition, antioxidative and antimicrobial activity of essential oil of Ocimum sanctum L.

Ocimum sanctum L. (Lamiaceae) sin. Ocimum tenuiflorum L. or Tulsi basil is a plant originating from the tropical and subtropical areas of India. It is used in both the traditional and official medicine in India. Tulsi is a type of basil that is insufficiently explored and studied in Europe. The goal of this paper is to determine the chemical composition, antioxidative, and antimicrobial activity of the essential oil Ocimum sanctum L. grown in Serbia. The quantity of essential oil in 100 g of herb (v/w) is 0.68%, with 41 components identified in the tested essential oil. The most represented chemical group were sesquiturpene hydrocarbonates with 80.47%. Other groups were much less represented. Sesquiturpene hydrocarbonate β-cariophyllene is a predominant component in the essential oil with 63.80%. The quantity of tested essential oil needed to achieve 50% of inhibition of DPPH radicals is 0.35 µg/ml, and it has high potential to neutralize free radicals. The essential oil exhibited antibacterial activity to all tested strains of bacteria, both Gram-positive and Gram-negative. It affected all strains in an inhibitory way in the interval 0.34-41.50 µl/ml, and in a bactericide way within the range 22.50-124.5 µl/ml. The most sensitive strains of bacteria were Salmonella typhimurium and Escherichia coli, while Listeria monocytogenes and Enterococus faecalis showed greatest resistance. The essential oil exhibited antifungal activity on all tested fungi. It affected all tested fungi in an inhibitory way in the interval 4.42-8.83 µl/ml, and in a microbicide way within the range 10.00-50.00 µl/ml. The most sensitive fungi are: Aspergillus ochraceus, Penicillium ochrochloron and Penicilium funiculosum, while the most resistant one is Aspergillus niger. The tested basil essential oil Ocimum sanctum demonstrated significant antioxidative and antimicrobial effect and may be used as a raw material in food, pharmaceutical and chemical industries.U radu su prikazani rezultati ispitivanja hemijskog sastava, antioksidativne i antimikrobne aktivnosti etarskog ulja Ocimum sanctum L. gajenog u Srbiji. Količina etarskog ulja u suvoj herbi prosečno iznosi 0,68%. U njemu je identifikovana 41 komponenta. Najzastupljenija hemijska grupa su seskviterpenski ugljovodonici sa 80,47%. Seskviterpenski ugljovodonik β-kariofilen je sa 63,80% dominantna komponenta u etarskom ulju. Količina etarskog ulja koja je potrebna da se ostvari 50% inhibicije DPPH radikala iznosi 0,35 |µg/ml te ono poseduje visoku sposobnost neutralizacije slobodnih radikala. Etarsko ulje je ispoljilo antibakterijsku aktivnost na sve testirane Gram-pozitivne i Gram-negativne bakterijske sojeve. Ono je delovalo na sve sojeve inhibitorno u intervalu 0,34-41,50 |µl/ml i baktericidno u opsegu 22,50-124,50 |µl/ml. Etarsko ulje je ispoljilo i antifungalnu aktivnost na sve testirane gljive i delovalo je inhibitorno u intervalu 4,42-8,83 |µl/ml i mikrobicidno u opsegu 10,00-50,00 |µl/ml.Projekat ministarstva br. III 46001, br. OI 173015 i br. OI 17303

Chemical profile of Taxodium distichum winter cones

This work is concerned with the chemical profile of Taxodium distichum winter cones. The extract obtained after maceration in absolute ethanol was subjected to qualitative analysis by gas chromatography/mass spectrometry and quantification was done by gas chromatography/ flame ionization detector. The chromatogram revealed the presence of 53 compounds, of which 33 compounds were identified. The extract contained oxygenated monoterpenes (12.42%), sesquiterpenes (5.18%), oxygenated sesquiterpenes (17.41%), diterpenes (1.15%), and oxygenated diterpenes (30.87%), while the amount of retinoic acid was 0.32%. Monoacylglycerols were detected in the amount of 4.32%. The most abundant compounds were: caryophyllene oxide (14.27%), 6,7-dehydro-ferruginol (12.49%), bornyl acetate (10.96%), 6- deoxy-taxodione (9.50%) and trans-caryophyllene (4.20%)

The combined extraction of sage (Salvia officinalis L.): Ultrasound followed by supercritical CO2 extraction

A wide spectrum of phytochemicals could be isolated from sage (Salvia officinalis L) using different extraction or distillation technique: the supercritical fluid extraction (SFE), the volatiles compounds (monoterpenes and sesquiterpenes) isolation using hydrodistillation or higher molecular compounds with Soxhlet extraction or ultrasound-assisted extraction. The combination of ultrasound-assisted extraction followed by re-extraction of obtained extract with supercritical CO2 was performed in this study. The goal of performed investigation was to concentrate diterpenes present in sage extract which are generally considered to be responsible for antioxidant activity of extracted compounds. The fractionation using the supercritical CO2, and different combination of the ultrasound-assisted solvent extractions (water-ethanol mixture or only water) followed by supercritical CO2 re-extraction of obtained extract or treated plant material were analyzed and compared. Based on the results of these investigations it could be proposed the best extraction procedure: the ultrasound pretreatment of plant material with distilled water and re-extraction of plant material (residue) using supercritical CO2. That procedure gives two valuable products: the ultrasound extract which is rich in sugars and possess the immunomodulatory activity and supercritical extract which is rich in diterpenes and sesquiterpenes

Chemical composition and antifungal activity of the essential oil from Juniperus excelsa berries

Essential oil of Juniperus excelsia Bieb M (Cupressaceae) was' evaluated for antifungal activities against 17 micromycetes (Alternaria alternata, Aspergillus niger, A. ochraceus, A. versicolor A. flavus, A. terreus, Cladosporium cladosporioides, Fusarium tricinctum, Penicillium ochrocloron, P funiculosum, Phomopsis helianthi, Trichoderma viride, Trichophyton mentagrophytes, T rubrum, T tonsurans, Epidermophyton floccosum, Microsporum canis). Among tested fungal species were the food poisoning species, plant, animal, and human pathogen. The essential oil showed antifungal activity.nul

Chemical composition and antifungal activities of essential oils from leaves, calyx and corolla of Salvia brachyodon Vandas

The chemical composition and antifungal activity of the corolla, calyx and leaf oils of Salvia brachyodon were analyzed by GC and GUMS. The oils were hydrodistilled from the leaves, corolla and calyx, separately. The leaf oil contained 1,8-cineole (33.0%), alpha-pinene (12.7%), camphene (12.5%) and beta-pinene (11.3%) as the most abundant constituents. The oil isolated from the calyx contained beta-pinene (27.6%), alpha-pinene (16.2%), camphene (13.7%), borneol (13.2%) and camphor (12.4%) as major components, while in the corolla oil the main components were beta-pinene (31.7%), borneol (12.8%), alpha-pinene (11.8%), camphene (6.7%) and alpha-pinene (11.8%). The last sample, however, was the most complex, because of the presence of higher concentration of sesquiterpenes and presence of diterpenes, which were not recorded in the other samples investigated. Antifungal activities of the essential oils were determined by the microdilution method against Aspergillus niger, Trichophyton mentagrophytes and Candida albicans. The oil from the calyx possessed the best antifungal activity against all the fungal species tested.nul